LOCUS       NM_028673              12621 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus zinc finger, DBF-type containing 2 (Zdbf2), transcript
            variant 1, mRNA.
ACCESSION   NM_028673 XM_908801 XM_991644
VERSION     NM_028673.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Glaser,J., Iranzo,J., Borensztein,M., Marinucci,M., Gualtieri,A.,
            Jouhanneau,C., Teissandier,A., Gaston-Massuet,C. and Bourc'his,D.
  TITLE     The imprinted Zdbf2 gene finely tunes control of feeding and growth
            in neonates
  JOURNAL   Elife 11, e65641 (2022)
   PUBMED   35049495
  REMARK    GeneRIF: The imprinted Zdbf2 gene finely tunes control of feeding
            and growth in neonates.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Jiang,W., Shi,J., Zhao,J., Wang,Q., Cong,D., Chen,F., Zhang,Y.,
            Liu,Y., Zhao,J., Chen,Q., Gu,L., Zhou,W., Wang,C., Fang,Z.,
            Geng,S., Xie,W., Chen,L.N., Yang,Y., Bai,Y., Lin,H. and Li,X.
  TITLE     ZFP57 dictates allelic expression switch of target imprinted genes
  JOURNAL   Proc Natl Acad Sci U S A 118 (5) (2021)
   PUBMED   33500348
REFERENCE   3  (bases 1 to 12621)
  AUTHORS   Greenberg,M.V., Glaser,J., Borsos,M., Marjou,F.E., Walter,M.,
            Teissandier,A. and Bourc'his,D.
  TITLE     Transient transcription in the early embryo sets an epigenetic
            state that programs postnatal growth
  JOURNAL   Nat Genet 49 (1), 110-118 (2017)
   PUBMED   27841881
REFERENCE   4  (bases 1 to 12621)
  AUTHORS   Duffie,R., Ajjan,S., Greenberg,M.V., Zamudio,N., Escamilla del
            Arenal,M., Iranzo,J., Okamoto,I., Barbaux,S., Fauque,P. and
            Bourc'his,D.
  TITLE     The Gpr1/Zdbf2 locus provides new paradigms for transient and
            dynamic genomic imprinting in mammals
  JOURNAL   Genes Dev 28 (5), 463-478 (2014)
   PUBMED   24589776
  REMARK    GeneRIF: a maternal germline differentially methylated region (DMR)
            at the mouse Gpr1/Zdbf2 (DBF-type zinc finger-containing protein 2)
            locus, which controls the paternal-specific expression of long
            isoforms of Zdbf2 (Liz) in the early embryo
REFERENCE   5  (bases 1 to 12621)
  AUTHORS   Kobayashi,H., Sakurai,T., Sato,S., Nakabayashi,K., Hata,K. and
            Kono,T.
  TITLE     Imprinted DNA methylation reprogramming during early mouse
            embryogenesis at the Gpr1-Zdbf2 locus is linked to long
            cis-intergenic transcription
  JOURNAL   FEBS Lett 586 (6), 827-833 (2012)
   PUBMED   22449967
  REMARK    GeneRIF: Results identified a novel, paternally expressed, long
            intergenic non-coding Zdbf2 variant (Zdbf2linc) transcribed from
            maternally methylated Gpr1 during early embryogenesis in the mouse.
REFERENCE   6  (bases 1 to 12621)
  AUTHORS   Hiura,H., Sugawara,A., Ogawa,H., John,R.M., Miyauchi,N.,
            Miyanari,Y., Horiike,T., Li,Y., Yaegashi,N., Sasaki,H., Kono,T. and
            Arima,T.
  TITLE     A tripartite paternally methylated region within the Gpr1-Zdbf2
            imprinted domain on mouse chromosome 1 identified by meDIP-on-chip
  JOURNAL   Nucleic Acids Res 38 (15), 4929-4945 (2010)
   PUBMED   20385583
  REMARK    GeneRIF: Identification of differentially methylated regions within
            the Gpr1-Zdbf2 imprinted domain.
REFERENCE   7  (bases 1 to 12621)
  AUTHORS   Guttman,M., Garber,M., Levin,J.Z., Donaghey,J., Robinson,J.,
            Adiconis,X., Fan,L., Koziol,M.J., Gnirke,A., Nusbaum,C., Rinn,J.L.,
            Lander,E.S. and Regev,A.
  TITLE     Ab initio reconstruction of cell type-specific transcriptomes in
            mouse reveals the conserved multi-exonic structure of lincRNAs
  JOURNAL   Nat Biotechnol 28 (5), 503-510 (2010)
   PUBMED   20436462
  REMARK    Erratum:[Nat Biotechnol. 2010 Jul;28(7):756]
REFERENCE   8  (bases 1 to 12621)
  AUTHORS   Kobayashi,H., Yamada,K., Morita,S., Hiura,H., Fukuda,A., Kagami,M.,
            Ogata,T., Hata,K., Sotomaru,Y. and Kono,T.
  TITLE     Identification of the mouse paternally expressed imprinted gene
            Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on
            chromosome 2
  JOURNAL   Genomics 93 (5), 461-472 (2009)
   PUBMED   19200453
  REMARK    GeneRIF: Zdbf2 was paternally expressed in various embryonic and
            adult tissues, except for the placenta and adult testis, which
            showed biallelic expression of the gene.
REFERENCE   9  (bases 1 to 12621)
  AUTHORS   Thuret,S., Bhatt,L., O'Leary,D.D. and Simon,H.H.
  TITLE     Identification and developmental analysis of genes expressed by
            dopaminergic neurons of the substantia nigra pars compacta
  JOURNAL   Mol Cell Neurosci 25 (3), 394-405 (2004)
   PUBMED   15033168
REFERENCE   10 (bases 1 to 12621)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK015271.1 and AL669947.14.
            
            On or before Aug 16, 2009 this sequence version replaced
            XM_908801.3, XM_991644.2.
            
            Transcript Variant: This variant (1) encodes the shorter isoform
            (1). Variants 1 and 2 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK015271.1, SRR7345562.741327.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 19200453, 22449967
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-983               AK015271.1         2-984
            984-12621           AL669947.14        61398-73035
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1"
     gene            1..12621
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /note="zinc finger, DBF-type containing 2"
                     /db_xref="GeneID:73884"
                     /db_xref="MGI:MGI:1921134"
     exon            1..204
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            205..287
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            288..340
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            341..451
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            452..554
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             510..7991
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DBF4-type zinc finger-containing protein 2 homolog"
                     /codon_start=1
                     /product="DBF4-type zinc finger-containing protein 2
                     homolog isoform 1"
                     /protein_id="NP_082949.1"
                     /db_xref="CCDS:CCDS48278.1"
                     /db_xref="GeneID:73884"
                     /db_xref="MGI:MGI:1921134"
                     /translation="MEAQSYQEVMKNNGQHLFSSQHRSLTRQSRRRTATNNTLMERFL
                     QDVLRHHPYNYQDNRSAPNEPEAAAAAAAAADPGSPEVVVVLDDSDEKEDDTADSGAE
                     RNSEDSGSVEEIDYRPGTSQEHAEVAVRPSVIQKLERGQQQSLELAHKVESGVKKVNS
                     VGVVQATTSGKKLVRPPVICNAPASSLPSGSFERPVAANSVPRLVLAVASDSFPACDT
                     ENLETYFDSPDQGPSNPSSQPKTKDPKKKLSINLDKLLAQRNLRAKGASFSPVVRVRE
                     LTGSELCSVRAESSELEAGTAGNPRETDTLPEQAREGAIPKRREASRSNTVRPQEETR
                     LVLNKPTLLKQKRSVSSEERFSCGSRQAVPGPSQAAVRDLSLLEEEVEEEKQEEEEEE
                     EQEEEEEHEEEEEGVDQEDASYESRGSDMSFDCGSSCQSLSALSELTAREINVSEETH
                     AYSQPRNETPTVSGATSDNGSSSRQVITQNIQLISLVDESYESSGSEVNFDGDDSLPS
                     TSHRPPQPVEVVVPLRLVDKSYGSGSSEPCEDSGSSADETPAASRQQNPVRNTHANLV
                     DENYGSSSFSSDSDAALDHPQVPVQEGSPRGRAVGQGNEEQPSSAEAHPERDGSLETV
                     AHELQRESQEINLPNQKNTSLGDMNCESHGPEVGFHADAQLEADQSPVNPEEVDLDLE
                     NQSVHSGISNLSFDSNASYQSANDQPQGAWGEVNLDELNVDMEVKSNGCSSSELTFDS
                     DSPLLSVTERSLLDFEGLNEDDFNLEDENCVSSSSDITFDSDIPDDSVADQPQVAVYE
                     EEPVGLENKSNESCVSGITFDSDIPLHSGNDHPEVAVKEVIIKEDENVHLEGKNDNPS
                     GSEICLDSNVPLHSVTNSDVAVKKINPPKEDQVQIEQIEQKENEPTDSELNLDCNSVN
                     SKPGCSEDPIILRVSETRLDSHVPFQSVIRKCEVVVKNVCLQKEKHAELTSKSTESHS
                     EVSSASTASHPVTEPYVGKKAKRKTKHLEEVNSDDEYGGSQPTFKFDVFPRTMTEKPQ
                     PAALKEGHADPKDKITELRGMAVNVNTADCLDSVLSQPQLASNENCVELKDTDGKPSD
                     SKASADSTGHFHSLPKQEFDVVSKMNEWKKEAKVLEQKISDLIYSKIIHDSNVSFRSA
                     MDQLELALKQISLGNNDQVSLEDNSQDTCSETNLDSGFSVQAVVEPPEPEVTVLEPEH
                     VEQEGRNNVPCDSEVSVDANGSVQLEAGQHSESGENRSQKDTDDTEGKRDDAQGFGIT
                     CDSNVPQPLAGHIEVVQDIDHWKDHVDLEDKLGESKNSKVNFHSDEPLQAVTNATQEP
                     VEEINLPRGRASPNGNGCEPYGSTIVPVTNVIFCSVIQKPQRLQKKCTSLKENSSNPC
                     PEVNVDSCDGPEVSVDSNDPCQSVAGHLQKPDKEMNLKEDHIYLEDKSYKLVDFEPTY
                     DSDDPVQFVTVPSVEAVSIKEVNLQKENPDDLENENFQPCCSEVACNSAVHLQSEADP
                     PQVACKEADLDKKALDIEDKGSVTCVPEVVYDSDVSFQIVVNQVQTSDGETDSPQVVF
                     VDVVSSDSDCDREVISDSNIPLQLEPPQMTVKETSDINTDSLGSAANEKYYCKFCGCD
                     YEASQSVTNQSKESFKIINRKNDYIILGDSTCPSCGHELNFNVDPSDQPTTCQLQQPD
                     RNFIDPEDKNFGSKCPKRKLNWEDTAHPVTHQLQKTGEATSLRKDQKNRFKRDRGWES
                     SSFAGDHAAYAVSVIRQTALNSCGSELNFQDDTFYHSDIDPPQPKKKGQGKKVTFDLR
                     VTKYEYPPNPMYGEGEEVAEDDLKEVVVHEASPQGQAPPSIVGKTCPQGREDDVKTNT
                     QACQGYFYSYYDGGSETKKILLGEEEKTIRSDLNQNTTSIQHVKGKVGDTGDFSVDLG
                     KQSCSLAEGLHQQHGQVTSQNQVEVRCGIQASSGKKRKITEQEEDSPKRKCFHHDSQK
                     KKKAQAGITELPEPQTKVLEPVQPDSLVYIFSSLSMKEDQSLNPPKTKPGSDSDLPHI
                     YSCREHTSSGPRRKRTVINPPQNLMVPEVGIDLNRHDPKPNAGDDSAKRQNLVSTSFM
                     AMPKKSVLKFQRTNQSSFLKKSEDVGATQVPKDNFQQTLVNRDGAKKFPKSVKKEDLE
                     SQNQRKFWKKKMVAANKLCLIKNAYKTMVLRKKSKLASEKLAIWIQLKATDIIRKYVS
                     RCHGLMPRRHLSKTVLIRMQLRKKKIVARKIKEAKRAAEALALKLSRPSCPPRAPCPP
                     RALCPPRAPCPPRALCPPRAPCPPRALCTPRAPCPPRALCTPRAPCPPRALCTPRAPC
                     PPRALRAPCPPRALCSPRAPCPPRALCPPRAPCPPRAPRAPCPPRAPCPPRAPCLPRA
                     PCPPRAPCPPRAPCLPRAPCLPQAPCPPRALCLPRVSCPPLPLCAPCPPCAPVLPAGA
                     EEQLSTTAGPAELPAHLSNAGGIKRYRKTYFRRRKRLLPVREYDLRSLSSTTNTDRMV
                     TRLASKSKSNEAK"
     exon            555..685
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            686..12621
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10473..10478
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      10496
                     /gene="Zdbf2"
                     /gene_synonym="4930431J08Rik; 9330107J05Rik"
ORIGIN      
        1 agtcgcgact cggagcccgc gtcgagcgac acgcgctccg ctccgctccg ctcctccgtt
       61 ccgggcgggc gtggcccggg gcccggcggc ttcgggacgt ttcgggggtc ctcgcggcgc
      121 ggcggaaccc aagcggcggg cagcgcgccc aggccggccg cggcccgcag cggcccggaa
      181 ggagagcagg aggcatccac ggaggaccca atggaagtgt ccaggatgct tgtcgccatc
      241 gggaaaccag catagcatgg ataacccaaa aggaaaaaaa agcaaagatg cttgtcaaga
      301 ctaatgcagc tgatgagatg cttgctcttt tctgctcaag tttgagtgtt cgaggccagt
      361 agcctcctga ctgattgact gactaatcaa gatgcagaaa aggcaagggt attgcagcta
      421 ttgccgtgtg cagtatacta acctggaaca gaccctcact aaaggaaaaa ctaaaaggag
      481 ttcttcaggc agaaggaaaa tgattccaga tggaagctca gagctatcag gaagtaatga
      541 agaacaatgg acagcacttg tttagttctc agcacagaag cctgaccaga cagagcagac
      601 gtcggacagc tactaataac actctgatgg aacgcttttt gcaggatgtt ctgcgacacc
      661 acccatataa ttaccaagac aacagatctg caccaaatga gccagaggcg gcggcagcag
      721 cggcagcagc agcagatcct ggatcacctg aagtggtggt ggttttggat gattctgatg
      781 agaaggagga tgatactgca gatagcggtg cagagaggaa ctccgaggat tcaggatctg
      841 ttgaagagat agattataga cctggtactt ctcaggaaca cgcagaagtt gcagttcgac
      901 catcagttat tcaaaaactg gaacggggac agcagcagtc cttggaattg gctcataaag
      961 ttgagagtgg tgtgaaaaaa gttaattcag tcggtgttgt tcaggctaca actagtggaa
     1021 aaaagttagt gcgtccccct gtgatttgta atgctcctgc cagctctttg cctagcggct
     1081 ctttcgagag accagttgcg gctaacagtg ttcctcggtt agtactggca gttgcttcag
     1141 attcttttcc agcttgtgac acagagaacc ttgaaacata ctttgactcc ccagatcagg
     1201 gccctagcaa cccatcatct caacccaaaa ctaaagaccc aaaaaagaaa ctaagtataa
     1261 atttagacaa attgcttgca cagagaaatc ttagggctaa gggtgcgtct ttttcccctg
     1321 tcgtacgagt ccgtgagcta acaggttctg agctttgttc tgtaagagct gagtcttctg
     1381 agttagaggc aggtacagca ggaaacccga gggagactga cacactacca gaacaagcac
     1441 gtgaaggtgc cattccaaag cgtcgtgagg catctcgttc aaatacagtt cgtccccaag
     1501 aagaaacacg tttggttctt aacaagccaa cacttctgaa acagaagcgc tcagtgagtt
     1561 ctgaagagag gttcagttgt gggtctcgtc aggcagtacc tggtccttcc caagctgctg
     1621 tacgtgactt aagtcttttg gaggaggaag tggaggagga gaagcaggaa gaagaggaag
     1681 aggaggagca ggaagaagag gaggagcatg aagaggagga ggagggagtt gaccaagaag
     1741 atgcgagtta tgaatctaga ggttctgaca tgagttttga ttgtgggtcc tcttgtcagt
     1801 cgctgagtgc cctatctgaa ctgaccgcca gagaaataaa tgtatcagaa gaaacacatg
     1861 cttattctca gcctaggaat gaaacaccca ctgtttctgg agcaacttca gataatggta
     1921 gcagttctcg tcaggtgatt acacagaaca tacagctcat tagtctggtt gatgaaagct
     1981 atgagtctag tggctctgaa gtgaattttg atggtgatga ctcactaccg tcaactagtc
     2041 accgtccccc acagcccgtg gaagtagtag tgccccttcg tttggttgac aagagctatg
     2101 gaagtggtag ctctgaacca tgtgaggatt ccgggtcctc agctgatgag actccagcag
     2161 catcaagaca gcagaatcct gtgaggaaca cccatgcaaa cctggttgat gagaactacg
     2221 ggtcaagtag tttcagctct gacagtgatg cagctctgga ccatccccag gtgcctgttc
     2281 aagaaggaag ccccagaggc agagctgtcg gacagggaaa tgaagagcag cccagtagtg
     2341 ctgaagcaca tcctgaacgt gatggttctc ttgagacagt ggctcatgaa ctccagagag
     2401 aatcacaaga aataaacctt ccgaaccaga agaataccag ccttggggat atgaactgtg
     2461 agtctcatgg tcctgaagtg ggttttcatg ctgatgctca attagaggct gatcaatctc
     2521 cagtaaaccc tgaggaagta gatcttgacc tagaaaatca gagtgttcac tctggcattt
     2581 ctaacctaag ttttgattcc aatgcttctt atcagtcagc taatgatcaa cctcaagggg
     2641 cttggggtga agtaaatctc gatgagttaa atgtcgacat ggaagttaag agcaatggct
     2701 gctccagttc tgagttgaca tttgattccg attcccctct tctgtcagtt actgagcggt
     2761 ctctgctgga ttttgaagga ttaaacgaag atgactttaa cctggaagat gaaaactgtg
     2821 tgtcaagtag ttctgacata acttttgatt ctgatattcc cgatgactca gtagctgacc
     2881 aacctcaagt agctgtttat gaggaggaac ctgttggtct ggaaaataag agtaatgaat
     2941 cttgtgtttc tggaataaca tttgattctg atattcctct tcattcagga aatgatcacc
     3001 ctgaagtagc tgttaaagaa gtaatcatta aggaagacga aaacgttcac ttagaaggga
     3061 agaatgacaa tcccagtggt tctgaaatat gtttggattc taatgtccct cttcattcag
     3121 tgactaattc tgatgtagct gttaaaaaga taaatccccc caaagaagat caggtacaaa
     3181 tagaacaaat tgaacaaaag gaaaatgaac ctactgattc tgaattaaat ttggattgta
     3241 actctgttaa ttcaaagccc ggatgttctg aagatcccat tatattacgt gtttctgaaa
     3301 caagattgga ttctcatgtc ccctttcagt cagttattcg caaatgtgaa gtagttgtca
     3361 aaaatgtctg tcttcaaaaa gaaaagcatg ctgagttaac aagtaaaagc acggaatctc
     3421 attctgaagt aagttcagct tctactgctt ctcacccagt gacagaacct tatgtaggta
     3481 aaaaggcaaa gagaaagaca aagcatcttg aggaagtcaa cagtgatgat gaatatggtg
     3541 gctctcaacc aactttcaag tttgatgttt ttcctcggac aatgactgaa aaacctcaac
     3601 cagctgcttt gaaagagggc catgctgacc caaaagataa aattactgag cttagaggga
     3661 tggccgtaaa tgtgaatact gctgattgtc ttgattcagt ccttagccag cctcagttag
     3721 cctcaaacga aaactgtgtt gaactgaaag acaccgatgg caaacccagt gactctaaag
     3781 caagtgctga ttctactggc cattttcact ccctgcctaa acaagagttc gatgtggttt
     3841 caaaaatgaa cgagtggaaa aaagaggcaa aggttcttga acagaagatc agtgatctta
     3901 tttattcaaa aataatacat gactctaatg tttcttttcg ttctgcaatg gatcaacttg
     3961 aactagctct taaacaaata agtcttggta ataatgacca agtgtccttg gaagataaca
     4021 gccaagatac atgttctgag acaaatttgg attctggttt ctcagtccag gcagtagttg
     4081 aaccacctga acctgaagta actgttttgg agcctgaaca tgttgaacaa gaaggtagaa
     4141 ataacgtgcc ttgtgattct gaagtaagcg ttgatgctaa tggctctgtc cagttagagg
     4201 ctggtcagca tagtgaaagt ggtgaaaaca gaagtcagaa ggatacagat gacacagaag
     4261 gtaagaggga tgatgctcag ggttttggaa ttacatgtga ttccaatgtc ccccagccat
     4321 tggccggcca cattgaagta gttcaggaca ttgaccattg gaaggatcat gttgacttgg
     4381 aagataagct tggtgaatct aaaaactcaa aagtaaactt tcattctgat gaaccacttc
     4441 aggctgtgac taacgcaaca caagagcctg ttgaagaaat aaatttaccg aggggacgtg
     4501 ctagtccaaa tggtaatggc tgtgagccct atggctctac aatagttcct gttacgaatg
     4561 tcattttttg ctcagtgatt caaaaaccac aacgtttgca aaaaaagtgt accagtttga
     4621 aagaaaacag cagcaatcct tgtcctgaag taaatgttga ttcctgtgat ggtcctgaag
     4681 tgagtgttga ttctaatgat ccctgtcagt cagtagcagg ccaccttcaa aaacctgaca
     4741 aggaaatgaa tctgaaggaa gaccatattt acctggaaga taagagctac aagctagttg
     4801 actttgaacc aacttatgat tctgacgatc ctgttcagtt tgtgacagtt ccatctgtag
     4861 aggctgtgtc tatcaaagaa gtaaacttgc aaaaggagaa tcctgatgac ctagaaaatg
     4921 agaactttca gccatgttgt tctgaagtag catgtaattc tgctgttcat ctgcagtcag
     4981 aagctgaccc acctcaagtg gcttgcaaag aagcagacct tgacaagaaa gcgcttgaca
     5041 tagaagacaa gggcagtgta acttgtgtgc ctgaagtggt gtatgattct gacgtctctt
     5101 ttcagatagt agttaaccag gttcagacat cagatggaga aacagactca ccacaggtgg
     5161 tgtttgtgga tgttgtgtcc agtgatagtg actgtgaccg ggaagtaatt tctgattcaa
     5221 atatccctct tcagctagag ccacctcaga tgactgtcaa agaaaccagt gatataaaca
     5281 cagattctct tggttctgca gcaaacgaaa agtactattg taaattctgt ggttgtgatt
     5341 atgaagcctc tcagtcagtg acaaaccaat ccaaggaaag ttttaaaata ataaacagga
     5401 aaaatgacta tattatccta ggcgattcta cttgtccatc ttgtggtcat gaactcaatt
     5461 tcaatgttga tccttctgat cagcccacta cctgccagtt acaacagcct gatcgtaatt
     5521 ttattgaccc agaagataag aactttggat ctaaatgtcc taaaagaaaa ttaaattggg
     5581 aagatactgc tcatccagtg actcaccaac tacagaaaac tggagaagcc accagtcttc
     5641 ggaaagatca aaaaaataga ttcaaaagag ataggggctg ggaatccagt agttttgcag
     5701 gagaccatgc tgcctatgct gtgtcagtga ttcgccaaac agctttgaac tcctgtggat
     5761 ctgagctaaa ctttcaagat gataccttct atcactctga cattgaccca cctcaaccta
     5821 agaaaaaagg ccagggtaag aaagtgactt ttgacttgag agtaactaag tatgaatatc
     5881 caccaaaccc aatgtacggg gaaggagaag aggttgcaga agatgatctt aaggaagtag
     5941 tagttcatga agcctcacct cagggtcagg ctcctccatc aattgttgga aaaacatgtc
     6001 ctcaggggag agaagatgat gtgaagacaa acacacaggc atgtcaaggt tatttctata
     6061 gttactatga tggtggctct gagaccaaga aaattctttt gggtgaagaa gaaaagacca
     6121 ttaggtctga ccttaatcag aacaccacat caattcaaca tgttaaaggt aaagttggag
     6181 acactggtga cttttcagtg gacttaggta aacagtcttg ttccttagca gaggggcttc
     6241 atcagcaaca tgggcaggtg acttctcaga accaagtaga agtgagatgt ggaattcaag
     6301 caagttctgg gaagaaaagg aaaattacag aacaagaaga ggactcacca aagaggaagt
     6361 gtttccacca tgatagccag aagaagaaaa aggctcaagc tgggataact gagctacctg
     6421 aaccacaaac taaagtttta gagcctgtac agcccgattc cttggtctat attttttctt
     6481 ctctaagtat gaaggaagat cagtctttga atccacctaa aacaaagcct ggcagtgaca
     6541 gtgatttgcc acatatatac agttgtagag aacacacctc ttctggccca cgacgtaaga
     6601 gaactgtaat taatcctcca cagaacctaa tggtaccaga agttggcatt gatcttaata
     6661 ggcatgaccc aaagcccaat gcaggagacg atagtgccaa aagacaaaac ctggtttcaa
     6721 catcttttat ggcaatgccg aaaaaatctg tgttaaagtt ccagaggacc aatcaatctt
     6781 catttctaaa aaaatcagaa gatgtgggtg ctactcaagt tccaaaggat aatttccagc
     6841 agactcttgt aaatcgtgat ggtgccaaaa aatttcccaa atcagttaaa aaggaagatc
     6901 ttgaaagtca aaatcaaagg aaattttgga aaaagaagat ggttgctgca aataaattat
     6961 gcctcatcaa aaatgcgtat aaaacaatgg ttctccggaa aaaatcaaaa ctagcttcag
     7021 aaaagctggc tatttggatt cagctgaaag cgaccgacat catcaggaag tatgtctcta
     7081 gatgccatgg tcttatgccc cggaggcatc tatcaaagac tgttcttata agaatgcaac
     7141 tgaggaagaa gaaaatagtt gccaggaaga taaaggaagc caagagagca gctgaagcac
     7201 tagcgctcaa gctctcgcgc ccctcgtgtc ccccgcgggc cccgtgcccc ccgcgggccc
     7261 tgtgcccccc tcgcgccccg tgccccccgc gggccctgtg cccccctcgc gccccgtgcc
     7321 ccccgcgggc cctgtgcacc cctcgcgccc cgtgcccccc gcgggccctg tgcacccctc
     7381 gcgctccgtg ccccccgcgg gccctgtgca cccctcgcgc cccgtgcccc ccgcgggccc
     7441 tgcgcgcccc ctgcccccca cgggctctgt gctccccgcg cgccccgtgc cccccgcggg
     7501 ccctgtgccc ccctcgtgcc ccgtgccccc cgcgagcccc gcgggccccg tgccctccgc
     7561 gggccccgtg ccctccgcgg gccccgtgcc tcccgcgggc tccgtgcccc ccgcgcgctc
     7621 cgtgcccccc gcgcgccccg tgcctcccac gcgccccgtg cctcccgcag gctccgtgcc
     7681 ccccgcgggc cctgtgcctc ccgcgggttt cgtgcccccc gctccccctg tgcgccccgt
     7741 gccccccgtg cgccccggtt ctcccagctg gtgctgaaga gcagttgagc actactgcag
     7801 gacctgcaga gctgcctgct catctttcca atgctggagg aataaagcgc tacaggaaaa
     7861 cttacttccg gagaaggaag agacttcttc cagtgagaga atatgacctg agaagcttga
     7921 gttccacaac aaataccgac aggatggtga cacggcttgc aagcaagtcg aaaagcaatg
     7981 aggcaaaata aaagtgcgcg tcgcgtcgta gcggatggag acctcagtcc ttcacttgga
     8041 tggaacacat ttgagacttg aatgcacata cttttcccag ctttggtggg gaaactgacc
     8101 ttacactatt ttgctacaga tattattttt cggagatttt atattcattt aaaattagtg
     8161 tttagggtcc tttttttttc ttttaaagat ttaaaagcaa cctttgtccc atttatgtag
     8221 aagagcttca tcttaatttg tctcataatt tagcatacta tatagaattc tgttccttaa
     8281 atcatgtcct ctgaaaatct ttcgtgccaa aaagttataa cactgggaga atgaacatag
     8341 caagcttatt aatttcatct tttaaacaat tgggaccgat atctatttac ttagttacca
     8401 cacttccaag ttgaatagcc agtaggtatt ctaactggat aaataaaaag agcgacattg
     8461 tgctatctcg gttttcaagt atctcagttt tgtgatcaaa cgatcacaaa aatatataac
     8521 ttctttttct tttaaagtca ttccataaaa tatttaatcc agttttttta agcacctggt
     8581 gaaagacaaa ttctttttca tttaaattgg atctgcagtt ctgtagcttt tctcatttgc
     8641 acacataccc acagatcttt ttgttaacat tattaagtct tacgtggaaa agattttgca
     8701 aatgtgaata agctttgaag gtaccttttc tccctttatt agtaatctaa aatgaaaatc
     8761 caggtaattt tatcatctta tttactaata ttttggataa catttttaaa atgatgagct
     8821 taagctgttg gtttgataat ggcatgtgga ctggccatta attgtcatta gtgaaaaact
     8881 tgacatttca gaaaaattaa gttaaactag taacctaact agagacctac ttttcaagtg
     8941 aaaaacagtt tatacatcag tttatcttaa actatctaat tatattaaac tagctagata
     9001 gttaaaggtt aagccaattt gacattttgt cttaaactac ctaattgagt ttaacagttg
     9061 agataaagag cttggataca cagtgtgaac ctctgtggta cttgagtcta ttcctttgag
     9121 ggtcattgac tgagctggtg cagatgcatc cccttttctt gttcagtcaa gacctggatc
     9181 tttttctgtc attgatgtta tgaggaaaca cgaagtttcc ctttactgtg taactactat
     9241 attttattga aactagagtc agtgttaaga tgtgtcttga tttcagagat aaaactgtgt
     9301 taccttagaa ttaatgaaat atcaacgtgg ttttgttcca ggggccatta tttaacctgt
     9361 gagtcgttaa tggtgatact atagtcatct ctttacttct aaatataagt ggtaattata
     9421 ttgcagagtg gacaaatgtg cttttttagt cggcaagtaa cgtgtttaat tttttcaacc
     9481 ttgcctcttt atcttacaaa cattttaatt tctttaaaac attcttaatg ggctgctagt
     9541 ttttttttaa atcctcaaaa tttaattaag gaagaaaact ctttcactga ttatgaagta
     9601 aacatgaaaa agtattcttt gtaggtatta atgtcttttg cagctcccag acaccattaa
     9661 taaacactct tgtaatggag gttaaaagtg agagttcata cctaaataac ctaccatgta
     9721 attatgccag attatatcta ttgtgattat gtcccgcaat gaatattcaa ataatcaaaa
     9781 tttaaagtta aaaaattcat aatttggaaa cttgtctaag gaccttttaa agggaaagca
     9841 aggaaaaaat taaaatgtaa aattaacatc accaaaaata taaaaatgtc tggtacagaa
     9901 attatggctt tgtttagcat tttgcaaata tttactaact tcttgtaagg attattattt
     9961 tagacaagat tggaaactta cttaaaatgg taaaacttaa gttaaaagtg aattatttta
    10021 gaatttgata ggatataaga tgtgcccctt tgtctacata aatgttgatt taattttcag
    10081 gctaggttga aacaattggt tattttgtgt agtcaagagt gcattatatt ctttcaatag
    10141 taatacatca aattacaacc atatcatttc attaatcttg tcccttttag tatcttgaat
    10201 agatttttat tgtgattgtc ctggtgaaac agtgtgtgga caatattgtg taaagagtgg
    10261 tttttttgca tttgtgcatt ttaaattatt tatgtaacta gggctctgag taacacgttt
    10321 tgttttgttt tgttttgttt tgcttaaaga aaagctatat ttaaatattc aaataattat
    10381 atatttccat gtaaaactaa tgtgaagacc gatgtatttt ttataatgca atttaaagtg
    10441 taaacttgtt ttgatgttat caagataaat ttattaaata ttgaaatttg tgtgtactct
    10501 ctcctaatgt tagaaccttt ttctggaaat acttaaaaag aatcagagag taattaaggc
    10561 tgaagtaatt ctgtaaaagg cctgagagat gataaataag agaatgcaaa tgttgatgct
    10621 ggcgagaaaa ttgtttaacc aaagatacat aggttgtact cacataagta attatttcaa
    10681 aagattaaac aaccactaat ttgcctggag gagtgatggg gcagctgttt gactagcacg
    10741 cctgcactga aaggattgag ttcaggaggc aggatccata ccaccatctg acgccctctg
    10801 ggaaatgacc aaccccaaag ccttccatct ctctccgtgt cttctggtgc catagcatct
    10861 gacagtgatg acaggatggc atggacaggt gcctgagaga atagtgacat aagtgcttct
    10921 gaccctagaa ttactgactt ggaacatttg ttggtcaggg attttcgagt gagaattatc
    10981 ttcctattta caatgagaag ttactacttt ttagtaaagg acttttatgt ttagaaacat
    11041 ggaattatag aattatacac caaaatgcca actcaagtaa attcaaagtg aggagacata
    11101 gctttttttc atctcttact cagatgaact caaatattga gggaaaaatt agttaaaaca
    11161 tttccctttc ttcttatact tcatgtgtgt aaagttctaa gtgttttaag ccatgggctt
    11221 aatttttcaa aattatttgc tttaaataaa gattgaattc aggataaagt atagtttgtt
    11281 gctggatctt tatattattt ctaggtttat ttattttaat gacttttaaa cctggaaatc
    11341 agtttatttc ccagatttat tattcccaaa tttagtatat gtacatattg aggtgatctt
    11401 gagtacagca cagtgtccca aactttaact gtatcatgtc ccttgatctt tgtatttggg
    11461 agccttgttg gatactataa cgatcactgt gaagtgagaa cattgaggca tagagtagtt
    11521 aacccccttc ctacccgtag caatgggcca tcttctagtc tctatcccct tagagtcatc
    11581 ttctctgttt acttcatata agcagtacct ttatgtttct ctctctctct ctctcctttg
    11641 tttcatttat catgattagg tgttttgttg ttggttagtt ggttttgaga gtctcctagt
    11701 tatagcatgc tgagcatttt gggttttaaa attacttttt agcagttagt tttaaatcta
    11761 cttaattttt tttatatatt tatgcctctg agagttgaat ccagggcctt gtgcatgtga
    11821 atcaagtgct ctgctgcaat gtacctccag ctctcagtct tgaatgacac cactaccaga
    11881 acctttctct cctgccaaca gacgggaaag ccagcaggtg cgaggctagc tttgttccct
    11941 gcatggttct tattatctga ttcctcctca gatccaagac tggacagcag agttgccttt
    12001 tagagtgttc ctgtggttca cttgagtgtt gtatgctctc gtggcctttg actcctcttt
    12061 acctacccat catgccactc ttccctgtgc tcagttctca ttttcaccat caacacatca
    12121 cctcatgctc tcaatccttt cacggtggag tgcttgcagg taactgagaa caagaaccaa
    12181 caaattgctg ggttttaatc cagttgtgcc acagagccag gaagtgtgtt ggcagcctct
    12241 tggttaagtt gctctcctgc atatttagat acttactgtc aaaatcagga agtcagcatt
    12301 gatctcacct atctactctg cagaccgtag tcaaattttg aaaattatgt caaaatgccc
    12361 cttgtaggtc ctggatttga tccacagtta tggacttcat agatactttg ttgtttcctc
    12421 agtgtggagc agtttctcgc ctgtctgtac ttggtagcat ggcattttga aggtactggt
    12481 ttatttgtgg aatatccttg agtttgagct tgtctgatgt ttctttgtaa tctatgcatt
    12541 tttggaattt gaaatttgtg ttctcagtgt gtattaacag aagccatgtg atgtcagttt
    12601 gaataaatgt ggtgatagaa t
//
