LOCUS       NM_001311130           10266 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Mus musculus 5'-3' exoribonuclease 1 (Xrn1), transcript variant 2,
            mRNA.
ACCESSION   NM_001311130 XM_006511153
VERSION     NM_001311130.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Li,L., Krasnykov,K., Homolka,D., Gos,P., Mendel,M., Fish,R.J.,
            Pandey,R.R. and Pillai,R.S.
  TITLE     The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse
            fertility independently of m6A recognition
  JOURNAL   Mol Cell 82 (9), 1678-1690 (2022)
   PUBMED   35305312
  REMARK    GeneRIF: The XRN1-regulated RNA helicase activity of YTHDC2 ensures
            mouse fertility independently of m(6)A recognition.
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Takaoka,S., Yanagiya,A., Mohamed,H.M.A., Higa,R., Abe,T.,
            Inoue,K.I., Takahashi,A., Stoney,P. and Yamamoto,T.
  TITLE     Neuronal XRN1 is required for maintenance of whole-body metabolic
            homeostasis
  JOURNAL   iScience 24 (10), 103151 (2021)
   PUBMED   34646989
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10266)
  AUTHORS   Wojtas,M.N., Pandey,R.R., Mendel,M., Homolka,D., Sachidanandam,R.
            and Pillai,R.S.
  TITLE     Regulation of m6A Transcripts by the 3'-->5' RNA Helicase YTHDC2 Is
            Essential for a Successful Meiotic Program in the Mammalian
            Germline
  JOURNAL   Mol Cell 68 (2), 374-387 (2017)
   PUBMED   29033321
REFERENCE   4  (bases 1 to 10266)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   5  (bases 1 to 10266)
  AUTHORS   Luchelli,L., Thomas,M.G. and Boccaccio,G.L.
  TITLE     Synaptic control of mRNA translation by reversible assembly of XRN1
            bodies
  JOURNAL   J Cell Sci 128 (8), 1542-1554 (2015)
   PUBMED   25736288
  REMARK    GeneRIF: XRN1 knockdown impairs the translational repression
            triggered by NMDA. Collectively, these observations support a role
            for the SX-bodies in the reversible masking and silencing of mRNAs
            at the synapse.
REFERENCE   6  (bases 1 to 10266)
  AUTHORS   Zheng,D., Ezzeddine,N., Chen,C.Y., Zhu,W., He,X. and Shyu,A.B.
  TITLE     Deadenylation is prerequisite for P-body formation and mRNA decay
            in mammalian cells
  JOURNAL   J Cell Biol 182 (1), 89-101 (2008)
   PUBMED   18625844
REFERENCE   7  (bases 1 to 10266)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 10266)
  AUTHORS   Shimoyama,Y., Morikawa,Y., Ichihara,M., Kodama,Y., Fukuda,N.,
            Hayashi,H., Morinaga,T., Iwashita,T., Murakumo,Y. and Takahashi,M.
  TITLE     Identification of human SEP1 as a glial cell line-derived
            neurotrophic factor-inducible protein and its expression in the
            nervous system
  JOURNAL   Neuroscience 121 (4), 899-906 (2003)
   PUBMED   14580940
REFERENCE   9  (bases 1 to 10266)
  AUTHORS   Shobuike,T., Sugano,S., Yamashita,T. and Ikeda,H.
  TITLE     Cloning and characterization of mouse Dhm2 cDNA, a functional
            homolog of budding yeast SEP1
  JOURNAL   Gene 191 (2), 161-166 (1997)
   PUBMED   9218715
REFERENCE   10 (bases 1 to 10266)
  AUTHORS   Bashkirov,V.I., Scherthan,H., Solinger,J.A., Buerstedde,J.M. and
            Heyer,W.D.
  TITLE     A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4
            tetraplex substrates
  JOURNAL   J Cell Biol 136 (4), 761-773 (1997)
   PUBMED   9049243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK162932.1, BC054108.1, AK143400.1, BB202930.2, AK052194.1 and
            AC121499.9.
            
            On Jul 24, 2015 this sequence version replaced XM_006511153.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. It
            encodes isoform 2, which is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X91617.1, SRR10223970.11717.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-810               AK162932.1         1-810
            811-814             BC054108.1         712-715
            815-2026            AK162932.1         816-2027
            2027-2105           BC054108.1         1928-2006
            2106-2238           AK143400.1         2037-2169
            2239-2634           BC054108.1         2140-2535
            2635-2986           AK143400.1         2567-2918
            2987-3303           BB202930.2         311-627
            3304-4087           BC054108.1         3205-3988
            4088-5135           AK052194.1         71-1118
            5136-5494           BC054108.1         5884-6242
            5495-10266          AC121499.9         12894-17665         c
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9"
     gene            1..10266
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /note="5'-3' exoribonuclease 1"
                     /db_xref="GeneID:24127"
                     /db_xref="MGI:MGI:891964"
     exon            1..184
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..5269
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein Dhm2; strand-exchange protein 1 homolog"
                     /codon_start=1
                     /product="5'-3' exoribonuclease 1 isoform 2"
                     /protein_id="NP_001298059.1"
                     /db_xref="CCDS:CCDS81053.1"
                     /db_xref="GeneID:24127"
                     /db_xref="MGI:MGI:891964"
                     /translation="MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCS
                     HPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRF
                     RSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKS
                     WQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHE
                     AHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSALKEKITFKYDIE
                     KIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYIAILPELGGYINESGHLNLP
                     RFERYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGAAAEEAKNCKEKRKPKG
                     QENSLSWAALDKSEGEGVASRDNFEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDE
                     FLANQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIRSISTLKIHFELGKPF
                     KPFEQLLAVLPSASKNLLPTCYQHLMTSEDSPIIEYYPPDFKTDLNGKQQEWEAVVLI
                     PFIDETRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFTYSSPWPEKFPAIER
                     CCTRYKMISLDAWRVDINKNKITRVDQKALYFCGFPTLKHIKHKFFLKKSGVQVFQQS
                     SRGENLMLEISVNAEPDELRIENIASAVLGKAVFVNWPHLEEARVVAVSDGETKFYIE
                     EPPGTQKVYLGKTAPPSKVIQLTDKEQSNWTKEIQGISEQYLRRKGIIINETSAVVYA
                     QLLTGRKYQISQNGEVRLEKQWSKQILPFVYQTIVKDIRAFDSRFSNIKTLDDLFPPR
                     TMVFMLGTPYYGCTGEVQDSGDLITEGRIRVVFSIPCEPNLDALIQNQHKYSIKYNPG
                     YVLAGRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKK
                     VGNEWMYSSAAEQLLAEYIERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEI
                     ITWLKGHPVSTLSRSSCDLHILDAAIVEKIEEEVEKCKQRKSNKKVRVTVKPHLLYRP
                     LEQQHGVIPDRDAEFRLFDRVVNVRESFSVPVGLRGTVIGIKGASREADVLFEVLFDE
                     EFPGGLTIRCSPGRGYRLPTSALVNLSHGSRCETGNQKLTAIVKPQPSVSHCSAAPSG
                     HLGGLNHSPQSPFLPTQVPTKGDDEFCNIWQSLQGAGKIQHLQPTVQEKGAVLPQEIS
                     QVTEGHKSGFTDHSVRHQQRKHDSQRKFKEEYKSPKAECQSQKLSSKQTSGGSARCSI
                     KLLKRNESPGTSEAQKVVTSYPNAVHKPPSGIENFLASLNLSKENEAQLPHHGEPPDE
                     ADLSPQSFAMKGTRMLKEILKIDSPDTRDSKNDMKKSDNEATVSSRRDERGVSAHPKP
                     TCHMNKPHGTNEFQNVASVDSVCWPGQMPPVSTPVTELSRICSLVGMPQPDFSFLRTT
                     QTMTVCQVKLSNGLLVHGPQCHSESEAKERAALFALQQLGSLGVSFPLPPPIFTNYPP
                     AVPPGAVPPVFTQPTANIMPSSSHLFGSVSWRPPVPVAGNAFHYPSYPGTMPLAGGVP
                     GGVHSQFIPLQVTKKRVANRKNFENKEAQSSQATPLQTNKPGSSEATKMTPQESPPAS
                     SSSSQAAQPVSSHVETASQGHVGSQPRSAPSSSKRKSRKLAVNFSVSKPSE"
     exon            185..417
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            418..515
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            516..625
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            626..736
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            737..819
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            820..907
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1076
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1144
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1282
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1349
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1455
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1545
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1702
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1822
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1992
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2113
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2212
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2316
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2448
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2611
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2612..2725
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..2824
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2940
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3087
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3177
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3313
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3314..3370
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3503
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3573
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..3772
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3773..3868
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3869..3977
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3978..4039
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4040..4150
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4151..4273
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..4418
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4419..4594
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4595..4708
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4709..4811
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4812..4972
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     exon            4973..10266
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10234..10239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /note="hexamer: ATTAAA"
     polyA_site      10260
                     /gene="Xrn1"
                     /gene_synonym="Dhm2; exo; mXrn1"
                     /note="major polyA site"
ORIGIN      
        1 ggaccttgtg ttgtcatcct ccgagcgacg gccggaggcg gcggcggagg cccgggcggg
       61 gtgtttggtt tcgtttctgc accgactgga attagtgtcg acggtcaaaa tgggagtccc
      121 caagttttac cgatggatct cggagcggta cccctgcctc agcgaagtgg tgaaggagca
      181 tcagattcca gaatttgata acttgtacct ggatatgaat gggattatac atcagtgttc
      241 ccatcctaat gatgatgatg ttcacttcag aatttcagat gacaaaatct ttactgacat
      301 ttttcactac ttggaggtgt tgtttcgaat tattaaacct aggaaagtgt tctttatggc
      361 tgttgacggt gtggctcctc gagcaaaaat gaaccagcaa cgcgggaggc gttttaggtc
      421 agccaaggag gcagaagaca aaattaaaaa agcaatagaa aagggagaaa cacttcctac
      481 agaagccaga ttcgattcca actgtattac gccagggact gaattcatgg ccagattgca
      541 tgaacatctg aagtattttg taaatatgaa aatttccaca gacaaatcat ggcaaggtgt
      601 taccatctac ttctcaggcc atgagactcc tggagaagga gagcataaaa tcatggaatt
      661 tatcagatcc gagaaagcaa agccagatca tgatccaaac accagacatt gtctttatgg
      721 cttagatgct gacttgatca tgcttggatt aacaagtcac gaggcacatt tttctctctt
      781 aagagaagaa gttcggtttg gtggaaaaaa aacacaaagg gtatgtgcac cagaagaaac
      841 gacatttcac ctcctacact tgtctttaat gagagagtat attgattatg aattttcagc
      901 attaaaggaa aagatcacat ttaaatatga tattgaaaaa attatagatg attggatttt
      961 gatgggattt cttgttggca atgattttat ccctcatcta cctcatttac atattaatca
     1021 tgatgcgctg cctcttcttt atggaacata tattgctatc ctgccagaac ttggaggtta
     1081 tattaatgaa agtggacatc tcaacttacc tcgatttgag agataccttg tgaaactatc
     1141 agattttgat cgagaacact tcagtgaagt ttttgtggac ctcaagtggt ttgaaagcaa
     1201 agttggtaac aagtatctca atgaggcagc aggtgcagca gcagaagagg caaagaactg
     1261 caaggagaag agaaaaccga agggtcagga aaattcttta agttgggctg cattagacaa
     1321 aagtgaaggt gaaggagtag cttctagaga taattttgag gatgagactg aagatgatga
     1381 tctgtttgaa actgaattta gacaatataa gagaacatac tacatgacga agatgggcgt
     1441 cgatgtagta tctgatgagt ttctggctaa tcaggctgcc tgttatgttc aggctataca
     1501 gtggatctta cactattact atcatggagt ccagtcctgg agctggtact atccctatca
     1561 ttacgcaccc ttcctatctg atatccgcag tatcagtaca ctcaaaatcc attttgaact
     1621 cggaaaaccc ttcaagccat tcgagcagct gcttgctgta cttccatcgg ctagtaaaaa
     1681 tttgcttcct acatgctacc agcatttgat gaccagtgaa gactcgccaa ttatagaata
     1741 ttatccacct gatttcaaaa ctgatctaaa tgggaaacaa caggaatggg aagctgtggt
     1801 attaataccg tttattgatg agacgcgatt attggaagcc atggagacat gtaaccactc
     1861 cctcaaaaaa gaagagagga aaagaaacca gcacagtgag tgcctgatgt gctggtatga
     1921 cagagacaca gagttcacct actcctctcc atggccagaa aagttccctg ccatagaacg
     1981 atgttgtaca aggtataaaa tgatatcatt agatgcttgg cgcgtagaca taaacaagaa
     2041 caaaataacc agggttgacc agaaggcatt atatttctgt ggattcccta ctttgaaaca
     2101 catcaaacat aaattttttt tgaagaaaag tggtgttcaa gtattccagc agagtagtcg
     2161 tggtgaaaac ttgatgttgg aaatttcagt aaatgcagaa ccagatgaac ttaggataga
     2221 gaacatagct tcggcggtgc tgggaaaggc agtctttgtt aactggcctc atcttgaaga
     2281 agctagagtt gtggcagtat cagatggtga aactaagttt tacatagaag aacctccagg
     2341 aacacagaag gtttatttgg gaaaaactgc cccaccatct aaagtcatcc aacttacaga
     2401 taaagaacaa tcgaactgga ctaaagaaat acaaggaatt tcagaacaat atctaagaag
     2461 aaagggaata ataataaatg aaacatctgc tgttgtgtat gctcagttgc tcacgggtcg
     2521 taaatatcaa atcagtcaga atggtgaagt tcgtctagaa aaacagtggt caaaacaaat
     2581 tcttcctttt gtttatcaaa ctattgttaa ggacatccga gcttttgact ctcgtttctc
     2641 caatatcaag acattggatg acttgtttcc accgagaact atggtcttta tgcttggaac
     2701 tccttattat ggctgcactg gagaagttca ggattcagga gacttgatta cagaagggag
     2761 aattcgtgtg gttttcagca ttccatgtga gccaaatctt gatgctttaa tacagaatca
     2821 gcataaatac tctataaagt acaacccagg atatgtgttg gccggtcgcc ttggcgtgag
     2881 tggatacctt gtttcaaggt ttacaggaag tatttttatt ggaagaggat ctaggagaaa
     2941 ccctcatgga gaccataaag caaatgtggg tttaaatctc aaattcaaca agaaaaatga
     3001 ggaagtccct ggctatacaa agaaagtggg gaacgagtgg atgtactcgt cggcagcaga
     3061 gcagctcctc gcagagtaca tagagagagc accagaactg ttcagttata tagcaaaaaa
     3121 cagccaagaa gatgtattct atgaagatga catctggcct ggagaaaatg agaacggggc
     3181 tgagaaagtt caagaaatta ttacttggct aaaaggacat cctgtcagta ctctgtctcg
     3241 gtcttcttgt gacttgcaca ttctggatgc agccattgtt gaaaaaatcg aggaagaagt
     3301 tgagaagtgc aagcaaagaa agagcaacaa gaaagtccga gtaacagtga agccccattt
     3361 gctgtacaga cccttagagc agcagcatgg agtcattcct gatcgggatg ccgagttccg
     3421 gctctttgat cgcgttgtga acgtgagaga gagcttctcc gtgccagtcg gccttcgagg
     3481 caccgtcatt ggaattaaag gggctagcag agaagctgat gtactatttg aagtattatt
     3541 tgatgaggaa tttcccggag gcttaacaat aaggtgctca cctggtaggg gataccgact
     3601 gccaacaagt gccttggtga acctttctca tgggagtcgg tgtgaaactg gaaaccagaa
     3661 gttgacagcc attgtgaaac cacaaccttc tgtgagtcac tgtagtgcag ctccctccgg
     3721 gcacttggga ggcctcaacc actctcctca gtcacctttt cttcctactc aggtacctac
     3781 aaaaggtgat gatgaatttt gcaacatttg gcaatctcta cagggagctg gaaaaattca
     3841 acacttgcag ccgacagtac aagagaaggg tgcagttcta cctcaagaaa taagtcaagt
     3901 aactgaaggt cataaatctg gctttactga ccacagtgtc agacatcagc agagaaaaca
     3961 tgactcgcaa cgaaaattta aggaagaata taaaagtcct aaagctgagt gtcagagtca
     4021 aaagttgtcc agtaaacaga cttctggagg gtctgccaga tgtagtatta aactattgaa
     4081 gagaaatgaa agtccaggaa cttcagaagc ccagaaggtt gtaactagtt acccaaatgc
     4141 agtgcataag cctccctctg ggattgagaa ctttttagca tctttgaatc tctctaagga
     4201 gaatgaagca cagctacctc atcatggaga gcctccagat gaggcggatt tgtccccgca
     4261 gtcctttgct atgaaaggaa cacggatgct taaagaaatt ctgaaaattg atagccctga
     4321 cacaagagac agcaagaatg atatgaagaa atctgataat gaagccactg tttcttctag
     4381 aagagatgag cgtggagtgt cagcacatcc taagccaaca tgccatatga acaagcctca
     4441 tgggactaac gagttccaaa atgtggcatc ggtggacagt gtgtgttggc caggccagat
     4501 gcctcctgtg tccacaccag taactgaact ttctcgaatt tgttcccttg ttggaatgcc
     4561 acaacctgat ttctcctttc tgcgaacaac acagacaatg acagtttgtc aggtaaaatt
     4621 gtctaatggc ttactggtac atgggccaca gtgccactct gaaagtgaag ccaaggagag
     4681 agctgctctt tttgctttac agcaactggg ctccttgggt gtgagttttc ctttgcctcc
     4741 acccatattt acaaattatc ctcctgcggt accacctgga gccgtccctc ctgtttttac
     4801 ccaacctact gctaatataa tgccttcatc gtctcatctc tttggctcag tgtcatggag
     4861 accaccagtg ccagttgctg ggaatgcctt ccactatcct tcatatcctg gaaccatgcc
     4921 cttggctgga ggagtgccag gcggagtgca cagtcagttc atacctctgc aggttaccaa
     4981 aaaaagagtt gcaaacagaa agaattttga gaataaggag gcccagagtt ctcaggccac
     5041 accacttcag actaacaagc cagggtcttc tgaagccacc aaaatgactc cacaagaaag
     5101 tccaccagcc tcttcatcat cctctcaggc tgcccagccc gtgtcttctc atgttgaaac
     5161 tgcctcccaa ggccatgttg ggtcccaacc aaggtcagcg ccaagctcct caaaaaggaa
     5221 gtcgagaaaa ctggccgtca acttcagcgt ttctaaacct tcggaataaa tttggtacct
     5281 gggaattcat ttcttttatc tccatctact tttttataaa ctgatatcta tgtgtcagaa
     5341 atgtacaatt ttagggtatg tttagattaa atatttttta tttttaagtt accaggcact
     5401 tttcatgcta tttaagacta tatatcaaat tgtgcacttt aaatatgtgc aatttgttgt
     5461 acatcagtga tacctcaata aatctgtaaa aaaagaaaaa ttaaaccttg tattaaaata
     5521 aatagcaaac tgagcatggt aataacctat gagcatcatg ctttagaatc tgagacagaa
     5581 gggttgccat atattcactg tgtgggacta catagtgagt tcctggccag accttgcttc
     5641 aaaaaccaaa aactacagta aatatgagtg gtctgaatgt taaaatgtac cactctaacc
     5701 attggcttcc tcattagaag gatccttgaa ctataaatta gtcattatct tatcatcata
     5761 acaagctttt ttacactatt attgagggat aactaaatgc ggcatgaatt aggccttgta
     5821 atttcctaag tgtgggtttt atttgtttgt aatgatggaa caacatgact ggagagtggc
     5881 ctgcactctg ttcacagccg tctgtggcca aaatcagtcc ctcggatgct ctggagcgag
     5941 cacgcacacc gcacaccggc ggtggccatg gagctattga gcgctgatgc ggccagtgct
     6001 gctttgttct aaatcttgta ccaaagcaac tttagaagat tctccagagc attcgtctga
     6061 ctgcttcaaa ctaggaatag caatctagat ttgaaaaata acgaattttt ttagattatt
     6121 caaaaaccag cattcagaat tcctaataga attaagtttc aaaatctcca gggtacagcg
     6181 attacaaatc agactgcaat agagtacagt acagaacgtt aatcactatg cttttttagg
     6241 cttcttgggt tatatttgct ttgttgatat ttttttttct tttgagacgg ggtcttgctg
     6301 tgtagtcctg gctggccggg tactcatggt aatcctccat gttctgagat tccagcatgc
     6361 gtcaccacac ttccttatgt ggctttttct ctgcttactt cacatatgtg aaaactcgtt
     6421 gtgggtctgt gtgtagagct tcctgggact ggtaaaaatg taaacttttt aacaattcca
     6481 acatgtcttt ttgtgtggac attttttact gcctgtccca ttctggttgt gcattttagc
     6541 acctgctgaa ctgctcagtg ctgttgcatt ttgtctctgc gtgtcactcc tggatgtgtg
     6601 ttgctcacac tgttgaaatg atttggaatt tatgaaattt tctcaaggac atgatggcgt
     6661 gcatttatat ataaacatat tgatctttta gttttgtgat atgcttatat ttttaaagag
     6721 taaaaatgat cgtacttttt caaaagtagg aaccagtctt tatatggagc tgctcattgt
     6781 gtgtatttaa tttacgtcat ggagactctg ccataagtct taaaatagtc ttactgtttc
     6841 ttacaaatgc tggtgttgaa ttcctttctg tgctaatttc agtgtttctc taggtgatgg
     6901 caggatatat ggatactagt agtgccttta gttaagaggt aaatgatagc tatctttatt
     6961 ttctatggtc atgtgaattt ctgtaacttt gtcttctgtt agtagaaact tcttgctggg
     7021 gcactgtggg aaggttcagg ccattcgcag tgtttgcctt tgttgctccc aaactctgtt
     7081 ttcaagggac gcatccattg cagacgcgag ggaagttagg ctgtggcgat gctatggtaa
     7141 ctccttccga gagcgttgaa atgtgtagta tttgttacct cacttacgtt tgctgtaccc
     7201 tcgctcgctc ctgtgggata aattcaccgt gctgcacaac catgtcccct gtcgaaacag
     7261 aggaaggagt tgttctgaca attccatgag aaatttgagt aaacacagtg ccattgaacc
     7321 agtgaggatt ctccaaaaac tgtgcacaac aagctagtaa ttctgcatgg aaagcagagt
     7381 aagaagagcc tttgccttcc ttttaaaaat agagagcaaa tgctaagagt caagcttgca
     7441 aagaaaagga gaggattccg tcctggcagc tagggagcag cagcagagct ctgaggacac
     7501 tggatctcct gacacgtgtc tgcgggggag gagcacatct ctgaaacata catgggctgt
     7561 ggcaaccata tagccagttg ggatgagtca gtctgaaacc accctcgagc ttttaaaata
     7621 tgttcatttg gctattcaat ctaagaacct ttggaaatgg tgggctgtgg tcccagaagg
     7681 aacacttccc ccagattgtc tgtttccgtg taactagaaa agttatttat ttgattaagc
     7741 cttcctctcc tcactggaaa tgattgttaa tatctttgtt caacaagtct ttctttgtta
     7801 agcaaattaa gttcattaga agatgggtcc tttcagctgt gtctcccttt ctcaattttg
     7861 tacatttcct ggccaatgga ttcattgacc taagaacttc atagactaaa ggcttaaata
     7921 tctaaacctc agttgtgtca aaactttatt ccctctaaac ctcagtttaa attacagatt
     7981 ttccttctct gggctcagta gtgcacgtct ccagttccag tgctctggaa gctgtctata
     8041 gcaggaggtt ccgtgaattc aaggcagcca ggcagtaatg aagtctgaag gtacttcccg
     8101 ccttctgtga tgagcagaag gaaggtgttg ctgcctctca acgtgtactc cattcccaca
     8161 ccgagagtcc cgtgctcact ttgtgactag agagttctgg agtcttggct ctaggggagg
     8221 aggcgaagag acaggtgaga tgcagtgctg tttgcagagt gtaaggtact gacttagctt
     8281 aaggaaatga actagagagt catcttattt gctctctttt tacgtaacta ttctttttga
     8341 atcaacaaat acttttcata gtggttatct agttcccacc ttatatagca gaactccaca
     8401 gaaggtagcg ctcacacaga tcttctcgga taaatgttct ttgtgctatc ttgcttccag
     8461 atttttcttt tattatagaa gagaatctgt tagaaattac tgatagctgg gcatggtgga
     8521 gcacaccttt gatcccagca ctcgggaggc agaggcaggt ggatttctga gttcaaggcc
     8581 agcctggtct acaaagtgag ttcaaggaca gccagagcta cacagagaaa ccctgtctca
     8641 aaaaaaaaaa aaaattacta tgaaaggcat ccggtatctt tttttttttt taaatcgatc
     8701 actaaaaaaa aaaaaaaaag aaaagaatca ttatttttta gaaatagtga caggtctcaa
     8761 agtgcagctg tttgtgtgca ttttatgttg tggcagtgtt gttgttttca acaaagtgca
     8821 gacaccccct ttgctagaag aaaacaaagc ttaagttttt agtgtttttc agactgactt
     8881 aatttatatt cctgttagac atcctcgctt tgtgccaggg cacagtaaga aacagctgga
     8941 gaggggcagc agctctccag caatggggaa aagcagacag gcagacccat gtttaccatc
     9001 acacatcatg tgcgttagct catacagcac atgtccttct ttctgagaga ggatagaaga
     9061 aaatagttga agaatgttga gaatctgtgt gtctgccttc gggtggaaac tccctgctct
     9121 gcagagactg agctcaagta cagctggtga aggagactgc tttccaggtt tcattttggc
     9181 acagtgaaac ctgtagatgt gaattcccac caccaccacc accacccctc acccccagtt
     9241 ggctcattac tgccaatgtt gcaaaaggta atggtctgtg taactgcatt agaagccaag
     9301 tacttgttct gtttctctcg aaatcatggg ggtgaaaatt ttctctgaag cctgctgtaa
     9361 gtccttataa ctcttgttca ctcgggcttt ctctttcttc ccccttctcc aattcctctt
     9421 gctatctagc ccttcagtcc tcctgcctcc acctcctggg ttttgaggtt acagagtttg
     9481 gccccaggcc cagtgtcctt taagctcatt aatcctgcct ttcctttaca gttatgggtg
     9541 tgtctatcta tgtgtgtgtg tgtgtgtgtg tgttggactt ttgctttgtt tttcaaagca
     9601 gggtttctct gtatagccct ggctgtcctg gaactcactc tgtagaccag gctggcctcg
     9661 aactctcaga gatcctcctg cttctgcctc ccaagtgtta gaattaaagg tgtgtgccac
     9721 cactgcccag ctggatttta tattttagct ttttttcttg gtctcaagaa tcttacattt
     9781 tcttttgctg cacaaattac tttgagtgtt ttcacttgac tctgagtaat gggtggtatg
     9841 ctgtatatca aagtgtttta acatttgctt cattttaata gtgtgatttc atatatatct
     9901 atcttccttg attcttttgt catgtaacta ttttgtaact aactggaatt tctgattaga
     9961 tcttagacag agcagtattt tattaaaaat tccaaaggga agacttcttt gtgctcattt
    10021 tgtaattttt attttaaata tctttacatt gtatgtctat tgcatttaaa gcttttaaac
    10081 ttacatggaa tggactccaa gtgtattttt atgttgtaca tttgtaaatt catagcacga
    10141 aattagcctc aaaatgttat gcaaagggat cttgacatgc aaaagtcact gaaggtctgg
    10201 acacttgtat gtgtcaaatg cgctatgatt ttgattaaag atggaatgac gacgacggta
    10261 cgacac
//
