LOCUS       NM_001256224            5663 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus wingless-type MMTV integration site family, member 5A
            (Wnt5a), transcript variant 2, mRNA.
ACCESSION   NM_001256224
VERSION     NM_001256224.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Jasmine, Baraiya,D.H., Kavya,T.T., Mandal,A., Chakraborty,S.,
            Sathish,N., Francis,C.M.R. and Binoy Joseph,D.
  TITLE     Epithelial and mesenchymal compartments of the developing bladder
            and urethra display spatially distinct gene expression patterns
  JOURNAL   Dev Biol 520, 155-170 (2025)
   PUBMED   39798644
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Xie,N., Landin Malt,A., Adylkhan,A., Rodeman,N., Moraes Borges,R.,
            Hwang,D., Liu,A., Smith,C., Hogan,A. and Lu,X.
  TITLE     Wnt7b acts in concert with Wnt5a to regulate tissue elongation and
            planar cell polarity via noncanonical Wnt signaling
  JOURNAL   Proc Natl Acad Sci U S A 121 (35), e2405217121 (2024)
   PUBMED   39172791
  REMARK    GeneRIF: Wnt7b acts in concert with Wnt5a to regulate tissue
            elongation and planar cell polarity via noncanonical Wnt signaling.
REFERENCE   3  (bases 1 to 5663)
  AUTHORS   Paramore,S.V., Goodwin,K., Fowler,E.W., Devenport,D. and
            Nelson,C.M.
  TITLE     Mesenchymal Vangl1 and Vangl2 facilitate airway elongation and
            widening independently of the planar cell polarity complex
  JOURNAL   Development 151 (16) (2024)
   PUBMED   39225402
REFERENCE   4  (bases 1 to 5663)
  AUTHORS   Zhu,M., Gu,B., Thomas,E.C., Huang,Y., Kim,Y.K., Tao,H., Yung,T.M.,
            Chen,X., Zhang,K., Woolaver,E.K., Nevin,M.R., Huang,X.,
            Winklbauer,R., Rossant,J., Sun,Y. and Hopyan,S.
  TITLE     A fibronectin gradient remodels mixed-phase mesoderm
  JOURNAL   Sci Adv 10 (29), eadl6366 (2024)
   PUBMED   39028807
REFERENCE   5  (bases 1 to 5663)
  AUTHORS   Song,L., Li,Q., Xia,L., Sahay,A.E., Qiu,Q., Li,Y., Li,H.,
            Sasaki,K., Susztak,K., Wu,H. and Wan,L.
  TITLE     Single-cell multiomics reveals ENL mutation perturbs kidney
            developmental trajectory by rewiring gene regulatory landscape
  JOURNAL   Nat Commun 15 (1), 5937 (2024)
   PUBMED   39009564
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5663)
  AUTHORS   Burrus,L.W. and McMahon,A.P.
  TITLE     Biochemical analysis of murine Wnt proteins reveals both shared and
            distinct properties
  JOURNAL   Exp Cell Res 220 (2), 363-373 (1995)
   PUBMED   7556445
REFERENCE   7  (bases 1 to 5663)
  AUTHORS   McMahon,A.P., Joyner,A.L., Bradley,A. and McMahon,J.A.
  TITLE     The midbrain-hindbrain phenotype of Wnt-1-/Wnt-1- mice results from
            stepwise deletion of engrailed-expressing cells by 9.5 days
            postcoitum
  JOURNAL   Cell 69 (4), 581-595 (1992)
   PUBMED   1534034
REFERENCE   8  (bases 1 to 5663)
  AUTHORS   Gavin,B.J. and McMahon,A.P.
  TITLE     Differential regulation of the Wnt gene family during pregnancy and
            lactation suggests a role in postnatal development of the mammary
            gland
  JOURNAL   Mol Cell Biol 12 (5), 2418-2423 (1992)
   PUBMED   1373817
REFERENCE   9  (bases 1 to 5663)
  AUTHORS   Nusse,R., Brown,A., Papkoff,J., Scambler,P., Shackleford,G.,
            McMahon,A., Moon,R. and Varmus,H.
  TITLE     A new nomenclature for int-1 and related genes: the Wnt gene family
  JOURNAL   Cell 64 (2), 231 (1991)
   PUBMED   1846319
REFERENCE   10 (bases 1 to 5663)
  AUTHORS   Gavin,B.J., McMahon,J.A. and McMahon,A.P.
  TITLE     Expression of multiple novel Wnt-1/int-1-related genes during fetal
            and adult mouse development
  JOURNAL   Genes Dev 4 (12B), 2319-2332 (1990)
   PUBMED   2279700
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK036824.1 and CT025649.7.
            
            On Jan 19, 2018 this sequence version replaced NM_001256224.1.
            
            Transcript Variant: This variant (2) has multiple differences,
            compared to variant 1. These differences result in a distinct 5'
            UTR and cause translation initiation at a downstream start codon,
            compared to variant 1. The encoded protein (isoform 2) has a
            shorter N-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036824.1, SRR7345562.4275467.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3390              AK036824.1         2-3391
            3391-5663           CT025649.7         80967-83239         c
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 16.8 cM"
     gene            1..5663
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /note="wingless-type MMTV integration site family, member
                     5A"
                     /db_xref="GeneID:22418"
                     /db_xref="MGI:MGI:98958"
     exon            1..19
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /inference="alignment:Splign:2.1.0"
     exon            20..153
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..1156
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; protein Wnt-5a; wingless-related MMTV
                     integration site 5A"
                     /codon_start=1
                     /product="protein Wnt-5a isoform 2 precursor"
                     /protein_id="NP_001243153.1"
                     /db_xref="CCDS:CCDS56946.1"
                     /db_xref="GeneID:22418"
                     /db_xref="MGI:MGI:98958"
                     /translation="MSSKFFLMALATFFSFAQVVIEANSWWSLGMNNPVQMSEVYIIG
                     AQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTS
                     VFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWG
                     GCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVAC
                     KCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTT
                     QDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTER
                     CHCKFHWCCYVKCKKCTEIVDQFVCK"
     exon            154..404
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /inference="alignment:Splign:2.1.0"
     exon            405..697
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /inference="alignment:Splign:2.1.0"
     exon            698..5663
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3700..3705
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
                     /note="hexamer: AATAAA"
     polyA_site      3726
                     /gene="Wnt5a"
                     /gene_synonym="8030457G12Rik; Wnt-5a"
ORIGIN      
        1 acttgttgct ccggcccaga agcccattgg aatattaagc ccgggagtgg ctttggggac
       61 cgctggaggt gccatgtctt ccaagttctt cctaatggct ttggccacgt ttttctcctt
      121 cgcccaggtt gttatagaag ctaattcttg gtggtctcta ggtatgaata accctgttca
      181 gatgtcagaa gtatatatca taggtgcaca gcctctctgc agccaactgg caggactttc
      241 tcaaggacag aagaaactct gccacttgta tcaggaccac atgcagtaca ttggagaagg
      301 tgcgaagaca ggcatcaagg aatgccagta ccagttccgg catcggagat ggaactgcag
      361 cacagtggac aatacttctg tctttggcag ggtgatgcaa ataggcagcc gagagacggc
      421 cttcacgtac gcggtgagcg cagctggggt ggtgaacgcc atgagccgag catgccggga
      481 gggcgagctg tctacctgtg gctgcagccg cgctgcgcgc cccaaggacc tgcctcggga
      541 ctggttgtgg ggcggctgcg gagacaacat cgactatggc taccgcttcg ccaaggagtt
      601 cgtggacgct agagaaaggg aacgaatcca cgctaagggt tcctatgaga gcgcacgcat
      661 cctcatgaac ttacacaaca atgaagcagg ccgtaggaca gtatacaacc tggcagatgt
      721 agcctgtaag tgtcatggag tgtctggctc ctgtagcctc aagacgtgct ggctgcagct
      781 ggcggacttc cggaaggtgg gcgatgccct caaggagaag tatgatagcg cggcggccat
      841 gaggctcaac agccggggca agctggtgca ggtcaacagc cgcttcaact ccccgaccac
      901 gcaggacctg gtctacatcg accccagtcc ggactactgt gtgcgcaacg agagcactgg
      961 ctcgctgggc acgcagggac gcctgtgcaa caagacctca gaggggatgg acggctgcga
     1021 gctcatgtgc tgtgggcgtg gctatgacca gtttaagaca gtgcagaccg aacgctgtca
     1081 ttgcaagttt cactggtgct gctatgtcaa atgcaagaag tgcacggaga ttgtggatca
     1141 gttcgtgtgc aaatagtggt gtgcctgccc ttcacccagt cccactccca ggacccactt
     1201 atttatagaa agtacagtgc ttctggttct ttttatttct cccccaagaa ttgcagctgg
     1261 aaccatgtgt tttgttttgt tttattttgt tttttctttt ctgttaccat ctaagaactc
     1321 tgtggtttat tattaatatt ataattaata tttggcaata gtgggggaaa ctaagaaaaa
     1381 tatttatttt gaggatcttt gcaaagttag tacaaaattt ctttcttctg atgctacagg
     1441 ataaagggga aaaactatgt attcgaactt agctgtgcag ttgggggttc acatctagaa
     1501 ggtgtaggag ccattttctt ctcaaacaga gagtcctttg agatgggtgg tatccaggtg
     1561 aaggaggagg tacagaccca tgaataacag ttcctgtgac caaaatgaat tgcaggtgct
     1621 ctggtacaaa agatcttaaa tatagatata ttaaatatac atatatgcca aaaatacaga
     1681 atatgagaca ctccctaacc cagaggttac cagcctggtt ttgtgggttt tttgttttgt
     1741 tttgtttttt ctttttttgg gttttgtttg tttgtttgtt tgtttgtatt tttggtgtgt
     1801 gtgtgtgtgt atttctagaa tgatctttta gaaggtacaa gcaagaatct catatcttca
     1861 gaagcaggca tatcatgtat gttactgtgt cccacctaca gatactccat tcatgaatgg
     1921 gcctttttct aacagttcat gaatattggg gagccggtgg gctgggggag ggaggtcccc
     1981 agaaattaga aaacttgaag tttcctacat tgaggccata atcttgtgtt agcccagctg
     2041 attcttaata ccagactttt agatccataa aggaattttt gactaaaaaa aaaaaatctt
     2101 gttttgaaag ccatcttatt ttcttaaaaa tgaaaaatta cccatgaatc ccatttgcaa
     2161 cccctcaccc ccacaggcaa caagaaagtc ccatgtagtt gagcactgcg aacacctctg
     2221 tgaggagatg atggcagcca tcttcctgca tgatcccatg ccctttctgg actctctgct
     2281 ggccatgctt ccgaatggca gccctggtgg acactcactg ctggtagggc agaaaatgta
     2341 cacgaggagc catgttcaga accagccact taggggttgt tctctgaggc ttttctttgg
     2401 aggtacggta acttgatgtg ttttgatgat atctcttggc ccagggagtc cacagaggtg
     2461 ttgcagctgt ttggttgtta tcctcctgcg tttagacttt ccatttgtgc ttttcctatt
     2521 accctgcagg tgtaccctaa aactgttcct agtgtacttg aacagttgca tttataaggg
     2581 gggatgtggt ttaatggtgc ctgatatctc agtttttttg tatataacat atatataaat
     2641 atacatatat aaatatagat ataattatat ctcagtgcag tctgggattt agacctacag
     2701 ttttctctgg gcttgctctc tgcctggagt atcgtccttc attgcagtcc aattgggatt
     2761 tctttttttc caaaaatttt gagtcttaac attgacctgt gacaggatcc taccacgaat
     2821 accaggaagc aagctaagac tcggaggaag ctctcagggc tcatgtcctg aatgtatgtt
     2881 ggttagaaag tagcctttct gcttcctgcc catggccagt tctccaccct ctctttggtg
     2941 ttctttgtgg ggagggcact gtggtttgtc gcagccctgg acttcgagag gctcccagaa
     3001 cccaggatca ccagcctcct gtctgtttgc ttcactcctt tcccagggag gacttgggac
     3061 tgtcctgtct gacaggacgg atctgagttc ccgaagcaaa ccagctcacc acatagatag
     3121 ctagtttaaa caatgtttta aaataagggc acctctgttt caaaagtgac atctgctgtg
     3181 ttgttttcga ggcctgatac tcttacaagg tttgaaaaaa aatgtgtgta tccattcatg
     3241 ggcttggtag ccttctggtc acctcagtcc tgtggctctt aacttattgc ccaacaatat
     3301 tcatttcccc tcagctacaa tgaattgcaa gcaaaagatg ttgaaaaaaa gcactaattt
     3361 agtttaaaat gtcacttttt ggtttttatt ctacaaaaac catgaagttc tctctctctc
     3421 tctctctctc tcttatttgt taaatcagat tatgttcttt ttttgttttt gtttttagtg
     3481 attcatgttt atgagcagag tggagtttaa caatcctagc tttaaaaaaa acctatttaa
     3541 tgtaagatat tctacgcatc cttcagatat tttgtatatc ccctatggcc tttattctgt
     3601 acttttaatg tacatatttc tgtcttgtgt gatttgtata tttcactggt taaaaaaaag
     3661 aacattgaaa ggcttatgcc aatggaagat agaatataaa ataaaatgtt acttgtatat
     3721 tggtaagagg tttcagttgt ccttcagcta attcatgtag agaaatattt ttagttgaag
     3781 ccacaagaga cagcttaggg cagttatgtg ttcaaataac agaagaacag actttttttt
     3841 tttttttaaa ccaaacccaa actgttggga aacctcaata gagctctata tgtattggaa
     3901 caaaagtgga attctcttct cctatatatg ttccttcaaa aagagagaga gaatcaagca
     3961 gatggcttaa agctggtcac aggattgctc acattctttt ggcattatgc atgcgactta
     4021 attgtttgag agtgtgttgc tattgtaaca tcccagagat gaatcaaaaa ggctcaccct
     4081 ctcacccagg agcagctttt cagcttatat acacatgcat gtacatgtgt gtgatatgca
     4141 tgtgtgcatg catgtttgta tttttgtgct tgccactata actattgcac ctctctattc
     4201 ggtttgactg aagaggggtc ttgtgggaca tctctgtgtc ccagtcttta tgggaagaaa
     4261 gcaagggtct gcagagaaca ggaactaaag aatccctgtg tgatgtgcaa ttaatagaag
     4321 gcctcctgct ttctggaaat gtagaccaga atctggccag gactgtagac tgatacatta
     4381 tctggtcctt tgcctttttc ttttccctcc ctgcccctcc ccctcttgct ttatggataa
     4441 ccttgtaaca tattgaaacc tttaaaggaa accaagaatg cattattaca cacacacaca
     4501 cacacacaca cacacacaca cacacacaca cactacagta gaccaacata tagagtgttt
     4561 aaaatagctt ttctgggcaa attcaaacaa cttgtggctc taggacgcac atctgtttcc
     4621 gtttttcttc agttgtatat tgaccagtat tctttattgc taaaacatat actcggggta
     4681 gcaatgtcag catcttttcc cttcccatcc tggagagcat tcaagacctt cccagtacag
     4741 gaacatcaat gaagcattta tatacaggcg gtggcaagca gaaccacatc caaaatggtc
     4801 agtgtcgggc tctagggcaa ggctatcttg ttccagtcct gtttctttgt gctcctgacc
     4861 tttggggctg ccacttccca ggacgaccac tgcctgccca cactgtcccc ccctcccccc
     4921 ggggggattt tcccaatagc cagttcccat gtgtcttttt ctgcaacggt attcaagcca
     4981 atggaacctt cagatagggc ccaagagcag gatgacacaa cctgtggaca agagctatat
     5041 taacttgatc actagtatga gctaatatta acatgatcac ccatgaaagg cgcctgcaag
     5101 agctgtttag tctgaaatat aggtagagag cggggatggc aaggttgctt gtaacttctg
     5161 gtacatgttg aatgcacaca cgcatggagg caagctctaa atcactgcac tgttactgta
     5221 aagcatactt taaaaatatt tattgttttg aaaagcattt tctagtcttc cctctcttgg
     5281 tggagctgta aacaagatgg catgttgtga aggttcaaga tgattttttt ttaaatcgca
     5341 gaaacattta gacacctaag aactaaaact tataaaaggg atctttgaat ttgcctgtta
     5401 acatggatta atgtttacac ttacagctga tgattggacg gtgttttatg ttagggaaat
     5461 gccttgttaa cgaacttcat gaagcagatg taattaaagg ttgatgtgag ccaatctaga
     5521 aggttgaaca gtgttttcaa agaacggaga gacttacatt ttagaccaat ctttatacat
     5581 tttgctgagc tagaaaggag ataaagatta tttatttttg ttcatatctt gtacttttct
     5641 attaaaatca ttttatgaaa tcc
//
