LOCUS       NM_023104               3191 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus acetoacetyl-CoA synthetase (Aacs), mRNA.
ACCESSION   NM_023104
VERSION     NM_023104.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Wu,D.M., He,Z., Chen,T., Liu,Y., Ma,L.P. and Ping,J.
  TITLE     DNA hypermethylation of acetoacetyl-CoA synthetase contributes to
            inhibited cholesterol supply and steroidogenesis in fetal rat
            adrenals under prenatal nicotine exposure
  JOURNAL   Toxicology 340, 43-52 (2016)
   PUBMED   26776438
  REMARK    GeneRIF: Prenatal nicotine exposure can cause DNA hypermethylation
            of the AACS promoter in the rat fetal adrenal.
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Yamasaki,M., Hasegawa,S., Yamanaka,H., Narishima,R. and Fukui,T.
  TITLE     Ketone body utilization is regulated by male-specific factors in
            rat subcutaneous adipocytes
  JOURNAL   Exp Clin Endocrinol Diabetes 117 (4), 170-174 (2009)
   PUBMED   19085696
REFERENCE   3  (bases 1 to 3191)
  AUTHORS   Narishima,R., Yamasaki,M., Hasegawa,S. and Fukui,T.
  TITLE     Genetic obesity affects neural ketone body utilization in the rat
            brain
  JOURNAL   Obesity (Silver Spring) 17 (3), 611-615 (2009)
   PUBMED   19219059
  REMARK    GeneRIF: As acetoacetyl-CoA synthetase was expressed in neural-like
            cells, ketone bodies are assumed to be utilized for the synthesis
            of lipidic substances and to cause metabolic disorders in the
            nervous system of genetically obese rats.
REFERENCE   4  (bases 1 to 3191)
  AUTHORS   MacDonald,M.J., Smith,A.D. 3rd, Hasan,N.M., Sabat,G. and
            Fahien,L.A.
  TITLE     Feasibility of pathways for transfer of acyl groups from
            mitochondria to the cytosol to form short chain acyl-CoAs in the
            pancreatic beta cell
  JOURNAL   J Biol Chem 282 (42), 30596-30606 (2007)
   PUBMED   17724028
REFERENCE   5  (bases 1 to 3191)
  AUTHORS   Yamasaki,M., Hasegawa,S., Suzuki,H., Hidai,K., Saitoh,Y. and
            Fukui,T.
  TITLE     Acetoacetyl-CoA synthetase gene is abundant in rat adipose, and
            related with fatty acid synthesis in mature adipocytes
  JOURNAL   Biochem Biophys Res Commun 335 (1), 215-219 (2005)
   PUBMED   16055091
  REMARK    GeneRIF: AACS in adipose tissue plays an important role in
            utilizing ketone body for the fatty acid-synthesis during adipose
            tissue development
REFERENCE   6  (bases 1 to 3191)
  AUTHORS   Salam,W.H., Wilcox,H.G., Cagen,L.M. and Heimberg,M.
  TITLE     Stimulation of hepatic cholesterol biosynthesis by fatty acids.
            Effects of oleate on cytoplasmic acetoacetyl-CoA thiolase,
            acetoacetyl-CoA synthetase and hydroxymethylglutaryl-CoA synthase
  JOURNAL   Biochem J 258 (2), 563-568 (1989)
   PUBMED   2565110
REFERENCE   7  (bases 1 to 3191)
  AUTHORS   Wong,G.A., Bergstrom,J.D. and Edmond,J.
  TITLE     Acetoacetyl-CoA ligase activity in the isolated rat hepatocyte:
            effects of 25-hydroxycholesterol and high density lipoprotein
  JOURNAL   Biosci Rep 7 (3), 217-224 (1987)
   PUBMED   3663886
REFERENCE   8  (bases 1 to 3191)
  AUTHORS   Ito,M., Fukui,T., Saito,T. and Tomita,K.
  TITLE     Acetoacetyl-CoA synthetase specific activity and concentration in
            rat tissues
  JOURNAL   Biochim Biophys Acta 876 (2), 280-287 (1986)
   PUBMED   3082367
REFERENCE   9  (bases 1 to 3191)
  AUTHORS   Bergstrom,J.D., Wong,G.A., Edwards,P.A. and Edmond,J.
  TITLE     The regulation of acetoacetyl-CoA synthetase activity by modulators
            of cholesterol synthesis in vivo and the utilization of
            acetoacetate for cholesterogenesis
  JOURNAL   J Biol Chem 259 (23), 14548-14553 (1984)
   PUBMED   6501306
REFERENCE   10 (bases 1 to 3191)
  AUTHORS   Robinson,A.M. and Williamson,D.H.
  TITLE     Utlization of D-3-hydroxy[3-14C]butyrate for lipogenesis in vivo in
            lactating rat mammary gland
  JOURNAL   Biochem J 176 (2), 635-638 (1978)
   PUBMED   33671
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 26, 2020 this sequence version replaced NM_023104.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB026291.1, BC061803.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-241               JAXUCZ010000012.1  36817534-36817774   c
            242-345             JAXUCZ010000012.1  36809479-36809582   c
            346-466             JAXUCZ010000012.1  36807631-36807751   c
            467-580             JAXUCZ010000012.1  36802834-36802947   c
            581-678             JAXUCZ010000012.1  36798670-36798767   c
            679-793             JAXUCZ010000012.1  36791276-36791390   c
            794-875             JAXUCZ010000012.1  36790968-36791049   c
            876-1023            JAXUCZ010000012.1  36787371-36787518   c
            1024-1104           JAXUCZ010000012.1  36784781-36784861   c
            1105-1229           JAXUCZ010000012.1  36783581-36783705   c
            1230-1294           JAXUCZ010000012.1  36780746-36780810   c
            1295-1417           JAXUCZ010000012.1  36780483-36780605   c
            1418-1531           JAXUCZ010000012.1  36779384-36779497   c
            1532-1657           JAXUCZ010000012.1  36778797-36778922   c
            1658-1727           JAXUCZ010000012.1  36778027-36778096   c
            1728-1786           JAXUCZ010000012.1  36777747-36777805   c
            1787-1989           JAXUCZ010000012.1  36776805-36777007   c
            1990-3191           JAXUCZ010000012.1  36774471-36775672   c
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q14"
     gene            1..3191
                     /gene="Aacs"
                     /note="acetoacetyl-CoA synthetase"
                     /db_xref="GeneID:65984"
                     /db_xref="RGD:708522"
     exon            1..241
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..2127
                     /gene="Aacs"
                     /EC_number="6.2.1.16"
                     /note="AcAc-CoA ligase; acetoacetyl-CoA ligase;
                     acetoacetate-CoA ligase"
                     /codon_start=1
                     /product="acetoacetyl-CoA synthetase"
                     /protein_id="NP_075592.1"
                     /db_xref="GeneID:65984"
                     /db_xref="RGD:708522"
                     /translation="MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLAL
                     GNYDDLYHWSVRSYSDFWAEFWKFSGIVCSRMYDEVVDTSKGIADVPEWFRGSRLNYA
                     ENLLRHKENDRVALYVAREGREEIAKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYL
                     PNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHG
                     HLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSMFLDDFLASGTGAQAPQLEF
                     EQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHVLHGNMTSSDILLYYTTVG
                     WMMWNWMVSALATGASLVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKD
                     MKPMETHNLHTLHTILSTGSPLKAQSYEYVYRCIKSTVLLGSISGGTDIISCFMGQNS
                     SIPVYKGEIQARNLGMAVEAWDEEGKTVWGASGELVCTKPIPCQPTHFWNDENGSKYR
                     KAYFSKYPGVWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVEAFDEV
                     EDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKHIRDAIRLGLSARHVPSLILETQ
                     GIPYTINGKKVEVAVKQVIAGKTVEHRGAFSNPESLDLYRDIPELQDF"
     exon            242..345
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            346..466
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            467..580
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            581..678
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            679..793
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            794..875
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1023
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1104
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1229
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1294
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1417
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1531
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1657
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1727
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1786
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1989
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..3191
                     /gene="Aacs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tctcgcgctg tggttcgtcc gcgccacgct gagccgctcc acgccttgcg ctctccgctg
       61 tctccgcagc taaagcccgg gcagccccgg ccacgcagct ccgcaaccat gtccaagctg
      121 gcacggctcg agcgcgagga gatcatggag tgccaggtga tgtgggagcc tgacagcaag
      181 aaggacacgc agatggaccg cttccgggcg gccgtgggta ctgcctgcgg cctggcgctt
      241 gggaattacg atgacttata ccactggtct gtccggtcgt attcagactt ctgggctgag
      301 ttctggaagt tcagtggaat tgtctgctct cgcatgtatg atgaggttgt ggacacatcc
      361 aaaggaattg cagatgtccc tgagtggttc agaggcagcc gcctcaacta tgcagagaac
      421 cttctgcggc acaaggagaa cgacagagtc gccctttacg tggcccggga aggcagagag
      481 gagattgcga aggtgacttt cgaagagctt cggcagcagg tggctctgtt tgcagccgcc
      541 atgaggaaga tgggcgtgaa gaaaggggac cgtgtggtcg gttatctccc caacagtgcc
      601 catgccgtgg aggccatgct ggctgctgcc agtattggag ccatttggag ttctacctca
      661 ccagactttg gtgtgaatgg tgtcctggac cgcttttctc aaattcagcc gaaacttatc
      721 ttctcggtgg aagctgtggt ctacaacggc aaggaacacg gccacctgga gaagctgcag
      781 cgagtcgtga aaggacttcc tgaccttcag cgagtggtgc tgatccccta tgtcctccca
      841 agggagaaga tagacatttc caagatcccc aacagcatgt ttctggatga cttcctggca
      901 agcgggacag gtgcgcaggc accacagctc gagtttgaac agctgccctt cagccatccc
      961 ctgttcatca tgttctcctc gggcacgaca ggagcgccca agtgcatggt gcactctgct
     1021 gggggcaccc tcatccagca cctgaaggag cacgtgctac atggcaacat gacaagcagt
     1081 gacatcctgc tctactacac cacggtcggc tggatgatgt ggaactggat ggtgtcagcg
     1141 ctggccacag gagcatcctt ggttctgtac gatggctccc cgctggttcc aacacccaat
     1201 gtgttgtggg accttgtgga caggatagga atcaccatcc tgggaacggg agccaagtgg
     1261 ctgtcagtgc tggaggagaa ggacatgaag ccgatggaaa ctcacaacct ccacacgctc
     1321 cacacgatcc tgtccaccgg ctcgccactg aaagcccaga gctatgagta tgtgtacaga
     1381 tgcatcaaga gcaccgtgct cctcggctcc atctcaggtg gcactgacat catctcctgt
     1441 ttcatgggcc agaactcatc tattcctgtg tacaagggtg agatccaagc ccggaacctc
     1501 ggcatggccg tggaagcctg ggacgaggaa gggaaaaccg tctggggagc gagtggcgag
     1561 ctggtttgca ccaagcccat accctgccag cccacgcact tctggaacga cgagaacggc
     1621 agcaagtaca ggaaggctta cttctccaaa tacccaggtg tctgggcaca cggcgactac
     1681 tgcaggatca accccaagac aggaggtatc gtcatgttgg gccggagtga tggcaccctc
     1741 aaccccaatg gcgtacgctt tggcagctcg gagatctaca acattgtgga agccttcgat
     1801 gaggtggagg acagcctttg tgtgccccag tacaacaggg atggtgagga gcgggtagtc
     1861 ctgtttctga agatggcctc tgggcacact ttccagcccg acctcgtgaa gcacatccgt
     1921 gatgccatcc gccttggcct gtctgctcgc cacgtgccca gcctcatcct ggagacccaa
     1981 ggcattccat acacaatcaa cggcaagaaa gtggaggtgg ccgtgaagca ggtgatagct
     2041 gggaagactg tggagcaccg gggggccttc tccaaccctg agtccctgga cctgtatcgg
     2101 gacatccctg agctgcagga cttctgaacc aatggctcgc actcggtcca gctgtgcacg
     2161 tgatggaact tagggacagc agctgctccg gtggcccagg cactacgcac tccacgggct
     2221 cagagacagc ttccctgttt cggagtcatg ggtggggacc aggtctgtct cctgagctcc
     2281 aggatgtgcc tggccttcgg agaccaaaag ggtacactga ggcctgtctt ggccctgctg
     2341 ctataggtca gcacacagcc ttgcaggcgc agggctggtg acatttggtg acaacacact
     2401 ggaggagcga tgtggccttc agcctcatga gcccacagtt ccacctcgct ctatgaaggt
     2461 gaaatcttac ctgtattctc tgcccttaat aactggtctg gcttcctcag gtgtactgtc
     2521 ttctcgagag ctgcctgaag ccagggcacc tggtccagca ggcctgtcac ctcacacttg
     2581 gctgttggga tgcctgggct gatggctctg tctgccaagg ccagaacacc attctaggga
     2641 acttcgagtg aatcctccag acagccaaga gctctctgct gtggcaagtc ccctctgctg
     2701 gcgagcgtcc ccgactgttt ctgggtctta acagcacaag tatttgaatg ggtcaaacgt
     2761 caatgttact ggaggatctg ccgtctggag gcacttgaat cccctgggtg tctaccctag
     2821 gttaccactt agctgtgtgc tgggcggtgt gatcctggag tcttcccggg atggagctgc
     2881 ccggcctcag aggaaaatgg ccgcgtgctc cctcctaata gttctgggct ctttctcttg
     2941 tgactgtact ccatgtttgt aaatgacgtc tcaggctttg gctgccagga ctcaatggtg
     3001 cttcttgtct agcacacaga gtatgtcata aatcctggtg attatacttt tgtatttctt
     3061 tctttctttt tttttttttt aaaactgagc acaacataaa accttttaaa aatatcttgg
     3121 attttaactc tataagggaa aagtgctatg aagaatttta tggaataaac ctgtgccatg
     3181 cacgcctatc c
//
