LOCUS       NM_015785               3845 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus zona pellucida binding protein (Zpbp), transcript
            variant 1, mRNA.
ACCESSION   NM_015785
VERSION     NM_015785.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Fujihara,Y., Satouh,Y., Inoue,N., Isotani,A., Ikawa,M. and Okabe,M.
  TITLE     SPACA1-deficient male mice are infertile with abnormally shaped
            sperm heads reminiscent of globozoospermia
  JOURNAL   Development 139 (19), 3583-3589 (2012)
   PUBMED   22949614
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Dun,M.D., Smith,N.D., Baker,M.A., Lin,M., Aitken,R.J. and Nixon,B.
  TITLE     The chaperonin containing TCP1 complex (CCT/TRiC) is involved in
            mediating sperm-oocyte interaction
  JOURNAL   J Biol Chem 286 (42), 36875-36887 (2011)
   PUBMED   21880732
REFERENCE   3  (bases 1 to 3845)
  AUTHORS   Yu,Y., Vanhorne,J. and Oko,R.
  TITLE     The origin and assembly of a zona pellucida binding protein, IAM38,
            during spermiogenesis
  JOURNAL   Microsc Res Tech 72 (8), 558-565 (2009)
   PUBMED   19204925
  REMARK    GeneRIF: The localization of IAM38 on the extracellular side of
            both the inner and outer acrosomal membrane during the cap phase of
            acrosomal development supports the role of this prominent protein
            in acrosomal compaction.
REFERENCE   4  (bases 1 to 3845)
  AUTHORS   Lin,Y.N., Roy,A., Yan,W., Burns,K.H. and Matzuk,M.M.
  TITLE     Loss of zona pellucida binding proteins in the acrosomal matrix
            disrupts acrosome biogenesis and sperm morphogenesis
  JOURNAL   Mol Cell Biol 27 (19), 6794-6805 (2007)
   PUBMED   17664285
  REMARK    GeneRIF: Data show that loss of zona pellucida binding proteins 1
            and 2 in the acrosomal matrix disrupts acrosome biogenesis and
            sperm morphogenesis.
            Erratum:[Mol Cell Biol. 2008 Apr;28(7):2495]
REFERENCE   5  (bases 1 to 3845)
  AUTHORS   Aksu,S., Koczan,D., Renne,U., Thiesen,H.J. and Brockmann,G.A.
  TITLE     Differentially expressed genes in adipose tissues of high body
            weight-selected (obese) and unselected (lean) mouse lines
  JOURNAL   J Appl Genet 48 (2), 133-143 (2007)
   PUBMED   17495347
REFERENCE   6  (bases 1 to 3845)
  AUTHORS   Mori,T., Guo,M.W., Sato,E., Baba,T., Takasaki,S. and Mori,E.
  TITLE     Molecular and immunological approaches to mammalian fertilization
  JOURNAL   J Reprod Immunol 47 (2), 139-158 (2000)
   PUBMED   10924747
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL662805.13, BC139450.1 and AL663071.5.
            
            On Jun 9, 2010 this sequence version replaced NM_015785.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D17569.1, BC139450.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849381 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL662805.13        104021-104051       c
            32-1316             BC139450.1         1-1285
            1317-3845           AL663071.5         125517-128045       c
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 6.76 cM"
     gene            1..3845
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /note="zona pellucida binding protein"
                     /db_xref="GeneID:53604"
                     /db_xref="MGI:MGI:1855701"
     exon            1..356
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..1282
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /note="isoform 1 is encoded by transcript variant 1; zona
                     pellucida-binding protein 1; inner acrosomal membrane
                     IAM38"
                     /codon_start=1
                     /product="zona pellucida-binding protein 1 isoform 1"
                     /protein_id="NP_056600.1"
                     /db_xref="CCDS:CCDS24434.1"
                     /db_xref="GeneID:53604"
                     /db_xref="MGI:MGI:1855701"
                     /translation="MCTQAGSQASTPPAHHPSGSGASVGPSFRPAVCQLPAAGGRAPG
                     NDLLRRGSAVGGVWMEALAPGRAPRGRRRAGASGSVLSPLSLAAVLLCALLRAPPAVG
                     HLARLPRSIHLTQDSLKIVGSTHFPVSVYVMLHQKSPHVLCVTQRLRNTELVDPSFQW
                     HGPKGKLVSENTTAQVTSTGSLIFQSFEETMSGVYTCFLEYKPTVEESIKNLQLKYIV
                     YAYREPRFYYQFTARYHAAPCNSIYNISFEKKLLQILSKLVLDLSCEISLIKSECHRV
                     KMQRAGLQNELFFTFSVASIDTEKGSKPCTDHSCEASKRLSKAKNLIERFFIQQVEVL
                     GKRAEPLPEIYYIEGTLQMVWVNRCFPGYGINVLKHPKCPECCVVCSPGSFNPRDGTH
                     CLQCNNSLVYGAKTCM"
     exon            357..437
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            438..563
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            564..713
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            714..932
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1009
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1187
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..3845
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1375..1380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /note="hexamer: AATAAT"
     polyA_site      1393
                     /gene="Zpbp"
                     /gene_synonym="4930486K01Rik; Iam38; Sp38"
                     /note="major polyA site"
ORIGIN      
        1 tggtgcgggg cctcgaagtc aagggagccc ctaggggatc gacctcttgc ctcccatgtg
       61 tacccaagct gggtcccaag ctagcacacc tcccgcacat cacccgagcg gaagtggcgc
      121 gtccgtcggt ccgtccttcc ggcctgccgt ctgccaactt ccggcggcgg gtgggcgcgc
      181 gccaggtaac gaccttctgc gccgaggctc ggccgtcggc ggcgtgtgga tggaggcctt
      241 ggccccgggc cgggctccgc ggggccggcg gcgggccggg gcctcgggct ccgtgctctc
      301 gccgctgtcg ctggccgccg tcctgctctg cgccttgctg cgggcgcccc ctgcagttgg
      361 acatttggct cgactgccaa gaagtatcca cttgactcaa gattcactga aaatagtggg
      421 atcgacacat tttcctgtga gcgtatatgt tatgcttcat caaaagagtc cacatgtgct
      481 atgtgtaacc caacgactga gaaatacaga gctggtagac ccatcattcc aatggcatgg
      541 gccaaaagga aaacttgttt cagaaaatac cactgcacaa gtgacctcca caggaagcct
      601 catatttcag agctttgaag agaccatgag tggggtttat acatgtttcc tcgagtataa
      661 accaactgta gaggaatcta ttaaaaatct tcaactgaaa tacattgttt atgcttatcg
      721 tgaacctcgg ttttattatc agttcacagc tcgataccat gcagctccct gcaacagtat
      781 ctataacatt tcttttgaga agaaacttct tcagatttta agtaagctgg ttcttgacct
      841 ttcctgtgaa atatccttga ttaagtctga atgccatcgt gttaaaatgc aaagagctgg
      901 tttgcaaaat gaattgttct tcacattttc agttgcatct atagacacag aaaaaggatc
      961 caagccatgt acagaccata gttgtgaagc ttccaaaaga ttatctaagg ctaaaaatct
     1021 catagagaga ttcttcattc agcaagtaga agtcctgggc aagcgtgcgg agccactgcc
     1081 tgagatatac tacattgagg gaacccttca gatggtgtgg gtgaaccgct gctttccagg
     1141 gtatggcata aatgtcctga agcatcccaa gtgcccagag tgctgtgtgg tctgcagccc
     1201 tggatccttc aatccccggg atggaactca ttgcctccag tgcaacaaca gcctggtgta
     1261 tggagcgaag acatgcatgt aggccagggc cttgtgggac ccagcagctc attcaacata
     1321 aaagtatatt tttgaagtac taaagtataa taactcacta ttatgccaaa attaaataat
     1381 cagaatttac aaagatacgg tgtcctcatt caatccagta ctgtatgcat catctttctt
     1441 ggttaatgat aatggttagc tgcatctgac accagatgac tcctttgtca ctgcctttta
     1501 aagattgttt caaatgttct gtattgggtg atgttaaaag atttttcagg aattaaaaaa
     1561 atactcttta tttttactat atgtcaccta tattcaggcc atcctatttt ataagagctt
     1621 taatcttaac ttacaaagtt ttagctattt gaacttggcc tttgacagct caaagccagt
     1681 ttcaaattac atgtcctggg tctccttatt gtacttgtta gtggaagacc tagtcctaga
     1741 gaaatggtgg catttaatat cagtgtattt ggcttaaatg atcagttgac agactcttaa
     1801 accagacata gaagcatagg cctgtaatct taataatttg aagctgatgg caggaagatc
     1861 aaaagtccaa ggagagcttg gactatgtga gaacctgccg caaaaatctc ttacaacgtt
     1921 tgagaatgta gctaccattg aatgttctca gtaggctgaa gcagtttgtc tcccaacagt
     1981 gcttctagga caaaggatta ataaggatca cagttttcag tttttgcacc tttaaaatgc
     2041 atgctgtgtg tcagaagtca ctgctgctac cactaatact atcttctttt acatttggat
     2101 gccacattgg aatatgaaga gttgcgtaaa gatttaaata gaggaaaatc cacaaataac
     2161 tttggagtca ttttctaaag ttcttattga tcagccggtt cacatttcct tcatcttctc
     2221 tgtgagacgt tgcctgcttt gcttttgaca tcagccgctc aaggatggaa aaccaaatag
     2281 ttggatctta ctgagatcct tgaagtggca gcatctttaa gtgccaggtt ggctaacaag
     2341 gaagtatact tgttggcttg tagagataaa gctagctaga aaagatccta ttatatcttc
     2401 ctgtgaacaa gtgcagcctc agtattctca tacagaagaa ggcaattggc ctgtggttgt
     2461 tcttggttag ccctggtggt aattgctttc ttcctctggg ctttcatcat tagcaccaat
     2521 tgctgcttta tagctcatag aggtttttag atgtcttctt ttctctggca ggcaacacag
     2581 tgagtattat actgcttggg taaaatatga aatttctcta cataactagc atattcatcc
     2641 tgtaacttct aggaccccag tctcctcctc taaaacttct tgaaactatc ccaatttctt
     2701 aggaattttc ataggaatag caagacagag tagagatatc aaaagaactg ggctctaagg
     2761 atgacactca attatttccc ttgtaaattg tgaaccccgg cctgctctgc acttcccagc
     2821 tctatgacat aggaaattaa ttatatataa gcaaggtaaa ttttctgtta gagataaaac
     2881 aatcctagct gataaagtac taactacagg gagttgtaaa gcaaacactg ggaaaggagg
     2941 ttatcaaaga accagctatc agcagctgta gctttttgta agctcccata ttcgccatca
     3001 tgaatcattt cttttaatat tatgtgcttt cccatatatt gggattgatt caatatattc
     3061 ttccaattgt caacctagga gagcagaggt agagctgtaa tggtggtgat aagtctggag
     3121 aacagtgtct tccggaagcc atagaccatc tttataggag tagacatgca atagaagtgt
     3181 gtctttagaa atacactcta gaattaagtt cccagtttac acaggcagac tgttcaacaa
     3241 aaccttacca atgttagaga tcagagcaag gtaaaattaa gaggtgaggt caagagaagt
     3301 atctttagaa agagaaacaa aagacagaca ttaatgatta ccctttgctg tttcatatag
     3361 tgaaataatc catttgatta tgtgacatca ctgctgtttt agccaaccat gtgtttgtat
     3421 tcagcattca gctatactag cctatttcta gttatagccc tctgcataca ctatagttag
     3481 gtttttatat gaattaaagg tctatgactg acaatatgtc agtatctaaa acaaaagtta
     3541 cttctgttgt aatgtgataa gatggacagt gttaatcagt gtttaaaatg acatcagcat
     3601 gtgccagaag ctgtgagtgg tctctaaaag attgttgtca tatagcagtt tcagggagtt
     3661 cagcttacat caaaataatt tcttcctgta caataccttt tcaactatat gaacagataa
     3721 ttacctaatt taatacctaa tttaagggga aatatctatt tccattctgt attatattta
     3781 cattaaatat gtaatgtatg ttcatttagc agtatggaaa taaagatctt taagtttggt
     3841 tcagt
//
