LOCUS       NM_145828               3424 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus xylosyltransferase II (Xylt2), mRNA.
ACCESSION   NM_145828
VERSION     NM_145828.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ferencz,B., Condac,E., Poudel,N., Munteanu,M.C., Sivasami,P.,
            Choudhury,B., Naidu,N.N., Zhang,F., Breshears,M., Linhardt,R.J. and
            Hinsdale,M.E.
  TITLE     Xylosyltransferase 2 deficiency and organ homeostasis
  JOURNAL   Glycoconj J 37 (6), 755-765 (2020)
   PUBMED   32965647
  REMARK    GeneRIF: Xylosyltransferase 2 deficiency and organ homeostasis.
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Mohan,R.A., Bosada,F.M., van Weerd,J.H., van Duijvenboden,K.,
            Wang,J., Mommersteeg,M.T.M., Hooijkaas,I.B., Wakker,V., de Gier-de
            Vries,C., Coronel,R., Boink,G.J.J., Bakkers,J., Barnett,P.,
            Boukens,B.J. and Christoffels,V.M.
  TITLE     T-box transcription factor 3 governs a transcriptional program for
            the function of the mouse atrioventricular conduction system
  JOURNAL   Proc Natl Acad Sci U S A 117 (31), 18617-18626 (2020)
   PUBMED   32675240
REFERENCE   3  (bases 1 to 3424)
  AUTHORS   Sivasami,P., Poudel,N., Munteanu,M.C., Hudson,J., Lovern,P.,
            Liu,L., Griffin,T. and Hinsdale,M.E.
  TITLE     Adipose tissue loss and lipodystrophy in xylosyltransferase II
            deficient mice
  JOURNAL   Int J Obes (Lond) 43 (9), 1783-1794 (2019)
   PUBMED   30778123
  REMARK    GeneRIF: Using a xylosyltransferase II (Xylt2) deficient mouse
            model, studied the role of extracellular matrix sulfated
            glycosaminoglycans in adipose tissue and metabolic homeostasis.
REFERENCE   4  (bases 1 to 3424)
  AUTHORS   Munns,C.F., Fahiminiya,S., Poudel,N., Munteanu,M.C., Majewski,J.,
            Sillence,D.O., Metcalf,J.P., Biggin,A., Glorieux,F., Fassier,F.,
            Rauch,F. and Hinsdale,M.E.
  TITLE     Homozygosity for frameshift mutations in XYLT2 result in a
            spondylo-ocular syndrome with bone fragility, cataracts, and
            hearing defects
  JOURNAL   Am J Hum Genet 96 (6), 971-978 (2015)
   PUBMED   26027496
REFERENCE   5  (bases 1 to 3424)
  AUTHORS   Condac,E., Dale,G.L., Bender-Neal,D., Ferencz,B., Towner,R. and
            Hinsdale,M.E.
  TITLE     Xylosyltransferase II is a significant contributor of circulating
            xylosyltransferase levels and platelets constitute an important
            source of xylosyltransferase in serum
  JOURNAL   Glycobiology 19 (8), 829-833 (2009)
   PUBMED   19389916
  REMARK    GeneRIF: the data from Xylt2 knock-out mice and mice with liver
            neoplasia show that liver is a significant source of serum XylT2
            activity.
REFERENCE   6  (bases 1 to 3424)
  AUTHORS   Condac,E., Silasi-Mansat,R., Kosanke,S., Schoeb,T., Towner,R.,
            Lupu,F., Cummings,R.D. and Hinsdale,M.E.
  TITLE     Polycystic disease caused by deficiency in xylosyltransferase 2, an
            initiating enzyme of glycosaminoglycan biosynthesis
  JOURNAL   Proc Natl Acad Sci U S A 104 (22), 9416-9421 (2007)
   PUBMED   17517600
  REMARK    GeneRIF: alterations in proteoglycans (PGs)concentrations can occur
            due to loss of XylT2, and that reduced PGs can induce cyst
            development
REFERENCE   7  (bases 1 to 3424)
  AUTHORS   Wang,M., Zhang,Z., Zhang,Z., Vikis,H., Yan,Y., Wang,Y. and You,M.
  TITLE     Fine mapping and candidate gene analyses of pulmonary adenoma
            resistance 1, a major genetic determinant of mouse lung adenoma
            resistance
  JOURNAL   Cancer Res 67 (6), 2508-2516 (2007)
   PUBMED   17363568
REFERENCE   8  (bases 1 to 3424)
  AUTHORS   Cuellar,K., Chuong,H., Hubbell,S.M. and Hinsdale,M.E.
  TITLE     Biosynthesis of chondroitin and heparan sulfate in chinese hamster
            ovary cells depends on xylosyltransferase II
  JOURNAL   J Biol Chem 282 (8), 5195-5200 (2007)
   PUBMED   17189266
  REMARK    Erratum:[J Biol Chem. 2007 Jun 22;282(25):18660]
REFERENCE   9  (bases 1 to 3424)
  AUTHORS   Schon,S., Prante,C., Bahr,C., Kuhn,J., Kleesiek,K. and Gotting,C.
  TITLE     Cloning and recombinant expression of active full-length
            xylosyltransferase I (XT-I) and characterization of subcellular
            localization of XT-I and XT-II
  JOURNAL   J Biol Chem 281 (20), 14224-14231 (2006)
   PUBMED   16569644
REFERENCE   10 (bases 1 to 3424)
  AUTHORS   Gotting,C., Kuhn,J., Zahn,R., Brinkmann,T. and Kleesiek,K.
  TITLE     Molecular cloning and expression of human UDP-d-Xylose:proteoglycan
            core protein beta-d-xylosyltransferase and its first isoform XT-II
  JOURNAL   J Mol Biol 304 (4), 517-528 (2000)
   PUBMED   11099377
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK158056.1, AK150521.1 and AK083482.1.
            
            On Jan 18, 2008 this sequence version replaced NM_145828.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150521.1, AK153332.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AK158056.1         1-48
            49-1184             AK150521.1         2-1137
            1185-1991           AK158056.1         1185-1991
            1992-3211           AK150521.1         1945-3164
            3212-3424           AK083482.1         3182-3394
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 58.9 cM"
     gene            1..3424
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /note="xylosyltransferase II"
                     /db_xref="GeneID:217119"
                     /db_xref="MGI:MGI:2444797"
     exon            1..221
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2684
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /EC_number="2.4.2.26"
                     /note="peptide O-xylosyltransferase 2"
                     /codon_start=1
                     /product="xylosyltransferase 2"
                     /protein_id="NP_665827.2"
                     /db_xref="CCDS:CCDS48888.1"
                     /db_xref="GeneID:217119"
                     /db_xref="MGI:MGI:2444797"
                     /translation="MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEPG
                     EKGRQRKPRPLDPGEGSKDTDSSAGRRGSAGRRHGRWRGRAESPGVPVAKVVRAVTSR
                     QRASRRVPPAPPPEAPGRQNLSGAAAGEALIGAPGFPQHGDTGSVEGAPQPTDNTFTP
                     KCEIVGKDALSALARASTKQCQQEIANVVCLHQAGNLMPKSVPRHCQLAGKMSPGVQW
                     EEIRAQQPVGGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSNYLYRE
                     VVELAQHYENVRVTPWRMVTIWGGASLLRMYLRSMKDLLEIPGWTWDFFINLSATDYP
                     TRTNEELVAFLSKNRDKNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAG
                     IVVDGGSDWFVLTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAESFFHTVLENSPACAS
                     LVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFLRLQQVSRPTFFARKFES
                     TVNQEVLEILDFHLYGSYPPSTPALKAYWENIYDVADGPGGLSDVLLTAYTAFARLSL
                     RHAATAVSPLATAVCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMW
                     LMPQRSLKLLGHSDQASRLQSLEVGTEWDPKERLFRNFGGLLGPLDEPVAMQRWARGP
                     NLTATVVWIDPTYVVATSYDITVDADTEVTQYKPPLSLPLRPGAWTVRLLQFWEPLGE
                     TRFLVLPLTFNRKLPLRKDDASWLHAGPPHNEYMEQSFQGLSGILNLPQAEALEEAAR
                     RHTELTGSALEAWTDGELSNFWSVAGLCAIGPSACPSLELCRLTSWSSLSPDPKSELG
                     PVKADGRLR"
     exon            222..714
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            715..890
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1093
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1174
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1391
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1568
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1831
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..2027
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2361
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..3424
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3400..3405
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /note="hexamer: AATATA"
     polyA_site      3420
                     /gene="Xylt2"
                     /gene_synonym="E030002B02Rik; XT-II; XT2; xylT-II"
                     /note="major polyA site"
ORIGIN      
        1 ggagggactc cggggccccg cgcctgtgcg cacgcccgcg gccggtttgc agggccggac
       61 gcgcgccccg cgtcccgggc aggaagatgg tggcgagcgc gcgggtgcag aagctggtgc
      121 ggcgctacaa gctggcgatc gccaccgcac tggccatcct gctgctgcag ggcctggtgg
      181 tgtggagctt cagcggcctg gaggaggacg agccgggcga gaaagggagg cagaggaagc
      241 cacggccact agaccctggg gagggctcta aggacacaga cagctctgcg gggcgtaggg
      301 gcagcgctgg gagaaggcac ggacgctggc ggggccgcgc tgagagccca ggtgtgcccg
      361 tggccaaggt ggtgcgggca gtaaccagtc ggcagagggc cagccgacgg gtgcccccgg
      421 ccccacctcc ggaggcacca ggccgccaga acctgagcgg agcggcggcc ggggaagcac
      481 tgataggggc cccaggcttc ccacagcatg gagacacagg gagtgtggag ggtgcccccc
      541 agcccacgga caacaccttc actccgaagt gtgagattgt gggcaaggac gcactgtcag
      601 cactggcccg ggccagcacc aagcagtgtc aacaggagat cgctaatgta gtgtgcctgc
      661 accaagctgg gaacctaatg cccaagtctg tgccccggca ctgccagctg gctggcaaga
      721 tgagccctgg cgtccaatgg gaagagatcc gggcccagca gcctgttggt ggccctccag
      781 tacgcatcgc ctacatgctg gtggttcacg gccgtgctat ccgccagctg aagcgtcttc
      841 tgaaggccgt ctaccatgag caacacttct tttatataca tgtggacaag cgttccaact
      901 acctgtaccg ggaggtagtg gaactggccc agcactacga gaatgtgcgg gtgacacctt
      961 ggcgcatggt caccatctgg ggcggggcca gccttctgag gatgtatctg aggagcatga
     1021 aggaccttct ggaaattcct ggctggacct gggacttctt catcaacctg agtgccactg
     1081 actatccaac gaggacgaat gaggagctgg tagcattctt atccaagaac cgggacaaga
     1141 atttcctcaa gtcacatggg cgagacaatt ccaggttcat taagaagcaa ggcctggacc
     1201 ggcttttcca tgagtgtgat tcccacatgt ggcgcctggg tgaacggcag atcccggcag
     1261 gcattgtggt ggatggtggc tctgactggt ttgtgctgac acgcagcttt gtggaatatg
     1321 tggtgtatac agatgacccc ctcgtggccc agcttcgcca gttctataca tacacattgc
     1381 ttccggctga gtcattcttc cacacagtgc tggagaacag cccggcctgt gcgagcctgg
     1441 tggacaacaa cctgcgggtc accaactgga accgcaagct gggctgcaaa tgccagtaca
     1501 agcacatcgt ggactggtgc ggctgctccc ccaacgactt caagccacag gacttcctga
     1561 ggcttcagca agtctccaga cccaccttct ttgcccggaa gttcgagtcg actgtgaacc
     1621 aggaagtcct ggaaattttg gacttccatc tatatggcag ctacccaccc agcacaccag
     1681 ccctcaaagc ctactgggag aacatctacg acgtggccga tggccctggc ggactcagcg
     1741 acgtcctact cacagcctac acagcctttg cccgtctcag cctgcgtcat gctgccactg
     1801 ctgtatcccc actggccact gcagtctgca ggtttgagcc cagggggttg ccgtccagcg
     1861 tgcacctgta tttctatgac gaccatttcc agggctacct ggtgacgcag gcggtgcagc
     1921 cctcagccca ggggccagca gagacacttg agatgtggct gatgccccag aggtcgctga
     1981 agctgttggg gcacagtgac caggccagcc ggctccagag tctggaggtt ggtactgagt
     2041 gggaccccaa agaacgtctc ttccggaact ttgggggcct gctgggacca ctggatgagc
     2101 ccgtagccat gcagcgctgg gcccggggcc ccaacctcac agccactgtg gtctggattg
     2161 accccaccta tgttgtggcc acatcgtatg acatcacggt agatgcggat accgaagtca
     2221 cgcagtacaa gcctccactg agcctgccac tgcggccggg agcctggact gtccgcttgc
     2281 ttcagttctg ggaacccctg ggtgagaccc gcttcctcgt gctgccgttg acgttcaacc
     2341 gcaaactacc tctcaggaaa gatgatgcca gctggctgca tgcgggacca ccccacaacg
     2401 aatacatgga gcagagtttc cagggcctaa gtggcatcct gaatctgcct caggcagagg
     2461 ccctggagga ggctgcccgg aggcacacag agctcacggg ttctgcactt gaggcctgga
     2521 cagatgggga actgagcaat ttctggtctg tggcaggatt gtgtgccata ggaccttctg
     2581 cttgtccctc cctggagctc tgcagactga ccagctggag ctctctgtct cctgacccca
     2641 agtcagagct ggggcctgtc aaagctgacg ggcgactcag gtagcaggac cccagccagc
     2701 acaacccgga ggagccgggg aattgcacct tacagacaac ggagggacat ctctcccaca
     2761 gacaagaacc agaggcccag gcagtttgcc catttgagcc aacaggaacc tacactgagg
     2821 gcagggaagg tgggtgcagt actccctact gctaacggcc ctgcaagaga ccagcggtcc
     2881 actggagcca tgaggaggat cttcctctgc tcctcgttag tttatacttt tttaataatt
     2941 aaaatgagtg gcagagggag gacgatgtgc aatagggctc agagcagccc caggatatgg
     3001 gccatggcca tggggagcac cacagagcat atcggtttct gctctgggga ggcatgggct
     3061 gcctccctct ccagagcctc catccccagc ctgcatctga actgcgcact gctgaggctc
     3121 tgaggcccgg aggacaggtg atctgtgggg gctactgtgg agttggactt tagtgagcag
     3181 gcctctcctc tgggagccag ggagccctag agcagagctg gggccggctc tcgggcttcc
     3241 ctaggaaatg cgcgccatca gggcctgctg agatccatga agtttcccta ctggaggagg
     3301 aagcccactg gcggcacagg aaggctgtct gtcagagctc tcacaagagt cgagttgtgc
     3361 ctgaagctca gtgcgaagtc tgaaatatgc aacagaggaa atatatctct atctctccaa
     3421 gctc
//
