LOCUS       NM_177292               4332 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus WSC domain containing 2 (Wscd2), mRNA.
ACCESSION   NM_177292
VERSION     NM_177292.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Ertunc,N., Phitak,T., Wu,D., Fujita,H., Hane,M., Sato,C. and
            Kitajima,K.
  TITLE     Sulfation of sialic acid is ubiquitous and essential for vertebrate
            development
  JOURNAL   Sci Rep 12 (1), 12496 (2022)
   PUBMED   35864127
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4332)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC096526.1.
            
            On Apr 25, 2009 this sequence version replaced NM_177292.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096526.1, SRR7974084.17662.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4332              BC096526.1         1-4332
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.55 cM"
     gene            1..4332
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /note="WSC domain containing 2"
                     /db_xref="GeneID:320916"
                     /db_xref="MGI:MGI:2445030"
     exon            1..494
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            495..1406
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     CDS             1007..2722
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /note="WSC domain-containing protein 2"
                     /codon_start=1
                     /product="sialate:O-sulfotransferase 2"
                     /protein_id="NP_796266.2"
                     /db_xref="CCDS:CCDS19550.2"
                     /db_xref="GeneID:320916"
                     /db_xref="MGI:MGI:2445030"
                     /translation="MAKLWFKFQRCFRYFRRKPVRFFTLLAIYLTAGSLVFLHSGFVG
                     QPAVPQSQASPAAGSPVEGAELPFLGDLHLGRGFRDTVEASSIARRYGPTFKGKDTSE
                     RAKLGDYGGAWSRALKGRVVREKEKEKEEEKAKYIGCYLDDTQSRALRGVSFFDYKKM
                     TVFRCQDNCAERGYLYAGLEFGAECYCGHKIQAANVSESDCDMECKGERGSVCGGINR
                     LSVYRLQLAQESARRYGSAVFRGCFRRPNNLSLALPVSAAMPNMSVDKCVDLCTEKEF
                     PLAALAGTACHCGFPTTRFPLHDREDEQLCAQKCSAEEFESCGTPSYFIVYQTQVQDN
                     RCMDRRFLPAKSKKLIALASFPGAGNTWARHLIELATGFYTGSYYFDGSLYNKGFKGE
                     RDHWRSGRTICIKTHESGQKEIEAFDAAILLIRNPYKALMAEFNRKYGGHIGFAAHAH
                     WKGKEWPEFVRNYAPWWATHTLDWLKFGKTVLVVHFEDLKQDLFTQLGRMVSLLGVAV
                     REDRLLCVESQKDGNFKRSGLRKLEYDPYTAEMRRTIAAYIRMVDTALRSRNLTGVPD
                     AYGPR"
     exon            1407..1521
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1706
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1828
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..2003
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2168
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2369
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..4302
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4274..4279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /note="hexamer: ATTAAA"
     polyA_site      4297
                     /gene="Wscd2"
                     /gene_synonym="4933413A10Rik; C530024P05Rik; Gm450"
                     /note="major polyA site"
ORIGIN      
        1 cacgctgaac acccatcaac aaaactgtcc tttgttggat ttctttgagc tgcctgccgg
       61 gcgggctcct ggccaagtct ccaagcagcg gcagccagcc cgcaccacgc tcaagccagg
      121 cgccggccct tcaccccgtc tcggtgcatt tcgccgcagc ccaggagcgg ggctcagcac
      181 actgcacagc ccggatccca ggcctgcggc gggcgcggcg ggaggccggg acgcaaggag
      241 ttaagcgcgg gcggtcgggc cgggctgcgg ggaggcggcg ggcgcgggag ctctggagct
      301 cgggctgcag actgctccag cagggccggg agtacgggcg cccgcgcagc cccagcctca
      361 tacccccggc ctcagcctcg ctcagtccca gccctggctc cgcgcaccac tctctgcgct
      421 ccaggctcca ggctcccggc ttcctgctcc tcgctgcgcc ctaccaggca cagagagctc
      481 ggcgcttccc ccaggtggta gcccggtggc ctgaagcttg accagatgga gtcctttcag
      541 cacatgtcca cagaaggtgg catacagctt ggccacctgt tagagataga gaagaggcca
      601 gcatcagagc tctctccgtg agctaatcag actcaacagc ccaaggggct aagaaggggt
      661 cagagtgact ttgcagccgc cagtccttgt cactagagtt ctttagccac actctgggtg
      721 aggaacaaaa tgtgaggaat ggagactgcg tcctgtaagt cccctttctt gaccccaagc
      781 agctcacctt gaccttggga ttccagcaac ctttgtcctt gtcacctctg gactcagcta
      841 ggtgcctggc ttgatttcct gctacagtgt ctctgtggag ggatcagaga gcgtcaagcc
      901 cttgcggttt cattcttgag aagtcccaag aagccacgcc ctccacgccc cttcccagcc
      961 atccacccag ccagccccaa gtgagcaagt ccggaatgat cctgctatgg ccaagctctg
     1021 gttcaagttc cagcggtgtt tccggtattt ccgcaggaag ccggtgcgct tctttaccct
     1081 gctggcaatc tacctgaccg ctgggagcct cgtcttcctg cactcgggct tcgtgggcca
     1141 gcctgcggtg ccccagagcc aggccagccc cgcagcgggg agcccggtag aaggagcgga
     1201 gctgcctttc ctgggtgatt tgcacctggg cagaggtttc cgggacacgg tggaggcctc
     1261 cagtattgca cgcaggtacg gacccacatt caagggcaag gacaccagcg agagagccaa
     1321 gctaggcgac tacggaggag cctggagccg agccctcaag ggaagggttg tccgggagaa
     1381 agagaaggaa aaggaggagg agaaagccaa gtacatcggc tgctacctgg acgacaccca
     1441 gagccgggcc ctgcgaggtg tgtccttttt cgactacaaa aagatgacag tcttccgttg
     1501 ccaggacaac tgtgctgaac ggggttatct gtatgctggg ctggagttcg gcgcagagtg
     1561 ctactgtggc cacaaaatcc aggcagccaa cgtgagcgaa tcagactgtg acatggaatg
     1621 caaaggagag cggggcagtg tgtgtggcgg catcaaccgt ctctcagtct accggctgca
     1681 gctggctcag gagtctgccc gaaggtatgg aagtgcagtg ttcaggggct gcttccgccg
     1741 acccaacaac ctctccctgg ccttgcccgt gtcagctgcc atgccaaaca tgtctgtgga
     1801 caagtgtgtg gacctctgca cagagaagga gttccccctg gcagccctcg ctggcactgc
     1861 ctgccactgt ggttttccca ccacacggtt ccctcttcat gaccgggagg atgagcaact
     1921 gtgtgcccag aagtgcagcg cggaggagtt cgagagctgt gggaccccca gctacttcat
     1981 cgtgtaccag acacaggtcc aagacaaccg ctgtatggac agacggttcc tcccagccaa
     2041 gtctaagaag ctcatcgctc tagccagctt cccgggcgct ggcaacacgt gggctcgcca
     2101 cctcatcgag ctggccaccg gcttctacac tggcagctac tactttgatg gctctctgta
     2161 caacaaaggg ttcaaaggtg agcgggacca ctggcgaagc gggcggacca tctgcatcaa
     2221 gacccatgaa agcggccaga aggagatcga ggccttcgac gcagccattc tgctcatccg
     2281 caacccctac aaggccctca tggctgagtt caaccgcaaa tatggaggcc acataggctt
     2341 tgccgcccac gcccactgga aaggcaaaga gtggcccgag ttcgtgagga actatgcccc
     2401 gtggtgggcc acgcacacgc tggactggct caagtttggc aagaccgtcc tggtggtgca
     2461 cttcgaggac ctgaagcagg acttgtttac gcagctgggc cgcatggtca gcctgctggg
     2521 cgtggcggtc agggaggatc ggctgctgtg cgtggagagc cagaaggacg gcaacttcaa
     2581 gcgctcgggg ctgcgcaagc tggagtatga cccttacaca gccgagatgc gcaggaccat
     2641 tgccgcctac atccgaatgg tggacacggc actacgtagc cgcaacctca ctggggtgcc
     2701 tgatgcctat ggcccaagat gatgggcagc cagggaggga cccagcatcc ggtgcccctt
     2761 ggcttcaaag agctggcttc cctctggcac aaagacagcc ccaggcttct gcctgccttc
     2821 agaaaggccc ctctgaggcc tcaggaggag gagatatcta gacacctttc cctggagagt
     2881 aggctgcctg gtttggagta tcccagaccc tgctattggt ggtgggagaa gggagaggct
     2941 atctggcaga ggttgggagg gtctggctgt ggcagctgat ggacattttg caagcaatga
     3001 ccctcttctg actgtgtggg cccctgcctc ttcccctgag caggcaccag ctttctgatc
     3061 tctctcaggc cacctctgtg tgacagacag ctggctggga cctgtctccg tggccttggg
     3121 cgtctcactt ctgaggacca tggagactgg tgctctggca ttacagtggg aaccgcccca
     3181 ggctaaagca cataagaggc ccaagccggg tgttgccccc cctccaggac agcaaccctg
     3241 tcttgtctgc tcgtgctgtc gtgtcttggg gatgagggtg acgttggggt tcttgatcct
     3301 tcttccaaag tcccttaggt accaaaaacc aacccaacag gttggccatg tgccacatac
     3361 ggaagcccag aggacccaac attgcagggt gggacctgaa ctggctatct tacactgcct
     3421 ggattgatcc cttaaccagg gagtccttcc actaccccag ggcagggaac aaaagtgctc
     3481 caactcagag agagccacac ccaaaactgt cccctaccct ctaagccatc agcctccctt
     3541 cccttgcttg caacccgaca gggccagccc caactgggga ctcctcagag tccccactaa
     3601 gaatggcttt ccattcccat tatgccgctg ctctttctcg aagacgctta gacaccaaag
     3661 gatttctgac tcaagtcact gatcacgttt taggaagcat gcatccagga tccccgtctc
     3721 ccccaccttg gatccctctg gcttcccctc cagcccttca ccgttcccat tacataaacg
     3781 caaaagccat agtcacagcc ccttgctctt ttgaccggag cagcccctcc cccacctcca
     3841 cccccgcagc ctggggggtg ggggggcggg gcaaatgaac actccttccc acctagccaa
     3901 ggaaccctgg agaagattcg tgaaaagaga gatatatttt tttaagagga atatgaccaa
     3961 gacattctgc cccttggaca ctggccacac aaaaaaggac tggcttccct tcccaacgcc
     4021 ctcatctgga gggtttggac caccccaggg ctcagccaag ggggctctga atgtattttg
     4081 tatggtgttt cccccgcccc agactaattt ctagttttct tttcactggg cagtagaggg
     4141 cctagcctcc cctcctgctt ggaggtctaa gctctctgag agactctggg tccaaaacat
     4201 gggccagttc tttgcaagtg gcctcctctg gtcacgaact ttctgttcga agattcctca
     4261 ggtcaacact atcattaaaa gagagttttg ttgctaactg gcaaaaaaaa aaaaaaaaaa
     4321 aaaaaaaaaa aa
//
