LOCUS       NM_001289766            2255 bp    mRNA    linear   ROD 10-MAR-2023
DEFINITION  Mus musculus zeta-chain (TCR) associated protein kinase (Zap70),
            transcript variant 4, mRNA.
ACCESSION   NM_001289766 XM_006495895
VERSION     NM_001289766.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Park J, Lee SY, Jeon Y, Kim KM, Lee JK, Ko J, Park EJ, Yoon JS,
            Kang BE, Ryu D, Lee H, Shin SJ, Go H and Lee CW.
  TITLE     The Pellino1-PKCtheta; Signaling Axis Is an Essential Target for
            Improving Antitumor CD8+ T-lymphocyte Function
  JOURNAL   Cancer Immunol Res 10 (3), 327-342 (2022)
   PUBMED   35058288
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Nakamura A, Zeng F, Nakamura S, Reid KT, Gracey E, Lim M, Leng L,
            Jo S, Park YS, Kusuda M, Machhar R, Boroojeni SF, Wu B, Rossomacha
            E, Kim TH, Ciccia F, Rockel JS, Kapoor M, Inman RD, Jurisica I,
            Crome SQ, Bucala R and Haroon N.
  TITLE     Macrophage migration inhibitory factor drives pathology in a mouse
            model of spondyloarthritis and is associated with human disease
  JOURNAL   Sci Transl Med 13 (616), eabg1210 (2021)
   PUBMED   34669443
  REMARK    Erratum:[Sci Transl Med. 2021 Nov 17;13(620):eabn0903. PMID:
            34788077]
REFERENCE   3  (bases 1 to 2255)
  AUTHORS   Nguyen TTT, Wang ZE, Shen L, Schroeder A, Eckalbar W and Weiss A.
  TITLE     Cbl-b deficiency prevents functional but not phenotypic T cell
            anergy
  JOURNAL   J Exp Med 218 (7) (2021)
   PUBMED   33974042
REFERENCE   4  (bases 1 to 2255)
  AUTHORS   Dine E, Reed EH and Toettcher JE.
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate
            LAT acts as a clustering-dependent signaling switch
  JOURNAL   Cell Rep 35 (12), 109280 (2021)
   PUBMED   34161759
  REMARK    GeneRIF: Positive feedback between the T cell kinase Zap70 and its
            substrate LAT acts as a clustering-dependent signaling switch.
REFERENCE   5  (bases 1 to 2255)
  AUTHORS   Kuroyama H, Ikeda T, Kasai M, Yamasaki S, Tatsumi M, Utsuyama M,
            Saito T and Hirokawa K.
  TITLE     Identification of a novel isoform of ZAP-70, truncated ZAP kinase
  JOURNAL   Biochem Biophys Res Commun 315 (4), 935-941 (2004)
   PUBMED   14985102
REFERENCE   6  (bases 1 to 2255)
  AUTHORS   Ishijima SA, Zeng YX, Kurashima C, Utsuyama M, Shirasawa T,
            Sakamoto K and Hirokawa K.
  TITLE     Expression of ZAP-70 gene in the developing thymus and various
            nonlymphoid tissues of embryonic and adult mice
  JOURNAL   Cell Immunol 165 (2), 278-283 (1995)
   PUBMED   7553892
REFERENCE   7  (bases 1 to 2255)
  AUTHORS   Negishi I, Motoyama N, Nakayama K, Nakayama K, Senju S, Hatakeyama
            S, Zhang Q, Chan AC and Loh DY.
  TITLE     Essential role for ZAP-70 in both positive and negative selection
            of thymocytes
  JOURNAL   Nature 376 (6539), 435-438 (1995)
   PUBMED   7630421
REFERENCE   8  (bases 1 to 2255)
  AUTHORS   Gauen LK, Zhu Y, Letourneur F, Hu Q, Bolen JB, Matis LA, Klausner
            RD and Shaw AS.
  TITLE     Interactions of p59fyn and ZAP-70 with T-cell receptor activation
            motifs: defining the nature of a signalling motif
  JOURNAL   Mol Cell Biol 14 (6), 3729-3741 (1994)
   PUBMED   8196616
REFERENCE   9  (bases 1 to 2255)
  AUTHORS   Chan AC, van Oers NS, Tran A, Turka L, Law CL, Ryan JC, Clark EA
            and Weiss A.
  TITLE     Differential expression of ZAP-70 and Syk protein tyrosine kinases,
            and the role of this family of protein tyrosine kinases in TCR
            signaling
  JOURNAL   J Immunol 152 (10), 4758-4766 (1994)
   PUBMED   8176201
REFERENCE   10 (bases 1 to 2255)
  AUTHORS   Ku G, Malissen B and Mattei MG.
  TITLE     Chromosomal location of the Syk and ZAP-70 tyrosine kinase genes in
            mice and humans
  JOURNAL   Immunogenetics 40 (4), 300-302 (1994)
   PUBMED   8082894
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC084389.1.
            
            [WARNING] On May 3, 2023 this sequence was replaced by
            NM_001289766.2.
            
            On Jan 31, 2014 this sequence version replaced XM_006495895.1.
            
            Summary: This gene encodes a member of the protein tyrosine kinase
            family. The encoded protein is essential for development of T
            lymphocytes and thymocytes, and functions in the initial step of T
            lymphocyte receptor-mediated signal transduction. A mutation in
            this gene causes chronic autoimmune arthritis, similar to
            rheumatoid arthritis in humans. Mice lacking this gene are
            deficient in alpha-beta T lymphocytes in the thymus. In humans,
            mutations in this gene cause selective T-cell defect, a severe
            combined immunodeficiency disease characterized by a selective
            absence of CD8-positive T lymphocytes. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (4) encodes the longest isoform
            (b).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC084389.1         39136-39288         c
            154-564             AC084389.1         32591-33001         c
            565-725             AC084389.1         31854-32014         c
            726-864             AC084389.1         25512-25650         c
            865-949             AC084389.1         25339-25423         c
            950-993             AC084389.1         25213-25256         c
            994-1045            AC084389.1         25001-25052         c
            1046-1241           AC084389.1         24491-24686         c
            1242-1448           AC084389.1         23912-24118         c
            1449-1641           AC084389.1         22774-22966         c
            1642-1782           AC084389.1         22544-22684         c
            1783-1895           AC084389.1         22345-22457         c
            1896-2255           AC084389.1         20983-21342         c
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 15.41 cM"
     gene            1..2255
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /note="zeta-chain (TCR) associated protein kinase"
                     /db_xref="GeneID:22637"
                     /db_xref="MGI:MGI:99613"
     exon            1..153
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..2019
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /EC_number="2.7.10.2"
                     /note="isoform b is encoded by transcript variant 4;
                     tyrosine-protein kinase ZAP-70; syk-related tyrosine
                     kinase; 70 kDa zeta-chain associated protein"
                     /codon_start=1
                     /product="tyrosine-protein kinase ZAP-70 isoform b"
                     /protein_id="NP_001276695.1"
                     /db_xref="GeneID:22637"
                     /db_xref="MGI:MGI:99613"
                     /translation="MQICLGKPGRSLLPSVVCLLQGPAMPDPAAHLPFFYGSISRAEA
                     EEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHC
                     GPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGD
                     ALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKE
                     QGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKE
                     VCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMD
                     TSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIK
                     VLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLL
                     GKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKA
                     LGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKM
                     KGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLA
                     SRAEGPPQCEQVAEAACG"
     exon            154..564
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            565..725
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            726..864
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            865..949
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            950..993
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1045
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1241
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1448
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1641
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1782
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1895
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2255
                     /gene="Zap70"
                     /gene_synonym="mrtle; mur; Srk; ZAP-70"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcatccttg gccctgtctc agagcatccg ggagcccagc tttctggccg ggtccatacc
       61 accattctct aggagatctg agaccctggg atgcaaatct gtcttggaaa accaggaagg
      121 agcctgttac cctctgtggt gtgcttacta cagggtccag cgatgcccga tcccgcggcg
      181 cacctgccat tcttctatgg cagcatctcg cgggctgagg ccgaggagca cctgaagctg
      241 gcaggcatgg ccgacgggct gttcctcctg cgccagtgtt tgcgctccct gggcggctac
      301 gtgctgtcgt tggtgcacga cgtgcgcttc caccatttcc ccatcgagcg ccaactcaac
      361 ggcacgtacg ccatcgcggg cgggaaggcg cactgcggcc cggccgagct ctgccagttc
      421 tactctcagg accccgacgg gctgccctgc aacctgcgta agccgtgtaa ccggccgccc
      481 ggactggagc cacagcccgg ggtcttcgac tgcctgcgtg atgctatggt gcgcgactac
      541 gtgcgccaga cctggaagct ggagggcgat gcactagagc aggccatcat cagccaggcc
      601 ccacaggtgg agaagctcat tgctaccaca gcccacgagc gaatgccctg gtatcacagc
      661 agcctgactc gtgaggaggc cgagcgcaaa ctctattccg gccagcagac cgacggcaag
      721 ttcctgctga ggccccggaa ggagcagggc acatatgcac tgtccctggt ctatgggaaa
      781 actgtatacc actatctcat cagccaggac aaggctggca agtactgcat ccccgaaggc
      841 accaagtttg acacgctctg gcagctggtg gagtacctga agctgaaggc ggatggacta
      901 atttaccgct tgaaggaggt ctgtcccaac agcagcgcca gcgcggcagt tgctgcaccc
      961 accctcccag cccacccatc cacattcact cagcctcaga gacgagtgga caccctgaac
     1021 tcggacggat acacccctga accagcacgc ttagcgtcat cgacagacaa gccccggccg
     1081 atgcccatgg acacaagtgt gtacgagagt ccctacagcg accctgagga actcaaagac
     1141 aagaagctct tcctgaagcg agagaatctc ctcgtggcgg acatcgagct tggctgtggc
     1201 aactttggct ccgtgcgcca gggagtctat cgcatgcgca agaagcagat tgacgtggcc
     1261 atcaaggtgc tgaagcaggg cacagagaag gccgacaaag atgagatgat gcgagaggcc
     1321 cagatcatgc accagctcga caacccctac atcgtgcggc tcatcggcgt gtgccaggca
     1381 gaagcactca tgctggtcat ggagatggcg ggaggcgggc ccctgcacaa gttcctgctg
     1441 ggaaagaagg aggagatccc tgtgagcaat gtggctgaac tgctgcacca ggtggccatg
     1501 ggcatgaagt atttggagga gaaaaacttt gtgcaccgcg acctggcagc ccgcaatgtt
     1561 ctactggtca atcggcacta tgccaagatc agcgactttg gcctgtccaa agccctgggt
     1621 gctgacgaca gctattacac agcccggtct gcagggaagt ggcctctgaa gtggtacgcg
     1681 ccagagtgca tcaactttcg gaagttctcc agccgcagtg acgtctggag ctatggggtc
     1741 accatgtggg aggccttctc ctatggccag aagccctaca agaaaatgaa gggccccgag
     1801 gtcctggact tcatcaagca gggtaagagg atggaatgtc cgccggagtg tcctcctgag
     1861 atgtatgcac ttatgagtga ctgctggatc tacaagtggg aggatcgccc cgacttcctg
     1921 actgtggaac aacgtatgcg gaactattac tacagcctgg ccagccgggc cgagggaccc
     1981 ccacagtgtg aacaggtggc cgaggctgca tgtggctgag ccccaagcct ccctacaccc
     2041 tctgtccagg atgacctgtg ttcaggagcc ctatcgactg tcccatgtga ttcgccccgc
     2101 cccatgggct ggggctgatg ggatattggt aaacatcacc agtgggctga gaggatgtgt
     2161 gctcccaaag tctacttccg ctgtttctga gtgggagccc agagtcctcc aggggtcact
     2221 gtcagcatca gaataaactc cacttctctt ggctg
//
