LOCUS       NM_178398               3942 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus WD repeat domain, phosphoinositide interacting 2
            (Wipi2), transcript variant 1, mRNA.
ACCESSION   NM_178398
VERSION     NM_178398.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Zhao,Y., Zou,Z., Sun,D., Li,Y., Sinha,S.C., Yu,L., Bennett,L. and
            Levine,B.
  TITLE     GLIPR2 is a negative regulator of autophagy and the
            BECN1-ATG14-containing phosphatidylinositol 3-kinase complex
  JOURNAL   Autophagy 17 (10), 2891-2904 (2021)
   PUBMED   33222586
REFERENCE   3  (bases 1 to 3942)
  AUTHORS   Ivankovic,D., Drew,J., Lesept,F., White,I.J., Lopez Domenech,G.,
            Tooze,S.A. and Kittler,J.T.
  TITLE     Axonal autophagosome maturation defect through failure of ATG9A
            sorting underpins pathology in AP-4 deficiency syndrome
  JOURNAL   Autophagy 16 (3), 391-407 (2020)
   PUBMED   31142229
REFERENCE   4  (bases 1 to 3942)
  AUTHORS   Stavoe,A.K.H. and Holzbaur,E.L.F.
  TITLE     Neuronal autophagy declines substantially with age and is rescued
            by overexpression of WIPI2
  JOURNAL   Autophagy 16 (2), 371-372 (2020)
   PUBMED   31794336
  REMARK    GeneRIF: Neuronal autophagy declines substantially with age and is
            rescued by overexpression of WIPI2.
REFERENCE   5  (bases 1 to 3942)
  AUTHORS   Stavoe,A.K., Gopal,P.P., Gubas,A., Tooze,S.A. and Holzbaur,E.L.
  TITLE     Expression of WIPI2B counteracts age-related decline in
            autophagosome biogenesis in neurons
  JOURNAL   Elife 8, e44219 (2019)
   PUBMED   31309927
  REMARK    GeneRIF: Importantly, ectopically expressing WIPI2B effectively
            restored autophagosome biogenesis in aged neurons.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3942)
  AUTHORS   Bansal,M., Moharir,S.C., Sailasree,S.P., Sirohi,K., Sudhakar,C.,
            Sarathi,D.P., Lakshmi,B.J., Buono,M., Kumar,S. and Swarup,G.
  TITLE     Optineurin promotes autophagosome formation by recruiting the
            autophagy-related Atg12-5-16L1 complex to phagophores containing
            the Wipi2 protein
  JOURNAL   J Biol Chem 293 (1), 132-147 (2018)
   PUBMED   29133525
REFERENCE   7  (bases 1 to 3942)
  AUTHORS   Zhao,Y.G., Chen,Y., Miao,G., Zhao,H., Qu,W., Li,D., Wang,Z.,
            Liu,N., Li,L., Chen,S., Liu,P., Feng,D. and Zhang,H.
  TITLE     The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA
            Activity to Control ER-Isolation Membrane Contacts for
            Autophagosome Formation
  JOURNAL   Mol Cell 67 (6), 974-989 (2017)
   PUBMED   28890335
REFERENCE   8  (bases 1 to 3942)
  AUTHORS   Akinduro,O., Sully,K., Patel,A., Robinson,D.J., Chikh,A.,
            McPhail,G., Braun,K.M., Philpott,M.P., Harwood,C.A., Byrne,C.,
            O'Shaughnessy,R.F.L. and Bergamaschi,D.
  TITLE     Constitutive Autophagy and Nucleophagy during Epidermal
            Differentiation
  JOURNAL   J Invest Dermatol 136 (7), 1460-1470 (2016)
   PUBMED   27021405
REFERENCE   9  (bases 1 to 3942)
  AUTHORS   Dooley,H.C., Razi,M., Polson,H.E., Girardin,S.E., Wilson,M.I. and
            Tooze,S.A.
  TITLE     WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and
            pathogen clearance by recruiting Atg12-5-16L1
  JOURNAL   Mol Cell 55 (2), 238-252 (2014)
   PUBMED   24954904
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158310.3 and AC105987.21.
            
            On Mar 10, 2023 this sequence version replaced NM_178398.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC044894.2, AK172030.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-278               AC158310.3         205736-206013
            279-361             AC158310.3         214383-214465
            362-531             AC105987.21        32474-32643         c
            532-628             AC105987.21        30203-30299         c
            629-726             AC105987.21        29329-29426         c
            727-819             AC105987.21        28866-28958         c
            820-890             AC105987.21        27396-27466         c
            891-998             AC105987.21        25349-25456         c
            999-1163            AC105987.21        24002-24166         c
            1164-1271           AC105987.21        22961-23068         c
            1272-1429           AC105987.21        22240-22397         c
            1430-3942           AC105987.21        19119-21631         c
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 81.53 cM"
     gene            1..3942
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /note="WD repeat domain, phosphoinositide interacting 2"
                     /db_xref="GeneID:74781"
                     /db_xref="MGI:MGI:1923831"
     exon            1..278
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1542
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat domain phosphoinositide-interacting protein 2;
                     WIPI-2"
                     /codon_start=1
                     /product="WD repeat domain phosphoinositide-interacting
                     protein 2 isoform 1"
                     /protein_id="NP_848485.1"
                     /db_xref="CCDS:CCDS19828.1"
                     /db_xref="GeneID:74781"
                     /db_xref="MGI:MGI:1923831"
                     /translation="MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVD
                     KLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTIL
                     AVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGS
                     ASIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQ
                     KLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLEAVREKPPEEPTTWTGY
                     FGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLTTIQKIPRLLVGASDG
                     YLYMYNLDPQEGGECALMRQHRLDGSMETTSEIVDSASHDCPLATQTYGTAAAKGAYV
                     PSSPTRLGKGQDANLEAYTDDLGAVGGACLEDEASALRLDEDSEHPPMILRTD"
     exon            279..361
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            362..531
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            532..628
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            629..726
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            727..819
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            820..890
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            891..998
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1163
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1271
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1429
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..3942
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1695..1700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /note="hexamer: AATACA"
     polyA_site      1722
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /note="major polyA site"
     regulatory      2085..2090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2106
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
     polyA_site      3942
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
ORIGIN      
        1 gcagcccgtc ccggctctgg agcataaaca agagtgggga cgggatgagg cggcagttgg
       61 tccccgcgca gcgagaggcg gcgagcgggg cgacctgccg gaccggacgt cgatcctgag
      121 tgtagcgtgc tcccgcgggc cgcccgagtc gagctgcgcg cgctcccgcc aaggggggtc
      181 cgaggccgtc gcgcgctcgc agccatgaac ctggcgagcc agagcggaga ggccggcgcc
      241 ggccagctgc tgttcgccaa cttcaaccag gataacacgt ccctagctgt tggtagtaag
      301 tccgggtata agtttttctc cctttcttct gtggataagc tggaacagat ctatgaatgc
      361 actgacactg aagatgtctg cattgtggag agattgttct caagcagctt ggtggccatt
      421 gtcagcctca aagctcccag gaagctgaag gtttgccatt ttaagaaggg aactgagata
      481 tgcaactaca gctactccaa cactatcctg gctgtgaagc tgaacaggca gcggctcatt
      541 gtgtgtctgg aagagtcgct ctacatacac aacatccggg acatgaaggt acttcatacc
      601 atccgagaga cacccccaaa ccctgcaggc ttgtgtgcac tgtcaataaa caatgacaac
      661 tgctacttgg cgtatccagg gagtgcgagc attggagagg tgcaggtctt cgacaccatt
      721 aacttgagag ctgcaaacat gatcccagct catgatagtc ccttagcagc cctggctttt
      781 gatgcaagtg ggaccaagct tgccactgct tctgagaagg ggactgtgat tcgggtgttt
      841 tccattccag agggacagaa gctgtttgag ttcaggagag gagtgaagag gtgtgtgagc
      901 atctgctccc tggccttcag catggacggc atgttcctct ccgcatccag caacaccgaa
      961 acagtgcaca tcttcaaact tgaggctgtg agggaaaaac ctccggaaga gcccaccacc
     1021 tggactggct actttgggaa ggttctcatg gcatctacca gctacctgcc ctcacaagtg
     1081 acagaaatgt tcaaccaggg cagggccttc gccactgtcc gcctgccatt ctgtggccac
     1141 aaaaacatct gctcactaac cacaattcag aagatcccac ggttgctggt aggagcatca
     1201 gatggctatt tgtacatgta caacctggac ccccaggagg gaggcgagtg tgcgctgatg
     1261 cgtcagcaca ggcttgatgg cagtatggag acgaccagtg aaatcgtaga ctctgcatct
     1321 catgactgcc ccttagcaac acagacgtac ggcacagcag ctgccaaagg tgcctatgtg
     1381 ccctcgtccc ccacaagact tgggaaaggg caggacgcca acttagaagc ctacacagat
     1441 gacctgggtg ctgtgggtgg tgcatgccta gaggatgaag ccagcgctct gcgcctggat
     1501 gaagacagcg aacatcctcc catgattctc cggactgact gatcctgacc tgtgaactct
     1561 ggcccaggag cagacaacac tgcctggctg aggacttgag cattgctgct atgaactttg
     1621 acctgaattg agggagagga tggcagagac ttaaagcaga ggttgtagtt atagcctatc
     1681 catactgttg agaaaataca gttttcactt cagtgacttt aatcctgctt atgaatttag
     1741 ctttttattt tctctcactc tttaattttt ttaatctttt tttttctttt ttctttttct
     1801 tttttgccaa aattaactat ttggtgaagt ctgtggagtc tccttgcttt gcatgtgtga
     1861 tgtgccaagc cagcatagga gagcaagtgg agtggccact ccatagcaaa ccacagttgt
     1921 caggtctcat gccagggcac gcatgaagaa gtctgcgtgc agtgattctg cagggacagg
     1981 gcccatgtgc ccaccaagac cagccagggc accagtggac agtggcctct gtcaggagtc
     2041 ctcttgccct actgccccac ctgggccatt ttccctccca atttattaaa ctctttccag
     2101 attggatcat tctgggacta gcttcctcca cggggggact gttgttctgg gtcttagtct
     2161 ccatgggcat cttgagaggg agaggggcag tttctgctct ctgtgccttc tcttctgaca
     2221 ggtgaacttg gcagtctacc agggcagcct ggaaccaaga aaggtgatgt cgtcagtgtc
     2281 gttaaatgct ctgtctcctc ctgtccccat ccataactgt gacttggctt tttgtttttt
     2341 ttgtttttaa aggagagtct atagctactc caattcccaa aaaagcacaa aggtgtaaga
     2401 tgctaggtca gggtcagcct ctgcggacct ggagcagtgg tgtctgccag aggctctcct
     2461 ccctccccag acatagcaga gagtagaggg gcccctcccc ctcccaggga ggcggcagga
     2521 gattccctcc cggggtgatg tgccttttct tttggtctta caagggcagg aaagtgaggt
     2581 cagatgtcag tgctgccctg tgcctgtctg cttttgattg tttgctattt ggggggaagg
     2641 tgtggtctca cactacaccc caggccctgg aactcactat atagcccagg ctggtctcag
     2701 actcaaagta gtccccctgc ctcaacttcc tgtgagttgg aattacaggt actgctgtgc
     2761 taggctccat tctcagctct atagaataaa aagttgaggt cctcaaatct gcattctaaa
     2821 gaacagagcc taacagtgga gcccaagggc gtggctgtgg cttctgcatg actatggctt
     2881 taggtgcagg ggttttggag taggaaatct ggataatgtt ggggtaccag gttaatccag
     2941 ccggataaga gggactacag gtagctgcct tcaggcctgt gtggttatgt ctggtagagt
     3001 atatctgcag ggtgcctgta gctaaatctg ctgaggttcc ctgtttctgc tgttcaagga
     3061 tagctaatca cataattggg gtttgagggg cgcccactgt tagctctggc tgctgttggc
     3121 acctgtgccc tggagcatcc gaggagccaa acagtatgag gcagggttag gctgcgtgtg
     3181 ctggacttta aactatgaat tttctggacc ctaataacat gggaccccag cctgtggcct
     3241 agttcagtga ctgcttcact gggagactaa aactggcctt cacaggaacc gactgtggga
     3301 agcacacggg gctgattggg agccggggtt tcctggaacc aaagagtgtg gctgttgtgc
     3361 tggtgacctt ggctttgtgc tctgggtttg gagctcagct ccctctgctg aggcctctct
     3421 agggagctgg ggtctcacag acagtggagt tcacgtctcc ctaaagcata ggtttcctca
     3481 cttggaaact tggttagaaa aaggccgctc ctggggtctg gggctgtgcc ttccatcagt
     3541 ggtcttgcca ggctgtgcaa atttggtgag ccttcctctc ttgggacaga tgtgcccgtg
     3601 catcccacag aggtcagaaa agcaaaggat taggctgtga cctcaggcaa tctgtacagc
     3661 actcgcaggc ctcaggtgag ggaggagaga gtggctggct gaggtcagca ctcactgttg
     3721 ggatgcacac ggtagagcca agcctcagca gggctcaggt tcaaaggtaa gaacattttg
     3781 gaatccggtg tggcacacac ctgtaatccc agcagtcaag agagtgaggc aggagcatgg
     3841 ctgtaaattt gaggccagtc tagactatat actcctgggc aaacctggcc tacagagtga
     3901 gaccctgtct caaaaaacca aaggatgatg actttaagaa aa
//
