LOCUS       NM_133213               3498 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus X-prolyl aminopeptidase (aminopeptidase P) 2,
            membrane-bound (Xpnpep2), transcript variant 1, mRNA.
ACCESSION   NM_133213 NM_178074
VERSION     NM_133213.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   3  (bases 1 to 3498)
  AUTHORS   Sherwood,R.I., Maehr,R., Mazzoni,E.O. and Melton,D.A.
  TITLE     Wnt signaling specifies and patterns intestinal endoderm
  JOURNAL   Mech Dev 128 (7-10), 387-400 (2011)
   PUBMED   21854845
REFERENCE   4  (bases 1 to 3498)
  AUTHORS   Ersahin,C., Szpaderska,A.M. and Simmons,W.H.
  TITLE     Rat and mouse membrane aminopeptidase P: structure analysis and
            tissue distribution
  JOURNAL   Arch Biochem Biophys 417 (2), 131-140 (2003)
   PUBMED   12941294
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672274.7, AK164538.1 and AF428102.1.
            
            On or before Mar 20, 2007 this sequence version replaced
            NM_178074.2, NM_133213.1.
            
            Transcript Variant: This variant (1) uses an alternate splice site
            in the 3' coding region, compared to variant 2. This results in a
            shorter protein (isoform 1), compared to isoform 2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF428102.1, AK078835.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               AL672274.7         84387-84559
            174-2379            AK164538.1         2-2207
            2380-3128           AF428102.1         2231-2979
            3129-3498           AL672274.7         112274-112643
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 25.52 cM"
     gene            1..3498
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /note="X-prolyl aminopeptidase (aminopeptidase P) 2,
                     membrane-bound"
                     /db_xref="GeneID:170745"
                     /db_xref="MGI:MGI:2180001"
     exon            1..470
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     CDS             422..2446
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /EC_number="3.4.11.9"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; membrane-bound aminopeptidase P; membrane-bound
                     APP; X-prolyl aminopeptidase 2; xaa-Pro aminopeptidase 2"
                     /codon_start=1
                     /product="xaa-Pro aminopeptidase 2 isoform 1 precursor"
                     /protein_id="NP_573476.2"
                     /db_xref="CCDS:CCDS30106.1"
                     /db_xref="GeneID:170745"
                     /db_xref="MGI:MGI:2180001"
                     /translation="MAQAYWQCYPWLVLLCACAWSYPEPKYLGREDVRNCSTSPERLP
                     VTAVNTTMRLAALRQQMETWNLSAYIIPDTDAHMSEYIGKPDKRREWISGFTGSAGTA
                     VVTMGKAAVWTDSRYWTQAERQMDCNWELHKEVSISSIVAWILAEVPDGQNVGFDPFL
                     FSVDSWKNYDQGFQDSSRHLLSVTTNLVDVAWGSERPPVPSQPIYALPKEFTGSTWQE
                     KVSAVRSYMEHHAKTPTGVLLSALDETAWLFNLRSSDIPYNPFFYSYALLTNSSIRLF
                     VNKSRFSLETLQYLNTNCTLPMCVQLEDYSQVRDSVKAYASGDVKILIGVSYTTYGVY
                     EVIPKEKLVTDTYSPVMLIKAVKNSKEQALLKSSHVRDAVAVIQYLVWLEKNVPKGTV
                     DEFSGAEYIDELRRNENFSSGPSFETISASGLNAALAHYSPTKELHRKLSSDEMYLVD
                     SGGQYWDGTTDITRTVHWGTPTAFQKEAYTRVLMGNIDLSRLVFPAATSGRVIEAFAR
                     RALWEVGLNYGHGTGHGIGNFLCVHEWPVGFQYNNIAMAKGMFTSIEPGYYHDGEFGI
                     RLEDVALVVEAKTKYPGDYLTFELVSFVPYDRNLIDVRLLSPEQLQYLNRYYQTIREN
                     VGPELQRRQLLEEFAWLEQHTEPLSARAPHIISWTSLWVASALAILSWSS"
     exon            471..544
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            545..655
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            656..719
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            720..824
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            825..911
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1058
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1160
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1242
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1438
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1528
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1638
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1716
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1788
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1849
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1919
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            1920..2024
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2084
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2161
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2251
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..3498
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3156..3161
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /note="hexamer: ATTAAA"
     polyA_site      3189
                     /gene="Xpnpep2"
                     /gene_synonym="9030008G12Rik; mAPP"
                     /note="major polyA site"
ORIGIN      
        1 accagtgcct gatgtgagct aataggaaac agcttgctgg agggtgggga acagaattca
       61 gaaactgcta gggtagagct gctgaagagg ggggctggga gaggctgggt gattgagacc
      121 agctgttgct cgtccatctc agagatcttt ggactctgcc caggggcagg ctcacaccct
      181 acctacccta agccacaaga aaccagcaca ggttgcttgg gcagcccgag gcacactctc
      241 cacggtggct tcccagactt cctgaaacaa aagattacag aaggatcctc atctaacatc
      301 gagacacagc catcaacctt atttgcttct gcccaggaaa ggcctggaag aagaggccac
      361 caaacaaccc aaccctctct ccagtccctt gatctactcg tcgcctgcat ctggagaccc
      421 aatggcccaa gcctactggc agtgctaccc ctggcttgtt ctcctctgtg catgtgcctg
      481 gagctaccca gagcccaagt accttggaag ggaagatgtg agaaactgtt caaccagccc
      541 tgagcgcctg ccagttactg ccgtcaatac aacaatgcgg cttgcagccc tccgccaaca
      601 gatggagacc tggaatctct ctgcctacat catcccagac acggatgcac acatgagtga
      661 gtacattggc aaacctgata agaggcgaga gtggatttca ggcttcacag ggtctgcagg
      721 tactgctgtg gtgaccatgg ggaaagcagc tgtctggacc gatagtcgtt actggactca
      781 ggccgaaagg cagatggact gcaactggga gctgcacaag gaagttagca tttcttccat
      841 tgtcgcctgg atcttagctg aagtccctga tggacagaac gtgggcttcg accccttcct
      901 cttttctgtt gattcctgga aaaattatga tcaaggattc caagactcca gcagacacct
      961 gttatcggtt acaaccaacc ttgttgacgt ggcatggggg tcagagaggc ccccagtgcc
     1021 aagccaaccc atttatgccc tgccgaaaga atttacaggg agcacttggc aggaaaaagt
     1081 atccgctgtc cgaagctata tggagcacca tgccaagact ccaactgggg tccttctatc
     1141 tgcacttgat gagacagcct ggctcttcaa ccttcgtagc agtgacatcc cctataaccc
     1201 cttcttctac tcctatgcac tgctcacgaa ctcctccatc aggttgtttg tcaacaagag
     1261 tcgctttagc cttgagacct tgcagtacct gaatacaaac tgcacattac ccatgtgtgt
     1321 gcaacttgag gactacagtc aagttcgtga cagtgtgaag gcctatgcct caggcgatgt
     1381 gaaaatctta attggggtca gctataccac ctatggggtc tatgaagtga tacccaagga
     1441 gaaactcgtg acagacacct actccccagt gatgttaatc aaggctgtga agaacagcaa
     1501 ggagcaggcc cttttgaaga gcagccacgt gcgggacgct gtggctgtga tccagtactt
     1561 ggtctggttg gagaagaacg tgcccaaagg cacggtggat gagttttctg gggcagaata
     1621 cattgatgag ttacgacgga atgaaaactt ctcctctgga cccagttttg aaaccatctc
     1681 tgctagtggc ctgaatgccg ccctggccca ttacagccca acaaaagagc tgcaccgtaa
     1741 gctgtcctca gatgagatgt acctggtgga ttctgggggg caatactggg atgggaccac
     1801 agatatcacc agaacagtac attggggcac tcctactgcc ttccaaaagg aggcctatac
     1861 tcgagtgcta atgggaaaca tcgatctgtc cagacttgtc tttcctgctg ctacatcagg
     1921 gagagtgata gaagcctttg cccgaagagc cttatgggaa gttgggctca attatggtca
     1981 tgggacaggc catggcattg gcaacttcct ctgtgtgcat gagtggccag tgggattcca
     2041 gtataacaac attgccatgg ccaagggcat gttcacttcc attgaacctg gatactacca
     2101 tgatggggag tttggaatcc gtcttgaaga tgtcgccctt gtggtagaag caaaaaccaa
     2161 gtacccaggg gactacctga cttttgaatt ggtgtccttt gtgccctatg accgaaacct
     2221 catcgatgtc aggctgctgt ccccagagca gctccagtac ctgaaccgct actatcagac
     2281 cattcgtgag aatgtaggcc cagagctgca gcgtcgccag ctgctggagg agtttgcatg
     2341 gttggagcag cacacggagc ccctgtcagc cagggcccct catatcatct cctggacctc
     2401 tctgtgggtg gcctctgccc ttgccatcct cagctggagt agctagaggc ccctgattct
     2461 cctgtggact gttcacttgt gtctggggat tcatttgctt ctgctcagct gtcctctccc
     2521 tatgccatac atgtcccaag ggctactgat ggctcaccac cttgaagcca ctggccagat
     2581 cccagacagt atgttctacc ttctcccaga tgtacatact ttatcatgaa ctcccacccc
     2641 tgggccctgg gatatcagag cctgctacag tgagtccctc tctcccaaag taaaggaccc
     2701 tatagggcaa cacccagagg gctccatcct atccccacct gctggagaag gccagagctt
     2761 ttgccaccca cactgactcc agacttttaa aaggtgcctc tggccactct tacccacagg
     2821 cctacagtag cctgtctgct gtatttggag aagaaagcta ctgggtagtc tggtctcaaa
     2881 ctcccatttg tagtattggg gagaacagat aagttgaagt cagctgtggc ctggaactga
     2941 acacctcagt caataatgtg tacctacctc tccccatctg tgaacccgca gcttctaaac
     3001 taccgctgtg ccccttaaca atgaccacat tgggagtgaa ggctcaggat ttagactatg
     3061 gaatggaggg aatggcaagg tgctctggct atcccaggct cccctggtgc ttggagcagt
     3121 ttaatcttct aaggtagagc cgccatggca gtgctattaa agcctctcat atctttgaaa
     3181 acctcttaac cttgtgtggg cagactctga ttcccccatt ttatagatgg gaaaactgac
     3241 tttcagacag aaggtgtggc ctgcctgaag agactctgca cttggttagg ggacgaggtt
     3301 gggagatgat ggggccagat gggttggcat tctagttcct ttgcccatca gcaccatgcc
     3361 aagcctctta ccctacatct cagcccctgc ctcatttatt gaacccactg tcatcagtgg
     3421 tttctctggt ctggccctgt ctagccgtta gccacctggg ctcacatcct gctagatgct
     3481 taaaacaact ttgcaaag
//
