LOCUS       NM_001411249            2271 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus X-ray repair complementing defective repair in Chinese
            hamster cells 6 (Xrcc6), transcript variant 2, mRNA.
ACCESSION   NM_001411249 XM_006520443
VERSION     NM_001411249.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Pandey,A., Shen,C., Feng,S., Enosi Tuipulotu,D., Ngo,C., Liu,C.,
            Kurera,M., Mathur,A., Venkataraman,S., Zhang,J., Talaulikar,D.,
            Song,R., Wong,J.J., Teoh,N., Kaakoush,N.O. and Man,S.M.
  TITLE     Ku70 senses cytosolic DNA and assembles a tumor-suppressive
            signalosome
  JOURNAL   Sci Adv 10 (4), eadh3409 (2024)
   PUBMED   38277448
  REMARK    GeneRIF: Ku70 senses cytosolic DNA and assembles a
            tumor-suppressive signalosome.
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Myler,L.R., Toia,B., Vaughan,C.K., Takai,K., Matei,A.M., Wu,P.,
            Paull,T.T., de Lange,T. and Lottersberger,F.
  TITLE     DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at
            leading-end telomeres
  JOURNAL   Nat Struct Mol Biol 30 (9), 1346-1356 (2023)
   PUBMED   37653239
REFERENCE   3  (bases 1 to 2271)
  AUTHORS   Wang,Y., Liu,B., Lu,H., Liu,J., Romanienko,P.J., Montelione,G.T.
            and Shen,Z.
  TITLE     SETD4-mediated KU70 methylation suppresses apoptosis
  JOURNAL   Cell Rep 39 (6), 110794 (2022)
   PUBMED   35545041
  REMARK    GeneRIF: SETD4-mediated KU70 methylation suppresses apoptosis.
REFERENCE   4  (bases 1 to 2271)
  AUTHORS   Saha,J., Bae,J., Wang,S.Y., Lu,H., Chappell,L.J., Gopal,P. and
            Davis,A.J.
  TITLE     Ablating putative Ku70 phosphorylation sites results in defective
            DNA damage repair and spontaneous induction of hepatocellular
            carcinoma
  JOURNAL   Nucleic Acids Res 49 (17), 9836-9850 (2021)
   PUBMED   34428289
  REMARK    GeneRIF: Ablating putative Ku70 phosphorylation sites results in
            defective DNA damage repair and spontaneous induction of
            hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 2271)
  AUTHORS   Tsuchiya,H., Shimada,M., Tsukada,K., Meng,Q., Kobayashi,J. and
            Matsumoto,Y.
  TITLE     Diminished or inversed dose-rate effect on clonogenic ability in
            Ku-deficient rodent cells
  JOURNAL   J Radiat Res 62 (2), 198-205 (2021)
   PUBMED   33372229
  REMARK    GeneRIF: Diminished or inversed dose-rate effect on clonogenic
            ability in Ku-deficient rodent cells.
REFERENCE   6  (bases 1 to 2271)
  AUTHORS   Paesen,G.C., Zanotto,P.M. and Nuttall,P.A.
  TITLE     A tick homologue of the human DNA helicase II 70-kDa subunit
  JOURNAL   Biochim Biophys Acta 1305 (3), 120-124 (1996)
   PUBMED   8597595
  REMARK    Erratum:[Biochim Biophys Acta 1996 Jul 17;1307(3):349]
REFERENCE   7  (bases 1 to 2271)
  AUTHORS   Thompson,L.H. and Jeggo,P.A.
  TITLE     Nomenclature of human genes involved in ionizing radiation
            sensitivity
  JOURNAL   Mutat Res 337 (2), 131-134 (1995)
   PUBMED   7565861
REFERENCE   8  (bases 1 to 2271)
  AUTHORS   Falzon,M. and Kuff,E.L.
  TITLE     The nucleotide sequence of a mouse cDNA encoding the 80 kDa subunit
            of the Ku (p70/p80) autoantigen
  JOURNAL   Nucleic Acids Res 20 (14), 3784 (1992)
   PUBMED   1641347
REFERENCE   9  (bases 1 to 2271)
  AUTHORS   Baughman,G., Harrigan,M.T., Campbell,N.F., Nurrish,S.J. and
            Bourgeois,S.
  TITLE     Genes newly identified as regulated by glucocorticoids in murine
            thymocytes
  JOURNAL   Mol Endocrinol 5 (5), 637-644 (1991)
   PUBMED   2072923
REFERENCE   10 (bases 1 to 2271)
  AUTHORS   Porges,A.J., Ng,T. and Reeves,W.H.
  TITLE     Antigenic determinants of the Ku (p70/p80) autoantigen are poorly
            conserved between species
  JOURNAL   J Immunol 145 (12), 4222-4228 (1990)
   PUBMED   1701785
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102176.8.
            
            On Aug 23, 2022 this sequence version replaced XM_006520443.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422598.1616822.1,
                                           SRR17253014.132581.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-264               AC102176.8         46878-47141
            265-353             AC102176.8         47593-47681
            354-466             AC102176.8         49464-49576
            467-605             AC102176.8         52928-53066
            606-860             AC102176.8         53290-53544
            861-1044            AC102176.8         55988-56171
            1045-1231           AC102176.8         57708-57894
            1232-1400           AC102176.8         59605-59773
            1401-1562           AC102176.8         60046-60207
            1563-1692           AC102176.8         61616-61745
            1693-1793           AC102176.8         66160-66260
            1794-1907           AC102176.8         67940-68053
            1908-2271           AC102176.8         70205-70568
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 38.33 cM"
     gene            1..2271
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /note="X-ray repair complementing defective repair in
                     Chinese hamster cells 6"
                     /db_xref="GeneID:14375"
                     /db_xref="MGI:MGI:95606"
     exon            1..264
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            265..353
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..2104
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /note="isoform 1 is encoded by transcript variant 2;
                     ATP-dependent DNA helicase 2 subunit 1; X-ray repair
                     cross-complementing protein 6; CTC75; CTCBF; DNA repair
                     protein XRCC6; ku autoantigen protein p70 homolog; CTC
                     box-binding factor 75 kDa subunit; ATP-dependent DNA
                     helicase II 70 kDa subunit; 5'-dRP/AP lyase Ku70;
                     5'-deoxyribose-5-phosphate lyase Ku70; thyroid autoantigen
                     70 kDa; Ku p70"
                     /codon_start=1
                     /product="X-ray repair cross-complementing protein 6
                     isoform 1"
                     /protein_id="NP_001398178.1"
                     /db_xref="GeneID:14375"
                     /db_xref="MGI:MGI:95606"
                     /translation="MSEWESYYKTEGEEEEEEEESPDTGGEYKYSGRDSLIFLVDASR
                     AMFESQGEDELTPFDMSIQCIQSVYTSKIISSDRDLLAVVFYGTEKDKNSVNFKNIYV
                     LQDLDNPGAKRVLELDQFKGQQGKKHFRDTVGHGSDYSLSEVLWVCANLFSDVQLKMS
                     HKRIMLFTNEDDPHGRDSAKASRARTKASDLRDTGIFLDLMHLKKPGGFDVSVFYRDI
                     ITTAEDEDLGVHFEESSKLEDLLRKVRAKETKKRVLSRLKFKLGEDVVLMVGIYNLVQ
                     KANKPFPVRLYRETNEPVKTKTRTFNVNTGSLLLPSDTKRSLTYGTRQIVLEKEETEE
                     LKRFDEPGLILMGFKPTVMLKKQHYLRPSLFVYPEESLVSGSSTLFSALLTKCVEKEV
                     IAVCRYTPRKNVSPYFVALVPQEEELDDQNIQVTPGGFQLVFLPYADDKRKVPFTEKV
                     TANQEQIDKMKAIVQKLRFTYRSDSFENPVLQQHFRNLEALALDMMESEQVVDLTLPK
                     VEAIKKRLGSLADEFKELVYPPGYNPEGKVAKRKQDDEGSTSKKPKVELSEEELKAHF
                     RKGTLGKLTVPTLKDICKAHGLKSGPKKQELLDALIRHLEKN"
     exon            354..466
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            467..605
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            606..860
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1044
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1231
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1400
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1562
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1692
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1793
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1907
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2271
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2252..2257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /note="hexamer: AATAAA"
     polyA_site      2271
                     /gene="Xrcc6"
                     /gene_synonym="70kDa; G22p1; Ku70"
                     /note="major polyA site"
ORIGIN      
        1 gattgggcga gagacaagga gcccgcgtgg acgtggtgcc caccttctgc tgtcccagtg
       61 gcttgcccgc ctgcggttaa ggtaggttcg ccgcaccaca tgtgttggat gaggcctgcc
      121 ttggtggctt gcttgcacgc ttagtgtcgc gcgacccctg gcctcaccac ggagcagcgc
      181 tgtcgacctg caggacctgg gtcatgctcc tttctcccca gaaggcggaa caggactggt
      241 ggttgagcca atctctagct gaagtgagca aaccaacatg tcagagtggg agtcctacta
      301 caaaactgag ggcgaggaag aggaagagga ggaggagagc cccgacacag gtggagaata
      361 taaatattca ggaagagata gcttgatttt tctggttgat gcctccaggg ctatgttcga
      421 atctcagggt gaagatgaac tcacaccttt tgatatgagc atccagtgta tccagagtgt
      481 gtacaccagt aagatcataa gcagcgatcg ggatctcctg gcagtggtgt tctatggaac
      541 cgagaaagat aaaaattcag tgaatttcaa aaatatttat gtcttacaag atttggacaa
      601 cccaggcgct aagcgagtgc tagagctcga ccagtttaag ggacaacagg ggaagaagca
      661 cttccgagac acggttggcc atgggtctga ctactctttg agtgaagtgc tctgggtctg
      721 tgccaacctc ttcagcgacg tccagctcaa gatgagtcac aagaggatca tgctgttcac
      781 caatgaagac gacccccatg gccgtgacag tgctaaagcc agccgggcca ggaccaaagc
      841 cagcgacctc cgggacactg ggatcttcct tgacttgatg catctgaaga agccaggagg
      901 ctttgatgta tccgtgttct acagggacat catcaccacc gctgaggacg aggaccttgg
      961 ggttcacttc gaggagtcaa gcaagctgga agacctgcta aggaaggttc gagccaagga
     1021 gaccaaaaag cgagttctgt ccaggttaaa gtttaagctc ggtgaagacg tagtactcat
     1081 ggtgggcatt tataacttgg tccagaaagc taacaagcct tttccagtga gactctatcg
     1141 ggaaacaaat gaaccagtga aaaccaagac aaggactttt aatgtaaaca ccggcagtct
     1201 actcctgcct agtgacacca agcggtctct gacttatggg acacgtcaga ttgtgctgga
     1261 gaaagaggag acagaggagc tgaagcggtt tgatgagcca ggtttgatcc tcatgggctt
     1321 taagcccacg gtgatgctga agaagcagca ctacctgagg ccctctctgt tcgtgtaccc
     1381 ggaggagtcc ctggtcagtg ggagctcaac cttgttcagc gctctgctca ccaagtgtgt
     1441 ggagaaggag gtcatagcag tgtgtagata cacaccccgg aagaacgtct ccccgtattt
     1501 tgtggctttg gtgccccagg aagaggagct ggatgatcag aacattcagg tgactccagg
     1561 aggcttccag cttgtcttcc tcccttatgc cgatgacaag cggaaggtgc cctttactga
     1621 gaaggtgacg gccaaccagg agcagataga caagatgaag gccattgttc aaaagctccg
     1681 cttcacatac aggagcgaca gttttgagaa tccagtcctg cagcagcact tccgcaacct
     1741 ggaggcccta gctttggaca tgatggagtc ggagcaagtg gtagatctga cactacccaa
     1801 ggttgaagcc ataaagaaaa gactgggttc cctggcagat gagtttaaag aacttgtcta
     1861 tcctccaggt tataatcccg agggaaaagt tgccaagaga aaacaagatg atgaaggttc
     1921 tacgagtaaa aagcccaagg tagagttatc agaagaagag ctgaaggccc attttcgtaa
     1981 gggcacactg ggtaagctca ctgtacctac actgaaggac atatgcaagg ctcatgggct
     2041 taagagtggg ccgaagaagc aggaactgct agatgctctt atcagacact tggagaagaa
     2101 ctgactaaag ctcagaagcc cagccacttt ctacactgca gccaggccac ctgcttgtgt
     2161 tcttaccaag ctaggtggta gctacaaaga gctacttaag tgcaaaatca tggagattac
     2221 agtctcccac ctcactgtgc cttactctgt gaataaagag ctctaacttt a
//
