LOCUS       NM_144783               3092 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus WT1 transcription factor (Wt1), mRNA.
ACCESSION   NM_144783 XM_001001558 XM_974407
VERSION     NM_144783.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Scavuzzo,M.A., Szlachcic,W.J., Hill,M.C., Ziojla,N.M., Teaw,J.,
            Carlson,J.C., Tiessen,J., Chmielowiec,J., Martin,J.F. and
            Borowiak,M.
  TITLE     Pancreatic organogenesis mapped through space and time
  JOURNAL   Exp Mol Med 57 (1), 204-220 (2025)
   PUBMED   39779976
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Franks,N.E. and Allen,B.L.
  TITLE     Hedgehog-dependent and hedgehog-independent roles for growth arrest
            specific 1 in mammalian kidney morphogenesis
  JOURNAL   Development 151 (24) (2024)
   PUBMED   39629522
REFERENCE   3  (bases 1 to 3092)
  AUTHORS   Oikonomakos,I., Tedesco,M., Motamedi,F.J., Peitzsch,M., Nef,S.,
            Bornstein,S.R., Schedl,A., Steenblock,C. and Neirijnck,Y.
  TITLE     In vitro differentiation of mouse pluripotent stem cells into
            corticosteroid-producing adrenocortical cells
  JOURNAL   Stem Cell Reports 19 (9), 1289-1303 (2024)
   PUBMED   39178848
REFERENCE   4  (bases 1 to 3092)
  AUTHORS   Teng,X., He,H., Yu,H., Zhang,X., Xing,J., Shen,J., Li,C., Wang,M.,
            Shao,L., Wang,Z., Yang,H., Zhang,Y. and Wu,Q.
  TITLE     LncRNAs in the Dlk1-Dio3 Domain Are Essential for Mid-Embryonic
            Heart Development
  JOURNAL   Int J Mol Sci 25 (15), 8184 (2024)
   PUBMED   39125754
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3092)
  AUTHORS   Song,L., Li,Q., Xia,L., Sahay,A.E., Qiu,Q., Li,Y., Li,H.,
            Sasaki,K., Susztak,K., Wu,H. and Wan,L.
  TITLE     Single-cell multiomics reveals ENL mutation perturbs kidney
            developmental trajectory by rewiring gene regulatory landscape
  JOURNAL   Nat Commun 15 (1), 5937 (2024)
   PUBMED   39009564
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3092)
  AUTHORS   Bruening,W. and Pelletier,J.
  TITLE     A non-AUG translational initiation event generates novel WT1
            isoforms
  JOURNAL   J Biol Chem 271 (15), 8646-8654 (1996)
   PUBMED   8621495
  REMARK    GeneRIF: Use of non-AUG (CUG) and AUG translation initiation sites
            result in multiple isoforms.
REFERENCE   7  (bases 1 to 3092)
  AUTHORS   Karavanova,I., Vainio,S. and Thesleff,I.
  TITLE     Transient and recurrent expression of the Egr-1 gene in epithelial
            and mesenchymal cells during tooth morphogenesis suggests
            involvement in tissue interactions and in determination of cell
            fate
  JOURNAL   Mech Dev 39 (1-2), 41-50 (1992)
   PUBMED   1489725
REFERENCE   8  (bases 1 to 3092)
  AUTHORS   Pelletier,J., Bruening,W., Kashtan,C.E., Mauer,S.M., Manivel,J.C.,
            Striegel,J.E., Houghton,D.C., Junien,C., Habib,R., Fouser,L. et al.
  TITLE     Germline mutations in the Wilms' tumor suppressor gene are
            associated with abnormal urogenital development in Denys-Drash
            syndrome
  JOURNAL   Cell 67 (2), 437-447 (1991)
   PUBMED   1655284
REFERENCE   9  (bases 1 to 3092)
  AUTHORS   Pelletier,J., Schalling,M., Buckler,A.J., Rogers,A., Haber,D.A. and
            Housman,D.
  TITLE     Expression of the Wilms' tumor gene WT1 in the murine urogenital
            system
  JOURNAL   Genes Dev 5 (8), 1345-1356 (1991)
   PUBMED   1651275
REFERENCE   10 (bases 1 to 3092)
  AUTHORS   Buckler,A.J., Pelletier,J., Haber,D.A., Glaser,T. and Housman,D.E.
  TITLE     Isolation, characterization, and expression of the murine Wilms'
            tumor gene (WT1) during kidney development
  JOURNAL   Mol Cell Biol 11 (3), 1707-1712 (1991)
   PUBMED   1671709
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL512584.13, DQ537939.1 and
            BG087452.2.
            
            On Dec 30, 2006 this sequence version replaced NM_144783.1.
            
            Summary: This gene encodes a transcription factor that contains
            four zinc-finger motifs at the C-terminus and a
            proline/glutamine-rich DNA-binding domain at the N-terminus. It
            plays an essential role in the normal development of the urogenital
            system, and the orthologous human gene is mutated in a small subset
            of patients with Wilm's tumors. Alternative splicing has been noted
            for this gene, however, the full-length nature of these variants is
            not known. The mRNA for this gene has been shown to initiate
            translation from non-AUG (CUG) and AUG translation start sites,
            resulting in different isoforms. [provided by RefSeq, Apr 2013].
            
            Sequence Note: A non-AUG (CUG) translation initiation codon is
            selected for this RefSeq based on experimental evidence provided in
            PMID:8621495, and for consistency with the human homolog.
            Alternative shorter isoforms, which are derived from downstream CUG
            and AUG initiation codons, are also described in PMID:8621495.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 8621495
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-914               AL512584.13        85808-86721
            915-1275            DQ537939.1         252-612
            1276-1691           BG087452.2         75-490
            1692-1700           AL512584.13        129289-129297
            1701-3092           AL512584.13        131502-132893
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 55.06 cM"
     gene            1..3092
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /note="WT1 transcription factor"
                     /db_xref="GeneID:22431"
                     /db_xref="MGI:MGI:98968"
     exon            1..914
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..1822
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /note="non-AUG (CUG) translation initiation codon; Wilms
                     tumor 1 homolog"
                     /codon_start=1
                     /product="Wilms tumor protein homolog"
                     /protein_id="NP_659032.3"
                     /db_xref="CCDS:CCDS16496.2"
                     /db_xref="GeneID:22431"
                     /db_xref="MGI:MGI:98968"
                     /translation="MDFLLSQEPASTCVPEPASQHTLRREPGCVQQPEQPGDRGPRSA
                     WAKSSAENPQDRRSGEPSASEPHLMGSDVRDLNALLPAVSSLGGGGGGCGLPVSGAAQ
                     WAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAF
                     TLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPTIRNQGYS
                     TVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDS
                     CTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSN
                     HGTGYESENHTAPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFM
                     CAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKP
                     FQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQR
                     NMTKLQLAL"
     exon            915..1037
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1140
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1218
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1269
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1366
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1517
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1607
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1700
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..3092
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3071..3076
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
     polyA_site      3088
                     /gene="Wt1"
                     /gene_synonym="D630046I19Rik; Wt-1"
ORIGIN      
        1 tgtgtgaatg gagcggccga gcatcctggc tcctcctcct tccctgctgc cggcccctct
       61 tatttgagct ttgggaagct gggggcagcc aggcagctgg ggtaaggagt tcaaggcagc
      121 gcccacaccc ggggctctcc gcaacccgac cgcctgcctg ctcccccttt ccttttcccg
      181 ccctccctcc cacccactca ttcacccacc cacccagaga gaggacggca gcccaggaac
      241 ccgggcccgc cgcctcctcg ccgcgatcct ggacttcctc ctgtcgcagg agccggcttc
      301 cacgtgtgtc ccggagccgg cgtctcagca cacgctccgc cgggagcccg ggtgcgtcca
      361 gcagccggag caacctgggg accgaggccc ccggagcgcc tgggccaagt ccagcgccga
      421 gaatccgcag gatcgcagga gcggagaacc gtccgcatcc gagccgcacc tcatgggttc
      481 cgacgtgcgg gacctgaacg cgctgctgcc cgctgtgtct tcgctgggcg gcggcggcgg
      541 cggctgcggg ctccctgtga gcggcgcagc gcagtgggcg cccgtgttgg acttcgcgcc
      601 tccgggcgcc tcggcttacg ggtcgctggg cggtcccgcg cctcctcccg ctccgccgcc
      661 gcctccgccg ccaccccact ccttcatcaa acaggagccc agctggggcg gcgccgagcc
      721 acacgaggag cagtgcctga gcgccttcac cttgcacttc tcgggccagt tcaccggtac
      781 agccggggcc tgtcgctacg gacccttcgg tcctcccccg cccagccagg cgtcctcggg
      841 ccaggccagg atgttcccca atgcgcccta cctgcccagc tgcctggaga gccagcctac
      901 catccgcaac caaggataca gcacggtcac tttcgacggg gcgcccagct atggccacac
      961 gccctcgcat cacgcggcgc agttccccaa ccattccttc aaacacgagg accccatggg
     1021 ccagcagggc tcgctgggcg agcagcagta ctccgtgcca cctccggtgt atggctgcca
     1081 cacccctact gacagttgca caggcagcca ggccctgctc ctgaggacgc cctacagcag
     1141 tgacaattta taccaaatga cctcccagct tgaatgcatg acctggaatc agatgaacct
     1201 aggagctacc ttaaagggaa tggctgctgg gagctccagc tcagtgaaat ggacagaagg
     1261 gcagagcaac cacggcacag ggtatgagag tgagaaccac acggccccca tcctctgtgg
     1321 tgcccagtac agaatacaca cccacggggt cttccgaggc attcaggatg tgcggcgtgt
     1381 atctggagtg gccccaactc ttgtccggtc agcatctgaa accagtgaga aacgtccttt
     1441 catgtgtgca tacccaggct gcaataagag atattttaag ctgtcccact tacagatgca
     1501 tagccggaag cacactggtg agaaaccata ccagtgtgac ttcaaggact gcgagagaag
     1561 gttttctcgc tcagaccagc tcaaaagaca ccaaaggaga cacacaggtg tgaaaccatt
     1621 ccagtgtaaa acttgtcagc gaaagttttc ccggtccgac catctgaaga cccacaccag
     1681 gactcataca ggtaaaacaa gtgaaaagcc cttcagctgt cggtggcaca gttgtcagaa
     1741 aaagtttgcg cgctcagacg aattggtccg ccatcacaac atgcatcaga gaaacatgac
     1801 caaactccag ctggcgcttt gaggggtccg acacggagac agtccagcat cccaggcagg
     1861 aaagtgtgca aactgcttcc aaatctgatt ttgaaattcc tcccactcac ctttcaaagg
     1921 acacgactgt ggatctacat ccgacttcca agacagcaca cctgattgac tgcatcctat
     1981 caggtttgcc ggaaggagtc ggtgctccgc ccacttttga ttaactcaca ggcctgaaaa
     2041 aagtggttca cggtgtctag aaagtccatt gctattgtct gaattttcta ctgttagaag
     2101 aaccattgtt gataatgccc cccgcccccc cccccgggtt tcctcttctc ctttgtgatc
     2161 atttccccag gattagagag actgttacat tttctttcat gggatattta taggccaggg
     2221 catgtgtatg tgcctgctaa tgtaaactct gtcatagttc ccatttacta actgccctag
     2281 aaagaaataa atcagagagc aaggcaccag gggcaagaat cgtgcagaat ttcagaggtc
     2341 tggctgcaaa cctggaaacc tggaaggcca gatgtaattc tacaggcgat tgttaaagct
     2401 cataggtttt gagtaactgc atagtaggtt ggtattaact agaactcctg tatagttagg
     2461 acagagagga gccttcctgc tcagctattc actctgaaca ctagcactgg gctcttaaga
     2521 aatgatgttt taagagcaga gatctttttt taatgtcttt gatttatttt ttagttgtaa
     2581 ttaggtacat cctcagagat gtactttcct cctcttgtgc aggatgtgga ggactcagtt
     2641 ccatcatctg gggcatcttt agagtgtata gaccacactg gttatgtggc ttcaagttgt
     2701 aaaaattaaa atgactttaa aagaaactag gggctggtcc aggatcttca ctggtaagac
     2761 tgttcttaag taacttaagt atctttgaat ctgcaagtat gtagggaaaa aaaaaagata
     2821 tattattgtg aggaaatcca ttgtttaaag gtgtgcgtgt gttgttgttg ttttttaaag
     2881 ggagggagtt tattatttac tgtagcttga aatactgtgt aaatatatat gtatatatat
     2941 gatgtgctct ttgtcaacta aaattaggag gtgtatggta ttagctgcat cactgtgtgg
     3001 atgtcaatct tacagtgtat tgatgataat actaaaaatg taacctgcat ctttttccac
     3061 ttggctgtca attaaagtct attcaaaagg aa
//
