LOCUS       NM_001271679           10259 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus WNK lysine deficient protein kinase 3 (Wnk3),
            transcript variant 2, mRNA.
ACCESSION   NM_001271679
VERSION     NM_001271679.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Lim,W.M., Chin,E.W.M., Tang,B.L., Chen,T. and Goh,E.L.K.
  TITLE     WNK3 Maintains the GABAergic Inhibitory Tone, Synaptic Excitation
            and Neuronal Excitability via Regulation of KCC2 Cotransporter in
            Mature Neurons
  JOURNAL   Front Mol Neurosci 14, 762142 (2021)
   PUBMED   34858138
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   Izadifar,A., Courchet,J., Virga,D.M., Verreet,T., Hamilton,S.,
            Ayaz,D., Misbaer,A., Vandenbogaerde,S., Monteiro,L., Petrovic,M.,
            Sachse,S., Yan,B., Erfurth,M.L., Dascenco,D., Kise,Y., Yan,J.,
            Edwards-Faret,G., Lewis,T., Polleux,F. and Schmucker,D.
  TITLE     Axon morphogenesis and maintenance require an evolutionary
            conserved safeguard function of Wnk kinases antagonizing Sarm and
            Axed
  JOURNAL   Neuron 109 (18), 2864-2883 (2021)
   PUBMED   34384519
REFERENCE   3  (bases 1 to 10259)
  AUTHORS   Marcoux,A.A., Slimani,S., Tremblay,L.E., Frenette-Cotton,R.,
            Garneau,A.P. and Isenring,P.
  TITLE     Regulation of Na+-K+-Cl- cotransporter type 2 by the with no lysine
            kinase-dependent signaling pathway
  JOURNAL   Am J Physiol Cell Physiol 317 (1), C20-C30 (2019)
   PUBMED   30917032
  REMARK    GeneRIF: WNK kinase-dependent pathway can affect both the
            trafficking as well as intrinsic activity of NKCC2.
REFERENCE   4  (bases 1 to 10259)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   5  (bases 1 to 10259)
  AUTHORS   Zhang,J., Gao,G., Begum,G., Wang,J., Khanna,A.R., Shmukler,B.E.,
            Daubner,G.M., de Los Heros,P., Davies,P., Varghese,J.,
            Bhuiyan,M.I., Duan,J., Zhang,J., Duran,D., Alper,S.L., Sun,D.,
            Elledge,S.J., Alessi,D.R. and Kahle,K.T.
  TITLE     Functional kinomics establishes a critical node of volume-sensitive
            cation-Cl- cotransporter regulation in the mammalian brain
  JOURNAL   Sci Rep 6, 35986 (2016)
   PUBMED   27782176
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10259)
  AUTHORS   Cox,B.J., Vollmer,M., Tamplin,O., Lu,M., Biechele,S.,
            Gertsenstein,M., van Campenhout,C., Floss,T., Kuhn,R., Wurst,W.,
            Lickert,H. and Rossant,J.
  TITLE     Phenotypic annotation of the mouse X chromosome
  JOURNAL   Genome Res 20 (8), 1154-1164 (2010)
   PUBMED   20548051
REFERENCE   7  (bases 1 to 10259)
  AUTHORS   Leng,Q., Kahle,K.T., Rinehart,J., MacGregor,G.G., Wilson,F.H.,
            Canessa,C.M., Lifton,R.P. and Hebert,S.C.
  TITLE     WNK3, a kinase related to genes mutated in hereditary hypertension
            with hyperkalaemia, regulates the K+ channel ROMK1 (Kir1.1)
  JOURNAL   J Physiol 571 (Pt 2), 275-286 (2006)
   PUBMED   16357011
  REMARK    GeneRIF: WNK3 is likely a component of one of the mechanisms that
            determines the balance between renal NaCl reabsorption and K+
            secretion.
REFERENCE   8  (bases 1 to 10259)
  AUTHORS   Rinehart,J., Kahle,K.T., de Los Heros,P., Vazquez,N., Meade,P.,
            Wilson,F.H., Hebert,S.C., Gimenez,I., Gamba,G. and Lifton,R.P.
  TITLE     WNK3 kinase is a positive regulator of NKCC2 and NCC, renal
            cation-Cl- cotransporters required for normal blood pressure
            homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 102 (46), 16777-16782 (2005)
   PUBMED   16275913
  REMARK    GeneRIF: effects of WNK3 on these transporters and their
            coexpression in renal epithelia implicate WNK3 in NaCl, water, and
            blood pressure homeostasis, perhaps via signaling downstream of
            vasopressin
REFERENCE   9  (bases 1 to 10259)
  AUTHORS   Kahle,K.T., Rinehart,J., de Los Heros,P., Louvi,A., Meade,P.,
            Vazquez,N., Hebert,S.C., Gamba,G., Gimenez,I. and Lifton,R.P.
  TITLE     WNK3 modulates transport of Cl- in and out of cells: implications
            for control of cell volume and neuronal excitability
  JOURNAL   Proc Natl Acad Sci U S A 102 (46), 16783-16788 (2005)
   PUBMED   16275911
  REMARK    GeneRIF: suggest that WNK3 is part of the Cl(-)/volume-sensing
            mechanism necessary for the maintenance of cell volume during
            osmotic stress and the dynamic modulation of GABA neurotransmission
REFERENCE   10 (bases 1 to 10259)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JQ247190.1, BC043119.1, CF748862.1, CD350795.1, AK034405.1,
            CX566674.1 and BB676704.1.
            
            Transcript Variant: This variant (2) has an alternated splice site
            in the coding region, compared to variant 1. The resulting isoform
            (2) lacks an internal segment, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JQ247190.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5253              JQ247190.1         1-5253
            5254-8376           BC043119.1         1448-4570
            8377-8761           CF748862.1         227-611
            8762-9211           CD350795.1         268-717
            9212-9455           AK034405.1         415-658
            9456-10125          CX566674.1         78-747
            10126-10259         BB676704.1         301-434
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 68.46 cM"
     gene            1..10259
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /note="WNK lysine deficient protein kinase 3"
                     /db_xref="GeneID:279561"
                     /db_xref="MGI:MGI:2652875"
     exon            1..596
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..5195
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein kinase WNK3; protein kinase with
                     no lysine 3; protein kinase lysine-deficient 3; WNK lysine
                     deficient protein kinase 3, pseudogene"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK3 isoform 2"
                     /protein_id="NP_001258608.1"
                     /db_xref="CCDS:CCDS72450.1"
                     /db_xref="GeneID:279561"
                     /db_xref="MGI:MGI:2652875"
                     /translation="MATDSGEPASTEDSEKPDGVSFENRAARAVAPLTVEARIKEKYS
                     TFSASGENIERKRFFRKSVEMTEDDKVAESSRRDERKAATNISRVDKVPTNVLRGGQE
                     VKYEQCSKATSESSKDCFKEKTEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTV
                     YKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESTLKG
                     KKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLK
                     CDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFG
                     MCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSER
                     LSIKDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFS
                     FNLEADTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTLIKKIREKKPAGCLEERRDS
                     QCKYVRNVLPQQQTATLQPTPGPHTAAEYEETEVDQHVRQQFLQGKPQQQSSSVRGDT
                     SSEPTAGPVLHSDTSSHPTVAYSSNQTTSSQEQPKLTQSPVLPVVQGQSSVMPIYAAG
                     VGVVSQSQISPLTIQKVSQIKPVSQPIGAEQQATLQNPDFVRSLNQDVTSVKENTNNP
                     DTPSGNGKQDRNKQRRASCPRPEKGTKFQLTVLQVSVSGDNMVECQLETHNNKMVTFK
                     FDVDGDAPEDIADYMVEDNFVLENEKEKFVEELRAIVGQAQEILHVHSAVEKSIGVDS
                     VALESNSNQTGSSEQVLINSASTQTSNESAPQSSPVGRWRFCINQTIKNREAQSPPSL
                     QPSMAMVPGLHPFPSSRNTSNQAISQNTVFTIENNPGHRELFTSKLDHKDVVDGKIGE
                     HASIETEQSSISYQVEDDRQIMTPATDNSNYSAALVCPVPGECEALTSQAGMFMPTYP
                     NQQAAVLADVHIAYPGESVPIGGNAALTSVLVSSDQKPQSLSVQQPTIDAEFISKEGE
                     TTVNTETSSPKAVIATQTPGFEPAVILPATILESDGERPPKMEFADNRIKTLDEKLRN
                     LLYQEHSISSICPESQKDTQSIDSPFSSSAEDILSYSMPEVIAISHCGIQDSPAQSPN
                     FQQTGSKILSNVAASQPAHISVFKKDLNVITSVPSELCLHEMSPDASLPGDPEAYPAA
                     VSSDGTIHLQTGVETEDKRSAIASDPIPLTREFSSDTRALSRCKAMSGSFQRGRFQVI
                     TVPQQQPVKMMSFGKDHRPPFNKTTVQSSEQALTFAEAAVSQLIEVEPAMPTHKASVS
                     SRKLRTLYETFKEDKGDPEQGDIVSYSTACETSVSSVATEKNVTSTTEVSVQSGSEPL
                     DKEKNESTPGKQTCTNEFSATLAGNRKSVTKTRPEGDQYLPLREEQAYAQTQNSLFYS
                     PSSPMSSDNESEIEDEDLKVELQRLREKHIQEVVSLQTQQNKELQELYERLRATKDNK
                     AQSSEVPLSPASPRRPRSFKSKLRSRPQSMTHSDNLVVKDALGVESNTVSCQQSPASK
                     KGMFTDDLHKLVDDWTRETVGHFPSKPSLNQLKQSQQKSEAENWNKSCESTPSTMGYT
                     SNWISSLSQIRGAAPTSLPQGLPLPSFHGPLASYGMPHVCQYNAVGAAGYPVQWVGIS
                     GPAQQSVVLPTQSGGLFQPGMNLQSFPAPPVQNPASIPPGPK"
     exon            597..769
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            770..990
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1148
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1237
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1457
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1752
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1853
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1997
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2062
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2210
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2327
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..2483
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2484..2535
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2673
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..3620
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..3711
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3842
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            3843..4453
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            4454..4662
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            4663..4865
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     exon            4866..10259
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10202..10207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /note="hexamer: AATAAA"
     polyA_site      10219
                     /gene="Wnk3"
                     /gene_synonym="Prkwnk3; Wnk3-ps"
                     /note="major polyA site"
ORIGIN      
        1 cctgagatgt aagtagactg aagagtgaaa caagaacaaa tttagtgcca accttagtta
       61 caatggccac tgattctggg gagccagcta gcacagaaga ttctgagaag cctgatggag
      121 tttcctttga aaacagggct gcccgggctg ttgcccctct gactgtagaa gcgagaataa
      181 aggaaaaata cagtaccttt tctgcttccg gggaaaacat cgaaagaaag aggtttttcc
      241 gaaagagtgt tgagatgaca gaagatgaca aagttgccga atcttcccgc agagatgaaa
      301 gaaaagctgc aacaaatatt tcaagagtag ataaagttcc tacaaatgtg ctaagaggtg
      361 ggcaagaagt taaatatgag caatgttcaa aggcaacctc agaatcctca aaagattgct
      421 tcaaggagaa aactgaaaag gaaatggaag aagaagcaga aatgaaggct gttgcaactt
      481 cccctagtgg caggttcctg aaattcgata tagagctggg aagaggagca tttaaaacgg
      541 tatataaagg attggacact gagacatggg ttgaagttgc ttggtgtgag ctacaggacc
      601 gcaaattaac taaagctgag cagcaacggt tcaaagaaga agcagagatg ttgaaaggcc
      661 tccagcatcc caatatagtt cgattctatg attcatggga gtccactttg aaaggaaaga
      721 aatgtattgt gttagtgact gaactaatga catcaggaac cttaaagacg tacttaaaac
      781 gatttaaagt catgaaacca aaggtcttaa gaagctggtg caggcaaatc ttaaaaggat
      841 tgcagttctt acacacaagg acgcctccta ttattcaccg ggatctgaaa tgtgacaaca
      901 ttttcatcac aggacccact ggatctgtga agattggtga tctgggactt gcaaccttaa
      961 tgcgcacatc gtttgctaag agtgtcattg gcactcctga atttatggct ccggagatgt
     1021 atgaagaaca ctatgatgaa tctgtagatg tttatgcttt tggaatgtgt atgttggaaa
     1081 tggctacttc ggagtatcca tattctgagt gccagaatgc agcacaaata taccggaaag
     1141 taaccagtgg cataaaacca gccagcttca ataaagtcac agaccctgaa gtgaaagaaa
     1201 taattgaagg atgtattaga caaaacaaat ctgaaaggtt atctatcaag gacttactaa
     1261 accatgcatt ctttgctgag gatactggac tgagggtgga gttagcagag gaagatgatt
     1321 gctcaaattc atccttggct ctaagactct gggttgaaga tcctaagaaa ttaaaaggca
     1381 aacataaaga caatgaagct attgaattca gtttcaactt agaagcagat acacctgagg
     1441 aagtagcgta tgaaatggtc aagtctggat tcttccatga aagtgattcc aaagctgttg
     1501 ctaagtccat tagggaccga gtgacattga ttaaaaagat aagggaaaaa aagcctgctg
     1561 gatgcttgga ggaacgcagg gattcccagt gcaagtatgt gaggaatgta cttcctcaac
     1621 agcagactgc aactttacaa cctactcctg gtccacacac tgcagctgag tatgaagaaa
     1681 cagaagttga tcaacatgtt cgacagcagt ttctacaggg caaaccacag cagcagtcct
     1741 cctctgttag gggtgacact tcatctgagc caacagctgg accagttcta cattcagata
     1801 cttcaagtca tcccactgta gcctattctt caaaccagac aactagctct caggagcagc
     1861 caaagctgac tcagtcccca gttttgcctg tggttcaagg tcagtcctct gttatgccca
     1921 tatatgctgc tggagttgga gttgtttcac agtcccaaat ctctccctta actatccaga
     1981 aggtctcaca gataaagcct gtatcccaac caattggagc tgaacaacaa gcaactcttc
     2041 aaaatccaga ttttgttcga agcttgaatc aagatgtgac atctgtgaaa gaaaacacca
     2101 ataaccctga taccccaagt ggaaatggca aacaggatcg gaacaaacaa cgaagagcct
     2161 cctgtccccg accagagaag gggactaaat tccagcttac tgtccttcag gtctcagtct
     2221 ctggagacaa catggtggag tgtcagctgg aaacacataa caacaagatg gtgacattca
     2281 agtttgatgt tgatggtgac gcacctgagg atatcgctga ctatatggtt gaagataact
     2341 ttgtgctgga aaatgagaaa gaaaaatttg tcgaggaatt gagggctatc gtaggtcaag
     2401 cccaggagat ccttcatgtc cactcagctg tagaaaaatc tattggtgtt gactctgttg
     2461 ccttggaatc taacagtaac caaacaggat catctgaaca agtactgata aattctgcat
     2521 ccactcaaac tagcaatgaa tctgctcctc agtcatcccc agttggtcgc tggcggtttt
     2581 gtatcaacca aacaataaaa aaccgtgagg cacagtctcc tccttccctt cagccttcca
     2641 tggccatggt gcctgggtta catccatttc ctagttcaag aaacacaagt aatcaggcaa
     2701 tatcacagaa cacagtgttc actatagaga ataatccagg ccatcgtgaa cttttcacct
     2761 ccaaattaga ccacaaggat gtggttgatg gtaagattgg tgaacatgct agtatagaaa
     2821 ctgagcagtc aagtatttcc tatcaagtgg aagatgacag acagatcatg acaccagcta
     2881 ctgataattc caattattct gctgctttgg tgtgtccagt tccaggtgaa tgtgaggcac
     2941 tcaccagcca agcaggcatg ttcatgccta catatccaaa tcagcaagct gctgttctgg
     3001 ctgatgtgca tatagcctat cccggtgaat cagttccaat tggtggtaat gctgctctta
     3061 catcagtact tgtatcttct gatcaaaagc ctcaatcctt gtcagtacag cagccaacta
     3121 tagatgcaga gtttatttcc aaagaaggag aaaccacagt aaacaccgaa acaagttctc
     3181 ctaaggcagt cattgccact cagactcctg gctttgaacc agctgtgatt ctacctgcta
     3241 ctatcctgga atcagatggg gaacgacctc caaaaatgga gtttgcagac aaccgaatta
     3301 aaacactgga tgaaaaatta agaaacctgc tctatcagga gcacagcatt tctagcatct
     3361 gtcctgagag tcagaaggat actcaaagca tagactctcc attttcttcc tctgctgaag
     3421 atatactatc ctattcaatg ccagaagtca tagccatcag tcactgtggg attcaagata
     3481 gccctgcaca atccccaaat ttccaacaaa caggctctaa gattctgtcc aatgtggctg
     3541 caagtcagcc tgctcatata tcagtgttca aaaaagacct gaatgtgata acttctgtac
     3601 ccagcgagtt atgtttacat gagatgtccc cagatgcttc acttccaggc gatccagagg
     3661 cctatcctgc tgctgtgtca agcgatggaa ccattcatct gcagacagga gtggaaacag
     3721 aagataaaag atcagcaatt gcttctgatc ccattcctct gacacgggag ttctcatctg
     3781 atactagggc tttgagtaga tgtaaagcga tgagtggatc atttcagcgg ggtcggttcc
     3841 aggtgattac tgttcctcag cagcagccag taaaaatgat gtcttttggg aaagatcaca
     3901 gaccgccatt caacaaaaca acagtccagt ctagtgagca agcactaacc tttgctgaag
     3961 ctgcagtgtc gcagttgatc gaagtggagc ctgccatgcc cactcataaa gcttcagttt
     4021 cttcacgcaa gttgcgaact ctttatgaaa cctttaaaga agataaagga gatcctgaac
     4081 aaggtgacat cgtatcttac agcacagctt gtgagaccag tgtctcttca gtcgctacag
     4141 aaaagaatgt aacttcaacc acagaagtta gtgtgcagtc tgggtctgaa ccgttagata
     4201 aagaaaaaaa tgagtcaact cctgggaaac agacatgtac aaatgaattt tcagccactc
     4261 ttgctggtaa tagaaagtcc gtgacaaaaa ctcgtccaga gggtgatcag tatttaccac
     4321 tccgtgaaga gcaagcctat gctcaaacac agaattcact cttctattct ccatcttccc
     4381 caatgagcag tgacaatgaa tcagaaattg aggacgagga cttgaaagtg gagcttcaaa
     4441 gattacgaga aaaacacatt caagaggtgg tcagtcttca aacacaacag aataaagagc
     4501 tacaagagct ctatgaacgc cttcgagcaa ctaaagacaa caaagcgcaa tcttcagagg
     4561 ttcctttgtc acctgcatcg ccacgcaggc caagatcttt caaaagcaaa cttcgaagcc
     4621 gcccccagtc catgacacat tcagacaatc tcgttgtaaa agatgcactg ggtgtggaga
     4681 gtaataccgt gtcatgccag caatctccag ccagtaagaa ggggatgttc acagatgatt
     4741 tacacaagct ggtggatgac tggacaagag aaacagtagg gcattttcct agtaagccaa
     4801 gtttaaacca actcaaacag agccaacaaa aatccgaagc agaaaactgg aacaagtcat
     4861 gtgaaagtac tccatctact atgggataca cgtcaaactg gatttcttct ctctcccaaa
     4921 ttcgtggagc tgccccaact tccttgccac aaggactccc actcccttca tttcatgggc
     4981 cattagcatc atatggaatg cctcacgttt gtcagtataa tgctgtgggt gccgcaggtt
     5041 atccagtaca gtgggtagga atttctggac cagcacaaca gtctgtagtg cttcctaccc
     5101 aatctggggg actcttccag cccgggatga atttgcagtc atttccagct ccaccagtgc
     5161 agaatccagc ttcaatccct cctggtccta aatgaatcca agttgatgct gaaggaaggg
     5221 gagagaactt tcagaacacc tgtgatgcta cactttcttc ttgagttctg ccgtattttc
     5281 tctcattcgt gtctacttta aactgtgttt tttttgttgt tccagtgtga tatttgatac
     5341 agctcatctt tctgtagtta atgtgcaatt tgcacaaaga atactacaca cagaaatgtt
     5401 ttttcacttc aactcctatc ctctttgata cttgattttt aaaaaaatac tgttccgaat
     5461 gctttactag cccagcttaa gtgttaccat ttccaagaca ccttccatgt gttgcggaga
     5521 tgcgctcacc ccgcctaacc tcttttttat gctgctacca gcagttgtgt gagacttctc
     5581 tttaacatgt gtaaaaaagt atgtggattg gttctcccca aatgtggatg acgtaagacc
     5641 ctccaatagc cagtcttgtg ggattcgtca tgtctatctt agtttttact tttcacagaa
     5701 ggcagtaact ggctgcagga tatgtgttga tgtgtaaatg tagtagactt ttgtcaagca
     5761 gcacaaaagc tgagcgtttt gaatgtgact ttctccccgt catcacagca ggaggagtga
     5821 gggtgttcag tgaggcggag ggaaaggcaa aagcagaact gtaacagctc cttgaatcta
     5881 cagctactag agctgctcct gggttgcagt aacaacccag agcggccgtc ttttctcatg
     5941 tgcacacacc actttgcttt tgcgcatcca agaggcactg gtttggcttg cattcttttg
     6001 ctaggcactg cagctgatgt aaatctcatt tgggggtaat ccttgcaatc ctaaccaagg
     6061 agattcaggt taacacttct gttaatccat tcttctaaag tgaaatgtac ttgacacact
     6121 agggtttgta gcttttgaga cttttgcata aaacactggc tttacagggt tctaagagat
     6181 gtaggataca cactgtcctg cagtgaaata gtacacccta ggatgatatt atttaaattg
     6241 cctttaacct tttttgatga caatgtttgg gttgttatca tttcttacta gtgtctatag
     6301 agttagaaca ttttagatct tagatgcgtt tgtcagcctc ttgagaattt gagagacttc
     6361 actgtcaagt tgcactttac tgggttttat ccagtttttg gaggaaggga ggcagtgtca
     6421 aaatgtacac ctttggtttt tagggaggtt aactttagta atcttaagcg ctagtcacag
     6481 ggttacccaa ttttcagtct gtgaaaccca aatagaattt ttacttggtt tatatcaacc
     6541 cctctgtata catgttcaaa tgacttacca ttcattgaaa ctttgaaagt cctttcaaat
     6601 acatgttttc ctatttagga attctcttgg taaccattcc agactgtggt ctttgtttct
     6661 gttaaccaat tttcctccat attcctctaa accctacccc catgggcttg ccgggaagtg
     6721 agaaaactta atgtatgtgc ttttttcata caatttcata cgttgtgaca ttcagtaccc
     6781 ttcttaaaag ttgtcttaca ttgcttacct aaagaccaca cctgtcatca catttttatc
     6841 actgtttctg ggggcactcc gtggattgct attggtaaga gccacagttc cttttcctaa
     6901 gctagcatgt tttggatgag accagatcat atgcacggat tcctcaaatt ccttccatgg
     6961 tattgctttg catttgaagt gattttcaag ctattcatgt agtgcagttg cttgcattgt
     7021 agtgttttcc acaatgctcc taaaccccag agttctttaa aactgtttca ttatcagttg
     7081 gttttgtgta attatattgg gctaaataat ctataaggtg aaacactaca ggttttgaga
     7141 ctggatctcc tctagttgca atccttttaa acagtgcccc atggtgaaaa caaattccac
     7201 agatgacatg tacaattcca tttactaata gctgctctac atagtatgtc ctgtgttttg
     7261 ttacacatct cagttttaca tataattcat tgttcttgtt tgcctgcaga tgtttgcagt
     7321 gccttgtaat atttagaatc ttttcaggtt ttgctggaaa gaaaagtcat cttctaaagt
     7381 ttgaagatgg aactagtgta ctgctgtctc catgctgact gcagtatctg ggttaacata
     7441 tatgttacat atacattatg catatgtaat gtgtagaagt taaagcttac aaaaaaaggg
     7501 agtgtgcaga aatgaaaaca gtgctattca ctttgctgtg gggttttctt tactttccct
     7561 cattgctttt tgatttgatt cagattatag tcactttgat tgtaaatact gattagtttt
     7621 ctaggtggga attttgtatt gtttcataat tttaataagt cttataatca tgctgtttcc
     7681 atccttgcct gaggtacttt tcttccccca taaataatga attccagata acgctcagaa
     7741 gtaccctttt tctttaacaa aaagcctagt ctatataata agccttctgt tcttttctag
     7801 aaagtgcgca aacctatcct ttttgctttt tcttgtaagc aacacaaagg ttgcacttag
     7861 ccaccatctt gtaaagtttc cttgcgaaat agtcggtatg tccacccatt ctgtttcccc
     7921 ctccaagcgc acatctgatg agcagtgttg tgtactcatg tgtggtgaca tgagcaagtc
     7981 accatgaagc actgctcttg ggtctgtgga gttgggtgta acagaagact ggaaccagta
     8041 ctgctgtatt cagtgcagaa ccctccatat acctggagca gtcttgaatc taagcaattt
     8101 gctgtagttt cacaaatatc cctgcaggtt ttcatctctt ccttgcttat accaagcaga
     8161 agttctaata gtttgacggg aaggaaacct aactgtgaac gtttaaaagg cacttccgct
     8221 gtgatttttg ggttgagtgt agagtgtttt ctaaggtctg atgcaatgtt atcatctgaa
     8281 aaccccagat ttactggcag tccagttact tttgtttatc tacctcgttt ttcagtactg
     8341 ttttaggtgc acaagtaaat gttgcctgtg aaacttgctt ttttgccttg attctttgtg
     8401 ttagtgtaga ttctcagtct ggcttgcctt ttctgtccta gcactcacca tgcatgtggg
     8461 atgagaggaa ccatcactct gccaccaaat ctctgcttaa gtgatgacag ccatgcacct
     8521 gtgtgtaata aagttagggg ctgggggatg gaaagcaaag agagaaatta aattggtggc
     8581 aatacaggac ttggtggtgg gagctagttt tcaaatgtac tgaggtgaga cagccccagt
     8641 gcccccaact tcatatgtac tggtgatatt ttcttactca gtgcctaaag ggctatgggt
     8701 tgtaattgct ttaactacca cagtgaaata cttgacctct tagggcagtg acccatcttc
     8761 aaaatgttat cagactgcct ggtatttaca gtgacagttt gttatcttaa aatggcagaa
     8821 tgcatttcat ttggatctta gttggtagta agcttttaga taattgctta tctaatctct
     8881 gtagtttaat ggacttgact ctctttgctg tattttctaa gcattgaaaa ttctcttccc
     8941 ccctttaaaa aactcagcaa atgtttgtaa ctggatattc cttgtttcac atgtttactg
     9001 tgtaacacta catttttaat attagtaaat actatatttc agtcgtcatt tataagtaca
     9061 ttcctgaggt atatttgcca tcaatttgtg attcagttac ctcatgtttt ctttgattaa
     9121 gaaaaacctg agccttctga ggtggtatgc aagccatttt ctagaataga ttggtttttt
     9181 ttttaatctg tagttaatca gggacttatt ttccctccct ttatttagaa gatataatag
     9241 aaaggtatgt atatatagaa aagacttttg atttggtaaa taaaattcta gggttaaaat
     9301 ttaaaactct aacccaaatt atgaaatgaa aacactcgaa catagtacca gttcttttta
     9361 cataaaatta gtgtgcaatt ggtatagaga catactttca gaccaccctg gaaagagtct
     9421 cttttatatg tgtatgaagg atgaattcaa acattgatca gggaccttga caactatcat
     9481 gtgaatgggc ccacagtagt aatcaggtta aagtgatttt ccactgagat ggttgaaaga
     9541 atgtacagat caacagctcc gattctggga ggttctccgt gaagtaaaac aacccagact
     9601 gttaaaaagc taaaggtcag gtgagaaggc aaggggaaga ctacactctg aagggacgat
     9661 gcatgaaaat gcacacttgg cacccttcta gcttttttac attgtgtttc ttcccactat
     9721 aatttaagat ttaaggttga gatttgtagt ctagacccct cagcaggtag agtggataca
     9781 gtgactggtc agaaagtgca gttgcaggtc tgtcatgctc aatcacttaa cgctataatg
     9841 tgtttcctgc gaatatcaag catgtagacc aagtgcagca tgggagccac tcaggaagtt
     9901 tatgaaatta aactttcatg cataatttac catgaaatat gtagatttca gccaattctc
     9961 aatacttaac atttagttat atatgtgaac tctcctttct taattcggga atgtagcatt
    10021 atatagaatg ctgttaaact taattttaac cctttttaac tttttttttt ttttggtaag
    10081 ccaagtgatc tacctttcag cagttgtctg gttttgattc gtcgaaactg tgatttttat
    10141 tttaattact atccaactgt tgttaaatat tttgtttcct ggaattttgt tttgaaataa
    10201 aaataaaggc tgtgttaaat gagtatgtca ccttattaaa attctgtcat atttactgc
//
