LOCUS       NM_181819               3392 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus WAP, follistatin/kazal, immunoglobulin, kunitz and
            netrin domain containing 2 (Wfikkn2), mRNA.
ACCESSION   NM_181819
VERSION     NM_181819.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Parente,A., Boukredine,A., Baraige,F., Duprat,N.,
            Gondran-Tellier,V., Magnol,L. and Blanquet,V.
  TITLE     GASP-2 overexpressing mice exhibit a hypermuscular phenotype with
            contrasting molecular effects compared to GASP-1 transgenics
  JOURNAL   FASEB J 34 (3), 4026-4040 (2020)
   PUBMED   31960486
REFERENCE   3  (bases 1 to 3392)
  AUTHORS   McCoy,J.C., Walker,R.G., Murray,N.H. and Thompson,T.B.
  TITLE     Crystal structure of the WFIKKN2 follistatin domain reveals insight
            into how it inhibits growth differentiation factor 8 (GDF8) and
            GDF11
  JOURNAL   J Biol Chem 294 (16), 6333-6343 (2019)
   PUBMED   30814254
  REMARK    GeneRIF: results indicate that both WFIKKN and follistatin utilize
            their FSDs to block the type II receptor but do so via different
            binding interactions.
REFERENCE   4  (bases 1 to 3392)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3392)
  AUTHORS   Lee,Y.S. and Lee,S.J.
  TITLE     Regulation of GDF-11 and myostatin activity by GASP-1 and GASP-2
  JOURNAL   Proc Natl Acad Sci U S A 110 (39), E3713-E3722 (2013)
   PUBMED   24019467
REFERENCE   6  (bases 1 to 3392)
  AUTHORS   Martini,L., Thompson,D., Kharazia,V. and Whistler,J.L.
  TITLE     Differential regulation of behavioral tolerance to WIN55,212-2 by
            GASP1
  JOURNAL   Neuropsychopharmacology 35 (6), 1363-1373 (2010)
   PUBMED   20164830
  REMARK    GeneRIF: Taken together, this study provides evidence that GASP1
            regulates CB1 receptor downregulation in vivo, and that
            postendocytic receptor trafficking has a key role in the
            development of tolerance to WIN55,212-2.
REFERENCE   7  (bases 1 to 3392)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
REFERENCE   8  (bases 1 to 3392)
  AUTHORS   Kondas,K., Szlama,G., Trexler,M. and Patthy,L.
  TITLE     Both WFIKKN1 and WFIKKN2 have high affinity for growth and
            differentiation factors 8 and 11
  JOURNAL   J Biol Chem 283 (35), 23677-23684 (2008)
   PUBMED   18596030
REFERENCE   9  (bases 1 to 3392)
  AUTHORS   Hill,J.J., Qiu,Y., Hewick,R.M. and Wolfman,N.M.
  TITLE     Regulation of myostatin in vivo by growth and differentiation
            factor-associated serum protein-1: a novel protein with protease
            inhibitor and follistatin domains
  JOURNAL   Mol Endocrinol 17 (6), 1144-1154 (2003)
   PUBMED   12595574
  REMARK    GeneRIF: Report of the identification of growth and differentiation
            factor-associated serum protein-1 (GASP-1) associated with
            endogenous myostatin in normal serum
REFERENCE   10 (bases 1 to 3392)
  AUTHORS   Moffatt,P., Salois,P., Gaumond,M.H., St-Amant,N., Godin,E. and
            Lanctot,C.
  TITLE     Engineered viruses to select genes encoding secreted and
            membrane-bound proteins in mammalian cells
  JOURNAL   Nucleic Acids Res 30 (19), 4285-4294 (2002)
   PUBMED   12364608
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645846.12.
            
            On Nov 13, 2009 this sequence version replaced NM_181819.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY308804.1, JQ080910.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               AL645846.12        111675-111899       c
            226-3392            AL645846.12        105272-108438       c
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 58.9 cM"
     gene            1..3392
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="WAP, follistatin/kazal, immunoglobulin, kunitz and
                     netrin domain containing 2"
                     /db_xref="GeneID:278507"
                     /db_xref="MGI:MGI:2669209"
     exon            1..225
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1746
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="WAP, kazal, immunoglobulin, kunitz and NTR
                     domain-containing protein 2; growth and differentiation
                     factor-associated serum protein 1; GASP-1; mGASP-1"
                     /codon_start=1
                     /product="WAP, Kazal, immunoglobulin, Kunitz and NTR
                     domain-containing protein 2 precursor"
                     /protein_id="NP_861540.2"
                     /db_xref="CCDS:CCDS25251.1"
                     /db_xref="GeneID:278507"
                     /db_xref="MGI:MGI:2669209"
                     /translation="MCAPGYHRFWFHWGLLLLLLLEAPLRGLALPPIRYSHAGICPND
                     MNPNLWVDAQSTCKRECETDQECETYEKCCPNVCGTKSCVAARYMDVKGKKGPVGMPK
                     EATCDHFMCLQQGSECDIWDGQPVCKCKDRCEKEPSFTCASDGLTYYNRCFMDAEACS
                     KGITLSVVTCRYHFTWPNTSPPPPETTVHPTTASPETLGLDMAAPALLNHPVHQSVTV
                     GETVSFLCDVVGRPRPELTWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYNVQPQD
                     AGIYTCTARNVAGVLRADFPLSVVRGGQARATSESSLNGTAFPATECLKPPDSEDCGE
                     EQTRWHFDAQANNCLTFTFGHCHHNLNHFETYEACMLACMSGPLAICSLPALQGPCKA
                     YVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESCPFPRGNQHCRACKPRQKLV
                     TSFCRSDFVILGRVSELTEEQDSGRALVTVDEVLKDEKMGLKFLGREPLEVTLLHVDW
                     TCPCPNVTVGETPLIIMGEVDGGMAMLRPDSFVGASSTRRVRKLREVMYKKTCDVLKD
                     FLGLQ"
     exon            226..3392
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3364..3369
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="hexamer: AATAAA"
     regulatory      3368..3373
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="hexamer: AATAAA"
     polyA_site      3385
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="major polyA site"
     polyA_site      3392
                     /gene="Wfikkn2"
                     /gene_synonym="2610304F08Rik; Gasp1; WFIKKNRP"
                     /note="major polyA site"
ORIGIN      
        1 gggtaccctc tgaccactgc agacggcacc atgtgtgccc cagggtatca tcggttctgg
       61 tttcactggg ggctgctgtt gctgctgctc ctcgaggctc cccttcgagg cctagcactg
      121 ccacccatcc gatactccca tgcgggcatc tgccccaacg acatgaaccc caacctctgg
      181 gtggatgccc agagcacctg caagcgagag tgtgaaacag accaggaatg tgagacctat
      241 gagaaatgct gccccaatgt gtgtgggacc aagagctgtg tggcagcccg ctacatggat
      301 gtgaaaggga agaagggccc tgtgggcatg cccaaggagg ccacatgtga ccatttcatg
      361 tgcctgcagc agggctctga gtgtgacatc tgggacggcc agcccgtgtg taagtgcaaa
      421 gatcgctgtg agaaggagcc cagcttcacc tgtgcctctg atggccttac ctactacaac
      481 cgttgcttca tggacgccga agcctgctcc aagggcatca cactgtctgt ggtcacctgt
      541 cgttatcact tcacctggcc taacaccagc cctccaccgc ctgagaccac ggtgcatccc
      601 accaccgcct ctccggagac tctcgggctg gacatggcag ccccggccct gctcaaccat
      661 cctgtccatc agtcagtcac cgtgggtgag actgtgagtt tcctctgtga cgtggtaggc
      721 cggcctcggc cagagctcac ttgggagaaa cagctggagg accgagagaa tgttgtcatg
      781 aggcccaacc acgtgcgcgg taatgtggtg gtcactaaca ttgcccagct ggtcatctac
      841 aacgtccagc cccaggatgc tggcatatac acctgtacag ctcgaaatgt cgctggtgtc
      901 ctgagggctg acttcccgtt gtcggtggtc aggggtggtc aggccagggc cacttcagag
      961 agcagtctca atggcacagc ttttccagca acagagtgcc tgaagccccc agacagtgag
     1021 gactgtggag aggagcagac acgctggcac ttcgacgccc aggctaacaa ctgcctcact
     1081 ttcacctttg gtcactgcca ccacaatctc aaccactttg agacctacga ggcctgtatg
     1141 ctggcttgta tgagtgggcc attggccatc tgcagcctgc ctgccctgca agggccttgc
     1201 aaagcttatg tcccacgctg ggcctacaac agccagacag gcctatgcca gtccttcgtc
     1261 tatggcggct gtgagggcaa cggtaacaac tttgaaagcc gtgaggcttg tgaggagtcg
     1321 tgtcccttcc cgaggggtaa ccagcactgc cgggcctgca agccccggca aaaacttgtt
     1381 accagcttct gtcggagtga ctttgtcatc ctgggcaggg tctctgagct gaccgaagag
     1441 caagactcag gccgtgccct ggtgaccgtg gatgaggtct taaaagatga gaagatgggc
     1501 ctcaagtttc tgggccggga gcctctggaa gtcaccctgc ttcatgtaga ctggacctgt
     1561 ccttgcccca acgtgacagt gggtgagaca ccactcatca tcatggggga ggtggacggc
     1621 ggcatggcca tgctgagacc cgatagcttt gtgggggcat cgagcacacg gcgggtcagg
     1681 aagctccgtg aggtcatgta caagaaaacc tgtgacgtcc tcaaggactt cctgggcttg
     1741 caatgaagct agctcgtcta ccctgaccct cctccaccca cccacccacc cacccctgcc
     1801 ctcagtcagc caccaggtga cttgaggtta gacagctttg gctaggaaag aagcattagc
     1861 taatatgtgg ggagaaagcc acccgtaaag cctccgaagt tttagcacca tctctatgtt
     1921 ctctctgcct gtccgtggtc tcactttgaa aatggtagtg agcactagcc ccctttagag
     1981 gtctgtggcc catcgcttcc catactacca gcgctgtgac cttggacaag ttgcttaacc
     2041 tctctgtgcc ttagattatt gtgaacaaag tgggaagagt ggttgtaccc cctgggctta
     2101 gaagaaagca gtgagctaaa ggaagtcggg gaacctgaca ggttctttgg gttccataaa
     2161 atgtctgtat ctctgcccca gcccccagcc tccaccccag acaaggtttc tctgtgtagc
     2221 cctggctggc ctggaattca ctctgtagac caggttggcc atgaactcag agatctgcct
     2281 gcctctgcct cccaagtgct gggattaaaa gcgtgcacca ccacgtctgg ctagaaggtc
     2341 tgtatctctg atagctgaga agggggctgg cagggggaag aaagggcata ggtaagttgt
     2401 ttcccatggg tggttccctc gggcagtctc gtcccccttt gctgacccat gcccctctgc
     2461 ccagtcgcct agcctggggt caggaggaga tgccatctgt attgtagaat ggtttagctt
     2521 gtataggagg tatgtgggaa gagagagata agagagttcg agggtcattt aagacagctg
     2581 ccctactgtg tgccgcccca ctacacggct gtagtcagag accatccaca tcaagggtcg
     2641 tgaacatcaa gaagccaaag cttcaaccta ggctctaact tctcctaatg gagactgaag
     2701 ggccggctgc ctttggtgtc accacaccaa gcgaatccat gttttctttc cttagctgcc
     2761 ttggtttgct tgtgtgcttc tgtacatttc agctcagtga acacttcctg ggtgccaggt
     2821 gctaggacca tagagactga ttctgctcta ctggccgagt ctgaaggccg tcctgtatct
     2881 cagctccccc aggcgccagt gggtgattga cagcggctag gcattttcat ctgatgagcc
     2941 aggtgctttc atgtgctgtg gacttcctag gaaaagccca aagatctgtt ttggtagcaa
     3001 gagaaataaa gaagacaaag gcaggaggat cagggggcag cctaggcacc agagaggatg
     3061 tgggcccagg gagaatgaac attgcttgat gctgccatct agtggtgcat atgggcacct
     3121 tcagggatca ggaggaaaga tctatctggg tggggcctgg gaggggcctc ttggcgatgg
     3181 cagctctgag atcacttgtt tcttctggtc ccagatacaa aggcactgtg tagaggattt
     3241 ggaacccctc tgtcatctct gcatctgccc tgttccctcc tcagaccttc attctttcga
     3301 catccctcca aaggaagcac tgtgggatgc cctaatggct tcctcaaata tacggaggac
     3361 gataataaat aaaatttcat agtgtcaact gc
//
