LOCUS       NM_001379396            2017 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus WD repeat, SAM and U-box domain containing 1 (Wdsub1),
            transcript variant 4, mRNA.
ACCESSION   NM_001379396 XM_030252106
VERSION     NM_001379396.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Guan,Y., Du,H.B., Yang,Z., Wang,Y.Z., Ren,R., Liu,W.W., Zhang,C.,
            Zhang,J.H., An,W.T., Li,N.N., Zeng,X.X., Li,J., Sun,Y.X.,
            Wang,Y.F., Yang,F., Yang,J., Xiong,W., Yu,X., Chai,R.J., Tu,X.M.,
            Sun,J.P. and Xu,Z.G.
  TITLE     Deafness-Associated ADGRV1 Mutation Impairs USH2A Stability through
            Improper Phosphorylation of WHRN and WDSUB1 Recruitment
  JOURNAL   Adv Sci (Weinh) 10 (16), e2205993 (2023)
   PUBMED   37066759
  REMARK    GeneRIF: Deafness-Associated ADGRV1 Mutation Impairs USH2A
            Stability through Improper Phosphorylation of WHRN and WDSUB1
            Recruitment.
REFERENCE   3  (bases 1 to 2017)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929242.16.
            
            On Apr 1, 2020 this sequence version replaced XM_030252106.1.
            
            Transcript Variant: This variant (4), as well as variants 1, 3, and
            5, encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1617437.1,
                                           ERR3363658.733498.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               AL929242.16        35929-36057         c
            130-550             AL929242.16        31581-32001         c
            551-735             AL929242.16        30118-30302         c
            736-825             AL929242.16        27690-27779         c
            826-919             AL929242.16        26667-26760         c
            920-953             AL929242.16        17753-17786         c
            954-994             AL929242.16        16268-16308         c
            995-1101            AL929242.16        16071-16177         c
            1102-1278           AL929242.16        15012-15188         c
            1279-1419           AL929242.16        12061-12201         c
            1420-2017           AL929242.16        5815-6412           c
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 34.14 cM"
     gene            1..2017
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /note="WD repeat, SAM and U-box domain containing 1"
                     /db_xref="GeneID:72137"
                     /db_xref="MGI:MGI:1919387"
     exon            1..129
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            130..550
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1577
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /note="isoform 1 is encoded by transcript variant 4; WD
                     repeat, SAM and U-box domain-containing protein 1"
                     /codon_start=1
                     /product="WD repeat, SAM and U-box domain-containing
                     protein 1 isoform 1"
                     /protein_id="NP_001366325.1"
                     /db_xref="GeneID:72137"
                     /db_xref="MGI:MGI:1919387"
                     /translation="MVRLIHTLADHGDDVSCCAFSAALLATCSLDKTIRLYSLSDFAE
                     LPYSPLKFHTYAVHCCCFSPSGHVLASCSTDGTTVLWSSHSGHTLTVLEQPGGSPVRV
                     CCFSPDSAYLASGAADGSIALWNAQTYKLYRCGSVKDSSLVACAFSPDGGLFVTGSSG
                     GDLTVWDDRMRCLHSEKAHDLGITCCSFSSQPLSGGEGLQSYQLASCGQDCEIKLWAV
                     TITRVLGFELKYKSTLSGHCAPVLACAFSHDGKMLASGSVDKSVIIHGIGPQSVLHTL
                     TQHTRYVTTCAFAPNTLLLATGSMDKTVNIWQFDLETPCQGSMNDPLKHFTEEWSEED
                     VSVWLRAQGLEDLVGIFRANNIDGKELLHLTKESLAGDLKIESLGLRSKVLRSIEELR
                     AKMDSLSSGIPDEFICPITRELMKDPVIASDGYSYEREAMESWIHKKKRTSPMTNLAL
                     PSLVLTPNRTLKMAINRWLETHEK"
     exon            551..735
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            736..825
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            826..919
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            920..953
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            954..994
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1101
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1278
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1419
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..2017
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1993..1998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2017
                     /gene="Wdsub1"
                     /gene_synonym="1700048E19Rik; 2610014F08Rik"
                     /note="major polyA site"
ORIGIN      
        1 agttaggtaa cacgtgaccc agtgagtcgg gctctgccta gtgagcagcc cagcccagca
       61 gcccagctcg gggcccagcc cggggccgag cccggggcag tgcagacttg gtgacctcag
      121 aacccggagg ctgtttactt tgtgtgagga acatggtgag gctgattcac acgctggctg
      181 atcacggtga tgacgtcagc tgctgcgcct tctcggctgc cctcctggcc acctgctcct
      241 tggacaagac catccgcctg tactccctaa gtgactttgc tgaactgccg tactccccgc
      301 tgaagttcca cacctatgct gtccactgct gctgtttctc accctcagga cacgttttag
      361 catcgtgctc gacagacggg accacggtgc tgtggagctc gcacagcgga cacaccctga
      421 ccgtgttgga gcagccgggt ggcagccctg tgcgcgtctg ttgcttttcc ccagactctg
      481 cctacctagc gtcaggggct gccgatggat ccattgcttt gtggaatgca cagacataca
      541 aactatatag gtgtggtagt gtcaaggata gctcattggt ggcctgtgcg ttttctcccg
      601 atggaggcct ctttgtcact ggctcctcgg gcggggactt gacagtgtgg gatgacagaa
      661 tgaggtgtct acacagcgag aaggcgcacg atctcgggat cacctgctgc agcttttcct
      721 cacagcctct ctctggcgga gaaggcctcc agtcttacca gttggcgtca tgtggtcaag
      781 actgtgaaat caaactctgg gctgttacta ttacccgtgt cttaggcttt gaattaaaat
      841 ataaaagcac actaagtggg cactgcgccc ctgttctggc ctgtgctttt tcacatgatg
      901 gaaagatgct tgcatcgggg tcagtggata aatctgtcat catacatggt atcggccctc
      961 agagtgtgct acacacgctg actcagcata ccaggtatgt tacgacttgt gcgtttgcac
     1021 ccaacactct cttacttgct actggttcaa tggacaagac agtgaacatt tggcagtttg
     1081 acctggaaac accttgccaa ggaagcatga acgacccgct gaaacatttc actgaagaat
     1141 ggtcagagga ggatgtctcc gtgtggcttc gtgctcaagg cttggaagac ctcgtcggta
     1201 ttttcagggc aaacaacatc gatgggaaag aactattgca tctcacaaag gaaagtctgg
     1261 ctggtgattt gaaaatcgaa tctctagggc tgcgcagcaa agtcctgagg agtattgaag
     1321 agctcagggc caagatggat tccctctctt ccggaatccc tgacgagttc atctgcccaa
     1381 taaccagaga actcatgaag gaccccgtca tcgcatcaga tggctactcc tacgagagag
     1441 aagcaatgga aagctggatc cacaagaaga agcgtacgag ccccatgaca aatttggctc
     1501 tcccttcact ggtactgacc ccaaacagga cactgaagat ggccatcaac cgatggctgg
     1561 agacgcacga gaagtgaacg cgttcacagg catcggatcc actttcagtg atgccctgca
     1621 aatgattcaa aatgctaagc agccatcacg aaagcaaaat aaaaggaaaa gacaaatgtt
     1681 caattcagtt acttttaaaa actgtaaatt atgagcaggg cagtggtggt gcccaccttt
     1741 aatcccagca ctcaggaggc agagacaggt ggatctccag gatcaggagt tccaggacag
     1801 cccagtttat agggcaagtc tcaggacggc caaggctaca cagagaaacc ctgtctcaaa
     1861 aaacccaaaa ccaaaaaaaa aaaaaaaaaa agtcaattat ctttaaaaca cagatttata
     1921 tatctattgt cattgctatt tctgtaaagg tgaaaatatt tttttttttg caataatgag
     1981 aaactatgta gaaataaaac ttcactatga ctttaaa
//
