LOCUS       NM_001199084           11346 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus WNK lysine deficient protein kinase 1 (Wnk1),
            transcript variant 5, mRNA.
ACCESSION   NM_001199084
VERSION     NM_001199084.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Biggs O'May,J., Vanes,L., de Boer,L.L., Lewis,D.A., Hartweger,H.,
            Kunzelmann,S., Hayward,D., Llorian,M., Kochl,R. and
            Tybulewicz,V.L.J.
  TITLE     WNK1-dependent water influx is required for CD4+ T cell activation
            and T cell-dependent antibody responses
  JOURNAL   Nat Commun 16 (1), 1857 (2025)
   PUBMED   39984435
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  AUTHORS   Li,L., Xie,D., Yu,S., Ma,M., Fan,K., Chen,J., Xiu,M., Xie,K., Li,Y.
            and Gao,Y.
  TITLE     WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to Enhance
            the Oxidative Stress Response in Hepatocellular Carcinoma
  JOURNAL   Cancer Res 84 (17), 2776-2791 (2024)
   PUBMED   38885324
  REMARK    GeneRIF: WNK1 Interaction with KEAP1 Promotes NRF2 Stabilization to
            Enhance the Oxidative Stress Response in Hepatocellular Carcinoma.
REFERENCE   3  (bases 1 to 11346)
  AUTHORS   Ferdaus,M.Z., Terker,A.S., Koumangoye,R.B., Al-Qusairi,L.,
            Welling,P.A. and Delpire,E.
  TITLE     Deletion of KS-WNK1 promotes NCC activation by increasing WNK1/4
            abundance
  JOURNAL   Am J Physiol Renal Physiol 327 (3), F373-F385 (2024)
   PUBMED   38961847
  REMARK    GeneRIF: Deletion of KS-WNK1 promotes NCC activation by increasing
            WNK1/4 abundance.
REFERENCE   4  (bases 1 to 11346)
  AUTHORS   Cesari,E., Farini,D., Medici,V., Ehrmann,I., Guerra,M., Testa,E.,
            Naro,C., Geloso,M.C., Pagliarini,V., La Barbera,L., D'Amelio,M.,
            Orsini,T., Vecchioli,S.F., Tamagnone,L., Fort,P., Viscomi,M.T.,
            Elliott,D.J. and Sette,C.
  TITLE     Differential expression of paralog RNA binding proteins establishes
            a dynamic splicing program required for normal cerebral cortex
            development
  JOURNAL   Nucleic Acids Res 52 (8), 4167-4184 (2024)
   PUBMED   38324473
REFERENCE   5  (bases 1 to 11346)
  AUTHORS   Jiang,G., Cai,Y., Cheng,D., Wang,H., Deng,G. and Xiang,D.
  TITLE     CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in
            osteoporosis by deubiquitinating WNK1
  JOURNAL   J Orthop Surg Res 19 (1), 212 (2024)
   PUBMED   38561786
  REMARK    GeneRIF: CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in
            osteoporosis by deubiquitinating WNK1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11346)
  AUTHORS   O'Reilly,M., Marshall,E., Speirs,H.J. and Brown,R.W.
  TITLE     WNK1, a gene within a novel blood pressure control pathway,
            tissue-specifically generates radically different isoforms with and
            without a kinase domain
  JOURNAL   J Am Soc Nephrol 14 (10), 2447-2456 (2003)
   PUBMED   14514722
  REMARK    GeneRIF: Several WNK1 transcript classes are demonstrated, showing
            tissue-, developmental-, and nephron-segment-specific expression.
REFERENCE   7  (bases 1 to 11346)
  AUTHORS   Yang,C.L., Angell,J., Mitchell,R. and Ellison,D.H.
  TITLE     WNK kinases regulate thiazide-sensitive Na-Cl cotransport
  JOURNAL   J Clin Invest 111 (7), 1039-1045 (2003)
   PUBMED   12671053
REFERENCE   8  (bases 1 to 11346)
  AUTHORS   Choate,K.A., Kahle,K.T., Wilson,F.H., Nelson-Williams,C. and
            Lifton,R.P.
  TITLE     WNK1, a kinase mutated in inherited hypertension with hyperkalemia,
            localizes to diverse Cl- -transporting epithelia
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 663-668 (2003)
   PUBMED   12522152
  REMARK    GeneRIF: findings suggest that WNK1 plays a general role in the
            regulation of epithelial Cl(-) flux [WNK1]
REFERENCE   9  (bases 1 to 11346)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 11346)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133738.1, BC171955.1, AC113092.25, CN693598.1, BC145282.1 and
            BC054836.1.
            
            Transcript Variant: This variant (5) represents the longest
            transcript and encodes the longest isoform (5). This variant
            represents the exon combination of the dorsal root ganglia and
            sciatic nerve variant described in Figure 2F of PubMed ID 18521183.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164139,
                              SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-996               AK133738.1         3-998
            997-3165            BC171955.1         1-2169
            3166-3867           AC113092.25        15640-16341         c
            3868-4583           CN693598.1         1-716
            4584-7155           BC145282.1         2352-4923
            7156-8994           BC171955.1         4639-6477
            8995-11346          BC054836.1         597-2948
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 56.86 cM"
     gene            1..11346
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /note="WNK lysine deficient protein kinase 1"
                     /db_xref="GeneID:232341"
                     /db_xref="MGI:MGI:2442092"
     exon            1..1782
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             1024..8931
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /EC_number="2.7.11.1"
                     /note="isoform 5 is encoded by transcript variant 5;
                     protein kinase with no lysine 1; serine/threonine-protein
                     kinase Wnk1; protein kinase lysine-deficient 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK1 isoform 5"
                     /protein_id="NP_001186013.1"
                     /db_xref="CCDS:CCDS57444.1"
                     /db_xref="GeneID:232341"
                     /db_xref="MGI:MGI:2442092"
                     /translation="MSDGAAEKQSGTPGFLTPPAPVPKNGSSSDSSVGEKLGATVADS
                     GVGRTEEYRRRRHTMDKDSRGAAATTTPTEHRFFRRSVICDSNATALELPGLPLSIPQ
                     PSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAASPGEQAVVGSATTTVPSSTSK
                     DRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEAQEDRSQQQDDIEELETKAVGMSNDG
                     RFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQ
                     HPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKG
                     LQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAP
                     EMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIP
                     EVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIED
                     IKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIK
                     RKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQQLSAASTGIPTAPATSASV
                     STQVEPEEPEADQHQQLQYQQPSISVLSDGTIDSGQGSSVFTESRVSSQQTVSYGSQH
                     EQAHSTGTAPGHTVSSIQAQSQPHGVYPPSSMPQSMAHPCGGTPTYPESQIFFPTIHE
                     RPVSFSPPPTCPPKVAISQRRKSTSFLEAQTRHFQPLLRTVGQNHLPPGSSPTNWTPE
                     AIVMLGATANRVNRELCEMQVQPVFEPTQIYSDYRPGLVLAEEAHYFIPQETVYLAGV
                     HYQAQVAGQYEGISYNSPVLSSPMKQISEQKPVPGGPASSSVFEFPSGQAFLVGHLQN
                     LRLDSGPSPASPLSSISAPNSTDATHLKFHPVFVPHSAPAVLTNSNENRSNCVFEFHA
                     QTPSSSGEGGGILPQRVYRNRQVAVDSNQEELSPQSVGLHCHLQPVTEEQRNNHAPEL
                     TISVVEPMGQIWPIGSPEYSSDSSQITSSDLSDFQSPPPTGGTAAPFGSDVSLPFIRL
                     PQTVLQESPLFFCFPQGTTSQQVLSASYSSGGSTLHPQAQGQNQGQPSSSLAGVLSSQ
                     PIQHPQQQGIQPTVPSQQAVQYSLPQAASSSEGTTAQPVSQPQVSAGTQGFPSRLPPQ
                     YPGDSNIAPSSNVASVCIHSTVLAPPSMPTEALATQGYFPTVVQPYVESTPLVPMGSV
                     GGQVQVSQPAVSLTQQPPTTSSQQAVLESTQGVSQAAPPEQTPITQSQPTQPVPLVTS
                     ADSAHSDVASGMSDGNENAPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNV
                     SNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVAQVRE
                     IIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFPGSQKLEGEFKQPIAVSSMPQQIG
                     VPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSDISDPVVASTSQAPGMNLSHSAS
                     SLSLQQAFSELKHGQMTEGPNTAPPNFNHMAGPTFSPFLASIAGVQTVAASTPSVSVP
                     ITSSPLNDISTSVMQSETALPTEKGIVGVTTTSTGVVASGGLTTMSVSESPTSSSAVS
                     SSTVPAVVTVSTPSQPVQASTSGSIASSTGSFPPGTFSTTTATTMGSVVAPDAKPPTV
                     LLQQVASNTAGVAIVTSVSTTTPFPGMASQPSLPLSSSTSAPTLAETMVVSAHSLDKA
                     SHSSTAGLGLSFCAPSSSSSSGTAVSTSVSQPGMVHPLVISSAVVSTPGLPQPVVPTS
                     TPLLPQVPNIPPLVQPVVNVPAVQQTLIHSQPQPALLPNQPHTHCPEMDADTQSKAPG
                     IDDIKTLEEKLRSLFSEHSSSGTQHASVSLETPLVVETTVTPGITTTAVAPSKLMTST
                     TSTCLPPTSLPLGAAGMPVMPVGTPGQVSTPGTHASAPVGTATGVKPGTTPPKPTKTV
                     VPPVGTELSAGTVPCEQLPPFPGPSLIQSQQPLEDLDAQLRRTLSPETITVAPAVGPL
                     STMSSTTVTEAGTRLQKDGTEGHVTATSSGAGVVKMGRFQVSVTMDDAQKERKNRSED
                     TKSVHFESSTSESSVLSSSSPESTLVKPEPNGISISGISLDVPDSTHKAPTPEAKSDA
                     GQPTKVGRFQVTTTANKVGRFSVSRTEDKVTELKKEGPVTSPPFRDSEQTVIPAVIPK
                     KEKPELAEPSHLNGPSSDLEAAFLSRGTEDGSGSPHSPPHLCSKSLPVQNLSQSLSNS
                     FNSSYMSSDNESDIEDEDLRLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPA
                     VIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSGTSVLHPQQTLHP
                     AGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVS
                     SQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKF
                     SAPGQLCVPMTSNLGGSTPISAASATSLGHFTKSMCPPQQYGFPPAPFGTQWSGTGGP
                     APQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT"
     exon            1783..1955
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2176
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2334
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2423
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2643
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2977
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3165
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..4401
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            4402..4482
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            4483..4608
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            4609..4893
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            4894..4991
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            4992..5154
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            5155..5271
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            5272..5445
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            5446..5503
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            5504..5626
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            5627..7071
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            7072..7155
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            7156..7239
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            7240..7300
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            7301..7365
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            7366..8027
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            8028..8230
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            8231..8425
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            8426..8613
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            8614..11332
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11307..11312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /note="hexamer: AATAAA"
     polyA_site      11332
                     /gene="Wnk1"
                     /gene_synonym="6430573H23Rik; EG406236; Hsn2; mKIAA0344;
                     Prkwnk1"
                     /note="major polyA site"
ORIGIN      
        1 aaagcggaga gttttcccgg gcggacgcgc tccttctgct cggctattcc gtactccctt
       61 cttcgctgac agaaggcgcc tagtggggtg actgctcttt ttctctccct ctccccctcc
      121 tcccagttct ttcggtctcc tctcctcctg gccagagaag tagcggagcc cgggccccgc
      181 ggtgagcggc cctcccctcc ccacagttcc ctccctcctc tgcccccggc gccggccaga
      241 gaggggaaga ggttcgccag gcccggggcg ggcggggagg cctcggggac ggggggggcc
      301 gctcctcagg cttcgaggct tcgaggcttc ggccctcagc atctagtgat gggcgccctc
      361 tgagactggt cccctctccg gggcgcgtgc tgcctgaggc ggcggccgag cgagcgagcg
      421 agcgagcgag cgaccgggaa cccgggccgc ggccctcctt cccctgcgcg gcccctccca
      481 ctcctgtgcc ccgggctgcc accgcccggc gtcggggctc gtccctcgcg cgctgtgcct
      541 ccgccctcag ggcctatccc gccgtgctcg gcgggcgggc ggcgacggcg gcagcggcag
      601 cggagggagc ttagccgtct tcacttgggt cgcatcctct cgcccaggag ccaattccgc
      661 gccctgctcc tcatgactgc ggcgcttctg ctgccccagc cctaccggcc tccgctcgct
      721 gcaggatgga tgcggactgt gcggcgctaa cccccgtggc tccgctccct cctagcccgc
      781 cgacgagccc gccccgtgag ccgcagcagc ttcggtgcca gcagcagcag ccgccgcagc
      841 agccggactc agtgtcccgc gtcgccctcg gggcgccttg tcagtgctga gtgggagtcg
      901 tccggtggtt cggagcccgc cgtaggagcg ctgcccagca cttcttggtg gcttctccgg
      961 gtcacccttt tcccttttca ttcataaatc cgagcccgca ttcttcactc catcggaccg
     1021 accatgtctg acggtgccgc cgagaagcaa agcggcactc cgggcttcct tacgcctccg
     1081 gcgcccgtcc ccaagaatgg ctcaagctcc gattcttccg tgggcgagaa actgggggct
     1141 acggtcgccg actcgggagt tggcaggacg gaggaatatc ggcgccgccg gcacactatg
     1201 gacaaggaca gtcgtggggc ggccgccacc accaccccca cggagcaccg attcttccgc
     1261 cgaagcgtca tctgcgactc caatgccacc gcgttggagc tgccgggcct ccctctctct
     1321 atcccgcaac ctagtgtacc tgcagtggtg ccccagagtg ctccacccga acctcacaga
     1381 gaggaaactt tgaccgccac cgttgcttcc caggtgtctc agcaaccctc ggctgctgcc
     1441 tcccctgggg aacaagccgt tgtaggctcg gccaccacga ccgtccccag cagtaccagc
     1501 aaagaccgcc cggtgtccca gcccagcctc gtggggagca aagaggagcc accgccgtcc
     1561 agaagtggaa gtggcagtgg tggcgccagc gccaaagagg cgcaggagga taggagccag
     1621 caacaggatg atatcgaaga gctggagacc aaggcagtgg gaatgtccaa tgacggtcgc
     1681 tttctcaaat ttgacatcga aatcggcaga ggctccttta agacagtcta caaaggtctg
     1741 gacaccgaaa ccactgtgga agtcgcctgg tgtgaattgc aggatcgaaa gttaacaaaa
     1801 tctgaaaggc agagattcaa agaagaggct gaaatgttaa agggtcttca acatcccaac
     1861 attgttcgat tctatgattc ctgggaatcc acagtaaaag ggaaaaaatg cattgtttta
     1921 gtgactgaac taatgacatc tggaacactt aagacgtact taaaaaggtt taaagtgatg
     1981 aaaatcaaag ttttaagaag ctggtgtcgg caaatcctta aagggcttca gtttctccac
     2041 acacgaactc caccaattat tcaccgggat cttaaatgtg acaacatctt tatcactggt
     2101 cctactggct cagtcaagat cggagacctt ggtctggcaa ctctaaagcg ggcttctttt
     2161 gccaagagtg tgataggtac cccagagttt atggctcctg agatgtatga ggagaaatat
     2221 gatgaatccg ttgatgttta tgcttttggg atgtgcatgc ttgagatggc tacatctgaa
     2281 tatccatact cagaatgcca aaatgctgcg cagatctacc gtcgagtgac cagtggagtg
     2341 aagccagcca gttttgacaa agtagcaatt cctgaagtaa aagaaattat tgaaggatgt
     2401 atccgacaaa acaaagatga aagatattcc atcaaagacc ttttgaacca tgcctttttc
     2461 caggaggaaa caggggtacg ggtagaatta gcagaagaag atgatggaga aaagatagct
     2521 attaaattat ggctgcgtat tgaagatatt aagaaattaa agggaaaata caaagacaat
     2581 gaagctattg agttttcctt tgacttggag agggatgtac cagaagatgt tgctcaagaa
     2641 atggttgagt ctggttatgt ctgtgaaggt gatcacaaga ccatggctaa agctatcaaa
     2701 gatagggtgt ccttaattaa gagaaaacga gagcagcggc agttggttcg agaagagcaa
     2761 gaaaaaagaa aacaagaaga gagcagtttc aaacagcaga atgaacaaca agcaagcgtt
     2821 tcccaggcag gaatccagca gctctctgct gctagcactg gcatacccac tgctcctgcc
     2881 acctctgctt cagtttctac acaggtagaa cctgaagaac ctgaggcaga tcaacatcaa
     2941 cagctgcaat atcagcaacc tagtatatct gtgttgtctg atggaaccat tgatagtggt
     3001 caaggatcat ccgtattcac agaatccaga gtgagcagcc aacagactgt ttcctatggt
     3061 tcccaacatg agcaggcaca ttctactggc acagcaccag gacatacagt ttctagtatc
     3121 caagcacaat ctcagcccca tggggtgtat ccaccctcaa gtatgcctca gtccatggcg
     3181 catccgtgtg gggggacccc aacataccca gaatcacaga tatttttccc aactattcat
     3241 gaacgtccag tttctttttc accacctccc acctgtccac cgaaagtagc catttcccaa
     3301 cgacgtaaga gcacctcctt cctggaagcc caaactcgcc acttccaacc cctgctgagg
     3361 actgttggcc aaaatcatct tccacctggt agcagcccaa ctaactggac accagaggcc
     3421 atagttatgt tgggtgctac agccaataga gtaaatagag agctatgtga gatgcaggtc
     3481 caacctgtgt ttgagccaac ccagatttac agtgactata gacctggact agtactggca
     3541 gaagaagctc actattttat tcctcaggaa acagtatatc tagctggggt gcattaccag
     3601 gcccaggtgg caggacagta tgagggcatt tcatacaact caccagtact gtcaagtcct
     3661 atgaaacaga tatctgaaca gaagccagtg ccggggggcc ctgcctcaag ttctgtcttt
     3721 gaatttcctt ctggacaggc tttcctggta ggacaccttc agaatttaag attagattct
     3781 ggaccaagtc cagcatcacc cctctctagt atttctgcgc ctaacagtac agatgctaca
     3841 catctgaaat ttcaccctgt ctttgtacct cattctgcac cagctgtgtt aactaatagc
     3901 aatgagaaca gaagcaactg tgtatttgaa tttcatgctc aaacaccaag ttcttcagga
     3961 gaaggaggtg ggattttacc tcaacgtgtt taccgaaatc ggcaggttgc agtggactca
     4021 aatcaggaag aactgtctcc tcagtcagtt ggattacatt gccacctgca gcctgttact
     4081 gaagaacagc gtaataacca tgcccccgaa ttgaccattt ctgtggtaga acctatggga
     4141 caaatctggc caataggaag cccagaatat tccagtgatt cctctcaaat tacttcttca
     4201 gatctcagtg attttcaatc acctccccct acagggggga cagctgcacc ttttggctct
     4261 gacgtctcat tgccctttat tcgcctgcct cagacagtgt tacaagaatc cccactattc
     4321 ttctgtttcc cccaaggaac cacatctcag caggtcttat ctgcttcata ttcttcagga
     4381 ggatctacac tccatccaca ggcacagggg cagaaccagg gccagccatc aagtagtttg
     4441 gctggggttc tatcttcaca acctatccaa catcctcagc agcagggaat acagccaact
     4501 gttccttctc aacaggcagt acagtattca cttccacaag cagcatcttc cagtgaaggt
     4561 actactgcac agccagtgag tcagcctcaa gtgtcagctg gaacacaggg cttcccatct
     4621 cgactgccac cacaataccc aggagattca aatattgctc cctcttccaa cgtggcttct
     4681 gtttgcatcc attctacagt cctagcccct ccttctatgc cgacagaagc attggccacc
     4741 caaggttact ttcctacagt agtgcagcct tatgtggaat caactccttt ggttcctatg
     4801 ggcagtgtag gaggccaggt tcaagtgtcc caaccagcag tgagtttaac gcaacaaccc
     4861 ccaactacat cctcccagca agcagttctg gagagtactc agggagtctc tcaggctgct
     4921 cctccagaac aaactccaat aacacagtca caacctactc agcctgttcc attggttacc
     4981 tctgcagaca gtgcacattc agacgttgct tctggtatga gtgatggcaa tgagaatgct
     5041 ccatcatcca gcgggaggca tgaagggagg acaacaaaac ggcattatag aaaatccgta
     5101 agaagtcgct ctcggcatga aaagacttct cgcccaaaac tgaggatttt aaatgtttca
     5161 aataaaggag accgagtagt agaatgtcaa ttagagactc ataatcggaa gatggttaca
     5221 tttaaatttg acctagatgg tgacaaccct gaagagatag caacaattat ggtgaacaat
     5281 gactttattc tagcaataga gagagagtct tttgtggctc aagtgcggga aattattgaa
     5341 aaggctgatg aaatgttaag tgaggatgtc agtgtggagc ctgagggtga ccagggattg
     5401 gagagtctgc aaggaaagga tgactatggc ttcccaggtt ctcagaaatt agaaggagag
     5461 ttcaagcaac caattgctgt atcttccatg ccacaacaaa taggtgttcc taccagttct
     5521 ttaacgcaag ttgttcattc tgctggaaga cggtttatag tgagtcctgt acctgaaagt
     5581 aggttacgag aatcaaaagt tttcaccagt gacatatcag atccagttgt ggcctctaca
     5641 tctcaggccc ctggaatgaa cttatctcac tctgcttcat ccctcagtct ccagcaggct
     5701 ttttctgaac tgaaacatgg tcagatgact gaaggaccca atacagcacc tccaaatttt
     5761 aatcacatgg cgggaccaac attttctccc tttttggcta gcattgctgg tgtccaaact
     5821 gtagcagcat ccacaccgtc agtatcagtc cctataacaa gcagtcctct taatgacatt
     5881 tccacatcag tgatgcagtc tgagactgca ctgcccactg aaaaaggcat cgtgggagtt
     5941 accactacca gcacaggtgt ggtagcttca ggtggtctca ccacaatgtc tgtgtctgag
     6001 tcaccaacat cttccagtgc ggtttcaagt tctacagtac ctgcagttgt cactgtgtcc
     6061 acaccatccc agccagttca ggcttccaca tctgggagca ttgcttcaag tacaggcagt
     6121 tttcctcctg gaacattttc tacaactaca gctactacaa tgggtagtgt ggtggcccca
     6181 gatgctaagc ctccaactgt attattgcaa caggtagcaa gcaatactgc tggggttgcc
     6241 atagtgacat cagtctctac caccactcca ttcccaggca tggcttcaca gccatccctt
     6301 ccacttagta gcagcacctc tgctcctact ctagctgaaa caatggtggt tagtgcacac
     6361 tcactagaca aggcatctca cagtagtaca gcaggattgg gtttgtcctt ttgtgcgcca
     6421 tcctcttcct cctcctctgg aacagctgtg tctacttctg tttctcagcc tggtatggtt
     6481 catcctttgg tcatctcatc agctgtagtt tctacacctg gcctccccca accagtagta
     6541 cctacttcta cacctttatt accccaagta cctaatatcc cacccttggt acaacctgtt
     6601 gttaatgtgc ctgctgtaca gcaaacactc attcacagtc agcctcaacc agctctgctt
     6661 cccaaccagc ctcacacaca ctgtcctgaa atggatgctg atacccaatc caaagctcct
     6721 gggattgatg atataaagac tctggaggaa aagctgagat ctctctttag tgaacacagc
     6781 tcatctggaa ctcagcatgc ttctgtctcc ctagagacac ctttagtagt agagacgact
     6841 gtcacaccgg gcatcactac cactgctgta gcaccaagta aactcatgac ttccactaca
     6901 agtacttgct taccaccaac cagtttacca ttaggagcag ctggcatgcc agttatgcca
     6961 gtaggcacac ctgggcaggt ttctacccca ggcacacacg cttcagcccc agtcggcact
     7021 gcaacaggag taaaacctgg aacaactccc ccaaagccaa ctaagactgt ggtgccacca
     7081 gtgggtactg aactttcagc cggtactgta ccctgtgagc agctgccacc ttttcctgga
     7141 ccttctctaa ttcagtccca gcaaccacta gaagatcttg atgctcagtt gagaagaaca
     7201 cttagtccag agactattac agtggcacct gcagttggtc ctttgtctac gatgtcttca
     7261 acaacagtta cagaagcagg gactcggctt caaaaggatg gcacagaagg tcatgtcaca
     7321 gcaactagtt caggagctgg tgttgttaag atggggcgat ttcaggtttc agttacaatg
     7381 gatgatgccc agaaagaacg taaaaatagg tcagaagata caaagtcggt tcattttgaa
     7441 tctagcacat cagagtcatc agttctttca agtagtagtc cagagagtac cctggtaaag
     7501 ccagagccta atggaattag tatttctggc atctccttag atgtcccaga cagcacccac
     7561 aaagcaccta ccccagaagc aaagtcagat gcaggacagc ctaccaaggt gggacgtttt
     7621 caggtgacaa ctacagccaa caaagtgggt cgtttctctg tttcaagaac tgaggacaag
     7681 gtcacagagc tcaaaaaaga gggaccagtg acctctcctc ctttcagaga ttcagaacaa
     7741 actgttattc ctgctgtgat accaaagaag gagaagcctg agctggcaga accttcccat
     7801 ttgaatgggc catcttcaga cctggaggct gcctttctaa gtaggggtac agaggatggc
     7861 tcaggtagtc cacactctcc ccctcacctg tgttcaaaga gccttcccgt ccagaatcta
     7921 agtcaaagcc ttagtaattc attcaactcc tcatacatga gtagtgacaa tgagtccgac
     7981 attgaagatg aagacttaag gttagaacta cgacgactac gagaaaaaca tcttaaagag
     8041 attcaggacc tgcagagtcg ccagaagcat gaaattgaat ctttgtatac taaactgggc
     8101 aaggttcccc ctgctgtcat tattccccca gctgctcctc tgtcggggag aagaaggaga
     8161 cccactaaaa gcaaaggcag caagtctagt cgcagcagct cattgggcaa taaaagccca
     8221 cagctttcag gcaacctgtc tggtcagagt ggaacttcag tcttacaccc ccaacagacc
     8281 ctccatcccg caggaaacac ccccgagact gggcacaacc agctgttaca gcctcttaag
     8341 ccatctccct ccagtgacaa cctctattca gcctttacca gtgatggtgc catttcagta
     8401 ccaagccttt ctgctccagg tcaagggacc agcagcacaa ataccgttgg aggaacagtg
     8461 agtagccagg ctgcccaggc tcagcctcct gctatgactt ccagtaggaa gggcacattc
     8521 acagatgacc tgcacaagtt agtagacaac tgggcccgag atgccatgaa tctttcaggc
     8581 aggagaggca gcaaaggaca catgaattat gagggccctg gaatggcaag gaagttctct
     8641 gctcctgggc agctgtgtgt tcccatgacc tcaaacctgg gtggctctac ccccatctct
     8701 gcagcatctg ctacctctct aggtcacttc accaaatcca tgtgtccccc acagcagtat
     8761 ggttttccac ctgccccatt tggcactcag tggagtggaa caggtggtcc agcaccgcag
     8821 ccacttggcc agttccaacc tgtaggaaca gcatccttgc aaaatttcaa catcagcaat
     8881 ttgcagaagt ccatcagcaa ccccccaggt tccaatctac ggaccaccta gtcagcagca
     8941 aggatgttaa ctgagtagat ttgggggcag gaaatggaat gctggggggg tgggggggtg
     9001 gggaagtagc ctatatacta actactagtg ctgcatttaa ctggttattt cttgccagaa
     9061 gggaatgttt ttaataccat tttgagccct cagaatggag aaccaccctc cttctccgtt
     9121 tgttggagtt aaaaaagaag gaatgaacag ctttctggtg gaggggctgc ttgagactgc
     9181 ttttctgttt ttgtacccct cagtaaggcc tagaactaga agagaacctt gttaccagaa
     9241 agccccaaga gctcaatgta agccaaaccc gatctgtatc tgtaagtggg cagagctctt
     9301 taattttgac atgtgtccca aattccttat tccctcaaca atgcacacct taagacaaag
     9361 gttctctccc actctgatcc ctacccccac tttttccccc ccttttacaa cctagtgaaa
     9421 aataccaggg gactggggtt gcaacccttt cttacatagg tcattaatgc tttaagcaaa
     9481 agatattagc agctttgact gcagcattag caattaggga aaacaataac gttccctgcg
     9541 gacatgtaac tttgccatca gttttgatgt ggaaacactg tgatatataa atgttgttgg
     9601 acaacattag ttttaagaga aaaatttgaa aggtttaaaa ggttcacaca cacacaaaag
     9661 ctagctctgt cctttactta ttaagacact ttaacttttt cctttgtatt tccattgtat
     9721 tagataaata aatgtgaatg taaaattgta taaattactg tacttgaata cttctgttct
     9781 cccagtattg cttgctggat attttagtgc cttggacttc tattgcttct gctgttagca
     9841 tcaacttacc agaccccagg tcaccaaagg gtatgtggaa agaaatctta ggtccacatg
     9901 accccagcag tggatatgcc aaagggaaaa tgaaagtgtt tttggttttg gtttggttta
     9961 ttattttgtt ttgttttgtt ttgttttctc agtcgtttca attttgccta gagcaggtgt
    10021 aaggtgagca cagtgaagtt ggcatcagtg gttgaaaaca aagttctgtt tcagaaagaa
    10081 tgaggagggt tgtgagctgt gtgtgaaaga taaggactga agatgtgggg acaagcttgt
    10141 ttgaagtgat gcttggccag tgaacaaacg agatctagaa gagattaaac aaaaagaggg
    10201 gagtgggcta gattttaagt tgggaaggat gacagtgcca agtcacagat ggaaaatgtt
    10261 gctctaagga aacctgctcg gttgccccca cctcttgttg cacaagtacc accacacttt
    10321 ctctgcttta agtactgtat tgtgggaatt caacccactt ctaactttct aagcattgta
    10381 ggaaaaagtg cttatttcct ttcctagctg tccaaatggt tgcacagtca gaatctattg
    10441 tgctgcctgt acttctatat tagagctaat actctcacat tttcataccc atatccccaa
    10501 ataacagcaa ccaaccagta ttatctgatt ttctggcaat agggacctaa ttggctgttt
    10561 gggatctgaa tattctggaa aagtggaaaa agtagaactc atttcttggg tgcttcctcc
    10621 ttcaccttga gttgggaact gacacatttc caactttatg acacatccca ggagttgcca
    10681 cactagcaag gcttcttcct gtaagagtct tgttcttttt aagttggtcg aggcccaaag
    10741 atacaagtga gagctgaaag tttcaggaga gggaagtgta atgagcaggg cttttgtttc
    10801 aggtcgtgct accaaagtgt tgactcattt attccttttg ggagcggtaa gaggtactgt
    10861 gggagtgaag caggttacag gatgactgag tgcttcatac cagcagtaga gggagccaaa
    10921 tgcagcatgt ggtgtttggt ttagtcattc tgagtgaggt ctgttccccc ttcacagagg
    10981 tttacttact gttggtttcc ccctcctgtg gatgaaataa ctgaagccta gagaaacgag
    11041 ctagtgctac tgaactgttt aaattatttt tgtgttaata gtacactttg agtatctttc
    11101 cacatttaaa aagaactttc cgaattataa atgttttcct tacgttattt aacaatgtac
    11161 actgtttaaa actgaattca aaccttgggg tttctcagca gccgttacct gtacatttgc
    11221 actaactccg ggggctgcgc ttctgtaaga ttgcgctttg tgcttcagtt tgttaccttt
    11281 gtagacttat ttaatgaaac cattcaaata aaccaaactt gcttttgttg aaaaaaaaaa
    11341 aaaaaa
//
