LOCUS       NM_175638               4148 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus WNK lysine deficient protein kinase 4 (Wnk4), mRNA.
ACCESSION   NM_175638
VERSION     NM_175638.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ying,S., Guo,Q. and Zhang,C.
  TITLE     KLHL3-dependent WNK4 degradation affected by potassium through the
            neddylation and autophagy pathway
  JOURNAL   BMC Nephrol 24 (1), 217 (2023)
   PUBMED   37481568
  REMARK    GeneRIF: KLHL3-dependent WNK4 degradation affected by potassium
            through the neddylation and autophagy pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Xie,Z., Ma,X.H., Bai,Q.F., Tang,J., Sun,J.H., Jiang,F., Guo,W.,
            Wang,C.M., Yang,R., Wen,Y.C., Wang,F.Y., Chen,Y.X., Zhang,H.,
            He,D.Z., Kelley,M.W., Yang,S. and Zhang,W.J.
  TITLE     ZBTB20 is essential for cochlear maturation and hearing in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (24), e2220867120 (2023)
   PUBMED   37279265
REFERENCE   3  (bases 1 to 4148)
  AUTHORS   Izadifar,A., Courchet,J., Virga,D.M., Verreet,T., Hamilton,S.,
            Ayaz,D., Misbaer,A., Vandenbogaerde,S., Monteiro,L., Petrovic,M.,
            Sachse,S., Yan,B., Erfurth,M.L., Dascenco,D., Kise,Y., Yan,J.,
            Edwards-Faret,G., Lewis,T., Polleux,F. and Schmucker,D.
  TITLE     Axon morphogenesis and maintenance require an evolutionary
            conserved safeguard function of Wnk kinases antagonizing Sarm and
            Axed
  JOURNAL   Neuron 109 (18), 2864-2883 (2021)
   PUBMED   34384519
REFERENCE   4  (bases 1 to 4148)
  AUTHORS   Mayes-Hopfinger,L., Enache,A., Xie,J., Huang,C.L., Kochl,R.,
            Tybulewicz,V.L.J., Fernandes-Alnemri,T. and Alnemri,E.S.
  TITLE     Chloride sensing by WNK1 regulates NLRP3 inflammasome activation
            and pyroptosis
  JOURNAL   Nat Commun 12 (1), 4546 (2021)
   PUBMED   34315884
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4148)
  AUTHORS   Mukherjee,A., Yang,C.L., McCormick,J.A., Martz,K., Sharma,A. and
            Ellison,D.H.
  TITLE     Roles of WNK4 and SPAK in K+-mediated dephosphorylation of the NaCl
            cotransporter
  JOURNAL   Am J Physiol Renal Physiol 320 (5), F719-F733 (2021)
   PUBMED   33719576
  REMARK    GeneRIF: Roles of WNK4 and SPAK in K(+)-mediated dephosphorylation
            of the NaCl cotransporter.
REFERENCE   6  (bases 1 to 4148)
  AUTHORS   Kahle,K.T., Gimenez,I., Hassan,H., Wilson,F.H., Wong,R.D.,
            Forbush,B., Aronson,P.S. and Lifton,R.P.
  TITLE     WNK4 regulates apical and basolateral Cl- flux in extrarenal
            epithelia
  JOURNAL   Proc Natl Acad Sci U S A 101 (7), 2064-2069 (2004)
   PUBMED   14769928
  REMARK    GeneRIF: general role for WNK4 in the regulation of electrolyte
            flux in diverse epithelia
REFERENCE   7  (bases 1 to 4148)
  AUTHORS   Kahle,K.T., Wilson,F.H., Leng,Q., Lalioti,M.D., O'Connell,A.D.,
            Dong,K., Rapson,A.K., MacGregor,G.G., Giebisch,G., Hebert,S.C. and
            Lifton,R.P.
  TITLE     WNK4 regulates the balance between renal NaCl reabsorption and K+
            secretion
  JOURNAL   Nat Genet 35 (4), 372-376 (2003)
   PUBMED   14608358
REFERENCE   8  (bases 1 to 4148)
  AUTHORS   Yang,C.L., Angell,J., Mitchell,R. and Ellison,D.H.
  TITLE     WNK kinases regulate thiazide-sensitive Na-Cl cotransport
  JOURNAL   J Clin Invest 111 (7), 1039-1045 (2003)
   PUBMED   12671053
  REMARK    GeneRIF: WNK4 has a role in the sodium regulatory pathway of the
            distal
REFERENCE   9  (bases 1 to 4148)
  AUTHORS   Wilson,F.H., Kahle,K.T., Sabath,E., Lalioti,M.D., Rapson,A.K.,
            Hoover,R.S., Hebert,S.C., Gamba,G. and Lifton,R.P.
  TITLE     Molecular pathogenesis of inherited hypertension with hyperkalemia:
            the Na-Cl cotransporter is inhibited by wild-type but not mutant
            WNK4
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 680-684 (2003)
   PUBMED   12515852
  REMARK    GeneRIF: WNK4 negatively regulates surface expression of NCCT and
            implicate loss of this regulation in the molecular pathogenesis of
            an inherited form of hypertension.
REFERENCE   10 (bases 1 to 4148)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590969.12 and AY187027.1.
            
            On Dec 16, 2009 this sequence version replaced NM_175638.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096453.1, AY184228.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AL590969.12        128885-128959       c
            76-3793             AY187027.1         1-3718
            3794-4148           AL590969.12        112117-112471       c
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 64.51 cM"
     gene            1..4148
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /note="WNK lysine deficient protein kinase 4"
                     /db_xref="GeneID:69847"
                     /db_xref="MGI:MGI:1917097"
     exon            1..714
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..3774
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /EC_number="2.7.11.1"
                     /note="protein kinase, lysine deficient 4; protein kinase
                     with no lysine 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK4"
                     /protein_id="NP_783569.1"
                     /db_xref="CCDS:CCDS25459.1"
                     /db_xref="GeneID:69847"
                     /db_xref="MGI:MGI:1917097"
                     /translation="MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVR
                     RFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPTRSPPSSSKE
                     PPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVPDAAARERRREQEEKEDTETQ
                     AVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEE
                     VEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQR
                     WSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVI
                     GTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPN
                     SFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAEEDDGEKPGL
                     KLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVR
                     ERVAAIQRKREKLRKARELEVLPPDSGPPPATVSLAPGPPSAFPPEPEEPEADQHQSF
                     LFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGLPSSPAESHLCLPSGFALSI
                     PRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSD
                     RVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRV
                     ETLLKRDAGPPEAAEDALSPQEEPAALPALPGPPNAEPQRSISPEQRSWAAFSTSPSS
                     PGTPLSPGAPFSPGTPPVFPCPIFPITSPSCYPCPFSQVSSNPYPQAPSSLLPLSSSA
                     SQVPLPSSSLPISAPLPFSPSYPQDPLSPTSLPVCPSPPSLPSTTAAPLLSLASAFSL
                     AVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPLSLASCDQESLSAQTAETENEASRNP
                     AQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPSPPLTS
                     ESSDTEDSAAGGPETREALAESDRAAEGLGVAVDDEKDEGKEPLLGGSSPILSHPSPV
                     WMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRL
                     GKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGS
                     QEQRASKGVTFAGDIGRM"
     exon            715..887
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1108
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1266
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1355
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1572
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1837
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1959
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2018
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2133
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2250
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2388
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2431
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..3006
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3007..3067
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3473
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3474..3673
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3674..3771
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3772..4148
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4125..4130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /note="hexamer: AATAAA"
     polyA_site      4145
                     /gene="Wnk4"
                     /gene_synonym="2010002J11Rik; Pha2b; Prkwnk4"
                     /note="major polyA site"
ORIGIN      
        1 gggccccagc agctccgctg gagcgcggtg tacccagccg gtctgcctgt caggcagcaa
       61 cctccagcct cctggctttc cgttctgcgg cgcctggccc ttcccatgct agcacctcga
      121 aatacggaga ctggggtccc catgtcccaa accgaggctg acctggctct gcggccatct
      181 ccggctctca cctctacggg gccgacccgc ctggggccac ctcctcgccg agtgcgccgc
      241 ttctctggga aggctgagcc tcggccgcgc tcttcgagac ctagccgccg cagctcagtc
      301 gatctgggac tgctgagctc ctggtctcaa ccagcctcac tccttccgga acccccggat
      361 cctccagact ccgctggccc cacgaggagc ccaccttcaa gctctaaaga accccccgag
      421 ggcacatgga tgggggcagc tcccgtgaag gctgtggact ctgcatgtcc tgagcttacg
      481 ggatcttcag gaggtccagg gtcccgggag ccaccgaggg ttcctgatgc tgcagcccgg
      541 gaacggcggc gggagcagga agaaaaagag gacacagaaa cccaggctgt ggcaacgtct
      601 ccggacggcc gatacctcaa gtttgacatt gagattggaa gaggttcctt caagaccgtg
      661 tatcgagggc tagacaccga caccacggta gaggtggcct ggtgtgagct gcagacccgg
      721 aaactctctc gggccgagcg gcagcgtttc tccgaggaag ttgagatgct caaggggctg
      781 cagcacccca acatcgtccg cttctatgac tcctggaagt cggtgctgag gggccaggtt
      841 tgcatcgtgc tggtcacaga actcatgacc tcgggcacgc tcaagacgta cctgaggcgg
      901 ttccgcgaaa tgaagccccg agtccttcag cgctggagtc gccagattct acggggactt
      961 catttccttc attcccgagt gccccccatc ctgcaccgag atctcaagtg tgacaacgtc
     1021 ttcattactg gcccttcagg ctctgtcaaa atcggagacc tcggactggc cacgctcaag
     1081 cgcgcctcct ttgccaagag cgtcattggg accccggaat tcatggctcc tgagatgtac
     1141 gaggaaaagt acgacgaggc tgtggatgtg tacgcctttg gcatgtgcat gctggagatg
     1201 gctacgtctg agtatcccta ctccgagtgt cagaatgcag cacaaatcta ccgcaaggtc
     1261 acttcgggca caaagcccaa cagcttctac aaggtgaaga tgccagaggt gaaggagatc
     1321 attgaaggct gcatccgcac ggataagaac gagaggttca ccatccagga tctcctggcc
     1381 catgcgttct tccgcgagga acgaggtgtg cacgtggagc tggcagagga agatgatgga
     1441 gagaagccgg gcctcaagct ctggctgcgc atggaggatg cgcggcgcgg ggggcgtcca
     1501 cgagacaacc aggccatcga gttcctgttt cagctcggcc gggacgctgc ggaggaggtg
     1561 gctcaggaga tggtagcctt gggcttagtc tgtgaagctg actaccagcc agtggcccgt
     1621 gcagttcgag aacgggttgc tgctatccag cggaagcggg agaagcttcg aaaagctagg
     1681 gaattggagg ttctcccacc agactcagga cctcctccag caactgtgtc tctggctccg
     1741 ggtcccccca gtgccttccc cccagagcct gaggagccag aggctgacca gcatcaatcc
     1801 ttcctcttcc gccatgccag ctactcatca actacatctg attgcgagac tgatggctac
     1861 ctcagttcct ccggcttcct ggatgcctca gaccctgccc ttcagccccc tggggggtta
     1921 ccatccagcc ccgctgaatc ccatctctgc ttgccctcgg gttttgcctt gtctattcca
     1981 cggtctggcc ctggcagtga cttttctcct ggggatagct acgcctcaga tgcggcatca
     2041 ggccttagtg acatgggaga aggggggcaa atgaggaaaa atccagtgaa gactcttcga
     2101 cggagacctc gatcccggct ccgggtcact agtgtctcag accagagcga cagagttgtc
     2161 gagtgtcagc tgcagactca caacagcaag atggtgacgt tccgatttga tctggatggg
     2221 gacagcccag aagaaattgc agctgccatg gtatacaatg agttcatcct gccctcggag
     2281 cgagatggat tcctgagccg gatccgggag attatccagc gagtggagac cctgctgaag
     2341 agagatgctg gtcccccaga ggctgctgaa gatgccctga gcccccagga agagccagca
     2401 gccctgcctg ccctcccagg cccacccaat gcagagcccc agagaagcat ctccccagaa
     2461 cagaggagct gggcagcctt ctccacctct ccatcttctc ctggcacccc cttgtcccct
     2521 ggggccccct tttcccctgg gacccctcct gtcttcccat gccccatctt tcctatcact
     2581 tcaccctcat gctatccctg cccattctcc caggtctctt caaaccccta tccacaggcc
     2641 cccagttccc tgcttccctt atcctccagt gcttctcagg ttccacttcc atcttcctct
     2701 cttcccatca gcgctcccct cccattctct cctagttatc cccaagaccc tcttagcccc
     2761 acttctcttc ccgtctgccc ctctcctccc tctcttccct ctaccacagc agcccctctc
     2821 ctctctctgg ctagtgcctt ctctctggct gtgatgactg tggcccagtc cctgctgtcc
     2881 ccatcccctg gacttctttc tcagtctcct ccagcccctc caggtcctct gcctagcctg
     2941 cccctttccc ttgcttcttg tgaccaggag agcctttcgg cccaaacagc tgagacagag
     3001 aatgaggctt cccgtaatcc tgctcagcca ctactgggtg atgctagact ggcacctata
     3061 tctgaagagg gaaagcccca gcttgttggc cgtttccaag tgacttcatc taaggaacca
     3121 gcagagcctc ccctgcaacc agcatcccca actctctcca gatccctaaa gctgccaagc
     3181 cctccgctga cctcagagag ctcagacaca gaggacagtg ctgcaggagg cccagagact
     3241 agggaggctc tggcagagag tgaccgtgca gccgaaggcc tgggggttgc ggtcgatgat
     3301 gaaaaggatg aagggaagga acccctactt ggaggcagtt ccccaatctt gagccatccc
     3361 agcccagtgt ggatgaacta ctcctacagc agcctgtgtc tgagcagtga ggagtcggag
     3421 agcagcgggg aagacgagga attctgggct gagctgcaga accttcggca gaagcacttg
     3481 tcggaagtgg aggcactaca gacactacag aagaaggaaa tcgaggactt atacagccgg
     3541 cttgggaaac agcccccacc tggtattgta gctccagctg ctatgctgtc ctgccgccag
     3601 cgccgcctct cgaagggcag ctttcccacc tcccgccgca acagcctgca gcgctctgat
     3661 ctccctggtc ctggcatcat gcgaaggaac tctctgagtg gcagcagcac cggctcccag
     3721 gagcagcggg caagcaaggg ggtgacattc gccggggata ttggcaggat gtgaattcag
     3781 accagaagtc ctatgtctcc ccaacaccag ggccccacca tggcccatgg cctctcagaa
     3841 tctgatgttt tttgtgacca actcaactca gcaaagaaca ccccagcact gagtgggtag
     3901 aaggcccgtg ggcacggaga gtgcgttgtg ttgtatcctg aagagtcaaa accttgcggg
     3961 ttctctttgc tgtgtggaaa tctcatttct gtagcctacc ccacctgccc tccccaggca
     4021 agggctgacc actaagcaat ccgagtccag atgcttgcgg aaggccaact tccagctggg
     4081 agagtggagg ggctgtgctc ctgccaccaa agtcaacagc aaccaataaa aatactgagg
     4141 agaaaggc
//
