LOCUS       NM_026582               5485 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus wntless WNT ligand secretion mediator (Wls),
            transcript variant 1, mRNA.
ACCESSION   NM_026582
VERSION     NM_026582.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ferrena,A., Zhang,X., Shrestha,R., Zheng,D. and Liu,W.
  TITLE     Six3 and Six6 jointly control diverse target genes in multiple cell
            populations over developmental trajectories of mouse embryonic
            retinal progenitor cells
  JOURNAL   PLoS One 19 (10), e0308839 (2024)
   PUBMED   39446806
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Polsani,N., Yung,T., Thomas,E., Phung-Rojas,M., Gupta,I.,
            Denker,J., Lau,K., Feng,X., Ibarra,B., Hopyan,S. and Atit,R.P.
  TITLE     Mesenchymal Wnts are required for morphogenetic movements of
            calvarial osteoblasts during apical expansion
  JOURNAL   Development 151 (12) (2024)
   PUBMED   38814743
REFERENCE   3  (bases 1 to 5485)
  AUTHORS   Gupta,I., Yeung,J., Rahimi-Balaei,M., Wu,S.R. and Goldowitz,D.
  TITLE     Msx genes delineate a novel molecular map of the developing
            cerebellar neuroepithelium
  JOURNAL   Front Mol Neurosci 17, 1356544 (2024)
   PUBMED   38742226
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5485)
  AUTHORS   Tian,Y., Chen,J., Huang,W., Ren,Q., Feng,J., Liao,J., Fu,H.,
            Zhou,L. and Liu,Y.
  TITLE     Myeloid-derived Wnts play an indispensible role in macrophage and
            fibroblast activation and kidney fibrosis
  JOURNAL   Int J Biol Sci 20 (6), 2310-2322 (2024)
   PUBMED   38617540
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5485)
  AUTHORS   Russell,N.X., Burra,K., Shah,R.M., Bottasso-Arias,N.,
            Mohanakrishnan,M., Snowball,J., Ediga,H.H., Madala,S.K. and
            Sinner,D.
  TITLE     Wnt signaling regulates ion channel expression to promote smooth
            muscle and cartilage formation in developing mouse trachea
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 325 (6), L788-L802 (2023)
   PUBMED   37873566
REFERENCE   6  (bases 1 to 5485)
  AUTHORS   Jin,J., Kittanakom,S., Wong,V., Reyes,B.A., Van Bockstaele,E.J.,
            Stagljar,I., Berrettini,W. and Levenson,R.
  TITLE     Interaction of the mu-opioid receptor with GPR177 (Wntless)
            inhibits Wnt secretion: potential implications for opioid
            dependence
  JOURNAL   BMC Neurosci 11, 33 (2010)
   PUBMED   20214800
  REMARK    GeneRIF: These findings suggest a previously unrecognized role for
            GPR177 in regulating cellular response to opioid agonist drugs, and
            could create new avenues of investigation regarding the
            pharmacotherapy of drug dependence.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5485)
  AUTHORS   Fu,J., Jiang,M., Mirando,A.J., Yu,H.M. and Hsu,W.
  TITLE     Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required
            for embryonic axis formation
  JOURNAL   Proc Natl Acad Sci U S A 106 (44), 18598-18603 (2009)
   PUBMED   19841259
  REMARK    GeneRIF: A reciprocal regulation of Wnt and Gpr177 is essential for
            the patterning of the anterior-posterior axis during mammalian
            development.
REFERENCE   8  (bases 1 to 5485)
  AUTHORS   Nguyen,C., Singson,E., Kim,J.Y., Cornelius,J.G., Attia,R.,
            Doyle,M.E., Bulosan,M., Cha,S. and Peck,A.B.
  TITLE     Sjogren's syndrome-like disease of C57BL/6.NOD-Aec1 Aec2 mice:
            gender differences in keratoconjunctivitis sicca defined by a
            cross-over in the chromosome 3 Aec1 locus
  JOURNAL   Scand J Immunol 64 (3), 295-307 (2006)
   PUBMED   16918699
REFERENCE   9  (bases 1 to 5485)
  AUTHORS   Bartscherer,K., Pelte,N., Ingelfinger,D. and Boutros,M.
  TITLE     Secretion of Wnt ligands requires Evi, a conserved transmembrane
            protein
  JOURNAL   Cell 125 (3), 523-533 (2006)
   PUBMED   16678096
REFERENCE   10 (bases 1 to 5485)
  AUTHORS   Neophytou,P.I., Muir,E.M. and Hutton,J.C.
  TITLE     A subtractive cloning approach to the identification of mRNAs
            specifically expressed in pancreatic beta-cells
  JOURNAL   Diabetes 45 (2), 127-133 (1996)
   PUBMED   8549854
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121906.3, BC075615.1 and AC225719.3.
            
            On Sep 30, 2017 this sequence version replaced NM_026582.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.5956.1, AK029053.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AC121906.3         33075-33119         c
            46-2636             BC075615.1         1-2591
            2637-5485           AC225719.3         34062-36910
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 82.65 cM"
     gene            1..5485
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="wntless WNT ligand secretion mediator"
                     /db_xref="GeneID:68151"
                     /db_xref="MGI:MGI:1915401"
     exon            1..398
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     CDS             293..1918
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="isoform 1 is encoded by transcript variant 1;
                     integral membrane protein GPR177; protein evenness
                     interrupted homolog; G protein-coupled receptor 177;
                     wntless homolog"
                     /codon_start=1
                     /product="protein wntless homolog isoform 1"
                     /protein_id="NP_080858.3"
                     /db_xref="CCDS:CCDS38684.1"
                     /db_xref="GeneID:68151"
                     /db_xref="MGI:MGI:1915401"
                     /translation="MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVP
                     YTAIKCVDVRKNHHKTRWLAPWGPNKCDKIRDIEEAIPREIEANDIVFSVHIPLPSME
                     MSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDMFSEWTEMAHERVPRKL
                     KCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKD
                     IRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGIS
                     MTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAG
                     YWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLY
                     FLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFF
                     IVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDL
                     GVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE"
     exon            399..671
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            672..796
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            797..958
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1095
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1264
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1362
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1426
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1570
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1654
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1808
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..5485
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2601..2606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: ATTAAA"
     polyA_site      2636
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="major polyA site"
     regulatory      2711..2716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: AATAAA"
     polyA_site      2733
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
     regulatory      3548..3553
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: AATAAA"
     regulatory      3561..3566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: ATTATA"
     polyA_site      3582
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
     polyA_site      3587
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
ORIGIN      
        1 gagagggagg gaggaagaaa gagagggagg cgtgtcatgt gatccgcttc cctgcttctt
       61 taagcgtcca ctggctgccg ggcggccgca gtctcagctc tgggcatctg gggagcccaa
      121 gcggcttcgc ccaggcatcc gcgcgggcgc ctgcggtctg tcccacctcc tctgctaacg
      181 cacccttgaa gttttgcagc accaggaaaa gagggcaagg aagaaggaga ggggaagcga
      241 aagcataccc taaaacattt acttaaagga gaaaagaaag ggggtcgcag aaatggctgg
      301 ggcaattata gaaaacatga gtaccaagaa gctctgcatt gttggaggga ttcttctggt
      361 tttccaaatc gttgcctttc tggtgggagg cttgatcgct ccagcaccca caacagcagt
      421 accctacacg gcaataaaat gtgtggatgt ccgtaagaac caccataaaa caagatggct
      481 ggcgccttgg ggacctaaca agtgtgacaa gatccgtgac atcgaggaag caattccaag
      541 ggaaattgaa gcaaatgaca ttgtgttttc tgtacacatt cccctccctt ctatggagat
      601 gagtccatgg ttccagttta tgctgtttat cttgcagcta gacattgctt tcaagctgaa
      661 caaccaaatc agagaaaatg cagaaatttc catggatgtt tccctgggtt accgtgatga
      721 tatgttttct gaatggactg aaatggccca tgaaagagta ccacgtaaac tcaaatgtac
      781 ttttacatcc cccaagaccc cagagcacga aggtcgttat tataattgtg atgtccttcc
      841 tttcatggaa attgggtcag tggctcataa gtattacctt ctaaatatcc ggctacctgt
      901 aaatgagaaa aagaaaatca atgttggaat tggggagata aaggacattc ggttggtggg
      961 aatccaccaa aacggaggtt tcactaaggt atggtttgct atgaagacct tccttacacc
     1021 cagcatcttc atcattatgg tgtggtattg gaggaggatc accatgatgt cccgacctcc
     1081 agtgcttctg gaaaaagtca tctttgccct tgggatttcc atgaccttta tcaatatccc
     1141 tgtggaatgg ttttctattg gatttgactg gacctggatg ctgttatttg gtgacatccg
     1201 acagggcatc ttctatgcaa tgcttctttc tttctggatc atcttctgtg gcgagcacat
     1261 gatggatcaa catgaacgga atcacattgc agggtactgg aagcaagttg gaccaattgc
     1321 tgttggctcc ttctgcctct tcatatttga catgtgtgaa agaggagtac aactcacaaa
     1381 tcctttctac agtatctgga ctacagatgt tggaacagaa ctggctatgg ctttcatcat
     1441 tgtggcaggt atctgcctct gcctctattt cctgtttctg tgcttcatgg tatttcaggt
     1501 atttagaaac atcagtggga agcagtctag cctcccagcc atgagcaaag tccggaggct
     1561 gcactatgag ggtctgatct tcaggttcaa gttcctcatg cttatcacct tggcttgtgc
     1621 tgccatgact gttatcttct tcatcgttag tcaggtgtca gaaggccatt ggaaatgggg
     1681 tggtgtcaca gttcaagtga gcagtgcttt cttcactgga atctatggga tgtggaacct
     1741 gtatgtcttt gctttgatgt tcttgtatgc accatcccat aagaactatg gggaagacca
     1801 gtctaatggt gacctgggtg tccacagcgg ggaagaactg cagcttacta ccactataac
     1861 ccatgtagat ggaccaactg agatctacaa gttgacccgt aaagaagcac aggaatagga
     1921 ggctatggta tggcactgag ctggaattgt ctctctatcc agcctcttcc cctggagtag
     1981 agatcctact atacagagct cagtgaaaag tggataccta gtgcttctta ggtatagttt
     2041 cttaaaacaa cagatggaca tttgtcagat gacctccaat ggtggctgtt gcaaaacttc
     2101 tgccactact atatagcaca tgttaacaaa aactgatatg acacatttcc tgtgatcatt
     2161 gttaattagt gacatagcag catctgtagc aggtgggtta gtaaccctca catagagggg
     2221 tatactcctt gggatggttt gggccaatag gggctatatt gagtagtttg tgactttttt
     2281 ctgcttttga ttctgggata tgtttaaaca ttctttgcag tccaacattt gctgatgtca
     2341 tagtcttttt actctcctcc atgggcactt ttttctattt atatcaccaa gtagccaaat
     2401 gagttattta agttgtcagt acaaatatgt caacttttta aaaaatggat tactataaaa
     2461 taaccaaact tcatgagaaa ttttatctta ccagaggcat ttcagctttg gaaccaaatc
     2521 tgtgtatttg atactaatct gtctgtttgt ttgtggattt tgagaaatat ttgcttaact
     2581 acccaaatag gatttctggt attaaatggc ctttgggtcc taaaagcttt ttttaacttc
     2641 ttgcttaaaa tgtgttttct tttgataaga ttcttcaaac aatctccaaa agtgtaaatc
     2701 taatcatgtg aataaaatga atgtaaatat gcaccccttc ctgtcccaac agttctcagg
     2761 catacagatt caaatctgct tggatcttgg agtacccagg tgctggcaga aatagcttga
     2821 gtatttctgg aggtgaaggg ataccttctg tatcaagtat taagccattc taggccagca
     2881 aggaatgctc acatacatga ggaatcagat gctttatacc cgcatacaca gctctcattt
     2941 cagtacccct aatagcctat gacattgctt ttgtttgttt gctttttatt ttatttgtgc
     3001 cttttttaga tgctaaagaa gccacatgac atagcagata aaatactcaa ttctgaattt
     3061 aaaaggccgt tttctatcga aggtctatca tgctctagtt gtgtgatgtt gttcaagcta
     3121 tctgacataa ctattcccca aattacaagc taacattcat aaagtgtaac tgtcttcagg
     3181 gtcacaatga aaaaataatt aatgtatcag ttatacatcc gtattgatta actttgcagt
     3241 ctaaaaaaaa aaattccatg agagtctcat tgtttaggta gcaatgattg aaacaaagtt
     3301 tgtggtatat tctgtttaat taacctacat ggcaaacccc atcctgtgtt tttcctaact
     3361 ttggccagaa ccatggtaaa cagtgacaga gaaaacactt aaaggttgga tcttgtcttt
     3421 atgccttttt ctaaactggt gaatgttaga tgggtatgtg atccattgta cccaacatcc
     3481 acttactgat ctgccaccta agtataaaga atcaagtgta gcctgtgctt ctgtttctat
     3541 tagagcaaat aaacttcaac attatagtta tctccccttc tagaaaatgg agtgaggtgt
     3601 ctctattctt tgggactgga taatactctg aaagaatatt aaagtattat tcttcccagg
     3661 accaaatgat agagagtaaa gtattagaaa agccatagtg gttatgtaat ttataggagg
     3721 tttgggtaaa gttaggtgcc aaaacacaag atgagcctcc atgcccttgg agccacaata
     3781 aatatgattt caatgtcaca gtaagagtaa ccagcctgaa gccgggcgtg gtggcgcacg
     3841 cctttaatcc cagcactcgg gagttcgagg ccagcctggt ctacaaagtg agttccagga
     3901 cagccagggc tatacagaga aaccctgtct tgaaaaacca aaaaaagaaa aaagttacca
     3961 gtctggctca aatttataca aagaagtgat atgggtagaa ttacctctga agctaggaaa
     4021 gggttaatgg atccagcaat gagttcatag gccagagcca gtcagcagca aaggatgtgg
     4081 agaagggtgg attattttgg agataaaaca gagcagacaa ggtttgaatg ttatgaatgt
     4141 gaacttcata aagaggcaaa gagcagagtc aaatttcaga cccttgctga gaagtggact
     4201 agcaggtgcc tggctcaagt aaagattcct gaatgaaatg tcaatatgtc cataaataga
     4261 aaaagccaag attagctcaa aaaaaggacc ttcctagaat agtttatggt tcccttcaaa
     4321 agttgcacta tcccagaaag aataggcttg gggatatctc taatgggact caacttcagg
     4381 agctcatgtt taccccaact ttcatttaca agtctctctc atcaaatcta aagacaatat
     4441 gtcctagtgc caatccaatt acacagtgag tcatgtcaaa cagcatgacc ttgtgaatag
     4501 cagtcagtct gcaaaggaag gagaaccctt tgagctgggc aaacctttga aaaacatcta
     4561 acatgatggg gatgcggaag aattcatatt tgtctagact gagttttaac tattacttcc
     4621 tggagggaac tatgaatagg gtgttgatgg cagactttgc catcaacaat tcttattaaa
     4681 aatgagaagt atgcctgggc agagcatcaa aacattaata atgtgtagcc aggtatggcc
     4741 acattcagta ttagagaaaa atgagaaagg ttaagaaata acatgtggtt ttcaaagaag
     4801 tcaaaagcca gagtgataca aacagggcag aacttagctg aggccggaat gctttatata
     4861 agtgactgga gcataatgga tgtggtttgg ggtatgtgtg acattcgtat gtatcagggg
     4921 aaaagttgga cagagaagca gagcagagtc aatggataga catgctggtc aacaagaaga
     4981 aagacggttg ttttgaatgg agccagaata aaaacactga ctttgaaatc tgagaccagg
     5041 tattaaggaa agacaaggat acaatgaatc aacaccctaa gaataccagt gatttggatc
     5101 tagaaacaag ctgaggactt cactctgaag gattcaaagt acaatttcat tgcacacagt
     5161 agagaaattc tcagtggaca gtggaagaga aaattgagtt tcaaagaaga aagtttacaa
     5221 ccctgggggt ccaccaagat aagcctagcc agctccaagt atccaagaaa tgcctcagaa
     5281 agagagaaaa ctatgagaga tgatcagatc tgtggaaaaa atagctatga gtacctgttt
     5341 tgcatacaca taactgctaa aataaatctt acaaaatcta aaggatattt cagaaaagat
     5401 tacagttgtc tatctataaa ataaaattca ccaccagtat gttcaacaaa tacatagaaa
     5461 ataaaatcac tttcatttgg ctacc
//
