LOCUS       NM_001146022            9681 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus WD repeat and FYVE domain containing 4 (Wdfy4), mRNA.
ACCESSION   NM_001146022
VERSION     NM_001146022.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Ferris,S.T., Liu,T., Chen,J., Ohara,R.A., Ou,F., Wu,R., Kim,S.,
            Murphy,T.L. and Murphy,K.M.
  TITLE     WDFY4 deficiency in NOD mice ameliorates autoimmune diabetes and
            insulitis
  JOURNAL   Proc Natl Acad Sci U S A 120 (13), e2219956120 (2023)
   PUBMED   36940342
  REMARK    GeneRIF: WDFY4 deficiency in NOD mice ameliorates autoimmune
            diabetes and insulitis.
REFERENCE   3  (bases 1 to 9681)
  AUTHORS   MacNabb,B.W., Tumuluru,S., Chen,X., Godfrey,J., Kasal,D.N., Yu,J.,
            Jongsma,M.L.M., Spaapen,R.M., Kline,D.E. and Kline,J.
  TITLE     Dendritic cells can prime anti-tumor CD8+ T cell responses through
            major histocompatibility complex cross-dressing
  JOURNAL   Immunity 55 (6), 982-997 (2022)
   PUBMED   35617964
  REMARK    Erratum:[Immunity. 2022 Nov 8;55(11):2206-2208. doi:
            10.1016/j.immuni.2022.09.015.. PMID: 36351377]
REFERENCE   4  (bases 1 to 9681)
  AUTHORS   Li,Y., Li,J., Yuan,Q., Bian,X., Long,F., Duan,R., Gao,F., Gao,S.,
            Wei,S., Wang,A., Liu,A., Li,X., Sun,W. and Liu,Q.
  TITLE     Deficiency in WDFY4 reduces the number of CD8+ T cells via reactive
            oxygen species-induced apoptosis
  JOURNAL   Mol Immunol 139, 131-138 (2021)
   PUBMED   34482201
  REMARK    GeneRIF: Deficiency in WDFY4 reduces the number of CD8(+) T cells
            via reactive oxygen species-induced apoptosis.
REFERENCE   5  (bases 1 to 9681)
  AUTHORS   Li,Y., Wang,A., Long,F., Gao,F., Gao,S., Wei,S., Liu,A., Li,X.,
            Sun,W., Li,J. and Liu,Q.
  TITLE     Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2
            Cell Differentiation
  JOURNAL   Int Arch Allergy Immunol 182 (11), 1089-1096 (2021)
   PUBMED   34425575
  REMARK    GeneRIF: Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via
            Enhanced Th2 Cell Differentiation.
REFERENCE   6  (bases 1 to 9681)
  AUTHORS   Yuan,Q., Li,Y., Li,J., Bian,X., Long,F., Duan,R., Ma,X., Gao,F.,
            Gao,S., Wei,S., Li,X., Sun,W. and Liu,Q.
  TITLE     WDFY4 Is Involved in Symptoms of Systemic Lupus Erythematosus by
            Modulating B Cell Fate via Noncanonical Autophagy
  JOURNAL   J Immunol 201 (9), 2570-2578 (2018)
   PUBMED   30257884
  REMARK    GeneRIF: Genetic silencing of WDFY4 in B cells increased lipidation
            of LC3 independent of p62 and Beclin1, which are essential proteins
            of canonical autophagy. In vivo and in vitro data suggest that
            WDFY4 facilitates noncanonical autophagic activity. These findings
            provide a novel functional link underlying the mechanism of
            systemic lupus erythematosus in which WDFY4 influences B cell fate
            via noncanonical autophagy.
REFERENCE   7  (bases 1 to 9681)
  AUTHORS   Pritsker,M., Doniger,T.T., Kramer,L.C., Westcot,S.E. and
            Lemischka,I.R.
  TITLE     Diversification of stem cell molecular repertoire by alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 102 (40), 14290-14295 (2005)
   PUBMED   16183747
REFERENCE   8  (bases 1 to 9681)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   9  (bases 1 to 9681)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
REFERENCE   10 (bases 1 to 9681)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC154239.3,
            AC154206.2, AC165262.2 and AC133585.11.
            
            On Mar 23, 2013 this sequence version replaced NM_001146022.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN00849374, SAMN00849375
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               AC154239.3         110512-110710
            200-449             AC154206.2         12845-13094
            450-564             AC154206.2         20033-20147
            565-671             AC154206.2         21688-21794
            672-806             AC154206.2         23131-23265
            807-996             AC154206.2         24045-24234
            997-1186            AC154206.2         26133-26322
            1187-1344           AC154206.2         28706-28863
            1345-1797           AC154206.2         30410-30862
            1798-1902           AC165262.2         199542-199646       c
            1903-2093           AC165262.2         197750-197940       c
            2094-2671           AC165262.2         190414-190991       c
            2672-2765           AC165262.2         183058-183151       c
            2766-2980           AC165262.2         166717-166931       c
            2981-3157           AC165262.2         164835-165011       c
            3158-3234           AC165262.2         164318-164394       c
            3235-3456           AC165262.2         163202-163423       c
            3457-3635           AC165262.2         161607-161785       c
            3636-3867           AC165262.2         161120-161351       c
            3868-4013           AC165262.2         159742-159887       c
            4014-4125           AC165262.2         158717-158828       c
            4126-4316           AC165262.2         158119-158309       c
            4317-4469           AC165262.2         156231-156383       c
            4470-4543           AC165262.2         154997-155070       c
            4544-4705           AC165262.2         153146-153307       c
            4706-4797           AC165262.2         152483-152574       c
            4798-4897           AC165262.2         150454-150553       c
            4898-5103           AC165262.2         148006-148211       c
            5104-5273           AC165262.2         147067-147236       c
            5274-5399           AC165262.2         145158-145283       c
            5400-5564           AC165262.2         140854-141018       c
            5565-5714           AC165262.2         135421-135570       c
            5715-5953           AC165262.2         133462-133700       c
            5954-6075           AC165262.2         130676-130797       c
            6076-6261           AC165262.2         127794-127979       c
            6262-6398           AC165262.2         126038-126174       c
            6399-6476           AC165262.2         104460-104537       c
            6477-6680           AC165262.2         99679-99882         c
            6681-6732           AC165262.2         98368-98419         c
            6733-6899           AC165262.2         95771-95937         c
            6900-7048           AC165262.2         86161-86309         c
            7049-7169           AC165262.2         80522-80642         c
            7170-7255           AC165262.2         77459-77544         c
            7256-7318           AC165262.2         75756-75818         c
            7319-7361           AC165262.2         47127-47169         c
            7362-7481           AC165262.2         46100-46219         c
            7482-7599           AC165262.2         43845-43962         c
            7600-7709           AC165262.2         42871-42980         c
            7710-7863           AC165262.2         34304-34457         c
            7864-7955           AC165262.2         32369-32460         c
            7956-8168           AC165262.2         31369-31581         c
            8169-8319           AC165262.2         30750-30900         c
            8320-8504           AC165262.2         28792-28976         c
            8505-8663           AC165262.2         28037-28195         c
            8664-8843           AC165262.2         24687-24866         c
            8844-9026           AC165262.2         23578-23760         c
            9027-9111           AC165262.2         21753-21837         c
            9112-9223           AC133585.11        172267-172378       c
            9224-9681           AC133585.11        171072-171529       c
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.44 cM"
     gene            1..9681
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /note="WD repeat and FYVE domain containing 4"
                     /db_xref="GeneID:545030"
                     /db_xref="MGI:MGI:3584510"
     exon            1..199
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            200..449
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..9290
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /codon_start=1
                     /product="WD repeat- and FYVE domain-containing protein 4"
                     /protein_id="NP_001139494.1"
                     /db_xref="CCDS:CCDS49441.1"
                     /db_xref="GeneID:545030"
                     /db_xref="MGI:MGI:3584510"
                     /translation="MEAEDLSKTEDRPEDPGFQNEGQSPAVKPSFSLEGQSPGPSVLW
                     DMLEQKFLDYQQLMPRNPEERRKNLLSLLPLFLKAWEHSVGIICFRSLQRLAEDVSDQ
                     LAQEIQQALAGKPAEQARAAAGQLLQWKSDADQDGNLLLKSVYVLTGTDSETLGRVVD
                     SGLPALLLQCLYLFFAFPVEKDDLLESDVQGQRMFVQMLLNICSESQGLEGLLSGSEL
                     QSLLIATTCLREHSCLFWKQPTFCVLRAISKAQSPSVIQYLRTADCVRLSVQNLSKLA
                     DTLPAPEVSEAVSLILNFVRDSYPISSALLLEFENGEGYPLLLKVLLRYNGLTQGVVE
                     PHLEELIELVMWLTTCGRSELKVFDSVTYPQLEGFKFHQEASGVTVKNLQAFQVLQNL
                     FHRASDSVLCIQVLLAIKTMWAWNPRNFFLLEWTLQPISQFAEIIPLKPTPVQEHFFQ
                     LLETLVFKLLYVPHEVLAKVQRLIKESSELSCTLVALRSILRITASDRLFTDIFRDSG
                     LLGLLLAQLRKQAKIMRKSGNKECSPDVQDPERELTYVMLSTVVTLLQGSVRNAVVLK
                     DHGMVPFIKIFLDDECYRGPSLSILEQLSVINAEEYMSIIVGALCSSTQGELQLKLDL
                     LKSLLRILETPKGHAAFRVSSGFNGLLSLLSDLEGSLQVPEVTTCGAVSPSQTLELVL
                     HTLCVVSAALHLDPVNEHFFRSNGLFEKLAEDLCLLGCFGTPEEERTRWDSSSDMKAR
                     PFMDLLSCAFSSSCQFPPRLQSCLQILSFLESMASGTLHLRGDLMEPARAGQEPSVDA
                     QKAEAGGRQGKFKQWPDMEDRMDEGDVMIMHPGIICIMVRLLPRLYLEDHPQLSEEIQ
                     CSVARHLLSLVKSEKNRQVMCEAGMLRTLMTFCPRTLSTGGSDLHSILIRIFEKLGSQ
                     AIEPDVLSPRPGGSQALRPSWASQYSATALQTTLSLISMTSPRNLQPQRAALTPSFVE
                     FDMSSEGYGCLFTPTLSTVMGTSTEHSISGGTGSGAPRPFPPPGGLTFSCWFLISRQA
                     NVMEGHPLRFLTLVRHLARTEQPFVCFSISLCMDDLSLVVSTEEKEFQPLDAMEPEDE
                     AEPSAGRQLQVRCSQLLTCGQWYHLAVVVSKEMKRNCSVTTYLDGQAIGSAKMLYIQA
                     LPGSFFSMDPSSFVDVYGYIGTPRVWKQKSSLTWRLGPAYLFEEDISADTLALIIKLG
                     PRYCGNFQAVHLQGEDPDGEATPLIAEERVSFGLYVPSSSITSIMNIRNTYNEVDSRL
                     IAKEMNISSRDNATPVFLLRNCAGHLSGPLRTLGAVAVGQLGVRVFHSSPAASSLDYI
                     GGPAILLGLISLATDDHTMYAAMKVLHSVLTSNAMCDYLMQHICGYQILAFLLRKKTS
                     FLNHRIFQLILSVAGTAELGFRPSAVTNMCIFQHVLCNFELWTNTADNLELTLFSHLL
                     EILQSPREGPRNAEVAHQAQLLPKLLFLFNEPSLALSKVSTIIAILGCQLKGHFNIQD
                     LLRVGLFVIYTLKPSSVNERQICLDGAQDPSRPAGSQTSGKAIWLRNQLLEMLFGVIS
                     SSQLHLTSELKEQVFLSLGPDWFLLLLQGHLHPSTTTLALKLLLYFLSSPPLRGRFRD
                     GLSAGCWVENCMDGVDIVMDNLKSRPAVPDQSPCLLPGFRVLNDFLAYHVLIPEVYLI
                     VSSFFLQTPLTELTNGPRENLDLMLQWLLQKHHQQEVLQAGLCIEGALLLLGMLKAIM
                     NQPPAGSGDGAWEQTLPSSVLQFLRLVHRSYPQDSAWRTPDFLQTVAIITFPLETQKE
                     PLPNAEAGAVPSLASVSYFTQKLVEKIYSGVFSADPRHILLFITEHIIAVIENPSSQK
                     DTVMSALYSSLNKVILHCLSKPQQSLSECLGLLTILDFLQEHWDIIFATYNSNVSFLL
                     CLMHCLLLLNARSYPEGFGLEPKPRITPYHQVFLSPNEEVKDKKEEGLPSLGDVQHSI
                     QKSVRALWQQLVAQRRQTLEDAFKIDLSVKAGEIEVKIEEITPLWEETMLRAWQHYLA
                     SEKKSLASRSSVMHHSKVTSWSGSLSSAMRLMPGRQAKDPECRAEDFVSCIENYRRKG
                     QELYASIYKDYVQRRKSGSIKAATAWARMREQLFGELGLWGQMTESTRCSRWELDGRE
                     GPARMRKRIRHLLAWEPLNLGYKESQEGKGDVSQTNTGNQDELTTEEAESRPDEVGVD
                     CTQLTFFPALHESLHSEDFLELCRERQVILQELLDGEKVSQKVPMVIVQGHLVSEGIL
                     LFGQHHFYICENFTLSPTGDVYCTHHCLSNISDPFIFNMCSKDRSSDHYSCQRHAYSD
                     LRELRQARFLLQDIALEIFFQNGYSKLLVFYNSDRSKALKSFSTFQPSLKGKGTTEDP
                     FNLRKHPGFDRTMLQRWQKREISNFEYLMYLNTLAGRTYNDYMQYPVFPWVLADYTSE
                     MLNLTNPKTFRDLSKPMGAQTKERKLKFTQRFKDVEKIEGDMTVQCHYYTHYSSAIIV
                     ASYLVRMPPFTQAFCSLQGGSFDVADRMFHSVKSTWESASKENMSDVRELTPEFFYLP
                     EFLTNCNAVEFGCMQDGTTLGDVQLPPWADGDPRKFISLHRQALESDFVSSNLHHWID
                     LIFGYKQQGPAAVEAVNTFHPYFYGDRIDLGSITDPLIKSTILGFISNFGQVPKQIFT
                     KPHPSRNTTGKNPGPGKDASTPVGLPGHSQSFLHSLPALRPSQVTVKDMYLFSLGSES
                     PKGAIGHIVPTEKSILAVEKNKLLMPPLWNRTFSWGFDDFSCCLGSYGSDKILMTFEN
                     LAAWGPCLCAVCPSPTMIVTSGASAVVCIWELSLVKGRPRGLKLRQALYGHTQAVTCL
                     TASVTFSLLVSGSQDRTCILWDLDHLSRVACLPVHREGISAIAISDVSGTIVSCAGAH
                     LSLWNVNGQPLASITTAWGPEGTITCCCIVEGPAWDASHVIITGSKDGMVRIWKTEDV
                     KMPVPRQAVMEEPSTEPLSPRGHKWAKNLALSRELDVSVALSGKPSKASPAVTALAIT
                     RNQSKLLVGDEKGRIFCWSADG"
     exon            450..564
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            565..671
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            672..806
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            807..996
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1186
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1344
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1797
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1902
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2093
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2671
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2765
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2980
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3157
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3234
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            3235..3456
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3635
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3867
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..4013
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4014..4125
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4126..4316
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4317..4469
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4470..4543
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4544..4705
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4797
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4798..4897
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            4898..5103
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5104..5273
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5274..5399
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5400..5564
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5565..5714
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5715..5953
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            5954..6075
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6076..6261
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6262..6398
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6399..6476
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6477..6680
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6681..6732
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6733..6899
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            6900..7048
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7049..7169
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7170..7255
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7256..7318
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7319..7361
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7362..7481
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7482..7599
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7600..7709
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7710..7863
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7864..7955
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            7956..8168
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            8169..8319
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            8320..8504
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            8505..8663
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            8664..8843
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            8844..9026
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            9027..9111
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            9112..9223
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     exon            9224..9681
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9640..9645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /note="hexamer: ATTAAA"
     polyA_site      9665
                     /gene="Wdfy4"
                     /gene_synonym="Gm18810"
                     /note="major polyA site"
ORIGIN      
        1 aggagtctgc cacagctggg gaaaactaac tctggcttgt gccactgtca aggtatagaa
       61 cgggacactg actgagacgc tcctctcata gccctggtgt agcttgtgaa gggtggacca
      121 cccaccctgg ccttcggggc agctgggcag acctggtctt gcccttagct ggccccaccc
      181 ttgcctgccc caggatctga tctgcttcgc caacgatgga ggctgaagat ctttcaaaga
      241 ctgaggacag acccgaggac ccaggtttcc aaaatgaagg gcaatcccct gctgtgaagc
      301 ccagtttttc actagaaggg cagtcgcccg gccccagtgt cctctgggat atgcttgaac
      361 agaaattcct ggactatcag cagctaatgc ccaggaaccc agaagaacgt cgcaagaact
      421 tgctgagcct tctccccctc ttcctaaagg cttgggaaca ttctgtgggt attatctgct
      481 ttcgaagtct ccaaaggctg gcagaagatg tgtctgacca acttgcccag gaaatacaac
      541 aggcacttgc tgggaagccc gcagagcaag cccgggccgc agctgggcag ctactgcagt
      601 ggaagagtga cgcggatcag gatggcaacc tgctcctaaa gtcagtgtat gtgctcacgg
      661 ggacagactc ggagacactg ggcagagtgg tggattcagg gcttccagcc ctgctccttc
      721 agtgcctgta tctcttcttc gcttttcctg tggaaaagga tgaccttttg gagagcgatg
      781 ttcaagggca gaggatgttt gtgcagatgt tgttaaacat ttgcagcgaa tctcagggtc
      841 tggagggact tctctcagga agcgagctcc agtctctact gatcgccacc acctgtctga
      901 gggaacacag ctgcttgttc tggaaacagc ctaccttctg tgtgctgcgg gcgatctcca
      961 aggctcagag ccccagcgtc atccaatatc tgcgaactgc agactgtgtc cggctctcag
     1021 ttcagaacct ctccaagttg gcagacactc tccccgcgcc ggaggtgagc gaggctgtga
     1081 gcctcatcct gaactttgtg agagactcct accccatctc ctctgcgctg ctcctggagt
     1141 ttgagaacgg ggagggctac cctcttctcc tcaaggtact gctgcggtat aacgggctga
     1201 cccagggtgt ggtggagccc catctggagg agctcatcga gctggtgatg tggctgacaa
     1261 cctgtgggag gtcagagctg aaggtgtttg acagcgtcac ttatcctcag cttgaaggct
     1321 tcaagttcca tcaggaggct tctggagtga ctgttaagaa tctccaggcc ttccaggtcc
     1381 tgcagaatct tttccacaga gccagtgact ctgtcctttg cattcaagtg cttttggcca
     1441 tcaagaccat gtgggcctgg aatcctcgca atttcttcct gctggagtgg accctgcaac
     1501 ccatctcaca gtttgcagag atcattcccc tgaagccaac cccagtgcag gagcactttt
     1561 tccagctgtt ggagactctg gtgttcaagc tgctctatgt gccccacgag gtcctggcta
     1621 aggtgcagcg tctgatcaag gagagctcgg agctctcctg taccctcgtg gccttgcgga
     1681 gcatcctcag gatcacagcc agtgaccggc tcttcacaga catcttccgg gactcgggac
     1741 tactaggtct gctgctggcc cagctgcgga aacaggccaa gatcatgagg aagtcaggaa
     1801 acaaagagtg cagccctgat gtgcaggatc ctgagaggga actcacctac gtgatgctga
     1861 gcactgtggt cacacttctg caaggctctg ttcgaaatgc agttgtccta aaagaccatg
     1921 gcatggtgcc cttcatcaag atcttcctgg atgatgagtg ctaccggggc ccctcgctca
     1981 gcatcttgga gcagctctca gtcatcaatg cagaggagta catgagcatc attgtgggag
     2041 ctttgtgttc gtccactcag ggggagctgc agctgaagct ggatcttctc aagtctctgc
     2101 tccggatcct ggagaccccc aagggccatg ctgcattccg cgtctccagt ggcttcaatg
     2161 ggctgctgtc cctgctttcc gacctggagg ggtccctcca agtacctgaa gtgacaacat
     2221 gtggtgcagt gtcccccagc cagaccctgg agctggtgtt acacaccctt tgtgtagtgt
     2281 ctgctgctct gcacttggac cctgtcaacg agcacttctt caggagcaat ggactctttg
     2341 agaagctggc tgaggacctc tgtctgctgg gctgttttgg gaccccagag gaggagcgca
     2401 ctcgatggga ctcttcatct gacatgaaag ccaggccctt catggatttg ctgagctgtg
     2461 ccttctcctc cagctgccag ttcccaccca ggctgcagag ctgcttacag attctcagct
     2521 tcctagagag catggccagt ggtaccctcc acctgcgggg agacctgatg gagccagcca
     2581 gggctgggca ggagccttct gtggatgctc aaaaggcaga ggctggtggc cgccaaggca
     2641 aattcaagca gtggccagac atggaggaca ggatggatga aggtgacgtc atgatcatgc
     2701 atcctgggat catttgcatc atggtgagac tgctgcctcg tctatacctc gaagatcacc
     2761 cacagctttc tgaggagatc cagtgctctg tggcccgcca ccttttgtcc ctggtgaaat
     2821 cggagaagaa ccgccaggtc atgtgtgagg cagggatgct ccggacactc atgacatttt
     2881 gccccaggac cctcagcact ggtggcagtg acctgcactc gattctcatc aggatctttg
     2941 aaaagcttgg atcccaagcc atagagccag atgtgctaag cccacgtcct ggagggtctc
     3001 aggccctcag gccctcatgg gcctcccaat attcggccac agccctccag acaacactga
     3061 gcctcatctc catgacctcc cctcgaaatc tgcagcctca gagggcggcc ctgacaccat
     3121 catttgtgga atttgacatg tcttcagaag gttatggctg tctgtttact ccgaccttgt
     3181 ctacagtcat ggggaccagc acagagcact ccatctctgg agggactgga tcaggagctc
     3241 ccaggccgtt ccctccgcct ggaggtctca ccttctcctg ttggtttctg atcagccggc
     3301 aggccaatgt catggagggc cacccactgc gcttcctaac cctggtgcgc cacctggcca
     3361 ggacagagca gccctttgtc tgcttctcca tcagcctctg catggatgac ctctccttgg
     3421 ttgtgtccac agaagagaaa gagttccagc ccctggatgc catggagcct gaggatgagg
     3481 cagagccctc tgcaggccgc cagctccagg tcaggtgcag ccagctgctg acatgtggtc
     3541 agtggtatca cctggctgtg gtggtcagca aagaaatgaa gaggaattgc agtgttacca
     3601 cgtacctgga tgggcaagcc attggttcag ccaagatgtt gtatattcag gccttaccgg
     3661 ggtctttttt ttcaatggat ccctcatcat ttgtggatgt ttatggctat attgggaccc
     3721 ccagagtttg gaaacagaaa tcatcgctaa cctggcgtct cgggcctgca tacctctttg
     3781 aagaagacat atcagcggat actctagcac ttattatcaa actcggcccg aggtactgtg
     3841 gtaactttca agctgtgcac cttcaaggag aagacccaga tggcgaagcc acgcccctca
     3901 tcgcggagga aagggtttcc tttggtcttt atgtacccag ctcttctatc acaagcataa
     3961 tgaacatcag aaacacctac aatgaggtgg acagccgctt gatcgccaag gagatgaata
     4021 tttcctcccg tgacaatgcc acacctgtgt tcttgctgag aaactgtgcc gggcacctct
     4081 cagggcctct gcggactctc ggggctgttg ctgtgggcca gttaggggta agggtgttcc
     4141 attccagccc tgctgccagc agcctggact acattggagg acctgccatc ctcctaggcc
     4201 tcatctccct ggcaacagat gaccacacca tgtatgcagc tatgaaggtc ctgcactcgg
     4261 tcctgaccag caatgccatg tgtgattacc tgatgcagca catctgtggg taccagatct
     4321 tagcctttct cctgcggaag aagacatcct ttctaaacca ccgcattttc cagttgatcc
     4381 tctcagtggc tggcacagcg gagctgggtt tcaggccgtc tgctgtcact aacatgtgta
     4441 tcttccagca tgtgctctgc aattttgagc tctggacaaa cactgcagac aacctggagc
     4501 tcacgctctt ttctcatctc ctggagatcc tgcagtcacc aagggaagga cccaggaacg
     4561 ctgaggtagc ccaccaggca cagctgctac ccaagctctt gtttctcttc aatgagccga
     4621 gtctggctct gtccaaggtc tccaccatca ttgccattct gggctgtcag ctgaagggcc
     4681 acttcaacat ccaggactta ctcagggttg ggctgtttgt catatacacc ctcaagcctt
     4741 catcagtgaa cgagaggcag atctgcctgg atggagccca ggacccctcc agacccgctg
     4801 gaagccaaac atctggaaag gcaatctggc ttagaaacca gttgctggag atgctgtttg
     4861 gtgtaatatc ttcctcccaa cttcatctga cctctgagtt aaaggaacag gtgtttctga
     4921 gcctggggcc cgactggttc ctgctcctcc tgcagggtca cctgcatccc agcaccacca
     4981 cgttggcatt gaagctgctg ctgtactttc tgtcaagccc tcccctccgt gggcgattta
     5041 gggatggcct atctgctgga tgctgggtag agaactgcat ggacggggtg gacattgtga
     5101 tggacaacct gaagagccgc cctgcagtgc ctgaccagag cccctgcctg cttcctgggt
     5161 tccgggtctt gaatgacttt ctggcctacc atgttctcat tccggaagtg tacctcatcg
     5221 tatcttcctt cttcctccag acaccactta cagagctgac caatgggccc agagagaact
     5281 tggatttgat gcttcagtgg cttcttcaga aacatcatca gcaagaagtc ctccaggctg
     5341 ggctgtgcat agaaggtgct ctgctactcc tggggatgct gaaagccatc atgaatcagc
     5401 cccctgcagg ttcaggagac ggcgcctggg agcagacact ccccagcagt gttcttcagt
     5461 tcctccgcct ggtgcaccgc tcctaccccc aggactcagc gtggaggacc cctgacttcc
     5521 tccagactgt ggccattatc actttccccc tggaaaccca gaaggagcct ctgccaaatg
     5581 cagaggctgg tgctgtgcct tcccttgcaa gcgtttccta cttcacccag aagctggttg
     5641 agaagatata tagtggggtg ttctcagcgg atcccaggca catcctcctc ttcatcacag
     5701 agcacatcat agcggtcatc gagaaccctt cttcccaaaa ggacactgtc atgagtgcat
     5761 tgtacagcag tttgaataaa gtcatcctgc attgcctctc caagccccag cagtccctgt
     5821 ctgagtgcct tggcctcctc accatcctgg acttcctgca ggagcactgg gacatcatct
     5881 ttgccaccta caattccaat gtcagcttcc tcctgtgcct catgcattgt cttttgctgc
     5941 tgaatgccag gagttatcca gaaggatttg gattggagcc caagcccaga attactcctt
     6001 atcaccaagt cttcctctcc ccaaatgaag aagtgaaaga taagaaggag gagggcctcc
     6061 caagtctggg tgatgttcag cacagcatcc agaagtcagt gcgggcgctg tggcagcagc
     6121 tggtggcaca gaggcgacag accctggagg atgccttcaa gatagatctc tccgtgaagg
     6181 ctggggaaat cgaagtgaag atcgaagaga tcaccccact ttgggaggag acgatgctca
     6241 gagcctggca gcattactta gcatccgaga agaagtcact ggccagccgt tcaagtgtca
     6301 tgcaccacag caaagtgact tcatggagtg ggagcctatc ctcagccatg agactgatgc
     6361 cagggcgaca ggcaaaggat cctgagtgca gagcagagga ttttgtgtcc tgcatcgaga
     6421 attacagaag gaaaggacag gagctgtatg catctatata caaggactat gtgcagagga
     6481 ggaaaagtgg cagcatcaag gcagccacag cctgggccag gatgcgggaa cagctctttg
     6541 gggagctggg attgtggggc caaatgacag aatccacacg ctgctcccgg tgggaactgg
     6601 atgggaggga aggaccggct cgcatgagga aacgcatcag acacctgtta gcatgggagc
     6661 ccctgaacct aggatacaag gagagccaag aaggaaaggg tgatgtttct cagacaaata
     6721 ctggaaacca agatgagctg acaacagagg aagctgagag caggccggat gaggtgggag
     6781 tggattgcac acaactgacc ttctttcctg ctttgcatga aagcctgcac tcagaagact
     6841 tcttggaatt gtgccgggaa agacaagtca ttttacaaga gcttcttgat ggagaaaagg
     6901 tgtcacagaa ggtccccatg gtgattgtgc agggccacct ggtctcagag gggatcctgc
     6961 tctttggcca gcatcacttc tacatctgtg agaactttac tctgtctcct acaggcgatg
     7021 tctactgcac ccaccactgc ttatccaata tcagtgaccc cttcattttc aacatgtgca
     7081 gcaaagaccg gtcctctgac cattactcct gccagcgcca tgcctacagt gacctcagag
     7141 agctccggca ggcccgcttt ctccttcagg atattgccct ggagatcttc ttccaaaacg
     7201 ggtattccaa gctcttagtc ttctataaca gcgaccggag taaagccttg aaaagcttta
     7261 gcacttttca acccagcttg aaagggaaag gcaccacaga ggaccccttc aacctaagga
     7321 aacacccagg cttcgatagg accatgctgc agaggtggca gaaaagggag atcagcaact
     7381 ttgaatacct catgtacctc aacacactgg ccggaaggac ctacaatgac tacatgcagt
     7441 atcccgtgtt tccctgggtc ctcgctgact acacctcaga gatgttgaac ttgacgaatc
     7501 ccaagacttt ccgggatctt tctaagccaa tgggggctca gaccaaggaa aggaagttga
     7561 agtttaccca gaggtttaaa gatgttgaaa agattgaagg agacatgacc gtgcagtgcc
     7621 actactacac ccactattcc tcagccatca ttgtcgcttc ctacttggtc cgaatgccac
     7681 cattcacgca ggccttctgc tccttacagg gcggaagctt tgatgtggct gatagaatgt
     7741 tccacagtgt aaagagcacg tgggagtctg cctccaaaga gaacatgagc gatgtcaggg
     7801 agctgacacc tgaattcttc tacctgcccg agtttttaac caactgtaat gcagtggagt
     7861 ttggctgcat gcaggatgga acgacactgg gggatgtgca gcttcctccc tgggctgacg
     7921 gggatccgag gaaattcatc agcttgcaca gacaggctct ggaaagtgac ttcgtcagca
     7981 gcaacctcca ccactggata gacctaattt ttgggtataa gcagcagggg ccggctgctg
     8041 tagaggcagt gaacactttc cacccctact tctacggtga tagaatagac ctgggcagca
     8101 tcactgaccc gctgatcaag agcaccatcc tgggcttcat cagcaacttt ggacaggtgc
     8161 ccaagcagat cttcactaaa ccccacccat ccagaaacac cacagggaaa aacccagggc
     8221 ctggaaagga tgcttccacc cctgtaggcc tcccaggcca ctcacagtcc ttcctccaca
     8281 gcctgccagc actgagaccc tctcaggtca cagtcaaaga tatgtacctt ttctctctag
     8341 ggtcggaatc ccccaaaggg gccatcggcc acatcgtccc tactgagaag tcaatcctgg
     8401 cagtggagaa gaacaagctg ctgatgcccc ctctctggaa caggaccttc agctggggct
     8461 ttgatgactt cagttgctgc ctggggagct acggctctga caagatcctg atgacctttg
     8521 agaacctggc tgcctggggt ccctgtctgt gcgctgtatg cccttccccc acgatgatcg
     8581 tcacatccgg ggccagcgca gtggtgtgca tctgggagct gagcctggtc aaaggtcgcc
     8641 cgagaggtct gaaactccga caggccttgt atggacacac tcaggcggtc acatgtctga
     8701 cagcctctgt caccttcagc ctcctggtga gcggatccca ggatcgcact tgtatcctgt
     8761 gggacctgga ccacctctct cgtgtggcct gcctgcctgt ccaccgggaa ggcatctcag
     8821 ccattgccat cagtgatgtc tcgggaacca ttgtctcctg tgccggagcc cacttgtccc
     8881 tgtggaatgt caatggacag cccctggcca gtattaccac agcctggggc ccagaaggaa
     8941 ccataacgtg ctgctgcata gtagaggggc cagcgtggga tgcaagccac gtgatcatca
     9001 cggggagtaa ggacggaatg gttcggattt ggaagacaga ggacgtgaag atgcctgttc
     9061 ccaggcaggc agtgatggag gagccctcca cggagcccct aagccccaga ggtcacaagt
     9121 gggccaagaa tcttgccctg agccgagagc tggatgtcag tgttgctctg agtggcaagc
     9181 ccagcaaggc aagtcctgct gtgacagctc tggccatcac taggaaccag agcaagctct
     9241 tggttggcga tgagaagggg cgaatcttct gctggtctgc tgatgggtag gagacaagga
     9301 gctggaggga ccgacctgaa agcctggagc cctggggtcg gcagcaacag gctacaggca
     9361 caagatgatg tgtagcctgg gctgcttaac cagagcaagt tttggggggg ctccactcca
     9421 cacagttctc aaggagtccc tgatggtttg caccgtgtac cctaaacatg tctgtagtct
     9481 atgggacttc tgtaagaagg atcttggtag acactgatgc tggaaactga cgctgcatgg
     9541 gaaatttcta cgtggctcac ttcaccaagg cttattgcac tgggaaaaga agagggggtg
     9601 ggtattggtt catggaaacc accccactgt ctttatttta ttaaaactcc attttcctaa
     9661 tggaaggctg cttgagcgtt a
//
