LOCUS       NM_011721               6383 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript
            variant 1, mRNA.
ACCESSION   NM_011721 XM_904113 XM_918670 XM_918681
VERSION     NM_011721.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Datta,A., Biswas,K., Sommers,J.A., Thompson,H., Awate,S.,
            Nicolae,C.M., Thakar,T., Moldovan,G.L., Shoemaker,R.H., Sharan,S.K.
            and Brosh,R.M. Jr.
  TITLE     WRN helicase safeguards deprotected replication forks in
            BRCA2-mutated cancer cells
  JOURNAL   Nat Commun 12 (1), 6561 (2021)
   PUBMED   34772932
  REMARK    GeneRIF: WRN helicase safeguards deprotected replication forks in
            BRCA2-mutated cancer cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Kang,S.M., Yoon,M.H., Lee,S.J., Ahn,J., Yi,S.A., Nam,K.H., Park,S.,
            Woo,T.G., Cho,J.H., Lee,J., Ha,N.C. and Park,B.J.
  TITLE     Human WRN is an intrinsic inhibitor of progerin, abnormal splicing
            product of lamin A
  JOURNAL   Sci Rep 11 (1), 9122 (2021)
   PUBMED   33907225
  REMARK    Erratum:[Sci Rep. 2021 Sep 1;11(1):17776. doi:
            10.1038/s41598-021-97463-5.. PMID: 34471172]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6383)
  AUTHORS   Aumailley,L., Dubois,M.J., Brennan,T.A., Garand,C., Paquet,E.R.,
            Pignolo,R.J., Marette,A. and Lebel,M.
  TITLE     Serum vitamin C levels modulate the lifespan and endoplasmic
            reticulum stress response pathways in mice synthesizing a
            nonfunctional mutant WRN protein
  JOURNAL   FASEB J 32 (7), 3623-3640 (2018)
   PUBMED   29452565
REFERENCE   4  (bases 1 to 6383)
  AUTHORS   Aumailley,L., Roux-Dalvai,F., Kelly,I., Droit,A. and Lebel,M.
  TITLE     Vitamin C alters the amount of specific endoplasmic reticulum
            associated proteins involved in lipid metabolism in the liver of
            mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant
            protein
  JOURNAL   PLoS One 13 (3), e0193170 (2018)
   PUBMED   29494634
  REMARK    GeneRIF: These results suggest that a great number of endoplasmic
            reticulum (ER) gene products are regulated at the
            post-transcriptional level in the liver of Wrn mutant mice
            exhibiting an ER stress response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6383)
  AUTHORS   Rekik,K., Frances,B., Valet,P., Dray,C. and Florian,C.
  TITLE     Cognitive deficit in hippocampal-dependent tasks in Werner syndrome
            mouse model
  JOURNAL   Behav Brain Res 323, 68-77 (2017)
   PUBMED   28119126
REFERENCE   6  (bases 1 to 6383)
  AUTHORS   Lebel,M. and Leder,P.
  TITLE     A deletion within the murine Werner syndrome helicase induces
            sensitivity to inhibitors of topoisomerase and loss of cellular
            proliferative capacity
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13097-13102 (1998)
   PUBMED   9789047
REFERENCE   7  (bases 1 to 6383)
  AUTHORS   Marciniak,R.A., Lombard,D.B., Johnson,F.B. and Guarente,L.
  TITLE     Nucleolar localization of the Werner syndrome protein in human
            cells
  JOURNAL   Proc Natl Acad Sci U S A 95 (12), 6887-6892 (1998)
   PUBMED   9618508
REFERENCE   8  (bases 1 to 6383)
  AUTHORS   Wu,J., He,J. and Mountz,J.D.
  TITLE     Effect of age and apoptosis on the mouse homologue of the huWRN
            gene
  JOURNAL   Mech Ageing Dev 103 (1), 27-44 (1998)
   PUBMED   9681877
REFERENCE   9  (bases 1 to 6383)
  AUTHORS   Imamura,O., Ichikawa,K., Yamabe,Y., Goto,M., Sugawara,M. and
            Furuichi,Y.
  TITLE     Cloning of a mouse homologue of the human Werner syndrome gene and
            assignment to 8A4 by fluorescence in situ hybridization
  JOURNAL   Genomics 41 (2), 298-300 (1997)
   PUBMED   9143515
REFERENCE   10 (bases 1 to 6383)
  AUTHORS   Yu,C.E., Oshima,J., Fu,Y.H., Wijsman,E.M., Hisama,F., Alisch,R.,
            Matthews,S., Nakura,J., Miki,T., Ouais,S., Martin,G.M., Mulligan,J.
            and Schellenberg,G.D.
  TITLE     Positional cloning of the Werner's syndrome gene
  JOURNAL   Science 272 (5259), 258-262 (1996)
   PUBMED   8602509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK171490.1, BC050921.1, AK052466.1 and AK162801.1.
            
            On Mar 25, 2008 this sequence version replaced NM_011721.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060700.1, AF091215.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2798              AK171490.1         2-2799
            2799-5074           BC050921.1         2744-5019
            5075-5880           AK052466.1         1968-2773
            5881-6383           AK162801.1         911-1413
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 20.3 cM"
     gene            1..6383
                     /gene="Wrn"
                     /note="Werner syndrome RecQ like helicase"
                     /db_xref="GeneID:22427"
                     /db_xref="MGI:MGI:109635"
     exon            1..186
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            187..291
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..4419
                     /gene="Wrn"
                     /EC_number="5.6.2.4"
                     /note="Werner syndrome ATP-dependent helicase homolog;
                     exonuclease WRN; Werner syndrome homolog; Werner syndrome
                     protein homolog"
                     /codon_start=1
                     /product="bifunctional 3'-5' exonuclease/ATP-dependent
                     helicase WRN"
                     /protein_id="NP_035851.3"
                     /db_xref="CCDS:CCDS22229.1"
                     /db_xref="GeneID:22427"
                     /db_xref="MGI:MGI:109635"
                     /translation="METTSLQRKFPEWMSMQSQRCATEEKACVQKSVLEDNLPFLEFP
                     GSIVYSYEASDCSFLSEDISMRLSDGDVVGFDMEWPPIYKPGKRSRVAVIQLCVSESK
                     CYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLESFVELTDVAN
                     EKLKCAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATDAYAGLIIY
                     QKLGNLGDTAQVFALNKAEENLPLEMKKQLNSISEEMRDLANRFPVTCRNLETLQRVP
                     VILKSISENLCSLRKVICGPTNTETRLKPGSSFNLLSSEDSAAAGEKEKQIGKHSTFA
                     KIKEEPWDPELDSLVKQEEVDVFRNQVKQEKGESENEIEDNLLREDMERTCVIPSISE
                     NELQDLEQQAKEEKYNDVSHQLSEHLSPNDDENDSSYIIESDEDLEMEMLKSLENLNS
                     DVVEPTHSTWLEMGTNGRLPPEEEDGHGNEAIKEEQEEEDHLLPEPNAKQINCLKTYF
                     GHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYTGKIGIVISPLISLM
                     EDQVLQLELSNVPACLLGSAQSKNILGDVKLGKYRVIYITPEFCSGNLDLLQQLDSSI
                     GITLIAVDEAHCISEWGHDFRSSFRMLGSLKTALPLVPVIALSATASSSIREDIISCL
                     NLKDPQITCTGFDRPNLYLEVGRKTGNILQDLKPFLVRKASSAWEFEGPTIIYCPSRK
                     MTEQVTAELGKLNLACRTYHAGMKISERKDVHHRFLRDEIQCVVATVAFGMGINKADI
                     RKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHLLWAPADFNTSRNLLIEIHDEKFRLY
                     KLKMMVKMEKYLHSSQCRRRIILSHFEDKCLQKASLDIMGTEKCCDNCRPRLNHCLTA
                     NNSEDASQDFGPQAFQLLSAVDILQEKFGIGIPILFLRGSNSQRLPDKYRGHRLFGAG
                     KEQAESWWKTLSHHLIAEGFLVEVPKENKYIKTCSLTKKGRKWLGEASSQSPPSLLLQ
                     ANEEMFPRKVLLPSSNPVSPETTQHSSNQNPAGLTTKQSNLERTHSYKVPEKVSSGTN
                     IPKKSAVMPSPGTSSSPLEPAISAQELDARTGLYARLVEARQKHANKMDVPPAILATN
                     KVLLDMAKMRPTTVENMKQIDGVSEGKAALLAPLLEVIKHFCQVTSVQTDLLSSAKPH
                     KEQEKSQEMEKKDCSLPQSVAVTYTLFQEKKMPLHSIAENRLLPLTAAGMHLAQAVKA
                     GYPLDMERAGLTPETWKIIMDVIRNPPINSDMYKVKLIRMLVPENLDTYLIHMAIEIL
                     QSGSDSRTQPPCDSSRKRRFPSSAESCESCKESKEAVTETKASSSESKRKLPEWFAKG
                     NVPSADTGSSSSMAKTKKKGLFS"
     exon            292..404
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            405..550
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            551..699
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            700..849
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            850..919
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1034
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1461
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1542
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1681
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1757
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1825
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1934
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2003
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2086
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2193
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2381
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2556
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2738
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2840
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2933
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3075
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3246
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3344
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..3417
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3491
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3492..3567
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3680
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3681..3795
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..3927
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            3928..4090
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            4091..4296
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     exon            4297..6383
                     /gene="Wrn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6361..6366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wrn"
                     /note="hexamer: AATAAA"
     polyA_site      6378
                     /gene="Wrn"
                     /note="major polyA site"
ORIGIN      
        1 gggcggggcc gcgggcggag tgcagggaag ggcagtcgcc agaccagaag tgcaccgagg
       61 cgcccgttgg tataaagtta gtaaatgtga ggcctctctc gatgcctggg tcctgggctt
      121 tggttctcag tcctccataa atcatcctgc tggaggagaa gacccttaga tctggctctt
      181 ctcaggggca ttttaaagac aaatgaaaat aaaatggaaa ccacttcact acagcggaaa
      241 tttccagaat ggatgtctat gcagagtcaa agatgtgcta cagaagaaaa ggcctgcgtt
      301 cagaagagtg ttcttgaaga taacctccca ttcttagaat tccctggatc cattgtttac
      361 agttatgaag ctagtgattg ctccttcctg tctgaagaca ttagcatgcg tctgtctgat
      421 ggcgatgtgg tgggatttga catggaatgg ccgcccatat acaagccagg gaaaagaagc
      481 agagtcgcag tgatccagtt gtgtgtgtct gagagcaaat gttacttgtt tcacatttct
      541 tccatgtcag ttttccccca gggattaaaa atgttactag aaaacaaatc aattaagaag
      601 gcaggggttg ggattgaagg ggaccagtgg aaacttctgc gtgattttga cgtcaagttg
      661 gagagttttg tggagctgac ggatgttgcc aatgaaaagt tgaagtgcgc agagacctgg
      721 agcctcaatg gtctggttaa acacgtctta gggaaacaac ttttgaaaga caagtccatc
      781 cgctgcagca attggagtaa tttccccctc actgaggacc agaaactgta tgcagccact
      841 gatgcttatg ctggtcttat catctatcaa aaattaggaa atttgggtga tactgcgcaa
      901 gtgtttgctc taaataaagc agaggaaaac ctacctctgg agatgaagaa acagttgaat
      961 tcaatctccg aagaaatgag ggacctagcc aatcgttttc ctgtcacttg cagaaatttg
     1021 gaaactctcc agagggttcc tgtaatattg aagagtattt cagaaaatct ctgttcattg
     1081 agaaaagtga tctgtggtcc tacaaacact gagactagac tgaagccggg cagtagtttt
     1141 aatttactgt catcagagga ttcagctgct gctggagaaa aagagaaaca gattggaaaa
     1201 catagtactt ttgctaaaat taaagaagaa ccatgggacc cagaacttga cagtttagtg
     1261 aagcaagagg aggttgatgt atttagaaat caagtgaagc aagaaaaagg tgaatctgaa
     1321 aatgaaatag aagacaatct gttgagagaa gatatggaaa gaacttgtgt gattcctagt
     1381 atttcagaaa atgaactcca agatttggaa cagcaagcta aagaagaaaa atataatgat
     1441 gtttctcacc aactttctga gcatttatct cccaatgatg atgagaatga ctcctcctat
     1501 ataattgaaa gtgatgaaga tttggaaatg gagatgctga agtctttaga aaacctaaat
     1561 agtgacgtgg tggaacccac tcactctaca tggttggaaa tgggaaccaa tgggcgtctt
     1621 cctcctgagg aggaagatgg acacggaaat gaagccatca aagaggagca ggaagaagag
     1681 gaccatttat tgccggaacc caacgcaaag caaattaatt gcctcaagac ctatttcgga
     1741 cacagcagtt ttaaaccggt tcagtggaaa gtcatccatt ctgtattaga agagagaaga
     1801 gataatgttg ttgtcatggc aactggatat gggaagagtc tgtgcttcca gtatccgcct
     1861 gtttatacag gcaagattgg cattgtcatt tcacctctca tttccttaat ggaagaccaa
     1921 gtcctccagc ttgagctgtc caatgttcca gcctgtttac ttggatctgc acagtcaaaa
     1981 aatattctag gagatgttaa attaggcaaa tatagggtca tctacataac tccagagttc
     2041 tgttctggta acttggatct actccagcaa cttgactcta gtattggcat cactctcatt
     2101 gctgtggatg aggctcactg catttcagag tggggccatg atttcagaag ttcattcagg
     2161 atgctgggct ctcttaaaac agcgctccca ttggttccag tcattgcact ctccgctact
     2221 gcaagctctt ccatccggga agacattata agctgcttaa acctgaaaga ccctcagatc
     2281 acctgcactg gatttgatcg gccaaatctg tacttagaag ttggacggaa aacagggaac
     2341 atccttcagg atctaaagcc gtttctcgtc cgaaaggcaa gttctgcctg ggaatttgaa
     2401 ggtccaacca tcatctattg tccttcgaga aaaatgacag aacaagttac tgctgaactt
     2461 gggaaactga acttagcctg cagaacatac cacgctggca tgaaaattag cgaaaggaag
     2521 gacgttcatc ataggttcct gagagatgaa attcagtgtg ttgtagctac tgtagctttt
     2581 ggaatgggca ttaataaagc tgacattcgc aaagttattc attatggtgc gcctaaggaa
     2641 atggaatcct attaccagga aattggtaga gctggccggg atggacttca gagttcctgt
     2701 cacttgctct gggctccagc agactttaac acatccagga atctccttat tgagattcac
     2761 gatgaaaagt tccggttata taaattaaag atgatggtaa agatggaaaa ataccttcac
     2821 tccagtcagt gtaggcgacg aatcatcttg tcccattttg aggacaaatg tctgcagaag
     2881 gcctccttgg acattatggg aactgaaaaa tgctgtgata attgcaggcc caggctgaat
     2941 cattgcctta ctgctaacaa ctcagaggac gcatcccaag actttgggcc acaagcattc
     3001 cagctactgt ctgctgtgga catcctgcag gagaaatttg gaattgggat tccgatctta
     3061 tttctccgag gatctaattc tcagcgtctt cctgataaat atcggggtca caggctcttt
     3121 ggtgctggaa aggagcaagc agaaagttgg tggaagaccc tttctcacca tctcatagct
     3181 gaaggattct tggtagaagt tcccaaggaa aacaaatata taaagacatg ttccctcaca
     3241 aaaaagggta gaaagtggct tggagaagcc agttcgcagt ctcctccgag ccttctcctt
     3301 caagctaatg aagagatgtt tccaaggaaa gttctgctac caagttctaa tcctgtatct
     3361 ccagaaacga cgcaacattc ctctaatcaa aacccagctg gattaactac caagcagtct
     3421 aatttggaga gaacgcattc ttacaaagtg cctgagaaag tttcttctgg gactaacatt
     3481 cctaaaaaaa gtgccgtgat gccgtcacca ggaacatctt ccagcccctt agaacctgcc
     3541 atctcagccc aagagctgga cgctcggact gggctatatg ccaggctggt ggaagcaagg
     3601 cagaaacacg ctaataagat ggatgtacct ccagctattt tagcaacaaa caaggttctg
     3661 ctggacatgg ctaaaatgag accgactact gttgaaaaca tgaaacagat cgacggtgtc
     3721 tctgaaggca aagctgctct gttggcccct ctgttggaag tcatcaaaca tttctgtcaa
     3781 gtaactagtg ttcagacaga cctcctttcc agtgccaaac ctcacaagga acaggagaaa
     3841 agtcaggaga tggaaaagaa agactgctca ctcccccagt ctgtggccgt cacatacact
     3901 ctattccagg aaaagaaaat gcccttacac agcatagctg agaacaggct cctgcctctc
     3961 acagcagccg gcatgcactt agcccaggcg gtgaaagccg gctaccccct ggatatggag
     4021 cgagctggcc tgaccccaga gacttggaag attattatgg atgtcatccg aaaccctccc
     4081 atcaactcag atatgtataa agttaaactc atcagaatgt tagttcctga aaacttagac
     4141 acgtacctca tccacatggc gattgagatt cttcagagtg gttccgacag cagaacccag
     4201 cctccttgtg attccagcag gaagaggcgt ttccccagct ctgcagagag ttgtgagagc
     4261 tgtaaggaga gcaaagaggc ggtcaccgag accaaggcat catcttcaga gtcaaagaga
     4321 aaattacccg agtggtttgc caaaggaaat gtgccctcag ctgataccgg cagctcatca
     4381 tcaatggcca agaccaaaaa gaaaggtctc tttagttaag atgacaacga tggaacagtt
     4441 tgtgtgtcct acatcttcat tcctataaag aatgaaaaga aatattttaa cctcaaaatt
     4501 atttaaagtc caaagtgaag ctcacctaaa cgtcgagcca tagagtcttt aattgcccgt
     4561 tggcagttga gctacagtat ctgaaccttc tgagacccgg agtgcagcat agactgtgaa
     4621 gtcggcttcc tttccgattg ccttccgaac ccgtgtcact gtcaggttgc agtctttctc
     4681 ttcttgcagc agtgtgtgtt ggaaatggag gctgtgtcgc tttgacatat agaacagatc
     4741 agtagttgca tagggacaga tatgaagata cagccggtct ttgctttctt atgcagatgc
     4801 ctgtatgaca gtatcagtgc accagcccag ccagggagac atcagcttcc atttaaaaag
     4861 ggaaagcgga caaggactcc agttacagaa acaactaaat tctatgcatt ttctgcacag
     4921 tctttattat ttctcaatca aaagtgttct ttgtactgaa tagtaaatat actaaatttt
     4981 cattttttaa attgttgtga gtgccttcaa tatttgaaga tgccaatttt taatgtctta
     5041 tgtttcacaa agaattaaaa aactgaaaat tcaactctgt tcttggtaaa ctattgttat
     5101 ataaatttat aagcagttta ccatatagct aaatatattc aaagccttgg attaactttt
     5161 tttttttttg taaatagaaa tggactgtta ttaatgtagg aaaaaatacc cctgaattca
     5221 gagaagttaa aaaaaagtca ctcagagttc cattaggaaa gaatcaaagc catttccttt
     5281 ttcaatcagc cagactccct cgtattcatt ttcttaattt gaatcttatt ataagacatt
     5341 caaactataa ttaaaacata gaaactccca cattctcatt tttctgcttt ctattaccag
     5401 aaggtagctt tccgtatatc acgcataatc tccagttgct tctatttctc tgtgccttaa
     5461 actgtttcta caatgcatga ggtggtattc tctgtggcag tggcagcact acagcgctcg
     5521 ggtccctgct gattgcggtg gcctgctgca ggaaaacgtc tgcttcattg ttttgactta
     5581 tgagttgaac tgggttttct ttccttcgtg gaattgaaaa acaaagcttg ggggcaaaaa
     5641 gtatgcttgg ttttttagac ttggttattt ggcagacatt ttctcaagat tcaatgaagt
     5701 tagcctgttg ctttaaggaa aatgacttac caataattat tgccagtaat aaaattgaga
     5761 tttctagcaa aaattacaat tctggtgaac aggtgtcctt cactgggcac ctgggtagct
     5821 ttccttgacc tgagggcttt tctaatgaga tgaataaaat aaacacatta tttttgatac
     5881 tatctaaagt ttcatctgta tctggaacat ctaagtgacc caagaaatca gtgtttaatg
     5941 gacaactaag acacgttata atatagcata tatagtaatg tagtcaagga agagcccatg
     6001 gccgtccctt cagatacaca ctgaaaataa tcacgaagca acagctacag ttttcatttc
     6061 aaagatttaa agtttcaaag attctctacc attatttgaa aagctatttt caaatagctt
     6121 ttcctaatat agcacacaca cacacacaca cacacacaca cacacacaca cacacagact
     6181 gaatacagta gaccagactc caggctcttc ttctcctgaa ctcatccaca caacgagaac
     6241 atttacagaa atgaaatggg gccatccttg tctttttttt ttaatgtaaa tgtgatttta
     6301 aaacgaacaa tatttgtgct aaattgtaat tggtttactg cagccatttt taaatgaatc
     6361 aataaatctt tgaaaaaagc ctc
//
