LOCUS       NM_183249                452 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus WAP four-disulfide core domain 21 (Wfdc21), mRNA.
ACCESSION   NM_183249
VERSION     NM_183249.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Xie,Z., Guo,Z. and Liu,J.
  TITLE     Whey Acidic Protein/Four-Disulfide Core Domain 21 Regulate Sepsis
            Pathogenesis in a Mouse Model and a Macrophage Cell Line via the
            Stat3/Toll-Like Receptor 4 (TLR4) Signaling Pathway
  JOURNAL   Med Sci Monit 24, 4054-4063 (2018)
   PUBMED   29900929
  REMARK    GeneRIF: Authors found that Wfdc21 level was elevated in the
            CLP-induced animal model and LPS-treated RAW264.7 cells.
            Furthermore, the downregulation of Wfdc21 modulated the
            concentration of pro-inflammatory factors in LPS-treated
            macrophages, such as IL-1beta and TNF-alpha, in LPS-treated
            macrophages.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  AUTHORS   Wu,Y. and Smas,C.M.
  TITLE     Wdnm1-like, a new adipokine with a role in MMP-2 activation
  JOURNAL   Am J Physiol Endocrinol Metab 295 (1), E205-E215 (2008)
   PUBMED   18492766
REFERENCE   3  (bases 1 to 452)
  AUTHORS   Adachi,W., Ulanovsky,H., Li,Y., Norman,B., Davis,J. and
            Piatigorsky,J.
  TITLE     Serial analysis of gene expression (SAGE) in the rat limbal and
            central corneal epithelium
  JOURNAL   Invest Ophthalmol Vis Sci 47 (9), 3801-3810 (2006)
   PUBMED   16936091
  REMARK    GeneRIF: PMID:16936091 mostly concerns studies in rat, but shows
            similarity between rat and mouse WDNM1-like proteins.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY082582.1, AA254806.1 and BY094209.1.
            
            On Mar 1, 2007 this sequence version replaced NM_183249.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY094209.1, BY449514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-353               BY082582.1         1-353
            354-409             AA254806.1         284-339
            410-452             BY094209.1         353-395
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.23 cM"
     gene            1..452
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /note="WAP four-disulfide core domain 21"
                     /db_xref="GeneID:66107"
                     /db_xref="MGI:MGI:1913357"
     exon            1..184
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..279
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /note="protein Wfdc21"
                     /codon_start=1
                     /product="protein Wfdc21 precursor"
                     /protein_id="NP_899072.1"
                     /db_xref="CCDS:CCDS36258.1"
                     /db_xref="GeneID:66107"
                     /db_xref="MGI:MGI:1913357"
                     /translation="MKLGAFLLLVSLITLSLEVQELQAAVRPLQLLGTCAELCRGDWD
                     CGPEEQCVSIGCSHICTTN"
     exon            185..285
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /inference="alignment:Splign:2.1.0"
     exon            286..438
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /inference="alignment:Splign:2.1.0"
     regulatory      410..415
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /note="hexamer: AATAAA"
     polyA_site      436
                     /gene="Wfdc21"
                     /gene_synonym="1100001G20Rik; Wdnm1-like"
                     /note="major polyA site"
ORIGIN      
        1 ttgctgccta gctcagtggg tgaagaggtt taaattctgg cagctcttgg ccctgctgcg
       61 tattggctaa catccttcag cagcagcatg aagctaggag ccttccttct gttggtgtcc
      121 ctcatcaccc tcagcctaga ggtacaggag ctgcaggctg cagtgagacc tctgcagctt
      181 ttaggcacct gtgctgagct ctgccgtggt gactgggact gtgggccaga ggaacaatgt
      241 gtcagtattg gatgcagtca catctgtact acaaactaaa aacagcttct acctggaaaa
      301 aaaaatgtgt ctgtttggag ctctgtgacc aagaaaacag ttgaaaatgg aggccatgta
      361 tggagattac aagcagcaca gtggagtggg acaaggagtt gtttctttta ataaatcatt
      421 aatgtaaaag tctcaacaaa aaaaaaaaaa aa
//
