LOCUS       NM_201368               4238 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus X-linked Kx blood group related 8 (Xkr8), mRNA.
ACCESSION   NM_201368 XM_355525
VERSION     NM_201368.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Neniskyte,U., Kuliesiute,U., Vadisiute,A., Jevdokimenko,K.,
            Coletta,L., Deivasigamani,S., Pamedytyte,D., Daugelaviciene,N.,
            Dabkeviciene,D., Perlas,E., Bali,A., Basilico,B., Gozzi,A.,
            Ragozzino,D. and Gross,C.T.
  TITLE     Phospholipid scramblase Xkr8 is required for developmental axon
            pruning via phosphatidylserine exposure
  JOURNAL   EMBO J 42 (14), e111790 (2023)
   PUBMED   37211968
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Chen,K., Li,C., Dong,C., Cen,X., Wang,Y., Liang,Y., Zhu,Y., Fang,S.
            and Jiang,H.
  TITLE     A dominant variant in apoptosis-related gene XKR8 is relevant to
            hereditary auditory neuropathy
  JOURNAL   J Transl Med 21 (1), 279 (2023)
   PUBMED   37101210
  REMARK    GeneRIF: A dominant variant in apoptosis-related gene XKR8 is
            relevant to hereditary auditory neuropathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4238)
  AUTHORS   Yamashita,Y., Suzuki,C., Uchiyama,Y. and Nagata,S.
  TITLE     Infertility Caused by Inefficient Apoptotic Germ Cell Clearance in
            Xkr8-Deficient Male Mice
  JOURNAL   Mol Cell Biol 40 (3), e00402-19 (2020)
   PUBMED   31712393
  REMARK    GeneRIF: Xkr8 (-/-) male mice were infertile due to reduced sperm
            counts in their epididymides.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4238)
  AUTHORS   Sakuragi,T., Kosako,H. and Nagata,S.
  TITLE     Phosphorylation-mediated activation of mouse Xkr8 scramblase for
            phosphatidylserine exposure
  JOURNAL   Proc Natl Acad Sci U S A 116 (8), 2907-2912 (2019)
   PUBMED   30718401
  REMARK    GeneRIF: These results indicate that PtdSer exposure can be
            increased by the phosphorylation-mediated activation of Xkr8
            scramblase and flippase down-regulation.
REFERENCE   5  (bases 1 to 4238)
  AUTHORS   Kautzman,A.G., Keeley,P.W., Ackley,C.R., Leong,S., Whitney,I.E. and
            Reese,B.E.
  TITLE     Xkr8 Modulates Bipolar Cell Number in the Mouse Retina
  JOURNAL   Front Neurosci 12, 876 (2018)
   PUBMED   30559640
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4238)
  AUTHORS   Kawano,M. and Nagata,S.
  TITLE     Lupus-like autoimmune disease caused by a lack of Xkr8, a
            caspase-dependent phospholipid scramblase
  JOURNAL   Proc Natl Acad Sci U S A 115 (9), 2132-2137 (2018)
   PUBMED   29440417
  REMARK    GeneRIF: These results indicate that the Xkr8-mediated PtdSer
            exposure in apoptotic lymphocytes and aged neutrophils is essential
            for their clearance, and its defect activates the immune system,
            leading to lupus-like autoimmune disease.
REFERENCE   7  (bases 1 to 4238)
  AUTHORS   Kim,G.W., Nam,G.H., Kim,I.S. and Park,S.Y.
  TITLE     Xk-related protein 8 regulates myoblast differentiation and
            survival
  JOURNAL   FEBS J 284 (21), 3575-3588 (2017)
   PUBMED   28881496
  REMARK    GeneRIF: The Xkr8 influences myogenesis by acting as a positive
            regulator of terminal differentiation and myoblast survival.
REFERENCE   8  (bases 1 to 4238)
  AUTHORS   Suzuki,J., Imanishi,E. and Nagata,S.
  TITLE     Exposure of phosphatidylserine by Xk-related protein family members
            during apoptosis
  JOURNAL   J Biol Chem 289 (44), 30257-30267 (2014)
   PUBMED   25231987
  REMARK    GeneRIF: Unlike Xkr8, which is ubiquitously expressed, Xkr4 and
            Xkr9 expression is tissue-specific.
REFERENCE   9  (bases 1 to 4238)
  AUTHORS   Suzuki,J., Denning,D.P., Imanishi,E., Horvitz,H.R. and Nagata,S.
  TITLE     Xk-related protein 8 and CED-8 promote phosphatidylserine exposure
            in apoptotic cells
  JOURNAL   Science 341 (6144), 403-406 (2013)
   PUBMED   23845944
  REMARK    GeneRIF: findings show the Xk-family protein Xkr8 mediates
            phosphatidylserine exposure in response to apoptotic stimuli;
            Xk-family proteins have evolutionarily conserved roles in promoting
            phagocytosis of dying cells by altering the phospholipid
            distribution in the plasma membrane
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK029920.1.
            
            On Jan 26, 2004 this sequence version replaced XM_355525.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029920.1, BC057035.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 65.81 cM"
     gene            1..4238
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /note="X-linked Kx blood group related 8"
                     /db_xref="GeneID:381560"
                     /db_xref="MGI:MGI:2685877"
     exon            1..374
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1287
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /note="X Kell blood group precursor related family member
                     8 homolog"
                     /codon_start=1
                     /product="XK-related protein 8"
                     /protein_id="NP_958756.1"
                     /db_xref="CCDS:CCDS18732.1"
                     /db_xref="GeneID:381560"
                     /db_xref="MGI:MGI:2685877"
                     /translation="MPLSVHHHVALDVVVGLVSILSFLLDLVADLWAVVQYVLLGRYL
                     WAALVLVLLGQASVLLQLFSWLWLTADPTELHHSQLSRPFLALLHLLQLGYLYRCLHG
                     MHQGLSMCYQEMPSECDLAYADFLSLDISMLKLFESFLEATPQLTLVLAIVLQNGQAE
                     YYQWFGISSSFLGISWALLDYHRSLRTCLPSKPRLGRSSSAIYFLWNLLLLGPRICAI
                     ALFSAVFPYYVALHFFSLWLVLLFWIWLQGTNFMPDSKGEWLYRVTMALILYFSWFNV
                     SGGRTRGRAVIHLIFIFSDSVLLVTTSWVTHGTWLPSGISLLMWVTIGGACFFLGLAL
                     RVIYYLWLHPSCSWDPDLVDGTLGLLSPHRPPKLIYNRRATLLAENFFAKAKARAVLT
                     EEVQLNGVL"
     exon            375..571
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /inference="alignment:Splign:2.1.0"
     exon            572..4238
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1817..1822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /note="hexamer: ATTAAA"
     polyA_site      1842
                     /gene="Xkr8"
                     /gene_synonym="4931440N07Rik; Gm1031; mXkr8; XRG8"
                     /note="major polyA site"
ORIGIN      
        1 gacgactgcc ccgccccctt cctgccggac tagcggggcg ggagggcagg tccgcggttg
       61 tgtggttgct tggagaggat catgcctctg tccgtgcacc accatgtggc cttagacgtg
      121 gtcgtaggcc tggtgagtat cttgtctttc ctgctggatc tggtcgctga cctgtgggcc
      181 gttgtccagt acgtgctcct tggccgttat ctgtgggccg cgctggtact ggtcctgctg
      241 ggccaagctt cggtgctgct gcagctcttc agctggctct ggctgacagc tgatcccacc
      301 gagctgcacc attcgcagct ctcgcgtcct ttcctggctc tgctgcacct gctgcagctc
      361 ggctacctgt ataggtgttt gcacggaatg catcaagggc tgtccatgtg ctaccaggag
      421 atgccatccg agtgtgacct ggcctacgca gactttctct ccctggacat cagcatgctg
      481 aagcttttcg agagcttcct ggaggcgacg ccacagctca cactggtgct ggcaattgta
      541 ttgcagaatg gccaggcgga atactaccag tggtttggca tcagctcatc ctttcttggc
      601 atctcgtggg cactgctgga ttaccatcgg tctctgcgta cctgtcttcc ctccaagcca
      661 cgcctgggcc ggagttcctc tgctatctac ttcctgtgga acctgctgct gctggggccc
      721 agaatctgtg ccatcgcctt gttctcagct gtcttcccct actatgtggc cctgcatttc
      781 ttcagcctgt ggctggtact tttgttctgg atctggcttc aaggcacaaa ttttatgcct
      841 gactccaaag gtgagtggct gtaccgggtg acaatggccc tcatcctcta tttctcctgg
      901 ttcaacgtgt ctgggggccg cactcgaggc cgggccgtca tccacctgat cttcatcttc
      961 agtgacagtg ttctgctggt caccacctcc tgggtgacac acggcacctg gctgcccagt
     1021 gggatctcat tgctgatgtg ggtgacaata ggaggagcct gcttcttcct gggactggct
     1081 ttgcgtgtga tctactacct ctggctgcac cctagctgca gctgggaccc tgacctcgtg
     1141 gatgggaccc taggactcct ttctccccat cgtcctccta agctgattta taacaggcgt
     1201 gccaccctgt tagcagagaa cttcttcgcc aaggccaaag ctcgggctgt cctgacagag
     1261 gaggtgcagc tgaatggagt cctctgaggc agggtctgat tcagccagtg aggaagataa
     1321 tgcgagtggg gccttgcaag ggacaaggcg ggccagtcat gtgcaagcca ttttttttct
     1381 tctgaagccg atggaactgc tgtcagcaaa cactcggttg tttgttgttc tcacctctca
     1441 ggtgattggt ggcgtcctgg ctcctggttc cctagcccgc tctagatgac acaagattct
     1501 gggagaactc ttccctaccc catcccatcc attcacttca accaacaaat gctaaaggca
     1561 ctttatgttc tgggaacacc atcctggctt ctgaactgcc tgccactcta gcttctttcc
     1621 ctgcccacct ggacagatcc tgggtagact cctaaacagt gaggccaggt atgtccctcc
     1681 agtgtcctga tgctcaggcc acctttatac caagtgcctt atggacctgt ggtctaggcc
     1741 atgtgatgcc cagtaagtat tttcattctc ctacctcagt ctatgtggaa gaacatatat
     1801 gcatgtgttt aacagtatta aagcctcatg agattctcca gaccagtatg taccactaag
     1861 tgtagtctat caccctttac agacacgtag aaggcgcctg gaacccctta aaactgacac
     1921 agacccctgg catacaaatg tgggcatagg tttgacttaa ttttgcttcc caagacgcag
     1981 gggctagtga gcccgagccg gttgatcatt cggctagcag aactcatggg cagatgctag
     2041 tgtattcttt tagcagctcc gtactgagcc taaagaggac ttgaggatgg ggatggcagg
     2101 tttgaggggc tggatggaag gtaaaggatt gggggttctt tttgggtgag aggtgcagtg
     2161 gcttctggga tgtggtcaat agctccgtgg aggtggcgtg ttctgctctc ggaggtttgt
     2221 ggtcttgttg ggaaaaggga acaggagaga ggctccaggg gcagaagaaa aggttccagg
     2281 tcccagtgct gggacccaga tagttctagc agtcattcat ttatttgtgt ggacgtgaaa
     2341 taacctgtga cccaaacaag caccaagtac tgaaagaaaa ccagatggag aggtgagagg
     2401 gaggatgtat gttgtgggtg gaagttgcag ctttataaaa aaccattggg gaggacccct
     2461 ctgagaaact gaggcataga ctgtaagcta cttcagcagt gactgcagca tggagtctgc
     2521 gtggtttgtt ggagaaggaa tctgcgaatg ctgttccctg tggcacagca accccactgt
     2581 aagaggactg tggggtgcgg ttggctcaca gccaaggagg ctgcagagat gcaggtgggg
     2641 gcctggaaga ggctctggga gaaggtactt cttatactaa aaggtacagg ctgactatgg
     2701 acagaaagga cctaatttcc agacctgaat tttacagacc aggaaaagga gccaaagtgg
     2761 ttgttgatgt taaaagggtc tgaaaaacag tcaccacctc cgtgttcact ctcatggaaa
     2821 aacggatgta atcacaccag aaggtgtcat cctctaaaca gatgccccca caggtacaca
     2881 cctgaaatca ctgttactct catttatgaa aatggtaaga tagggatgag ccagtgtgac
     2941 acacctacca gtctgggcaa ggacatcagg agttcagact cctcagtgac aatgtcagag
     3001 gccagcttgg gctacatgag accctgtctc caacaaaatg aaattatttt atttatttat
     3061 ttatttggct ttttgagacg gggtttctct gtgtagccct ggctgtcctg gaactcactc
     3121 tgtagaccag gctatcctca aactcagaaa tctgcctgcc tctgcctccc aagtgctggg
     3181 attaaaggca tgcgccacca cgcctggcac attttttttt taaattaaaa aaagaaagac
     3241 gttactaccc tgctcttgtt ttgtgacaca caatctggtc tgagaggacc ctgagcacat
     3301 cttccttcct tcaacactac cgtgctaagt tcttaaaatc tgggacttaa aaccaggtta
     3361 gtgacattac ccgtagttag gatgtttggt ttgttgggga ttggttctaa tgctctgtct
     3421 taattcggct cccagaatca cacgggaatc tgctctgcta aaggaagcct gtcactagtt
     3481 ggctgtgatt gggaaataaa gttgcccagg gctggctggg caggaaagag gcgggacttt
     3541 taggttgtga gggcaaggaa ccccggggag ttggaagcag agggatttca ctgcgcagtt
     3601 gggtctgggg cagcagagat gaaatgatga cttagcaagt cgactcaggg aggttagggg
     3661 ggtagaatgt atgctagtcg cacggagggt tagacacgtc cagccactga gctagtcaga
     3721 gcatatcaaa gttagatggt gtgtgtctct cattcacaaa tcccgggaac acttggccag
     3781 ccgggagtca ggggtctaag cactacaggg tttggaaacc agccaacact agaatctgca
     3841 cttgtgactg agcaggggta cggacaacag ctaacagtct acttgagctg cactgcggct
     3901 cagaagatca cttcccggag aaaattcacc ttggagtccg acatatctca cctttggaag
     3961 ctagaaacaa cttctaattt ccttcactgg aacaatgggt aaaaagccct cttgtaagct
     4021 agtgggggcc aatcagacca aatgtggcag aatgtagaac acctggttgg tgggacggga
     4081 agtcaggatt tattgggttg cggcttaatt aatgctcagc acagactgac tcctccttgg
     4141 taacgttcag cacactcgac agctctgaaa tccattccat ttctatacct taaaaagcag
     4201 tgtattttag aaacaattca aataaacatt tctctcgc
//
