LOCUS       NM_001360984            2180 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus zinc finger protein 474 (Zfp474), transcript variant
            1, mRNA.
ACCESSION   NM_001360984 XM_006526158
VERSION     NM_001360984.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Stauber,M., Weidemann,M., Dittrich-Breiholz,O., Lobschat,K.,
            Alten,L., Mai,M., Beckers,A., Kracht,M. and Gossler,A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev Biol 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   3  (bases 1 to 2180)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: Zfp474 is most abundantly expressed in tissues rich in
            highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161119.3.
            
            On Feb 23, 2018 this sequence version replaced XM_006526158.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY350709.1, AB046287.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               AC161119.3         32755-32925         c
            172-294             AC161119.3         24854-24976         c
            295-421             AC161119.3         23992-24118         c
            422-2180            AC161119.3         9010-10768          c
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 28.24 cM"
     gene            1..2180
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="zinc finger protein 474"
                     /db_xref="GeneID:66758"
                     /db_xref="MGI:MGI:1914008"
     exon            1..171
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /inference="alignment:Splign:2.1.0"
     exon            172..294
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /inference="alignment:Splign:2.1.0"
     exon            295..421
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /inference="alignment:Splign:2.1.0"
     exon            422..2180
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /inference="alignment:Splign:2.1.0"
     CDS             627..1670
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="TSZFP; testis-specific zinc finger protein"
                     /codon_start=1
                     /product="zinc finger protein 474"
                     /protein_id="NP_001347913.1"
                     /db_xref="CCDS:CCDS29248.1"
                     /db_xref="GeneID:66758"
                     /db_xref="MGI:MGI:1914008"
                     /translation="MESGNERISSQSQGTIHLSKEPTFLIQQATLPSDLHSTLLQETQ
                     CGGLTKNIKANTQKRRPGTVILSKRSSRIMSETQPRPPVIPSRRPGFRICYICGREFG
                     SQSLAIHEPQCLEKWRTENSKLPKHLRRPEPSKPQPIGGTDSHSLQAANEEAFQSAQA
                     QLLPCENCGRTFLPDRLLVHQRSCKPKGENPGPPSMGSSNVPTGLKKASSGIPARPRT
                     LICYICGREFGTLSLPIHEPKCLEKWKIENDQLPRELRRPQPQKPQPLPAGQSSQEGA
                     SQAALVPCPNCGRTFAVDRLPVHQRSCKSQPSGPKTSNSNIERKGGPNPPTNSKQQRN
                     MEAPNGDKVTGVI"
     regulatory      2001..2006
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="hexamer: ATTACA"
     polyA_site      2029
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="major polyA site"
     regulatory      2118..2123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="hexamer: AATAGA"
     polyA_site      2137
                     /gene="Zfp474"
                     /gene_synonym="4933409D10Rik; Znf474"
                     /note="major polyA site"
ORIGIN      
        1 aacagaagct ggggttcctt ttacacggca gctatacctg ctaactccaa gtccccgcag
       61 gagaaatgtg aaccttagag ttcccagctc ctgcgccatc ctctgtggct cctcacaggg
      121 agctggaacg ccacagcaaa ggccaagcat ccagaagagg acaaatctaa ggtgactttt
      181 atatccacaa aacaggaaga aacaggtcca gagatgttaa gaacttcact caaaattcca
      241 gctgatagga ctttgagaac tatgctatga accaaagtcc aagtttatgc agagttttct
      301 tttaaaggaa gctgtactat attgctgcct gtgccctgga gtacttgcca cctgacccta
      361 cctgtccttg ggcgttggag gtgagatggc acagagtcaa tgacacagac tccatgagca
      421 gatcttggag acatctaagg tttaatgggg gctttcccac attccggact gctgccctcc
      481 aatgacgctc tgagtcattg atccaagtac tggcaacatg tggaagccca ggacaggtca
      541 gcgtcactag cctccagtct aagcaacagc cgagaaccag tgtggtctga aactccagca
      601 agtactagac aaagcctcct ctgttcatgg aaagcggaaa tgaaaggatt tccagccaat
      661 cacaaggaac tatccacctc tccaaagagc ccaccttcct tatccagcag gctaccctgc
      721 cgagtgacct ccattcaacc cttctgcagg aaacacagtg tggcggtcta accaaaaaca
      781 taaaggccaa cactcagaaa aggcgtccgg gaacagtgat tctatcaaaa cgctcaagtc
      841 gaattatgtc agaaacccag cctagacccc ctgtgatccc atcccgcagg ccagggttcc
      901 ggatatgcta tatttgtggc cgagaattcg ggtcccagtc acttgccatt catgagcccc
      961 agtgcttgga gaagtggcgc actgagaaca gcaaactacc caagcacctg aggaggccag
     1021 aaccctccaa accgcagccc atcggtggca ctgactccca cagccttcag gcagccaatg
     1081 aggaagcatt tcagagtgct caggctcagc tgctgccctg tgaaaactgc ggccgcacgt
     1141 tcttgccaga ccgtctcctg gttcaccaga gaagctgcaa gccaaagggt gagaaccctg
     1201 gaccaccaag catgggtagt tctaatgttc ctactggtct caagaaagct tctagcggca
     1261 tcccagcccg accaaggact ctcatctgtt acatttgtgg tagggaattt ggcacgctgt
     1321 cccttcctat ccatgagccc aaatgcttgg aaaagtggaa aattgagaat gaccaactcc
     1381 ctagagagct gcgtcggcca cagccccaga agcctcaacc ccttccagct ggacagtcca
     1441 gccaagaggg ggcgagtcaa gccgcacttg tgccttgccc aaattgtggc cggacttttg
     1501 ctgtggaccg cctacctgta caccagagaa gttgtaaatc tcaacctagt ggaccaaaaa
     1561 cttcaaattc gaacatagaa aggaaaggcg gtccaaatcc acccactaat tccaagcaac
     1621 agaggaacat ggaagcaccc aatggggaca aggtaactgg tgtcatttaa gatgaagtag
     1681 gtggactggg gacacactac atcttcaaaa ggatgagaga actattccta gagtcagcca
     1741 cctcagcccc aacgatggtt tctaccaaag ccttacttgt gtctcaaagc agcctgtcca
     1801 agtggctctc cttttggact ccaggggctg agtgtgtgtc tatttttgca aagtagacct
     1861 aacagaatcc ttgtctggct tgttcataat cctcttcagt cctacagcct aaaagaaatg
     1921 gactgttttc tctgatccct cgtcccagta atccctggga gagactgtta cttaaaatga
     1981 gtcattaatg ctgtgtctac attacagaga tatgattccc aacccaacag ccaatgttta
     2041 ttgctggttt attttagtca tctaccttag gaattcggtt ttggcaatgt tgggttatgc
     2101 tgagtttatc ttactaaaat agaatctgtg tcaaaaaccc ggaatgactt tagcagtaat
     2161 taaacactgg aatacttgct
//
