LOCUS       NM_177327               6458 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus WW domain containing E3 ubiquitin protein ligase 1
            (Wwp1), transcript variant 1, mRNA.
ACCESSION   NM_177327
VERSION     NM_177327.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Imamura,M., Matsumoto,H., Mannen,H., Takeda,S. and Aoki,Y.
  TITLE     The R436Q missense mutation in WWP1 disrupts autoinhibition of its
            E3 ubiquitin ligase activity, leading to self-degradation and loss
            of function
  JOURNAL   In Vitro Cell Dev Biol Anim 60 (7), 771-780 (2024)
   PUBMED   38561589
  REMARK    GeneRIF: The R436Q missense mutation in WWP1 disrupts
            autoinhibition of its E3 ubiquitin ligase activity, leading to
            self-degradation and loss of function.
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6458)
  AUTHORS   Nozaki,Y., Kobayashi,M., Wakasawa,H., Hoshino,S., Suwa,F., Ose,Y.,
            Tagawa,R. and Higami,Y.
  TITLE     Systemic depletion of WWP1 improves insulin sensitivity and lowers
            triglyceride content in the liver of obese mice
  JOURNAL   FEBS Open Bio 13 (6), 1086-1094 (2023)
   PUBMED   37032433
  REMARK    GeneRIF: Systemic depletion of WWP1 improves insulin sensitivity
            and lowers triglyceride content in the liver of obese mice.
REFERENCE   4  (bases 1 to 6458)
  AUTHORS   Gorla,M., Chaudhari,K., Hale,M., Potter,C. and Bashaw,G.J.
  TITLE     A Nedd4 E3 Ubiquitin Ligase Pathway Inhibits Robo1 Repulsion and
            Promotes Commissural Axon Guidance across the Midline
  JOURNAL   J Neurosci 42 (40), 7547-7561 (2022)
   PUBMED   36002265
REFERENCE   5  (bases 1 to 6458)
  AUTHORS   Kishikawa,T., Higuchi,H., Wang,L., Panch,N., Maymi,V., Best,S.,
            Lee,S., Notoya,G., Toker,A., Matesic,L.E., Wulf,G.M., Wei,W.,
            Otsuka,M., Koike,K., Clohessy,J.G., Lee,Y.R. and Pandolfi,P.P.
  TITLE     WWP1 inactivation enhances efficacy of PI3K inhibitors while
            suppressing their toxicities in breast cancer models
  JOURNAL   J Clin Invest 131 (24) (2021)
   PUBMED   34907909
  REMARK    GeneRIF: WWP1 inactivation enhances efficacy of PI3K inhibitors
            while suppressing their toxicities in breast cancer models.
REFERENCE   6  (bases 1 to 6458)
  AUTHORS   Li,Y., Zhou,Z., Alimandi,M. and Chen,C.
  TITLE     WW domain containing E3 ubiquitin protein ligase 1 targets the
            full-length ErbB4 for ubiquitin-mediated degradation in breast
            cancer
  JOURNAL   Oncogene 28 (33), 2948-2958 (2009)
   PUBMED   19561640
REFERENCE   7  (bases 1 to 6458)
  AUTHORS   Feng,S.M., Muraoka-Cook,R.S., Hunter,D., Sandahl,M.A., Caskey,L.S.,
            Miyazawa,K., Atfi,A. and Earp,H.S. 3rd.
  TITLE     The E3 ubiquitin ligase WWP1 selectively targets HER4 and its
            proteolytically derived signaling isoforms for degradation
  JOURNAL   Mol Cell Biol 29 (3), 892-906 (2009)
   PUBMED   19047365
REFERENCE   8  (bases 1 to 6458)
  AUTHORS   Jones,D.C., Wein,M.N., Oukka,M., Hofstaetter,J.G., Glimcher,M.J.
            and Glimcher,L.H.
  TITLE     Regulation of adult bone mass by the zinc finger adapter protein
            Schnurri-3
  JOURNAL   Science 312 (5777), 1223-1227 (2006)
   PUBMED   16728642
REFERENCE   9  (bases 1 to 6458)
  AUTHORS   Conkright,M.D., Wani,M.A. and Lingrel,J.B.
  TITLE     Lung Kruppel-like factor contains an autoinhibitory domain that
            regulates its transcriptional activation by binding WWP1, an E3
            ubiquitin ligase
  JOURNAL   J Biol Chem 276 (31), 29299-29306 (2001)
   PUBMED   11375995
REFERENCE   10 (bases 1 to 6458)
  AUTHORS   Huang,K., Johnson,K.D., Petcherski,A.G., Vandergon,T., Mosser,E.A.,
            Copeland,N.G., Jenkins,N.A., Kimble,J. and Bresnick,E.H.
  TITLE     A HECT domain ubiquitin ligase closely related to the mammalian
            protein WWP1 is essential for Caenorhabditis elegans embryogenesis
  JOURNAL   Gene 252 (1-2), 137-145 (2000)
   PUBMED   10903445
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ101217.1, AK137588.1, AL683894.6, AK031762.1 and AK033138.1.
            
            On Jan 26, 2013 this sequence version replaced NM_177327.4.
            
            Transcript Variant: This variant (1) represents the longr
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK137588.1, SRR14777534.1935044.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                CJ101217.1         3-78
            77-395              AK137588.1         2-320
            396-396             AL683894.6         174082-174082       c
            397-1435            AK137588.1         322-1360
            1436-1436           AL683894.6         142731-142731       c
            1437-3117           AK137588.1         1362-3042
            3118-4530           AK031762.1         1051-2463
            4531-5813           AK033138.1         3346-4628
            5814-6458           AL683894.6         109126-109770       c
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 7.56 cM"
     gene            1..6458
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="WW domain containing E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:107568"
                     /db_xref="MGI:MGI:1861728"
     exon            1..166
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            167..262
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            263..353
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..3040
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1; WW
                     domain-containing protein 1; NEDD4-like E3
                     ubiquitin-protein ligase WWP1; HECT-type E3 ubiquitin
                     transferase WWP1"
                     /codon_start=1
                     /product="NEDD4-like E3 ubiquitin-protein ligase WWP1
                     isoform 1"
                     /protein_id="NP_796301.2"
                     /db_xref="CCDS:CCDS38697.1"
                     /db_xref="GeneID:107568"
                     /db_xref="MGI:MGI:1861728"
                     /translation="MATASPRSDTSDIHSGRLQLKVTVSSAKLKRKKNWFGTAIYTEV
                     IVDGEVKKTAKSSSSSNPKWDEQLIVNVTPQTTLEFRVWSHHTLKADALLGKATVDLK
                     QVLLTHNRKLEKVKEQLKLSLENKNGIVQTGELTVVLDGLVIEQEPVTNRSSSPPIEI
                     QQNGDALHENGDPATRTTPRLPVEGTIGIDNHVSTNTVVPNSCCSHVVNGENTPSSPS
                     QVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTT
                     TQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEP
                     LRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGR
                     VYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDP
                     YGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVR
                     YFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKIN
                     VSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMY
                     CLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRM
                     LSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGS
                     NILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLC
                     GMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGG
                     FAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEG
                     FGQE"
     exon            354..492
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            493..617
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            618..755
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            756..822
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1007
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1332
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1428
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1603
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1658
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1743
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1872
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1948
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2020
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2109
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2269
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2403
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2544
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2665
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2666..2770
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2867
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2940
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..6458
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3352..3357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="hexamer: ATTAAA"
     polyA_site      3381
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="major polyA site"
     regulatory      6246..6251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="hexamer: AATAAA"
     polyA_site      6268
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
     regulatory      6336..6341
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="hexamer: AATAAA"
     polyA_site      6361
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
     regulatory      6388..6393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="hexamer: ATTAAA"
     polyA_site      6410
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
     regulatory      6430..6435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
                     /note="hexamer: AATAAA"
     polyA_site      6458
                     /gene="Wwp1"
                     /gene_synonym="8030445B08Rik; AIP5; SDRP1; Tiul1"
ORIGIN      
        1 gggggagggc cgggtgtcgg cgcgcttggc gtgcgggatc cgagtggctg cgggcggcct
       61 gagctgcccg gccgacgcct gctgcgagat ggcgatcttg ggcgcggagg gctgaggagg
      121 cgccgccgcg gtggctgcgg gccccgggag ccgcagcttt gcgcggggtt gtctcctcgc
      181 cagactatga gctccttgac agaagaaatc gtgtcttatt catcttcgta tcctcagtgt
      241 ctggcacagt tcttgataca caagtcaagg tgaattttgg gacatggcca ctgcttcacc
      301 aagatctgac actagtgata tccacagtgg aaggttacag ttaaaggtaa cggtttctag
      361 tgccaaacta aaaagaaaaa agaactggtt tggaacagca atatatactg aagtcattgt
      421 agatggagaa gttaagaaaa cagcaaaatc cagtagttcc tctaatccaa aatgggacga
      481 gcagctgatt gtaaatgtga ccccacagac caccttggag ttccgagttt ggagtcacca
      541 cactttaaag gcagatgctc tattaggaaa agcaacagtg gacttgaaac aagtgctgtt
      601 aacacacaac agaaaattag aaaaagtgaa agaacaatta aaactttcct tggaaaacaa
      661 gaatggaata gtgcaaactg gggaactgac agttgtcctt gatggattag tgattgagca
      721 agagcctgta acaaaccgca gctcctcgcc acccatagaa attcagcaaa atggagatgc
      781 cttacatgag aatggagacc ctgcaacaag gacaactccc aggttgcctg ttgaaggtac
      841 tattggaatt gataatcatg tatcaaccaa tactgttgtt cccaactcct gctgttcaca
      901 tgtagttaat ggagaaaaca caccttcatc cccgtctcag gttgctgcca gacccaaaaa
      961 tgctccagct ccaaagccag tcacatctgc gcccaccagt gacacggtta atggagaatc
     1021 atcatctgtc ctagctgata atacttcaac catgggtact ttactaccat ctgaagatac
     1081 cacctcgact tccaattgca ctagcaccac cacccaagag cctcctgtac aagaaccccc
     1141 agcatcctca gaacacagtg aatgtattcc ctctgccagt gcggaagtgg gaccagaagc
     1201 tcggagcctc atagaccctg actctgattc tagaaataat tctgtgtttg acaaagtaag
     1261 gcagccagaa gggtgtgtag aacctctgcg gccacagtct ggaaacacca acacagaagc
     1321 tttgccatca gggtgggaac agagaaaaga tcctcatggt agaacctatt atgtggatca
     1381 taatactcga actactacat gggagagacc acagcctttg cctccaggtt gggaaagaag
     1441 agttgatgat cgtggaagag tttattatgt ggatcataac accagaacaa caacctggca
     1501 gcggcctact atggaatctg ttcgaaattt tgaacagtgg cagtctcagc ggaatcaatt
     1561 gcagggagct atgcaacaat tcaaccaacg atacctctat tcggcttcca tgttagctgc
     1621 agaaaatgac ccctatggac ccttaccacc gggctgggaa aaaagagtgg attcaacaga
     1681 cagagtttac tttgtgaatc acaatacaaa aacaacccag tgggaagatc caagaactca
     1741 aggcttacca aatgaagaac ccctgcccga aggctgggaa atcaggtata ctcgggaagg
     1801 tgttaggtac tttgttgatc ataacacgag aacaacaaca ttcaaagacc ctcgcaatgg
     1861 gaagtcatca gtaactaaag gtggtccaca gattgcttat gaacgcagct ttaggtggaa
     1921 gcttgctcac ttccgttact tgtgccagtc taatgcacta ccgagtcatg taaagatcaa
     1981 tgtgtcccga cagacattat ttgaagattc cttccaacag attatggcat taaaaccata
     2041 tgacttgagg aggcgattat atgtaatatt tagaggagaa gaaggtcttg attatggtgg
     2101 ccttgcaaga gaatggtttt tcttgctttc ccatgaagtt ttgaacccaa tgtattgctt
     2161 atttgagtat gcaggcaaga ataactattg tttacagata aatccagcat caactataaa
     2221 tcctgaccat ctttcatact tctgttttat tggtcgcttt attgcaatgg cactgttcca
     2281 tggaaagttt attgatactg gtttctcttt accattctac aagcgtatgc taagtaaaaa
     2341 acttactatt aaggatttgg aatctattga tactgaattt tacaactctc taatctggat
     2401 aagagataac aatattgaag aatgtggctt agaaatgtac ttttctgtag acatggagat
     2461 tctgggaaaa gttacttcac atgatttaaa gttgggaggt tccaatatcc tggtgacgga
     2521 agaaaacaaa gatgaatata ttggtttaat gacagaatgg cgtttttctc gaggagtaca
     2581 agaacagacc aaagctttcc ttgatgggtt taatgaagtt gttccccttc agtggctaca
     2641 gtactttgat gaaaaagaat tggaggttat gctgtgtggc atgcaagagg ttgacttggc
     2701 agactggcag agaaatactg tttatcgaca ttatacaaga aacagcaagc aaattatttg
     2761 gttttggcag tttgtgaaag agactgataa tgaagtacga atgcgactct tgcagtttgt
     2821 gactggaacc tgtcgcttgc ctctaggagg atttgctgag ctcatgggaa gtaatgggcc
     2881 tcaaaagttc tgtattgaaa aagttggcaa agacacctgg ttaccaagaa gccatacatg
     2941 ttttaatcgc ttggatctac caccatataa gagttatgaa caactaaagg aaaaacttct
     3001 ttttgcaata gaagagacag agggatttgg acaagaatga ctgacttgtc ttggagaagc
     3061 tcttgcattt aaatacccag ccaagaaaaa ttgcacagat agtgtgtata agctgctcat
     3121 tctgtacagt gaattttctg aacctttcaa agtataaatg ttttccgttc ttccacagaa
     3181 atatgcaaaa catttcatca ttttctactt tatttattgt tccctttaaa tgactgacca
     3241 ggaaaaaaaa atcatcctta aattttgacg caagcaagag aatttattaa aaagtatata
     3301 tctatataac catatatgac agtggctcta gttttataga gcgccaggtg tattaaacat
     3361 gacagccgtt cattcacaaa actctgaatt tgctttgcct tgtttctgtt ttccagggga
     3421 aaagtgcata tgtgccatgt tcttctccct aggtgacatt ggattgtgtt agctgcatgg
     3481 ctgttcagga aggaatttag ggcttaagcc aactcttact ttaagtgtat ttttttaaat
     3541 tgctgctggc ttttctgaag acaagtactt caactcatta agttgtacta aatgaatagg
     3601 ttttttttaa tcacatcatt gttatatcag tagacacaga agtcgtgtga tagatccagt
     3661 cgtgtctagg agcagctggg attgacttgc tgcagagcag aggtctattt acatcttact
     3721 ttcggatgtt gggtatttgt ggaaatttaa agaatcaggc atctctatac ttacattttt
     3781 aaacttgtaa tgttcttcaa attcaattgc taatgatttg atagtttaat gcttagggat
     3841 acataaaagg tactgaactt ggctgcttct gtgctcaagg tttacttgta tgaagtcaga
     3901 gatgttctaa tggattgaaa agctgcagaa gtatttaaac gctcacacta agtacttttt
     3961 attagtgttt gaatgtcccc accccttcct tacaaaatca gaattgtttc taaattcagt
     4021 aggtatatta aaagaattct ttgtgctccc tattttatac agagcaagtt ttctccaaat
     4081 gacactgatt tttcttttca ttataagggc attcttggtg tatgaaaaaa attaaaatct
     4141 aatgtgaatg atactgctac ttttagcaaa ctgggcttcc taattaatat aaaacattaa
     4201 tttccatttt tatcctttca atatgcactg cgaagttcat taacttaaaa ttttgtaact
     4261 atgaagttca cttagaatta taacttctat ccactagttt agttttgcta agattttatt
     4321 tttctacttt agattatgtt cttcagtttt attcagaatt cacaaataaa agcagtagtc
     4381 tacttagttg aataaactat agaaaatctg gctattctca agcttaaaag aattttttta
     4441 attctaacat atggcaagtt atatagtaaa tgatggccag tcttcaaaag tcctgcataa
     4501 attattctgt aagataataa acttgctatg acagaaagta tcggctttca ttcagtggag
     4561 gtatgtgaca gcatcccttt gcagatatgg tttacggttg ctttcaggta ggatgtcagg
     4621 gagcatccct tttgttaggt tacctcacgt gaggaacaga acccacaaga tcaagccagc
     4681 cttctgggga agactagtct gcagacctca tcaggttctt aacctttcat accagttata
     4741 gacttcaggc ctatagactt gtttatctac tcagtagtca aagtcttaaa gagaggtgaa
     4801 tcacccctgc tgtctccttc ccatccctct tctgaactgg accctccaca tctggctcac
     4861 tgaatccagt aagacaaagt tggaggtgag gaggaccgag agacaggtta ataatgatgc
     4921 tttattatag tgacagttcc caacatcacc agtaaattga gcttagggaa tggatcatcg
     4981 gcttaaaaca gttttacctg tcagctcagt cacctacctg tctttgggaa agaagctact
     5041 atttgtttta gaaaatgaaa atgttattac ccttttctcc agctgacttc aattatcctc
     5101 ctaggtctag gtgttctaaa ttaggtgttt tataaccact ccatcaacag accacctttg
     5161 ttgtgtttgg ggctcaaaaa gaaccctaac tagtaagctg cttctgaact gagaaagctc
     5221 ctgacatata ggagggagct gtaactgcgt ttcccagaat ctgaacaggc ttactattgc
     5281 atgtcctaaa ctgtcacatt aggagactac cgtttttgta gaaccatgtg atggccctgc
     5341 tatattacta ctacatgttc attttctcaa aggaggccta acattgcact gatggccatc
     5401 atggttcctg cactagagta ttcagagtaa actgatgcca gacaaatgta gtttactgca
     5461 ggcttactgg atcctgacat ttaattaaat ataacataat ctagtaaaca ctgaagtaac
     5521 aaatcagttg ttgaaacatg aaataaatgt tcttctgtaa catgtccaac ttttcttaat
     5581 gtgttgaaca gagaaatcaa gtcaactggg aatttgacac caatctaaat gtgctatttc
     5641 ctaaatgcta aaagttcgac tccaaaattc aacagaatgc aattaaaatg gtggtatttc
     5701 tttttattac taattctaca tctttttcat gaacacttaa ttctgttggt ccttgtgatt
     5761 aacgtagtat cttgttttca ttatgtttct taagaaacaa gacaagtctt accaatccac
     5821 tttgtgttgt agcatccatt gcctgttggt aggccacact gtcaagaagt gacagagaag
     5881 ttcagtggtg aacgtgaggg gaaagaggga aaccaaataa ccaaaaagac ccccagcttt
     5941 ctcctagtgt gctcagaaac ctgggccctt catccactac actaccagta aacaaaccct
     6001 aattcaggtt tcacacatcc tcaaagacag cagacacact taaggaaagg aaatcaccca
     6061 ttgcctgtgt ctatagtatg ttggcctcct ttttcctctc aaataatcag ccttctactt
     6121 tgatgtcttt ttaaaaaaaa tatggaccca catgcagtat ccatctgatc ctataatggc
     6181 ttaacaagat catcttcaat tctatccttt gactgcaaat gaggtaattt cactttttat
     6241 gactgaataa aactggtttg taaatatata cattttgttt gtccacttgg tcgatgtcta
     6301 tactgattcc atagcttgtt tgtaaacaat gccacaataa atatcaatgt aaatgccttt
     6361 atttttaata gccacaaatt taaaaatatt aaataccttc aacagatgga taaagtggta
     6421 tattcatgta ataaaatatt aacactaaaa ggaaaaaa
//
