LOCUS       NM_021322               4046 bp    mRNA    linear   ROD 19-NOV-2024
DEFINITION  Mus musculus WD repeat domain 4 (Wdr4), transcript variant 1, mRNA.
ACCESSION   NM_021322 XM_030249933 XM_904489
VERSION     NM_021322.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Li,Q., Jiang,S., Lei,K., Han,H., Chen,Y., Lin,W., Xiong,Q., Qi,X.,
            Gan,X., Sheng,R., Wang,Y., Zhang,Y., Ma,J., Li,T., Lin,S., Zhou,C.,
            Chen,D. and Yuan,Q.
  TITLE     Metabolic rewiring during bone development underlies tRNA
            m7G-associated primordial dwarfism
  JOURNAL   J Clin Invest 134 (20), e177220 (2024)
   PUBMED   39255038
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4046)
  AUTHORS   Wu,P.R., Chiang,S.Y., Midence,R., Kao,W.C., Lai,C.L., Cheng,I.C.,
            Chou,S.J., Chen,C.C., Huang,C.Y. and Chen,R.H.
  TITLE     Wdr4 promotes cerebellar development and locomotion through
            Arhgap17-mediated Rac1 activation
  JOURNAL   Cell Death Dis 14 (1), 52 (2023)
   PUBMED   36681682
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4046)
  AUTHORS   Lee,C.C. and Hsieh,T.S.
  TITLE     Wuho/WDR4 deficiency inhibits cell proliferation and induces
            apoptosis via DNA damage in mouse embryonic fibroblasts
  JOURNAL   Cell Signal 47, 16-26 (2018)
   PUBMED   29574139
  REMARK    GeneRIF: WDR4 protein deficiency would induces gammaH2AX protein
            level elevation, heterochromatin relaxation and DNA damage
            down-stream sequences, including p53 activation, caspase-mediated
            apoptotic pathway, and p21-mediated G2/M cell cycle arrest.
REFERENCE   5  (bases 1 to 4046)
  AUTHORS   Wang,Y.T., Chen,J., Chang,C.W., Jen,J., Huang,T.Y., Chen,C.M.,
            Shen,R., Liang,S.Y., Cheng,I.C., Yang,S.C., Lai,W.W., Cheng,K.H.,
            Hsieh,T.S., Lai,M.Z., Cheng,H.C., Wang,Y.C. and Chen,R.H.
  TITLE     Ubiquitination of tumor suppressor PML regulates prometastatic and
            immunosuppressive tumor microenvironment
  JOURNAL   J Clin Invest 127 (8), 2982-2997 (2017)
   PUBMED   28691927
REFERENCE   6  (bases 1 to 4046)
  AUTHORS   Kenney-Hunt,J.P., Vaughn,T.T., Pletscher,L.S., Peripato,A.,
            Routman,E., Cothran,K., Durand,D., Norgard,E., Perel,C. and
            Cheverud,J.M.
  TITLE     Quantitative trait loci for body size components in mice
  JOURNAL   Mamm Genome 17 (6), 526-537 (2006)
   PUBMED   16783635
REFERENCE   7  (bases 1 to 4046)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 4046)
  AUTHORS   Gitton,Y., Dahmane,N., Baik,S., Ruiz i Altaba,A., Neidhardt,L.,
            Scholze,M., Herrmann,B.G., Kahlem,P., Benkahla,A., Schrinner,S.,
            Yildirimman,R., Herwig,R., Lehrach,H. and Yaspo,M.L.
  CONSRTM   HSA21 expression map initiative
  TITLE     A gene expression map of human chromosome 21 orthologues in the
            mouse
  JOURNAL   Nature 420 (6915), 586-590 (2002)
   PUBMED   12466855
REFERENCE   9  (bases 1 to 4046)
  AUTHORS   Reymond,A., Marigo,V., Yaylaoglu,M.B., Leoni,A., Ucla,C.,
            Scamuffa,N., Caccioppoli,C., Dermitzakis,E.T., Lyle,R., Banfi,S.,
            Eichele,G., Antonarakis,S.E. and Ballabio,A.
  TITLE     Human chromosome 21 gene expression atlas in the mouse
  JOURNAL   Nature 420 (6915), 582-586 (2002)
   PUBMED   12466854
REFERENCE   10 (bases 1 to 4046)
  AUTHORS   Michaud,J., Kudoh,J., Berry,A., Bonne-Tamir,B., Lalioti,M.D.,
            Rossier,C., Shibuya,K., Kawasaki,K., Asakawa,S., Minoshima,S.,
            Shimizu,N., Antonarakis,S.E. and Scott,H.S.
  TITLE     Isolation and characterization of a human chromosome 21q22.3 gene
            (WDR4) and its mouse homologue that code for a WD-repeat protein
  JOURNAL   Genomics 68 (1), 71-79 (2000)
   PUBMED   10950928
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166172.1.
            
            On or before Apr 20, 2022 this sequence version replaced
            XM_030249933.2, NM_021322.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.525699.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC166172.1         46907-47040
            135-156             AC166172.1         48039-48060
            157-477             AC166172.1         54391-54711
            478-546             AC166172.1         56232-56300
            547-690             AC166172.1         56966-57109
            691-847             AC166172.1         63212-63368
            848-960             AC166172.1         63995-64107
            961-1021            AC166172.1         65603-65663
            1022-1120           AC166172.1         66166-66264
            1121-1185           AC166172.1         67005-67069
            1186-1369           AC166172.1         67706-67889
            1370-1439           AC166172.1         68311-68380
            1440-4046           AC166172.1         69950-72556
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 16.56 cM"
     gene            1..4046
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="WD repeat domain 4"
                     /db_xref="GeneID:57773"
                     /db_xref="MGI:MGI:1889002"
     exon            1..134
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            135..156
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            157..477
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     CDS             260..1630
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="isoform a is encoded by transcript variant 1; tRNA
                     (guanine-N(7)-)-methyltransferase subunit WDR4; WD
                     repeat-containing protein 4; WD40 repeat protein 4; tRNA
                     (guanine-N(7)-)-methyltransferase non-catalytic subunit
                     WDR4; protein Wuho homolog"
                     /codon_start=1
                     /product="tRNA (guanine-N(7)-)-methyltransferase
                     non-catalytic subunit WDR4 isoform a"
                     /protein_id="NP_067297.2"
                     /db_xref="CCDS:CCDS28606.2"
                     /db_xref="GeneID:57773"
                     /db_xref="MGI:MGI:1889002"
                     /translation="MRLRPARMLLDGTPFARRRVTSLTSANRKGAARRTCPEAAGGPM
                     ASSAGLALCAQTLVVRGGSRFLAFSTTGSDDDCVFTYDCSTAEKKATPEDKGEDGQPA
                     DTGSDSILASTFSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASED
                     RVLVADKSGDVYSFSVLEPDGCGRLELGHLSMLLDVAVSPDDQFVLTADRDEKIRVSW
                     AAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSSGDGTLRLWEYRSGRQLQCCDLAG
                     LQEPGEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQQLVFRQRLTFP
                     HRVWDVVFEEARGLWVLQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAML
                     EGSVGTDDSFRSLYKATFDNMTSYLKKKEERLQQQLKKKRQRSPFPGSPEQTKKACPG
                     QSALSC"
     exon            478..546
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            547..690
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            691..847
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            848..960
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1021
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1120
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1185
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1369
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1439
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..4046
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2225..2230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="hexamer: AATAAA"
     polyA_site      2245
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="major polyA site"
     regulatory      4019..4024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="hexamer: ATTAAA"
     polyA_site      4046
                     /gene="Wdr4"
                     /gene_synonym="D530049K22Rik; mWH; Wh"
                     /note="major polyA site"
ORIGIN      
        1 gatttgggcc tgcccacctt gccctgatct cgcccttaac tctggtctgt aacctaccaa
       61 aattgcacag ctagaatcat gtcctttgcg tttctggttc gacttgagag acttctgtgt
      121 ttggtgagat ctttgggaca cttgcatgct ttagagaccc cgtccaagag aagccaccta
      181 acctgacagg cagaaaagtc aatcaccaaa ggcaggtgga gggatctaca gcatcagcac
      241 ctccgggcac cgaaatcaaa tgagactacg ccctgcgcgc atgctcctgg acggcacgcc
      301 gttcgcgcgg cgccgagtga catcactgac aagcgccaac aggaagggcg cagcgcgtcg
      361 cacgtgtccg gaggcggcgg gcgggcccat ggcgagctct gcggggctgg cactgtgcgc
      421 ccagacgctg gtggtgcgag gaggcagccg gttcctagcc ttctccacta cgggcagtga
      481 tgatgactgt gtcttcacat acgactgcag tactgcagag aagaaggcca cgccagaaga
      541 taaaggggag gacggacagc ccgcagacac agggagtgac tcgattctgg cgtccacctt
      601 ctccaagtct ggccgctatt ttgctttaac agatgacagt aagcgtctga ttcttttccg
      661 tacaaaacca tggcaatgtc tgagtgtcag gatggtggtg cggaggtgca ccgccctgac
      721 cttcacagcc tcagaggacc gagtcttggt ggctgacaag tctggagacg tctactcctt
      781 ttcggtgctg gagccagatg gatgtggcag gctggagctt gggcacctct ccatgctgct
      841 agacgtggct gtgagtcctg atgaccagtt tgtgcttact gcagaccggg atgagaagat
      901 ccgggtcagc tgggctgctg ccccgcatag catcgagtct ttctgcctgg gacacactga
      961 gtttgtgagc cgcatccttg tagtgcccag tcatcctgaa ctgctgcttt cttcctctgg
     1021 ggatggcacc ctgagactct gggagtacag aagcggtagg cagctgcagt gctgtgacct
     1081 ggccggccta caggagcctg gagagcagcc aggccacaag gggttggccg cgtccaggat
     1141 tgcattctgg ggacaggaga gctatgtggt gcttctgtgt gagtgcgttc ccgtggtctt
     1201 cgtcttccag cttgatgcca gcagacagca gctggtgttc agacagcggc tgactttccc
     1261 tcaccgagtg tgggatgttg tgtttgagga ggcccggggg ctgtgggttc tacaggactg
     1321 ccgtgatgcc cccctggtgc tctggaggcc tgtgggtggt gagtggcagg ctgctccaga
     1381 cggtgctgtg tccccgagac tctgcagcca tctccgtgag agctgggcca tgctggaagg
     1441 ttctgttggt acagatgaca gcttccgcag cctgtacaag gccacctttg acaacatgac
     1501 ctcttacctg aagaaaaagg aagagagact gcagcagcag ctgaagaaga agcggcaaag
     1561 gagccccttc ccagggtccc cggaacagac caaaaaggcg tgcccgggcc agtcagccct
     1621 tagttgctga ccttggacat ggccacagct ctcattcctg aggccctgtg ggcagctctc
     1681 tccctcctcc tccctggttc atccctaggg agggagatca cggcagctca gtccctacca
     1741 ctagctcaac agtccctgat ctccagagtc tgcctttgtc actgagaaca ggctcctgcc
     1801 gggttgcagg gaaggcaggt ggtgttagct tagctaagag tctagagctg tgtggaagca
     1861 ccttcacatt gaaggataca ctggcccaca gcaccacgca tgtctgtcgg ggcccacagt
     1921 gtcgtccctg gggagctcta gggagacctg caggacagga cctgccttca catcattgcc
     1981 gggggctctc caggtggagc tctgtatggc tctgtagctg cttgctgttt tgaggcagat
     2041 tcagttggtg ctgttcgcac tcctgggctg ggctgagcag actgagcaag ctttggggct
     2101 ctgagggtgt gaccttgcca ggccagcctt gttttacagg gaatgatggt gacctcgtgg
     2161 ttctgttgct ctgaactctg catacactgc agaacagaag gaaatgaaca gaaatcacag
     2221 catcaataaa gacaaacttt gcatagcttt ctggaatgtt cagcagcagc ctccatagct
     2281 gatcttaaga cattcaatgt gtgtatgctc ttcaaatgac atggctcttg gtggttagag
     2341 aggggtgttg tccagctctg tctattctcg tgctatttcc cagacgagct gcagcagaga
     2401 ccgtcttggg ttggggagat gagacccgag tttaggtacc acccaaacat acagaacaaa
     2461 ggaaggccat agcctagcat aactgtaacc ctaatactgg agctgcagac tgaggcagcc
     2521 tagcctaaac agtgacattt tgggctctgt gagatcctgt ctcaagatga acagtcgagg
     2581 aagatgccca gtgtcatcgt ctggcctcca cacgcagtag aaatagtctt gtggccacaa
     2641 gcagctatca gtcgaatggg tggtgttcac ggagcagggt gctcacctct gtctaggagc
     2701 taatgtcacg tgttctggat gctgggatgt gtggtcggtc cctggagaca tggatgtgcc
     2761 aactgggctg tgggctgggt cctaactagt cggagataaa tgagagaagt ctgagggccc
     2821 aggaatacag ctttgctgag ccagtgctgc attcgctcag tcctctggtc atgcgtagcc
     2881 tgcgagccag gtagagccca tgcttgcaaa gcaccagtcc tcacagggcc gtcatggccg
     2941 gatggtcagt gcaaggagct gagagactcg aggggtatct gaagtccctg gtgtctgcct
     3001 tgtaggctct gagacagcca cacagacagt gatggggagt ccttgtgtca aaggagagat
     3061 ggtggccttc atggtggggt tattcctaag gtcctggtga gtcacagagc caaacagcat
     3121 agtgagcagc aggcaggccc accccaacaa tcccagtcct gtctccactt ttctcaagcc
     3181 ccagtgttca tgattgtcct atgccacagt gacatctgtg catgtcccag ggtccactta
     3241 cttgtgtgag tgtagccagt ggaagcacta agggtggggt agaggagggt gttgggcctc
     3301 catgtcctac catctggctc tggctttcct ggccctgggg cctgcagtgg ctcctggtcc
     3361 cttaggcata gcttcggagc ctgttaattg gtcccacttt caggggcctt aatgcctgta
     3421 ggttgggtac atagcattat gcttacagga ccctgctagc tagtaggcta gtccacatgc
     3481 ctacacttca tgtggttcca gaaatttctt ccatattagg gtgtgtggag tcccattttt
     3541 attgggacta cacttttatt taaggggatt tgtgtcttac ctggttttag gccagtctga
     3601 cctgggcagt tcatgctatg atttcagctt gtgtcaggat gcctccacct ccagcagcag
     3661 ctccccactt ccactgggat gcccagttgc cagggtcgtt cccaaaaggc tgcccaccag
     3721 tctctgataa gacaccaagg caatttatat gagagagatt gactggcctt gcagttccag
     3781 agggttgggg gccatgctgg tggaagggag gctggagccc cagttgagag ctcccatctt
     3841 ccacaagcag gaggcaggcc ggacacactg gcaatgacac atcaacaagg ccctaagtcc
     3901 taatccttct aaacatctcc aacactgggg accgttccca tccagatcag tgcacacagc
     3961 tctctccttt gtcagtttag ttagtttggc ccctgtactc ccctaaaccc cctgcctcat
     4021 taaacttgta tcttcattcc tcatta
//
