LOCUS       NM_001358840            4645 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus zinc finger protein 300 (Zfp300), transcript variant
            1, mRNA.
ACCESSION   NM_001358840 XM_006527627
VERSION     NM_001358840.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Wang,J., Wang,J. and Tian,C.Y.
  TITLE     Evolution of KRAB-containing zinc finger proteins and their roles
            in species evolution
  JOURNAL   Yi Chuan 38 (11), 971-978 (2016)
   PUBMED   27867147
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL671853.7.
            
            On Dec 12, 2017 this sequence version replaced XM_006527627.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10662773.1583754.1,
                                           SRR7974084.28465.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               AL671853.7         177791-178014       c
            225-283             AL671853.7         177307-177365       c
            284-410             AL671853.7         173001-173127       c
            411-503             AL671853.7         172693-172785       c
            504-4645            AL671853.7         167935-172076       c
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 16.59 cM"
     gene            1..4645
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /note="zinc finger protein 300"
                     /db_xref="GeneID:245368"
                     /db_xref="MGI:MGI:3045326"
     exon            1..224
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            225..283
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..2209
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /note="isoform 1 is encoded by transcript variant 1; Novel
                     KRAB box containing protein"
                     /codon_start=1
                     /product="zinc finger protein 300 isoform 1"
                     /protein_id="NP_001345769.1"
                     /db_xref="GeneID:245368"
                     /db_xref="MGI:MGI:3045326"
                     /translation="MTESQESVSFEDVSVDFTQEEWNQLDPIQRSLYRDVMLENYSHL
                     VSLGYPITKPEVIASLEKGVLGIIKREIPSSSCTEDGQAEYNLAWHQKNQNMHLEQAI
                     SIIEKNVTEERSYEYDGFVSPLSQSTWVVSSGPTPYMNNSFGESIKNNLGLVNPSSMS
                     FAAQECYECNRCRKLLIPGRHEISDRMKSQEHNMFGKEHIYNFVQHDLTQLGDKTYEC
                     PECRITLSPSSFLVVHQITHIGEKPYEWARCREAFNNTAHLSLHQTMHTGDKLFECNE
                     CGKSFREESTLHIHQRTHTGEKPYICTECGNAFQEKTNLIIHRRTHKGEKPYKCTDCE
                     KTFNQKAHLNVHQRTHTGEKPFECTNCGKYFREKSTLNIHQRTHTGEKPYVCNECGKA
                     FREKTKLIIHQRTHTGEKPYECSECGRAFNQKAHLSVHQRTHTGEKPFECNECGKAFR
                     EKSTLRRHQRIHTGEKPYVCSECGRAFTLKLSLSAHQRIHTGEKTDGCTVCGKVFSRK
                     SYLMLHQRAHTKERLEKSYECNECDKAFLQKSYLIIHQRVHAGEKPYECNICEKSFSQ
                     KSYLIIHQRIHTGEKPYQCDKCSRAFREKSKLTVHQRTHIGEKPYDCPELERNFPEIK
                     VPHGLENSEMKENKELKMENLS"
     exon            284..410
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            411..503
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            504..4645
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3518..3523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3541
                     /gene="Zfp300"
                     /gene_synonym="D930016N04Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtaactaagg cggggaaaag gactaaaaag tggttatctc aggaagttat gactgatacg
       61 ggaaatataa aagagacttt gccggcataa gaattcagat tttaagatcg gagaagagag
      121 tgaaggagtg cagagttcat tcagagcgga agtgagcagg ctggcactct gcaggcggag
      181 tgagacagac aagtgcaaac acactgcaaa cacgaattta ctcgttctgc ctttttaggc
      241 ctttggcttc tctccacaac agtcagtcat gactgagtcc caggaatcag tgtcatttga
      301 ggatgtgtct gtggacttca ctcaggaaga atggaatcaa ctggacccta ttcagaggag
      361 cctatacaga gatgtgatgc tagagaacta tagtcatctt gtctctctgg ggtatcctat
      421 taccaaacca gaagtgatcg cctctttaga gaaaggagtc cttgggataa taaagagaga
      481 aattccaagt tcaagttgca cagaagatgg tcaagctgaa tacaacttgg catggcatca
      541 gaaaaatcaa aatatgcacc tggagcaagc tatatccatt attgagaaaa atgtaactga
      601 agaaagaagt tatgaatatg atggatttgt aagcccactt tctcagagca catgggtagt
      661 ttcttcagga cccacaccct atatgaataa ctcatttgga gaaagtatta aaaataattt
      721 aggtttagtc aatcctagta gtatgagctt tgcagctcag gaatgttatg aatgtaatag
      781 atgtaggaaa ttattaattc ctggcagaca tgagataagt gacagaatga aatcccagga
      841 gcataatatg tttggaaaag agcatattta taactttgtt cagcatgact tgactcaact
      901 tggagacaaa acctatgaat gtcctgagtg tagaataacc cttagcccaa gttcattcct
      961 tgttgttcat cagatcactc acataggaga aaagccttat gaatgggcaa gatgcagaga
     1021 agcattcaac aacacagcac acctcagtct acatcaaaca atgcacacag gagacaagct
     1081 ttttgaatgt aatgaatgtg gcaagtcctt cagagaggag tcaacactgc atatacatca
     1141 aagaacacat acaggtgaaa aaccatatat atgtactgag tgtggcaatg cttttcaaga
     1201 aaagacaaat cttatcatac atcggagaac tcacaaagga gagaagccct ataaatgtac
     1261 agattgtgag aaaaccttca accaaaaagc tcatcttaat gtacatcaga gaacacacac
     1321 aggagaaaaa ccttttgaat gtaccaattg tggcaagtac ttcagagaga aatcaactct
     1381 gaatatacat caaagaactc atacaggaga gaaaccatat gtatgcaatg agtgtggcaa
     1441 agccttcaga gaaaagacaa aactcattat acatcagaga actcacacag gagaaaaacc
     1501 ctatgaatgt tcagaatgtg ggagagcatt taaccaaaag gcacacctca gtgtacatca
     1561 gagaacacac acaggagaga aaccttttga atgcaatgaa tgtggcaaag ctttcagaga
     1621 aaagtcaaca ctgcgcagac atcaaagaat tcacacagga gaaaaacctt atgtgtgttc
     1681 agaatgtgga agagccttta cccttaagct aagccttagt gctcaccaga gaattcatac
     1741 aggggaaaaa acagatggat gtactgtatg tggaaaagtc ttctcccgga agtcatacct
     1801 catgttacat cagagagctc atacaaaaga aagacttgaa aaatcatatg aatgcaatga
     1861 atgtgataaa gcattcttac agaaatcata tctcattatt catcagaggg ttcatgcagg
     1921 agagaaaccc tatgaatgta atatatgtga gaaatctttc tcccaaaaat catatctcat
     1981 tattcatcaa agaatccata caggagagaa accgtatcaa tgtgataagt gtagcagagc
     2041 cttcagagag aagtccaaac tcactgtgca tcaaagaact cacataggag agaaacccta
     2101 tgactgccct gaattggaaa gaaactttcc agagatcaaa gttcctcatg gattagagaa
     2161 ctcagagatg aaagaaaaca aagaactgaa aatggaaaat ctgtcatagt aaagttaaag
     2221 aaaatggaaa tgcaaaaggc aaggggaaat tgtgctgtag ctagggtgct tttctaatca
     2281 tatgacacac ttgtccttaa agatcagcac cagccatatt gagagaagaa cattctgagt
     2341 aaaagaaata ggaagatctt atttcttaaa gaaaagaact tggcctctgt actttttttt
     2401 atcatggata tattgaatta tcatttgtta tcaaagaggt tgtcaatgct gaaatctttt
     2461 caagtcttca atgtataaaa gctgtcagtt gaaaaacaaa cctcatcata aatgtgggca
     2521 tacatataaa aagaattcaa ttaattaatt tctcagaagt aaagtaaagg aaatatgtaa
     2581 gtttccaaat ttcaaatact catagaaaac gtaaaaataa cgttagaaaa agtggtttac
     2641 caaacaattt taagttaaat atttaaatat aaccattaaa ggaatatatt acaaaattcc
     2701 aaattatttt aataatcaga acaatgtaag aaaatactac tagagtatca aatccagtgt
     2761 taggcagtaa taacagttga taatgtcaat gtttcctccc cagcctgatc tgggaagact
     2821 ataagcttcc aaggactata agatgcctaa taggctttag aagacatgtt tttggcacaa
     2881 attcaccagt gttcatttct gctgctttta attaatgcct accacattca taattttgtc
     2941 aatagccagc atggtagcat cttttatgga ctcccagaga gatgtgaaat ttggttatct
     3001 atcccggttg gggtgaaata atggaaaatt ccagttgacc ttaaggagga ggtcttgaac
     3061 tctccttgcc atgcaaggac agctagctgt tttactgatc gtctgtgttt attttgatga
     3121 acgctgaaag caaggctttg ggtaactgag ccctgaccat ggactacaaa acaacatgag
     3181 caactacttc atgagaatag ctacttccaa tgggactgcc caataaaatg agagcaaatg
     3241 gcaagtagtt aatagctgtg ttcaagacac agcctgaaaa ccaggcattc tgtaaaaaga
     3301 aattatcact ttgtgtgtcc tttgaattct ttgtctaaat tacaagtctg tcattttaaa
     3361 ctttagactt agatcatgaa agagataata ggaggttggg gcttagaatg gaagtatgtt
     3421 agagtatgtg gtagaatgtg actattttaa actctcattg cacactgtat tttctctatt
     3481 taatttaatt ctccccgaag aggccacaaa tgtcttaaat aaagaccctg tagtgaccta
     3541 atctgaaaat gatgctttta aagataggac atctttcata tccttatacc caacccctct
     3601 atctctaaat ctgtaattga attttttttt ttggatgcag ggtctttatg taggctaggc
     3661 tgctttatgg ttgtaatttt atgccattgc cacctaagtg ctggtatgac aggcatgtac
     3721 caccatatac atatcttttt tcaatttttt attagatatt ttcttcattt acatttcaaa
     3781 agctattctg aaagtcccct ataccctccc caaccctgct ccgctaccca cccactccca
     3841 cttcttggcc ctggcattcc cctgtactgg ggcatataaa gtttgcaaga ccaaggggcc
     3901 cctctttcca ttgatggctg actaggccat cttctgctac atatgctgct agagacacaa
     3961 gccctgggga tactggttag ttcatattat tgttccatct ataaggttgc agagtccttc
     4021 agctccttgg gtgctttctc tacctcctcc attgggggcc ctgtgttcaa tccaataaat
     4081 gactattagc atccacttcg gtatttgcca ggcactggca tagcctcaca agagatagct
     4141 atatcagggt cctttcagca aaatcatgtt ggcatatgca atagtgtctg ggtttggtgg
     4201 ttgtgtacgg gatggattcc tgggtggggc agtctatgga tggtctttcc ttccatctta
     4261 gctctgaact ttgtctctgt aactccttcc attgggtatt ttgttcccca ttctaagaag
     4321 aagcaaagta atccacactt tggtctacct tcttcttgag tttcatgtgt tttgcaaatt
     4381 gtatcttgga tattctaaga ttctgggcta atatccactt atcagtgagt gcatttcatg
     4441 tgtgttcatt tgtgattggg ttacctcact caggatgata tcctacagat cgatccattt
     4501 gcctaggaat ttgatgaatt cattgttttt aattgctgag tagtacccca ttgtgtaaat
     4561 gtaccaccat ttctgtatgc attcctctgt ccagggacat ctgggttctt tccaacttct
     4621 ggctattata aataaggctg ctatg
//
