LOCUS       NM_001100517            3363 bp    mRNA    linear   ROD 20-OCT-2024
DEFINITION  Rattus norvegicus alanyl-tRNA synthetase 1 (Aars1), mRNA.
ACCESSION   NM_001100517 XM_001077503 XM_214690
VERSION     NM_001100517.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Vo,M.N., Terrey,M., Lee,J.W., Roy,B., Moresco,J.J., Sun,L., Fu,H.,
            Liu,Q., Weber,T.G., Yates,J.R. 3rd, Fredrick,K., Schimmel,P. and
            Ackerman,S.L.
  TITLE     ANKRD16 prevents neuron loss caused by an editing-defective tRNA
            synthetase
  JOURNAL   Nature 557 (7706), 510-515 (2018)
   PUBMED   29769718
  REMARK    Erratum:[Nature. 2018 Aug;560(7720):E35. doi:
            10.1038/s41586-018-0271-3. PMID: 29925958]
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Nakayama,T., Wu,J., Galvin-Parton,P., Weiss,J., Andriola,M.R.,
            Hill,R.S., Vaughan,D.J., El-Quessny,M., Barry,B.J., Partlow,J.N.,
            Barkovich,A.J., Ling,J. and Mochida,G.H.
  TITLE     Deficient activity of alanyl-tRNA synthetase underlies an autosomal
            recessive syndrome of progressive microcephaly, hypomyelination,
            and epileptic encephalopathy
  JOURNAL   Hum Mutat 38 (10), 1348-1354 (2017)
   PUBMED   28493438
REFERENCE   3  (bases 1 to 3363)
  AUTHORS   Sun,L., Gomes,A.C., He,W., Zhou,H., Wang,X., Pan,D.W., Schimmel,P.,
            Pan,T. and Yang,X.L.
  TITLE     Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a
            G4:U69 Base Pair
  JOURNAL   J Am Chem Soc 138 (39), 12948-12955 (2016)
   PUBMED   27622773
REFERENCE   4  (bases 1 to 3363)
  AUTHORS   Liu,Y., Satz,J.S., Vo,M.N., Nangle,L.A., Schimmel,P. and
            Ackerman,S.L.
  TITLE     Deficiencies in tRNA synthetase editing activity cause
            cardioproteinopathy
  JOURNAL   Proc Natl Acad Sci U S A 111 (49), 17570-17575 (2014)
   PUBMED   25422440
REFERENCE   5  (bases 1 to 3363)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   6  (bases 1 to 3363)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 3363)
  AUTHORS   Guo,M., Chong,Y.E., Shapiro,R., Beebe,K., Yang,X.L. and Schimmel,P.
  TITLE     Paradox of mistranslation of serine for alanine caused by AlaRS
            recognition dilemma
  JOURNAL   Nature 462 (7274), 808-812 (2009)
   PUBMED   20010690
REFERENCE   8  (bases 1 to 3363)
  AUTHORS   Lee,J.W., Beebe,K., Nangle,L.A., Jang,J., Longo-Guess,C.M.,
            Cook,S.A., Davisson,M.T., Sundberg,J.P., Schimmel,P. and
            Ackerman,S.L.
  TITLE     Editing-defective tRNA synthetase causes protein misfolding and
            neurodegeneration
  JOURNAL   Nature 443 (7107), 50-55 (2006)
   PUBMED   16906134
REFERENCE   9  (bases 1 to 3363)
  AUTHORS   Dignam,J.D., Dignam,S.S. and Brumley,L.L.
  TITLE     Alanyl-tRNA synthetase from Escherichia coli, Bombyx mori and Ratus
            ratus. Existence of common structural features
  JOURNAL   Eur J Biochem 198 (1), 201-210 (1991)
   PUBMED   2040280
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473972.1.
            
            [WARNING] On Oct 26, 2024 this sequence was replaced by
            NM_001100517.2.
            
            On or before Oct 22, 2008 this sequence version replaced
            XM_001077503.1, XM_214690.4.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132263,
                              SAMD00132265 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..3363
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /note="alanyl-tRNA synthetase 1"
                     /db_xref="GeneID:292023"
                     /db_xref="RGD:1304832"
     exon            1..67
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            68..237
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..3000
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /EC_number="6.1.1.7"
                     /note="alanyl-tRNA synthetase, cytoplasmic; alaRS; protein
                     lactyltransferase AARS1"
                     /codon_start=1
                     /product="alanine--tRNA ligase, cytoplasmic"
                     /protein_id="NP_001093987.1"
                     /db_xref="GeneID:292023"
                     /db_xref="RGD:1304832"
                     /translation="MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANA
                     GMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLG
                     SWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLG
                     LDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLV
                     FIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGA
                     RPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRY
                     SHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRI
                     LDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQL
                     KSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLA
                     LRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNA
                     QVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQ
                     KGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGL
                     RAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIV
                     TEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADL
                     GEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQP
                     LVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGL
                     KASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN"
     exon            238..426
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            427..572
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            573..764
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            765..909
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1055
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1164
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1315
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1440
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1585
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1764
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1878
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2085
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..2270
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2379
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2493
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2613
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2700
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2814
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..3363
                     /gene="Aars1"
                     /gene_synonym="Aars"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggtcactt ttcatcagag cgctctgatt gggtggtgag gtacgggtct ggcaccggtt
       61 gtggcggtta gttccctgag gagattttca gagatggact ccactctaac agcacgagag
      121 atccgagagc ggtttataaa ttatttcaaa cgaaatgagc acacctatgt ccactcctcc
      181 gccaccatcc ctctggacga ccccaccttg ctcttcgcca acgcaggcat gaaccagttc
      241 aaacccatct tcctaaacac agtagaccct tctcacccca tggcaaagct gagcagagct
      301 gccaataccc agaagtgcat ccgagccggg ggcaaacaca atgacctgga tgacgtgggc
      361 aaagatgtct accaccacac cttctttgag atgctgggct cctggtcttt tggggactat
      421 tttaaggaat tggcatgtaa gatggctctg gaacttctta cccaggagtt tggtattcca
      481 gttgaaagac tctatgttac ttactttggt ggggatgagg cagccggcct agagccagat
      541 ctggagtgta gacagatctg gcaaaattta ggactggatg aagccaaaat tctccctggc
      601 aacatgaagg acaacttctg ggagatgggt gacacgggcc cctgtggtcc ctgcagtgag
      661 atccactatg accgcattgg tggcagggac gctgcacacc ttgtcaacca ggatgacccc
      721 aatgtgctag agatctggaa tctcgtgttc atccaatata acagggaatc tgatggtgtt
      781 ctgaaacctc tccccaagaa aagcattgac acagggatgg gcctggagag attggtgtct
      841 gtgctgcaga acaagatgtc caactatgac actgaccttt tcgttcctta cttcgaagcc
      901 attcagaagg gtacaggcgc ccggccgtat actgggaagg ttggtgctga ggacactgat
      961 ggaattgaca tggcctacag ggttctggct gaccacgccc ggaccatcac tgtggcgctg
     1021 gctgatggcg ggcgacctga caacacaggt cgggggtatg tgctgagacg gatccttcgc
     1081 cgagctgttc gctattccca cgagaaactg aacgccagca ggggtttctt cgccacatta
     1141 gttgatgttg tcgttcaatc cctgggagac gcctttcctg agctgaagaa ggacccagat
     1201 atggtgaagg acatcattaa tgaagaagag gtacagtttc tcaagactct cagcagaggg
     1261 cggcgcatcc tggaccggaa aattcagagc ttaggagact gccaaaccat cccaggtgat
     1321 accgcttggc tcctctatga tacttacgga ttcccagtgg atctcactgg attgattgct
     1381 gaagagaagg gcctggtggt agatatggat ggctttgaag aagagaggaa actggcccag
     1441 ctgaagtcac agggcaaggg ggcagggggc gaagacctca tcatgctgga catttatgcg
     1501 attgaagaac tccgggcaaa gggtttggag gccacagatg attctccaaa gtataactac
     1561 cattcagact ccagtggtag ctatgtgttt gaatgtacag tggctaccgt gcttgctctg
     1621 cgtagggaaa agatgtttgt ggacgaggtg gtcactggcc aggagtgcgg agtggtgctg
     1681 gacaagacct gtttctatgc cgagcaagga gggcagatct tcgatgaagg ctacttggtg
     1741 aaggtcgacg acagcagtga ggataaaact gagtttacag tgaagaatgc tcaggtccga
     1801 ggcgggtatg tgctgcacat aggaacaata tatggtaacc tgagagtggg ggatcaagtc
     1861 cggctgttca ttgacgagcc ccgacgaaga cctgtcatga gcaaccatac agctacacac
     1921 atcctgaact tcgccttgcg ctcggtgctt ggggatgcag accagaaagg ttcactggtt
     1981 gctcctgacc gccttcggtt tgacttcact gccaagggag ccatgtccac ggagcaaatc
     2041 aagaagactg aggagattgt caatggcatg attgaggcag ctaagcctgt ctacaccctg
     2101 gattgtcccc tggctgcagc aaaagccatc cagggccttc gagctgtatt tgatgagacc
     2161 tatcctgacc ctgtgcgagt tgtctccatt ggagtcccag tgtcagaact gctagatgac
     2221 ccctccgggc ctgccggctc ccttacttct gttgagtttt gtggaggaac acatctacgg
     2281 aattctagcc atgcaggagc tttcgtgata gtgactgaag aagccattgc caagggtatc
     2341 cgcaggattg ttgctgtcac aggtgctgaa gcccagaagg ccctcaggaa gtcagaaacc
     2401 ttgaagaaat ctctctcggc catggaggtc aaagtgaagg cccagtctgc acccaacaag
     2461 gatgtgcaga aggaaattgc tgaccttggt gaggtcctgg ccactgcagt cattccccag
     2521 tggcagaaag atgaacaacg ggagactttg aaatccctga agaaagtcat ggatgaccta
     2581 gaccgggcca gcaaagccga tgtccagaag cgagtgttag agaagactaa gcagctgatt
     2641 gacagcaacc ccaaccagcc tctggtcatc ctggagatgg agagcggtgc atcggccaag
     2701 gccctgaatg aagctttgaa gcttttcaag acacattctc ctcagacatc tgcaatgctc
     2761 ttcacagtag acaatgaggc tggcaagatc acatgcttgt gtcaggttcc tcagaatgca
     2821 gccaaccggg ggctaaaagc cagcgagtgg gtccagcagg tttcaggcct gatggatggt
     2881 aaaggtggtg ggaaggacat gtctgcccag gccacaggca agaacgtggg ctgccttcag
     2941 gaagcactgc agctggctac ttcctttgcc cagctccgtc tcggagatgt aaagaactga
     3001 tgggagggaa gccctctcag gaaagcatct ccccgacaac cagtcatgtt caggtgcagg
     3061 agtctagcca ggagctctcc tcctgccacg aagacatttg agtcttggga cctttcagca
     3121 gccctccgtc ctgatccagc agtaactgga acgcgcctgg agctgtcctg tgattcattg
     3181 cccctttcca ttctgctctg agccataatg tcagcgccat ctgtctaaag tcactaccca
     3241 ggaatgctac ctaacatcct gatcacgtct agatgaggtc actctgccaa cctgaggctt
     3301 ctaggtctgc aagtgggaag gaagccttcc actgcagaaa ataaaggacc atgtgcaata
     3361 cta
//
