LOCUS       NP_848485                445 aa            linear   ROD 02-MAY-2024
DEFINITION  WD repeat domain phosphoinositide-interacting protein 2 isoform 1
            [Mus musculus].
ACCESSION   NP_848485
VERSION     NP_848485.1
DBSOURCE    REFSEQ: accession NM_178398.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 445)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 445)
  AUTHORS   Zhao,Y., Zou,Z., Sun,D., Li,Y., Sinha,S.C., Yu,L., Bennett,L. and
            Levine,B.
  TITLE     GLIPR2 is a negative regulator of autophagy and the
            BECN1-ATG14-containing phosphatidylinositol 3-kinase complex
  JOURNAL   Autophagy 17 (10), 2891-2904 (2021)
   PUBMED   33222586
REFERENCE   3  (residues 1 to 445)
  AUTHORS   Ivankovic,D., Drew,J., Lesept,F., White,I.J., Lopez Domenech,G.,
            Tooze,S.A. and Kittler,J.T.
  TITLE     Axonal autophagosome maturation defect through failure of ATG9A
            sorting underpins pathology in AP-4 deficiency syndrome
  JOURNAL   Autophagy 16 (3), 391-407 (2020)
   PUBMED   31142229
REFERENCE   4  (residues 1 to 445)
  AUTHORS   Stavoe,A.K.H. and Holzbaur,E.L.F.
  TITLE     Neuronal autophagy declines substantially with age and is rescued
            by overexpression of WIPI2
  JOURNAL   Autophagy 16 (2), 371-372 (2020)
   PUBMED   31794336
  REMARK    GeneRIF: Neuronal autophagy declines substantially with age and is
            rescued by overexpression of WIPI2.
REFERENCE   5  (residues 1 to 445)
  AUTHORS   Stavoe,A.K., Gopal,P.P., Gubas,A., Tooze,S.A. and Holzbaur,E.L.
  TITLE     Expression of WIPI2B counteracts age-related decline in
            autophagosome biogenesis in neurons
  JOURNAL   Elife 8, e44219 (2019)
   PUBMED   31309927
  REMARK    GeneRIF: Importantly, ectopically expressing WIPI2B effectively
            restored autophagosome biogenesis in aged neurons.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 445)
  AUTHORS   Bansal,M., Moharir,S.C., Sailasree,S.P., Sirohi,K., Sudhakar,C.,
            Sarathi,D.P., Lakshmi,B.J., Buono,M., Kumar,S. and Swarup,G.
  TITLE     Optineurin promotes autophagosome formation by recruiting the
            autophagy-related Atg12-5-16L1 complex to phagophores containing
            the Wipi2 protein
  JOURNAL   J Biol Chem 293 (1), 132-147 (2018)
   PUBMED   29133525
REFERENCE   7  (residues 1 to 445)
  AUTHORS   Zhao,Y.G., Chen,Y., Miao,G., Zhao,H., Qu,W., Li,D., Wang,Z.,
            Liu,N., Li,L., Chen,S., Liu,P., Feng,D. and Zhang,H.
  TITLE     The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA
            Activity to Control ER-Isolation Membrane Contacts for
            Autophagosome Formation
  JOURNAL   Mol Cell 67 (6), 974-989 (2017)
   PUBMED   28890335
REFERENCE   8  (residues 1 to 445)
  AUTHORS   Akinduro,O., Sully,K., Patel,A., Robinson,D.J., Chikh,A.,
            McPhail,G., Braun,K.M., Philpott,M.P., Harwood,C.A., Byrne,C.,
            O'Shaughnessy,R.F.L. and Bergamaschi,D.
  TITLE     Constitutive Autophagy and Nucleophagy during Epidermal
            Differentiation
  JOURNAL   J Invest Dermatol 136 (7), 1460-1470 (2016)
   PUBMED   27021405
REFERENCE   9  (residues 1 to 445)
  AUTHORS   Dooley,H.C., Razi,M., Polson,H.E., Girardin,S.E., Wilson,M.I. and
            Tooze,S.A.
  TITLE     WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and
            pathogen clearance by recruiting Atg12-5-16L1
  JOURNAL   Mol Cell 55 (2), 238-252 (2014)
   PUBMED   24954904
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158310.3 and AC105987.21.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC044894.2, AK172030.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 81.53 cM"
     Protein         1..445
                     /product="WD repeat domain phosphoinositide-interacting
                     protein 2 isoform 1"
                     /note="WD repeat domain phosphoinositide-interacting
                     protein 2; WIPI-2"
                     /calculated_mol_wt=48346
     Region          18..53
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          58..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          102..134
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <118..342
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          143..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..222
                     /region_name="WD 1. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80W47.1)"
     Region          188..228
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          223..226
                     /region_name="L/FRRG motif.
                     /evidence=ECO:0000303|PubMed:24954904"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80W47.1)"
     Region          228..267
                     /region_name="WD 2. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80W47.1)"
     Region          233..266
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          274..309
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..349
                     /region_name="WD 3. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80W47.1)"
     Site            395
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y4P8; propagated from
                     UniProtKB/Swiss-Prot (Q80W47.1)"
     CDS             1..445
                     /gene="Wipi2"
                     /gene_synonym="1110018O08Rik; 2510001I10Rik"
                     /coded_by="NM_178398.5:205..1542"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS19828.1"
                     /db_xref="GeneID:74781"
                     /db_xref="MGI:MGI:1923831"
ORIGIN      
        1 mnlasqsgea gagqllfanf nqdntslavg sksgykffsl ssvdkleqiy ectdtedvci
       61 verlfssslv aivslkaprk lkvchfkkgt eicnysysnt ilavklnrqr livcleesly
      121 ihnirdmkvl htiretppnp aglcalsinn dncylaypgs asigevqvfd tinlraanmi
      181 pahdsplaal afdasgtkla tasekgtvir vfsipegqkl fefrrgvkrc vsicslafsm
      241 dgmflsassn tetvhifkle avrekppeep ttwtgyfgkv lmastsylps qvtemfnqgr
      301 afatvrlpfc ghknicsltt iqkiprllvg asdgylymyn ldpqeggeca lmrqhrldgs
      361 mettseivds ashdcplatq tygtaaakga yvpssptrlg kgqdanleay tddlgavgga
      421 cledeasalr ldedsehppm ilrtd
//
