LOCUS       NM_009533               2571 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus X-ray repair complementing defective repair in Chinese
            hamster cells 5 (Xrcc5), transcript variant 1, mRNA.
ACCESSION   NM_009533
VERSION     NM_009533.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Zapater,L.J., Lewis,S.A., Gutierrez,R.L., Yamada,M.,
            Rodriguez-Fos,E., Planas-Felix,M., Cameron,D., Demarest,P.,
            Nabila,A., Mueller,H., Zhao,J., Bergin,P., Reed,C.,
            Chwat-Edelstein,T., Pagnozzi,A., Nava,C., Bourel-Ponchel,E.,
            Cornejo,P., Dursun,A., Ozgul,R.K., Akar,H.T., Maroofian,R.,
            Houlden,H., Cheema,H.A., Anjum,M.N., Zifarelli,G., Essid,M., Ben
            Hafsa,M., Benrhouma,H., Montoya,C.I.G., Proekt,A., Zhao,X.,
            Socci,N.D., Hayes,M., Bigot,Y., Rabadan,R., Torrents,D.,
            Kleinmann,C.L., Kruer,M.C., Toth,M. and Kentsis,A.
  TITLE     A transposase-derived gene required for human brain development
  JOURNAL   bioRxiv (2024)
   PUBMED   37163102
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Ratnayaka-Gamage,N.D., Alesi,L.R., Zerafa,N., Stringer,J.M. and
            Hutt,K.J.
  TITLE     Xrcc5/KU80 is not required for the survival or activation of
            prophase-arrested oocytes in primordial follicles
  JOURNAL   Front Endocrinol (Lausanne) 14, 1268009 (2023)
   PUBMED   37900135
  REMARK    GeneRIF: Xrcc5/KU80 is not required for the survival or activation
            of prophase-arrested oocytes in primordial follicles.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2571)
  AUTHORS   Cai,X., Stringer,J.M., Zerafa,N., Carroll,J. and Hutt,K.J.
  TITLE     Xrcc5/Ku80 is required for the repair of DNA damage in fully grown
            meiotically arrested mammalian oocytes
  JOURNAL   Cell Death Dis 14 (7), 397 (2023)
   PUBMED   37407587
  REMARK    GeneRIF: Xrcc5/Ku80 is required for the repair of DNA damage in
            fully grown meiotically arrested mammalian oocytes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2571)
  AUTHORS   Kim,J.H., Park,S.Y., Jeon,S.E., Choi,J.H., Lee,C.J., Jang,T.Y.,
            Yun,H.J., Lee,Y., Kim,P., Cho,S.H., Lee,J.S. and Nam,J.S.
  TITLE     DCLK1 promotes colorectal cancer stemness and aggressiveness via
            the XRCC5/COX2 axis
  JOURNAL   Theranostics 12 (12), 5258-5271 (2022)
   PUBMED   35910805
  REMARK    GeneRIF: DCLK1 promotes colorectal cancer stemness and
            aggressiveness via the XRCC5/COX2 axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2571)
  AUTHORS   Muller,B., Serafin,F., Laucke,L.L., Rheinhard,W., Wimmer,T. and
            Stieger,K.
  TITLE     Characterization of Double-Strand Break Repair Protein Ku80
            Location Within the Murine Retina
  JOURNAL   Invest Ophthalmol Vis Sci 63 (6), 22 (2022)
   PUBMED   35737378
  REMARK    GeneRIF: Characterization of Double-Strand Break Repair Protein
            Ku80 Location Within the Murine Retina.
REFERENCE   6  (bases 1 to 2571)
  AUTHORS   Nussenzweig,A., Chen,C., da Costa Soares,V., Sanchez,M., Sokol,K.,
            Nussenzweig,M.C. and Li,G.C.
  TITLE     Requirement for Ku80 in growth and immunoglobulin V(D)J
            recombination
  JOURNAL   Nature 382 (6591), 551-555 (1996)
   PUBMED   8700231
REFERENCE   7  (bases 1 to 2571)
  AUTHORS   He,D.M., Lee,S.E. and Hendrickson,E.A.
  TITLE     Restoration of X-ray and etoposide resistance, Ku-end binding
            activity and V(D) J recombination to the Chinese hamster sxi-3
            mutant by a hamster Ku86 cDNA
  JOURNAL   Mutat Res 363 (1), 43-56 (1996)
   PUBMED   8632777
REFERENCE   8  (bases 1 to 2571)
  AUTHORS   Chen,D.J., Park,M.S., Campbell,E., Oshimura,M., Liu,P., Zhao,Y.,
            White,B.F. and Siciliano,M.J.
  TITLE     Assignment of a human DNA double-strand break repair gene (XRCC5)
            to chromosome 2
  JOURNAL   Genomics 13 (4), 1088-1094 (1992)
   PUBMED   1505945
REFERENCE   9  (bases 1 to 2571)
  AUTHORS   Falzon,M. and Kuff,E.L.
  TITLE     The nucleotide sequence of a mouse cDNA encoding the 80 kDa subunit
            of the Ku (p70/p80) autoantigen
  JOURNAL   Nucleic Acids Res 20 (14), 3784 (1992)
   PUBMED   1641347
REFERENCE   10 (bases 1 to 2571)
  AUTHORS   Porges,A.J., Ng,T. and Reeves,W.H.
  TITLE     Antigenic determinants of the Ku (p70/p80) autoantigen are poorly
            conserved between species
  JOURNAL   J Immunol 145 (12), 4222-4228 (1990)
   PUBMED   1701785
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK081633.1, AK165470.1 and CJ220465.1.
            
            On Nov 15, 2007 this sequence version replaced NM_009533.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029218.1, AK089944.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1366              AK081633.1         2-1367
            1367-2553           AK165470.1         1335-2521
            2554-2571           CJ220465.1         381-398
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 36.5 cM"
     gene            1..2571
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /note="X-ray repair complementing defective repair in
                     Chinese hamster cells 5"
                     /db_xref="GeneID:22596"
                     /db_xref="MGI:MGI:104517"
     exon            1..147
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..2325
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATP-dependent DNA helicase 2 subunit 2; X-ray repair
                     cross-complementing protein 5; nuclear factor IV; DNA
                     repair protein XRCC5; ku autoantigen protein p86 homolog;
                     CTC box-binding factor 85 kDa subunit; ATP-dependent DNA
                     helicase II 80 kDa subunit"
                     /codon_start=1
                     /product="X-ray repair cross-complementing protein 5
                     isoform 1"
                     /protein_id="NP_033559.2"
                     /db_xref="CCDS:CCDS35608.1"
                     /db_xref="GeneID:22596"
                     /db_xref="MGI:MGI:104517"
                     /translation="MAWSGNKAAVVLCVDVGVAMGNSFPGEESPIEQAKKVMTMFVQR
                     QVFSESKDEIALVLYGTDGTDNALAGKDQYQNITVCRHLMLPDFDLLEDIGNKIQPSS
                     QQADFLDALIVCMDLIQRETIGKKFGKKHIEVFTDLSSPFSQDQLDVIICNLKKSGIS
                     LQFFLPFPIDKNGEPGERGDLDSGLDHLKPSFPQKGLTEQQKEGIRMVTRVMLSLEGE
                     DGLDEIYSFSESLRQLCVFKKIERRSMPWPCQLTIGPNLSIKIVAYKSIVQEKFKKSW
                     VVVDARTLKKEDIQKETVYCLNDDDETEVSKEDTIQGYRYGSDIIPFSKVDEEQMKYK
                     SEGKCFSVLGFCKSSQVHRRFFMGHQVLKVFAAKDDEAAAVALSSLVHALDELNMVAI
                     VRYAYDKRSNPQVGVAFPYIKDAYECLVYVQLPFMEDLRQYMFSSLKNNKKCTPTEAQ
                     LSAIDDLIDSMSLVKKNEEEDIVEDLFPTSKIPNPEFQRLYQCLLHRALHLQERLPPI
                     QQHILNMLDPPTEMKAKCESPLSKVKTLFPLTEVIKKKNQVTAQDVFQDNHEEGPAAK
                     KYKTEKEEDHISISSLAEGNITKVGSVNPVENFRFLVRQKIASFEEASLQLISHIEQF
                     LDTNETLYFMKSMDCIKAFREEAIQFSEEQRFNSFLEALREKVEIKQLNHFWEIVVQD
                     GVTLITKDEGPGSSITAEEATKFLAPKDKAKEDTTGPEEAGDVDDLLDMI"
     exon            148..261
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            262..445
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            446..494
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            495..617
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            618..809
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            810..924
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1063
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1176
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1239
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1377
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1468
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1602
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1799
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1893
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1963
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2073
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2170
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2238
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2310
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2556
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2523..2528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /note="hexamer: TATAAA"
     polyA_site      2556
                     /gene="Xrcc5"
                     /gene_synonym="CTC85; CTCBF; Ku80; Ku86; Kup80"
                     /note="major polyA site"
ORIGIN      
        1 atgctcagag cccatggctt ccccgccttg cacgcgggac gtctggatct gtgggttgcc
       61 cggaactgag ttgcgacact ctaggtttcc gcccggaaaa agtaatcaaa tcacctgagg
      121 accagcatgg cgtggtccgg taataaggca gctgttgtgc tgtgtgtgga tgtgggggtt
      181 gccatgggta actcctttcc tggtgaagaa tctccaattg aacaagcaaa gaaagtgatg
      241 actatgtttg tccaacgaca ggtattttcg gagagcaaag atgagattgc gttagtcctc
      301 tatggcacag atggcactga taatgccctt gctggcaagg accagtatca gaacatcaca
      361 gtgtgcagac acctgatgct accagatttt gatttgctgg aagacatcgg aaacaaaatc
      421 cagccaagtt cccagcaagc cgacttcctg gacgccctga ttgtgtgcat ggatttgatt
      481 cagcgtgaaa ccatagggaa gaagtttggg aagaagcata ttgaagtgtt cactgacctc
      541 agcagcccgt tcagccagga ccaactggac gttataattt gtaacttgaa gaagtctggc
      601 atctctctgc agttcttttt gccttttcca atcgacaaga atggcgagcc tggcgagaga
      661 ggggacctgg actctggctt ggaccacctc aaaccctcct tccctcaaaa aggacttact
      721 gagcagcaaa aggaaggcat ccgcatggtg acaagggtga tgctgtcctt agaaggcgaa
      781 gacgggctgg atgaaattta ttccttcagt gagagtctac gacaactgtg cgtctttaag
      841 aagattgaga ggcgttccat gccctggccc tgccaactga ccattggccc caacttgtct
      901 ataaagattg tagcctataa atcgattgta caggagaaat ttaagaagag ttgggtagtt
      961 gtggacgcaa gaacgctaaa gaaggaagat atacaaaaag agactgttta ttgcctaaat
     1021 gacgatgatg aaactgaagt ttccaaagag gacactattc aagggtaccg ctacggaagt
     1081 gatatcattc ctttttctaa agtggatgag gaacaaatga aatataaatc tgaggggaag
     1141 tgcttctctg ttttgggatt ttgtaaatct tctcaggttc atagaagatt cttcatggga
     1201 catcaagttc taaaggtctt tgcagcaaag gatgatgagg cggcagctgt tgccctttcc
     1261 tctctcgttc atgctttgga tgagttaaac atggtcgcca tcgtccgata tgcttatgac
     1321 aaaagatcta atcctcaagt tggtgtagcc ttcccttaca ttaaggacgc ctatgagtgt
     1381 ttagtttatg tgcagctgcc tttcatggaa gacttgcggc aatacatgtt ttcctcgctg
     1441 aaaaacaata agaagtgcac tcccacagag gcccagctga gcgctattga tgatctgatt
     1501 gattccatga gcttggtaaa gaaaaacgag gaagaagaca ttgttgaaga tttgtttcca
     1561 acctccaaaa ttccaaatcc tgaatttcag cgattgtacc agtgtctgct gcatagagcc
     1621 ttacatctcc aggagcggct gcccccgatt cagcagcaca ttttgaatat gctggatccc
     1681 cccactgaga tgaaagcaaa atgtgagagt ccactctcta aagtaaagac ccttttccct
     1741 ctcacagaag tcatcaagaa aaagaaccaa gtgactgctc aggacgtttt ccaagacaat
     1801 catgaagagg ggcccgctgc taaaaaatat aaaactgaga aagaagaaga tcacatcagc
     1861 atctccagcc tggcagaagg gaacatcacc aaggttggaa gtgtgaatcc tgttgaaaac
     1921 ttccgtttcc tagtaagaca gaagattgcc agctttgagg aagcgagtct ccagttaata
     1981 agtcacatcg aacaattttt ggataccaat gaaacactgt attttatgaa aagtatggac
     2041 tgcatcaaag ctttccggga ggaggccatt cagttttcag aagaacagcg cttcaacagc
     2101 ttcctggaag cccttcgaga gaaagtggaa attaagcaat taaatcattt ctgggaaatt
     2161 gttgttcagg atggagttac tctgatcacc aaagacgaag gcccaggaag ctctatcaca
     2221 gctgaggaag ccacaaagtt tctggccccc aaagacaaag caaaagaaga tacaacggga
     2281 cctgaagaag ctggtgatgt ggatgattta ctggacatga tatagatcat ggatgtgtgg
     2341 ggaatccaag agtgccagct ctgtgctgct gagagctctg gcagcatccc tgaggccgtg
     2401 caggggatgt gaagctctgg acgtcatcct agcagacaac ttgggaatga gtcactccag
     2461 gtctctacag agtgaccata aggacatata ttacaagaaa taacttagac cctattcaaa
     2521 tttataaaga gccattattt tctgattggt atatcaaaaa aaaaaaaaaa a
//
