LOCUS       NM_001356349            5566 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus wntless WNT ligand secretion mediator (Wls),
            transcript variant 2, mRNA.
ACCESSION   NM_001356349 NR_037590
VERSION     NM_001356349.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ferrena,A., Zhang,X., Shrestha,R., Zheng,D. and Liu,W.
  TITLE     Six3 and Six6 jointly control diverse target genes in multiple cell
            populations over developmental trajectories of mouse embryonic
            retinal progenitor cells
  JOURNAL   PLoS One 19 (10), e0308839 (2024)
   PUBMED   39446806
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Polsani,N., Yung,T., Thomas,E., Phung-Rojas,M., Gupta,I.,
            Denker,J., Lau,K., Feng,X., Ibarra,B., Hopyan,S. and Atit,R.P.
  TITLE     Mesenchymal Wnts are required for morphogenetic movements of
            calvarial osteoblasts during apical expansion
  JOURNAL   Development 151 (12) (2024)
   PUBMED   38814743
REFERENCE   3  (bases 1 to 5566)
  AUTHORS   Gupta,I., Yeung,J., Rahimi-Balaei,M., Wu,S.R. and Goldowitz,D.
  TITLE     Msx genes delineate a novel molecular map of the developing
            cerebellar neuroepithelium
  JOURNAL   Front Mol Neurosci 17, 1356544 (2024)
   PUBMED   38742226
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5566)
  AUTHORS   Tian,Y., Chen,J., Huang,W., Ren,Q., Feng,J., Liao,J., Fu,H.,
            Zhou,L. and Liu,Y.
  TITLE     Myeloid-derived Wnts play an indispensible role in macrophage and
            fibroblast activation and kidney fibrosis
  JOURNAL   Int J Biol Sci 20 (6), 2310-2322 (2024)
   PUBMED   38617540
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5566)
  AUTHORS   Russell,N.X., Burra,K., Shah,R.M., Bottasso-Arias,N.,
            Mohanakrishnan,M., Snowball,J., Ediga,H.H., Madala,S.K. and
            Sinner,D.
  TITLE     Wnt signaling regulates ion channel expression to promote smooth
            muscle and cartilage formation in developing mouse trachea
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 325 (6), L788-L802 (2023)
   PUBMED   37873566
REFERENCE   6  (bases 1 to 5566)
  AUTHORS   Jin,J., Kittanakom,S., Wong,V., Reyes,B.A., Van Bockstaele,E.J.,
            Stagljar,I., Berrettini,W. and Levenson,R.
  TITLE     Interaction of the mu-opioid receptor with GPR177 (Wntless)
            inhibits Wnt secretion: potential implications for opioid
            dependence
  JOURNAL   BMC Neurosci 11, 33 (2010)
   PUBMED   20214800
  REMARK    GeneRIF: These findings suggest a previously unrecognized role for
            GPR177 in regulating cellular response to opioid agonist drugs, and
            could create new avenues of investigation regarding the
            pharmacotherapy of drug dependence.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5566)
  AUTHORS   Fu,J., Jiang,M., Mirando,A.J., Yu,H.M. and Hsu,W.
  TITLE     Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required
            for embryonic axis formation
  JOURNAL   Proc Natl Acad Sci U S A 106 (44), 18598-18603 (2009)
   PUBMED   19841259
  REMARK    GeneRIF: A reciprocal regulation of Wnt and Gpr177 is essential for
            the patterning of the anterior-posterior axis during mammalian
            development.
REFERENCE   8  (bases 1 to 5566)
  AUTHORS   Nguyen,C., Singson,E., Kim,J.Y., Cornelius,J.G., Attia,R.,
            Doyle,M.E., Bulosan,M., Cha,S. and Peck,A.B.
  TITLE     Sjogren's syndrome-like disease of C57BL/6.NOD-Aec1 Aec2 mice:
            gender differences in keratoconjunctivitis sicca defined by a
            cross-over in the chromosome 3 Aec1 locus
  JOURNAL   Scand J Immunol 64 (3), 295-307 (2006)
   PUBMED   16918699
REFERENCE   9  (bases 1 to 5566)
  AUTHORS   Bartscherer,K., Pelte,N., Ingelfinger,D. and Boutros,M.
  TITLE     Secretion of Wnt ligands requires Evi, a conserved transmembrane
            protein
  JOURNAL   Cell 125 (3), 523-533 (2006)
   PUBMED   16678096
REFERENCE   10 (bases 1 to 5566)
  AUTHORS   Neophytou,P.I., Muir,E.M. and Hutton,J.C.
  TITLE     A subtractive cloning approach to the identification of mRNAs
            specifically expressed in pancreatic beta-cells
  JOURNAL   Diabetes 45 (2), 127-133 (1996)
   PUBMED   8549854
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121906.3, BQ179316.1, AK019886.1 and AC225719.3.
            
            On Sep 30, 2017 this sequence version replaced NR_037590.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019886.1, SRR7345562.1510840.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AC121906.3         33075-33119         c
            46-89               BQ179316.1         1-44
            90-201              AK019886.1         2-113
            202-2425            AK019886.1         115-2338
            2426-5566           AC225719.3         33770-36910
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 82.65 cM"
     gene            1..5566
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="wntless WNT ligand secretion mediator"
                     /db_xref="GeneID:68151"
                     /db_xref="MGI:MGI:1915401"
     exon            1..398
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            399..479
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            480..752
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     CDS             674..1999
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; integral membrane protein GPR177; protein
                     evenness interrupted homolog; G protein-coupled receptor
                     177; wntless homolog"
                     /codon_start=1
                     /product="protein wntless homolog isoform 2 precursor"
                     /protein_id="NP_001343278.1"
                     /db_xref="GeneID:68151"
                     /db_xref="MGI:MGI:1915401"
                     /translation="MEMSPWFQFMLFILQLDIAFKLNNQIRENAEISMDVSLGYRDDM
                     FSEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYNCDVLPFMEIGSVAHKYYLLNIRLP
                     VNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMS
                     RPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIF
                     CGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTE
                     LAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKF
                     LMLITLACAAMTVIFFIVSQVSEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLY
                     APSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE"
     exon            753..877
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1039
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1176
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1345
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1443
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1507
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1651
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1735
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1889
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..5566
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2682..2687
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: ATTAAA"
     polyA_site      2717
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="major polyA site"
     regulatory      2792..2797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: AATAAA"
     polyA_site      2814
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
     regulatory      3629..3634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: AATAAA"
     regulatory      3642..3647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
                     /note="hexamer: ATTATA"
     polyA_site      3663
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
     polyA_site      3668
                     /gene="Wls"
                     /gene_synonym="5031439A09Rik; EVI; Gpr177"
ORIGIN      
        1 gagagggagg gaggaagaaa gagagggagg cgtgtcatgt gatccgcttc cctgcttctt
       61 taagcgtcca ctggctgccg ggcggccgca gtctcagctc tgggcatctg gggagcccaa
      121 gcggcttcgc ccaggcatcc gcgcgggcgc ctgcggtctg tcccacctcc tctgctaacg
      181 cacccttgaa gttttgcagc accaggaaaa gagggcaagg aagaaggaga ggggaagcga
      241 aagcataccc taaaacattt acttaaagga gaaaagaaag ggggtcgcag aaatggctgg
      301 ggcaattata gaaaacatga gtaccaagaa gctctgcatt gttggaggga ttcttctggt
      361 tttccaaatc gttgcctttc tggtgggagg cttgatcgga ggctaaagca gaaggatcac
      421 tgttacttgg ggagtgactt caaggccagc ttcaacaact tagtgggacc ctgtctcaac
      481 tccagcaccc acaacagcag taccctacac ggcaataaaa tgtgtggatg tccgtaagaa
      541 ccaccataaa acaagatggc tggcgccttg gggacctaac aagtgtgaca agatccgtga
      601 catcgaggaa gcaattccaa gggaaattga agcaaatgac attgtgtttt ctgtacacat
      661 tcccctccct tctatggaga tgagtccatg gttccagttt atgctgttta tcttgcagct
      721 agacattgct ttcaagctga acaaccaaat cagagaaaat gcagaaattt ccatggatgt
      781 ttccctgggt taccgtgatg atatgttttc tgaatggact gaaatggccc atgaaagagt
      841 accacgtaaa ctcaaatgta cttttacatc ccccaagacc ccagagcacg aaggtcgtta
      901 ttataattgt gatgtccttc ctttcatgga aattgggtca gtggctcata agtattacct
      961 tctaaatatc cggctacctg taaatgagaa aaagaaaatc aatgttggaa ttggggagat
     1021 aaaggacatt cggttggtgg gaatccacca aaacggaggt ttcactaagg tatggtttgc
     1081 tatgaagacc ttccttacac ccagcatctt catcattatg gtgtggtatt ggaggaggat
     1141 caccatgatg tcccgacctc cagtgcttct ggaaaaagtc atctttgccc ttgggatttc
     1201 catgaccttt atcaatatcc ctgtggaatg gttttctatt ggatttgact ggacctggat
     1261 gctgttattt ggtgacatcc gacagggcat cttctatgca atgcttcttt ctttctggat
     1321 catcttctgt ggcgagcaca tgatggatca acatgaacgg aatcacattg cagggtactg
     1381 gaagcaagtt ggaccaattg ctgttggctc cttctgcctc ttcatatttg acatgtgtga
     1441 aagaggagta caactcacaa atcctttcta cagtatctgg actacagatg ttggaacaga
     1501 actggctatg gctttcatca ttgtggcagg tatctgcctc tgcctctatt tcctgtttct
     1561 gtgcttcatg gtatttcagg tatttagaaa catcagtggg aagcagtcta gcctcccagc
     1621 catgagcaaa gtccggaggc tgcactatga gggtctgatc ttcaggttca agttcctcat
     1681 gcttatcacc ttggcttgtg ctgccatgac tgttatcttc ttcatcgtta gtcaggtgtc
     1741 agaaggccat tggaaatggg gtggtgtcac agttcaagtg agcagtgctt tcttcactgg
     1801 aatctatggg atgtggaacc tgtatgtctt tgctttgatg ttcttgtatg caccatccca
     1861 taagaactat ggggaagacc agtctaatgg tgacctgggt gtccacagcg gggaagaact
     1921 gcagcttact accactataa cccatgtaga tggaccaact gagatctaca agttgacccg
     1981 taaagaagca caggaatagg aggctatggt atggcactga gctggaattg tctctctatc
     2041 cagcctcttc ccctggagta gagatcctac tatacagagc tcagtgaaaa gtggatacct
     2101 agtgcttctt aggtatagtt tcttaaaaca acagatggac atttgtcaga tgacctccaa
     2161 tggtggctgt tgcaaaactt ctgccactac tatatagcac atgttaacaa aaactgatat
     2221 gacacatttc ctgtgatcat tgttaattag tgacatagca gcatctgtag caggtgggtt
     2281 agtaaccctc acatagaggg gtatactcct tgggatggtt tgggccaata ggggctatat
     2341 tgagtagttt gtgacttttt tctgcttttg attctgggat atgtttaaac attctttgca
     2401 gtccaacatt tgctgatgtc atagtctttt tactctcctc catgggcact tttttctatt
     2461 tatatcacca agtagccaaa tgagttattt aagttgtcag tacaaatatg tcaacttttt
     2521 aaaaaatgga ttactataaa ataaccaaac ttcatgagaa attttatctt accagaggca
     2581 tttcagcttt ggaaccaaat ctgtgtattt gatactaatc tgtctgtttg tttgtggatt
     2641 ttgagaaata tttgcttaac tacccaaata ggatttctgg tattaaatgg cctttgggtc
     2701 ctaaaagctt tttttaactt cttgcttaaa atgtgttttc ttttgataag attcttcaaa
     2761 caatctccaa aagtgtaaat ctaatcatgt gaataaaatg aatgtaaata tgcacccctt
     2821 cctgtcccaa cagttctcag gcatacagat tcaaatctgc ttggatcttg gagtacccag
     2881 gtgctggcag aaatagcttg agtatttctg gaggtgaagg gataccttct gtatcaagta
     2941 ttaagccatt ctaggccagc aaggaatgct cacatacatg aggaatcaga tgctttatac
     3001 ccgcatacac agctctcatt tcagtacccc taatagccta tgacattgct tttgtttgtt
     3061 tgctttttat tttatttgtg ccttttttag atgctaaaga agccacatga catagcagat
     3121 aaaatactca attctgaatt taaaaggccg ttttctatcg aaggtctatc atgctctagt
     3181 tgtgtgatgt tgttcaagct atctgacata actattcccc aaattacaag ctaacattca
     3241 taaagtgtaa ctgtcttcag ggtcacaatg aaaaaataat taatgtatca gttatacatc
     3301 cgtattgatt aactttgcag tctaaaaaaa aaaattccat gagagtctca ttgtttaggt
     3361 agcaatgatt gaaacaaagt ttgtggtata ttctgtttaa ttaacctaca tggcaaaccc
     3421 catcctgtgt ttttcctaac tttggccaga accatggtaa acagtgacag agaaaacact
     3481 taaaggttgg atcttgtctt tatgcctttt tctaaactgg tgaatgttag atgggtatgt
     3541 gatccattgt acccaacatc cacttactga tctgccacct aagtataaag aatcaagtgt
     3601 agcctgtgct tctgtttcta ttagagcaaa taaacttcaa cattatagtt atctcccctt
     3661 ctagaaaatg gagtgaggtg tctctattct ttgggactgg ataatactct gaaagaatat
     3721 taaagtatta ttcttcccag gaccaaatga tagagagtaa agtattagaa aagccatagt
     3781 ggttatgtaa tttataggag gtttgggtaa agttaggtgc caaaacacaa gatgagcctc
     3841 catgcccttg gagccacaat aaatatgatt tcaatgtcac agtaagagta accagcctga
     3901 agccgggcgt ggtggcgcac gcctttaatc ccagcactcg ggagttcgag gccagcctgg
     3961 tctacaaagt gagttccagg acagccaggg ctatacagag aaaccctgtc ttgaaaaacc
     4021 aaaaaaagaa aaaagttacc agtctggctc aaatttatac aaagaagtga tatgggtaga
     4081 attacctctg aagctaggaa agggttaatg gatccagcaa tgagttcata ggccagagcc
     4141 agtcagcagc aaaggatgtg gagaagggtg gattattttg gagataaaac agagcagaca
     4201 aggtttgaat gttatgaatg tgaacttcat aaagaggcaa agagcagagt caaatttcag
     4261 acccttgctg agaagtggac tagcaggtgc ctggctcaag taaagattcc tgaatgaaat
     4321 gtcaatatgt ccataaatag aaaaagccaa gattagctca aaaaaaggac cttcctagaa
     4381 tagtttatgg ttcccttcaa aagttgcact atcccagaaa gaataggctt ggggatatct
     4441 ctaatgggac tcaacttcag gagctcatgt ttaccccaac tttcatttac aagtctctct
     4501 catcaaatct aaagacaata tgtcctagtg ccaatccaat tacacagtga gtcatgtcaa
     4561 acagcatgac cttgtgaata gcagtcagtc tgcaaaggaa ggagaaccct ttgagctggg
     4621 caaacctttg aaaaacatct aacatgatgg ggatgcggaa gaattcatat ttgtctagac
     4681 tgagttttaa ctattacttc ctggagggaa ctatgaatag ggtgttgatg gcagactttg
     4741 ccatcaacaa ttcttattaa aaatgagaag tatgcctggg cagagcatca aaacattaat
     4801 aatgtgtagc caggtatggc cacattcagt attagagaaa aatgagaaag gttaagaaat
     4861 aacatgtggt tttcaaagaa gtcaaaagcc agagtgatac aaacagggca gaacttagct
     4921 gaggccggaa tgctttatat aagtgactgg agcataatgg atgtggtttg gggtatgtgt
     4981 gacattcgta tgtatcaggg gaaaagttgg acagagaagc agagcagagt caatggatag
     5041 acatgctggt caacaagaag aaagacggtt gttttgaatg gagccagaat aaaaacactg
     5101 actttgaaat ctgagaccag gtattaagga aagacaagga tacaatgaat caacacccta
     5161 agaataccag tgatttggat ctagaaacaa gctgaggact tcactctgaa ggattcaaag
     5221 tacaatttca ttgcacacag tagagaaatt ctcagtggac agtggaagag aaaattgagt
     5281 ttcaaagaag aaagtttaca accctggggg tccaccaaga taagcctagc cagctccaag
     5341 tatccaagaa atgcctcaga aagagagaaa actatgagag atgatcagat ctgtggaaaa
     5401 aatagctatg agtacctgtt ttgcatacac ataactgcta aaataaatct tacaaaatct
     5461 aaaggatatt tcagaaaaga ttacagttgt ctatctataa aataaaattc accaccagta
     5521 tgttcaacaa atacatagaa aataaaatca ctttcatttg gctacc
//
