LOCUS       NM_001145888            4501 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus zinc finger and AT hook domain containing (Zfat),
            transcript variant 1, mRNA.
ACCESSION   NM_001145888
VERSION     NM_001145888.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Ishikura,S., Yoshida,K., Hashimoto,S., Nakabayashi,K., Tsunoda,T.
            and Shirasawa,S.
  TITLE     CENP-B promotes the centromeric localization of ZFAT to control
            transcription of noncoding RNA
  JOURNAL   J Biol Chem 297 (4), 101213 (2021)
   PUBMED   34547289
  REMARK    GeneRIF: CENP-B promotes the centromeric localization of ZFAT to
            control transcription of noncoding RNA.
REFERENCE   3  (bases 1 to 4501)
  AUTHORS   Ishikura,S., Nakabayashi,K., Nagai,M., Tsunoda,T. and Shirasawa,S.
  TITLE     ZFAT binds to centromeres to control noncoding RNA transcription
            through the KAT2B-H4K8ac-BRD4 axis
  JOURNAL   Nucleic Acids Res 48 (19), 10848-10866 (2020)
   PUBMED   32997115
  REMARK    GeneRIF: ZFAT binds to centromeres to control noncoding RNA
            transcription through the KAT2B-H4K8ac-BRD4 axis.
REFERENCE   4  (bases 1 to 4501)
  AUTHORS   Doi,K., Tsunoda,T., Koyanagi,M., Tanaka,Y., Yamano,S., Fujikane,A.,
            Nishi,K., Ishikura,S. and Shirasawa,S.
  TITLE     Zfat Is Indispensable for the Development of Erythroid Cells in the
            Fetal Liver
  JOURNAL   Anticancer Res 39 (8), 4495-4502 (2019)
   PUBMED   31366551
  REMARK    GeneRIF: Zfat knockdown partially inhibited CD71(-/low)Ter119(-) to
            CD71(high)Ter119(-) transition of fetal liver erythroid progenitors
            with impairment in the elevation of CD71 expression.  Zfat plays a
            critical role for erythropoiesis in the fetal liver
REFERENCE   5  (bases 1 to 4501)
  AUTHORS   Tsunoda,T., Doi,K., Ishikura,S., Luo,H., Nishi,K., Matsuzaki,H.,
            Koyanagi,M., Tanaka,Y., Okamura,T. and Shirasawa,S.
  TITLE     Zfat expression in ZsGreen reporter gene knock-in mice:
            Implications for a novel function of Zfat in definitive
            erythropoiesis
  JOURNAL   Int J Mol Med 42 (5), 2595-2603 (2018)
   PUBMED   30106088
REFERENCE   6  (bases 1 to 4501)
  AUTHORS   Doi,K., Fujimoto,T., Koyanagi,M., Tsunoda,T., Tanaka,Y.,
            Yoshida,Y., Takashima,Y., Kuroki,M., Sasazuki,T. and Shirasawa,S.
  TITLE     ZFAT is a critical molecule for cell survival in mouse embryonic
            fibroblasts
  JOURNAL   Cell Mol Biol Lett 16 (1), 89-100 (2011)
   PUBMED   21225468
  REMARK    GeneRIF: ZFAT is a critical molecule for cell survival in MEFs and
            MOLT-4 cells at least in part through the regulation of the
            apoptosis involved in the BCL-2- and IL6st-mediated pathways.
REFERENCE   7  (bases 1 to 4501)
  AUTHORS   Tsunoda,T., Takashima,Y., Tanaka,Y., Fujimoto,T., Doi,K.,
            Hirose,Y., Koyanagi,M., Yoshida,Y., Okamura,T., Kuroki,M.,
            Sasazuki,T. and Shirasawa,S.
  TITLE     Immune-related zinc finger gene ZFAT is an essential
            transcriptional regulator for hematopoietic differentiation in
            blood islands
  JOURNAL   Proc Natl Acad Sci U S A 107 (32), 14199-14204 (2010)
   PUBMED   20660741
  REMARK    GeneRIF: ZFAT is indispensable for mouse embryonic development and
            functions as a critical transcription factor for primitive
            hematopoiesis through direct-regulation of Tal1, Lmo2, and Gata1
REFERENCE   8  (bases 1 to 4501)
  AUTHORS   Koyanagi,M., Nakabayashi,K., Fujimoto,T., Gu,N., Baba,I.,
            Takashima,Y., Doi,K., Harada,H., Kato,N., Sasazuki,T. and
            Shirasawa,S.
  TITLE     ZFAT expression in B and T lymphocytes and identification of
            ZFAT-regulated genes
  JOURNAL   Genomics 91 (5), 451-457 (2008)
   PUBMED   18329245
  REMARK    GeneRIF: Study shows that ZFAT protein is expressed in B and T
            lymphocytes in mouse and that ZFAT regulates immune-related genes
            functionally important for immune responsiveness.
REFERENCE   9  (bases 1 to 4501)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 4501)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC108821.6, AC126249.4 and AC140212.2.
            
            On Mar 25, 2022 this sequence version replaced NM_001145888.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               AC108821.6         147628-147825
            199-375             AC126249.4         2421-2597
            376-627             AC126249.4         14520-14771
            628-813             AC126249.4         40128-40313
            814-964             AC126249.4         42793-42943
            965-2406            AC126249.4         46206-47647
            2407-2639           AC126249.4         48421-48653
            2640-2727           AC126249.4         57141-57228
            2728-2877           AC126249.4         58500-58649
            2878-3051           AC126249.4         61438-61611
            3052-3140           AC126249.4         80773-80861
            3141-3279           AC126249.4         108290-108428
            3280-3398           AC126249.4         116802-116920
            3399-3522           AC126249.4         122285-122408
            3523-3653           AC126249.4         125763-125893
            3654-4501           AC140212.2         9322-10169
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 29.44 cM"
     gene            1..4501
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /note="zinc finger and AT hook domain containing"
                     /db_xref="GeneID:380993"
                     /db_xref="MGI:MGI:2681865"
     exon            1..198
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..3893
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger protein 403; zinc finger protein 406; zinc finger
                     protein ZFAT; ZFAT zinc finger 1"
                     /codon_start=1
                     /product="zinc finger protein ZFAT isoform 1"
                     /protein_id="NP_001139360.1"
                     /db_xref="CCDS:CCDS49623.1"
                     /db_xref="GeneID:380993"
                     /db_xref="MGI:MGI:2681865"
                     /translation="METRTAENTAIFMCKCCNLFSPNQSELVTHVSEKHGEEGVNVDD
                     VIIPLRPLNTPENPNPSKGGDEFLVMKRKRGRPKGSTKKPSTEEEVVENLVSPSEDGP
                     LATEEGSRLAPSSLECSKCCRKFSNTRQLRKHICIIVLNLGEEDGDAGNESDLDLEKT
                     YKEDDREKASKRPRAQKTEKVQKISGKEAGQLSGAKKPIISVVLTAHEAIPGATKIIP
                     VEAGPPETGAPPPETTAADLVPRRGYQEYAIQQTPYEQPMKSSRLGPTQLKIFTCEYC
                     NKVFKFKHSLQAHLRIHTNEKPYKCSQCSYASAIKANLNVHLRKHTGEKFACDYCSFT
                     CLSKGHLKVHIERVHKKIKQHCRFCKKKYSDVKNLIKHIRDMHDPQDKKVKEALDELR
                     LMTREGKRQLLYDCHICERKFKNELDRDRHMLVHGDKWPFACELCGHGATKYQALELH
                     VRKHPFVYVCALCLKKFVSSIRLRSHIREVHGAAQETLVFTSSINQSFCLLEPGGDIQ
                     QEALGDQLQLAAEEFVCPEIDVRKEEACPGEAQPEVGLRELVVPGDAHAPPPGPLATP
                     QSESSSLSPCKLETTVVSSDLNSLGVVSDDFLLKSDTSSAEPPAAAEATSDTQHRDSA
                     QTQGEEVTLLLAKAKSAGPDPESSPGGRQKVGALPASESDSSTCLRANPTEASDLLPT
                     VTDGGDLGVCQPDSCTPSSEHHPGSTAFMKVLDSLQKKQMNTSLCERIRKVYGDLECE
                     YCGKLFWYQVHFDMHVRTHTREHLYYCSQCHYSSITKNCLKRHVIQKHSNILLKCPTD
                     GCDYSTPDKYKLQAHLKVHTELDKRSYSCPVCEKSFSEDRLIKSHIKTNHPEVSMNTI
                     SEVLGRRVQLKGLIGKRAMKCPYCDFYFMKNGSDLQRHIWAHEGVKPFKCSLCEYATR
                     SKSNLKAHMNRHSTEKTHLCDMCGKKFKSKGTLKSHKLLHTSDGKQFKCTVCDYTAAQ
                     KPQLLRHMEQHASFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYTNVGSGELAAEAL
                     IQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWEPAAETPEEPSTQYLY
                     ITEAEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGSETHDAAAVASVV
                     AMAPGTVTVVKQVTDEEPSSNHTVMIQETLQQASVELAEQHHLVVSSDDVEGIETVTV
                     YTQGGEASEFIVYVQEAVQPVEEQVGEQPAPEL"
     exon            199..375
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            376..627
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            628..813
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            814..964
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            965..2406
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2639
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            2640..2727
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2877
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..3051
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..3140
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3279
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3280..3398
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3522
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3653
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     exon            3654..4501
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4501
                     /gene="Zfat"
                     /gene_synonym="Gm922; Zfat1; Zfp406"
                     /note="major polyA site"
ORIGIN      
        1 gaagactcca tccgccatgt tggatgccgc agattcgcca taacctctcc ggctctttcc
       61 ctaaaaaaat caaaataaag agcgaagttt cctcagggtg tccgactttc tcgctcagcc
      121 cgggagggag ccgggaagag cccgaggctt tttttttcct ccgccgtggg ggcgttgcca
      181 tggagacgcg gacggcagaa aacacagcca tcttcatgtg taaatgctgt aatcttttct
      241 caccaaatca gtcagaactc gtgacccacg tttctgagaa gcatggggaa gaaggggtca
      301 atgtggatga cgtcatcatt cccctccggc ctctgaacac tccagaaaac cccaacccaa
      361 gcaagggtgg agatgagttt ttggttatga agagaaagag agggaggcct aagggttcca
      421 caaagaagcc cagcactgaa gaggaggtgg tagaaaacct cgtgagcccc agtgaggacg
      481 gtcccctggc tacagaggaa gggagcagac tggcccccag cagcttggag tgtagcaagt
      541 gctgccggaa gttctccaac acacgccagc tgcgcaagca catctgcatc attgtgctga
      601 atctgggtga ggaggatgga gatgcaggta acgagtcaga ccttgatcta gaaaagacat
      661 acaaagaaga tgatcgagaa aaggcttcaa aaagaccccg ggctcagaaa acagagaaag
      721 tccagaagat ctcaggaaag gaggcagggc agctttctgg agcgaagaaa cccatcatca
      781 gcgtggtctt aactgctcat gaagcaattc caggtgctac caagattatc ccagtggagg
      841 ctgggcctcc cgaaacagga gcaccccctc cagagaccac tgcagcagac ctggtgccgc
      901 ggagagggta ccaagaatac gccatccagc agaccccata tgagcagccc atgaagtcaa
      961 gcaggttagg tcccacccag ctgaagatct tcacttgtga gtactgcaac aaggtgttca
     1021 agttcaagca ctctctgcag gcccacctga ggatccacac gaacgagaag ccatacaagt
     1081 gctcccagtg cagctatgcc agcgccatca aggccaacct caacgttcac ctgcgcaagc
     1141 acaccggaga gaaattcgcc tgcgactact gctccttcac ctgcctcagc aagggccacc
     1201 tcaaggtgca catcgagcgg gtacacaaga agatcaaaca gcattgccgc ttctgcaaga
     1261 agaaatactc agatgtcaag aacctcatca agcacatccg ggacatgcat gacccgcagg
     1321 acaagaaggt caaggaggcc ttagacgagc tccgcctcat gacaagggag ggcaagcggc
     1381 agctgctcta cgactgccac atctgcgagc gcaagttcaa gaatgagcta gaccgagacc
     1441 gccacatgct ggttcatggt gacaagtggc cctttgcctg cgagctctgt ggccatgggg
     1501 ccactaagta ccaggccctg gaactgcatg tccgaaagca cccctttgtg tatgtctgtg
     1561 ctctatgtct caagaagttc gtcagctcca tcaggctgcg ctcccatatc cgagaggtgc
     1621 atggggcggc ccaggagacc ttggtgttta ccagctccat caaccagagt ttctgcctcc
     1681 tggagccggg cggggacatc cagcaggaag ccttggggga ccagctacag ctggcagctg
     1741 aggaatttgt gtgtccagaa attgatgtac gcaaggagga ggcttgtcct ggggaagctc
     1801 agcctgaggt ggggttgagg gagttggtgg tccctggaga tgcacatgcc cctccacctg
     1861 ggcccttggc caccccccag agtgaaagta gttccctgtc cccctgcaaa ctagaaacca
     1921 ctgtggtctc ctccgacctc aactctcttg gagtggtttc agatgatttt ttactgaaaa
     1981 gcgatacctc ctctgctgag cctcccgctg ctgccgaggc aacctcggat acacagcatc
     2041 gagactcagc ccagactcag ggtgaagaag tcacactgct gctggccaag gccaaaagtg
     2101 ctggaccaga cccagagagc tctccgggtg gaaggcagaa ggtgggtgct ctgccagcca
     2161 gtgaatctga ctctagcaca tgtctcaggg caaacccaac agaagcctca gacctccttc
     2221 ctacagtgac tgatggaggg gaccttggtg tgtgccagcc tgactcctgc acaccatcct
     2281 ctgagcacca ccctggcagc acagcattca tgaaggtcct ggacagtctc cagaagaagc
     2341 agatgaacac cagtctttgt gagcggatcc ggaaggttta cggagacctg gagtgtgaat
     2401 actgcggcaa acttttttgg taccaggtgc attttgacat gcatgtccgc acccacaccc
     2461 gggaacatct gtattattgc tcccagtgtc actattcttc catcaccaaa aactgcctta
     2521 aacgccatgt aattcagaaa cacagtaaca tcttgctgaa gtgccccact gacggctgtg
     2581 actactcgac tccagataaa tataagctgc aggcccatct taaagttcac acagagctgg
     2641 acaaaaggag ttattcttgt cctgtatgtg aaaaatcatt ttcagaagac cgattgataa
     2701 agtcacatat caagactaat catccagagg tctccatgaa taccatttct gaggttcttg
     2761 ggagaagagt ccagctcaaa gggctaattg gaaagcgagc catgaagtgt ccatattgtg
     2821 atttctattt catgaagaat ggctcagacc ttcagcggca catctgggct catgagggtg
     2881 tgaagccctt caagtgttct ctgtgtgaat atgcaactcg tagcaagagc aacctcaaag
     2941 ctcatatgaa tcgtcacagc actgagaaga cccacctctg tgacatgtgt ggcaagaaat
     3001 tcaaatccaa agggacactg aaaagtcata agctccttca cacgtctgat gggaagcaat
     3061 tcaagtgcac ggtgtgtgac tacacagcag cccagaaacc ccagctgctg cgacacatgg
     3121 agcagcatgc ctccttcaag cctttccgct gtgctcactg tcattactct tgtaacatct
     3181 ctggatctct gaaaagacac tacaacagga agcaccccaa tgaggagtac accaacgtgg
     3241 gcagcgggga gctcgcagct gaagccctca tccagcaagg tggtctgaag tgtcctgttt
     3301 gcagctttgt gtatggaacc aaatgggagt tcaacagaca cttgaagaac aaacatggct
     3361 tgaaggtagt ggagattgat ggagacccca aatgggagcc agcagcagag actcccgagg
     3421 agccctccac ccagtatctc tacatcactg aagctgaaga tgttcagggg acacaagcag
     3481 ctgtggctgc acttcaggac ctgagatata cctccgagag tggtgatcga cttgacccca
     3541 ccgctgtgaa tatcctgcag cagatcattg aactaggttc ggagacccac gatgctgctg
     3601 ctgtggcctc tgtggttgcc atggcgcccg gcacagtgac tgttgtaaag caggtcaccg
     3661 atgaggagcc cagttccaac cacacagtca tgattcagga gactctccag caggcctccg
     3721 tggagctggc cgagcagcac catctggtgg tgtcctctga cgacgtggag ggcatagaga
     3781 cagtgacggt gtacacacag ggtggggagg cctcagagtt catcgtatac gtgcaagagg
     3841 ctgtacagcc cgtggaggag caggttgggg agcagccagc cccagaactc tagaatccct
     3901 gcctcctgtg gcagccagcc ttggtggccc tggagaccac ctaacccacc agacccatct
     3961 gtgctcttct tgtctactgg cccagataga gttctctgta gtgtcctttg tgtggtcaaa
     4021 gccagcacac caccagcaat acagaatcct agtcctcagt acaaatcaca tcctgaccca
     4081 ggaccctctg tttccaggag accttcccat cagttctcct gtcctacgcc ttccttgctt
     4141 caagatccgg agactcctct ccccatctgc atcatctctg attataaccc cagccctttg
     4201 catccctact acctggtgct caccttctgc aggggcccac tgtgctgtct gtgagaggac
     4261 gacagatatc acacagcaag tggccttatg tcttttgagt taaccactgg gtagggtggg
     4321 gcagggcctt ccaagcattc tccacagagc cctgccctcc tcagtgcaag gacactggga
     4381 acaaatgagt gacccatagt cctctatcac aagggcccag cttggaattc tttttgcttt
     4441 tgaaataaat gcattttctc accccccccc ccatttaggg ggggtgggtg aaataaaagc
     4501 a
//
