LOCUS       NM_028459               4348 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus WASP like actin nucleation promoting factor (Wasl),
            transcript variant 1, mRNA.
ACCESSION   NM_028459 XM_133026
VERSION     NM_028459.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Feng,X., Molteni,H., Gregory,M., Lanza,J., Polsani,N., Gupta,I.,
            Wyetzner,R., Hawkins,M.B., Holmes,G., Hopyan,S., Harris,M.P. and
            Atit,R.P.
  TITLE     Apical expansion of calvarial osteoblasts and suture patency is
            dependent on fibronectin cues
  JOURNAL   Development 151 (7) (2024)
   PUBMED   38602508
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Kim,H.S., Lee,Y.S., Dong,S.M., Kim,H.J., Lee,D.E., Kang,H.W.,
            Kim,M.J. and Park,J.S.
  TITLE     Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes distant
            metastasis in pancreatic ductal adenocarcinoma via activation of
            LOXL2
  JOURNAL   Oncol Res 32 (4), 615-624 (2024)
   PUBMED   38560567
  REMARK    GeneRIF: Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes
            distant metastasis in pancreatic ductal adenocarcinoma via
            activation of LOXL2.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4348)
  AUTHORS   Bhanja,A., Seeley-Fallen,M.K., Lazzaro,M., Upadhyaya,A. and Song,W.
  TITLE     N-WASP-dependent branched actin polymerization attenuates B-cell
            receptor signaling by increasing the molecular density of receptor
            clusters
  JOURNAL   Elife 12, 87833 (2023)
   PUBMED   38085658
  REMARK    GeneRIF: N-WASP-dependent branched actin polymerization attenuates
            B-cell receptor signaling by increasing the molecular density of
            receptor clusters.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4348)
  AUTHORS   Kalailingam,P., Verma,A., Lee,Y.H. and Thanabalu,T.
  TITLE     Conditional Knockout of N-WASP Enhanced the Formation of
            Keratinizing Squamous Cell Carcinoma Induced by KRasG12D
  JOURNAL   Cancers (Basel) 15 (18), 4455 (2023)
   PUBMED   37760426
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4348)
  AUTHORS   Frugtniet,B.A., Ruge,F., Sanders,A.J., Owen,S., Harding,K.G.,
            Jiang,W.G. and Martin,T.A.
  TITLE     nWASP Inhibition Increases Wound Healing via TrKb/PLCgamma
            Signalling
  JOURNAL   Biomolecules 13 (2), 379 (2023)
   PUBMED   36830748
  REMARK    GeneRIF: nWASP Inhibition Increases Wound Healing via TrKb/PLCgamma
            Signalling.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4348)
  AUTHORS   Otsuki,M., Itoh,T. and Takenawa,T.
  TITLE     Neural Wiskott-Aldrich syndrome protein is recruited to rafts and
            associates with endophilin A in response to epidermal growth factor
  JOURNAL   J Biol Chem 278 (8), 6461-6469 (2003)
   PUBMED   12477732
  REMARK    GeneRIF: This protein is recruited to rafts and associates with
            endophilin A in response to epidermal growth factor.
REFERENCE   7  (bases 1 to 4348)
  AUTHORS   Snapper,S.B., Takeshima,F., Anton,I., Liu,C.H., Thomas,S.M.,
            Nguyen,D., Dudley,D., Fraser,H., Purich,D., Lopez-Ilasaca,M.,
            Klein,C., Davidson,L., Bronson,R., Mulligan,R.C., Southwick,F.,
            Geha,R., Goldberg,M.B., Rosen,F.S., Hartwig,J.H. and Alt,F.W.
  TITLE     N-WASP deficiency reveals distinct pathways for cell surface
            projections and microbial actin-based motility
  JOURNAL   Nat Cell Biol 3 (10), 897-904 (2001)
   PUBMED   11584271
REFERENCE   8  (bases 1 to 4348)
  AUTHORS   Lommel,S., Benesch,S., Rottner,K., Franz,T., Wehland,J. and Kuhn,R.
  TITLE     Actin pedestal formation by enteropathogenic Escherichia coli and
            intracellular motility of Shigella flexneri are abolished in
            N-WASP-defective cells
  JOURNAL   EMBO Rep 2 (9), 850-857 (2001)
   PUBMED   11559594
REFERENCE   9  (bases 1 to 4348)
  AUTHORS   Fukuoka,M., Suetsugu,S., Miki,H., Fukami,K., Endo,T. and
            Takenawa,T.
  TITLE     A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding
            protein, WISH, induces Arp2/3 complex activation independent of
            Cdc42
  JOURNAL   J Cell Biol 152 (3), 471-482 (2001)
   PUBMED   11157975
REFERENCE   10 (bases 1 to 4348)
  AUTHORS   Modregger,J., Ritter,B., Witter,B., Paulsson,M. and Plomann,M.
  TITLE     All three PACSIN isoforms bind to endocytic proteins and inhibit
            endocytosis
  JOURNAL   J Cell Sci 113 Pt 24, 4511-4521 (2000)
   PUBMED   11082044
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC058642.1, AJ318416.1, AK143969.1 and CX567238.1.
            
            On Nov 17, 2009 this sequence version replaced NM_028459.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC055045.1, AK143969.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               BC058642.1         1-282
            283-1924            AJ318416.1         1-1642
            1925-4325           AK143969.1         1906-4306
            4326-4348           CX567238.1         667-689
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.28 cM"
     gene            1..4348
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /note="WASP like actin nucleation promoting factor"
                     /db_xref="GeneID:73178"
                     /db_xref="MGI:MGI:1920428"
     exon            1..460
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..1858
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neural Wiskott-Aldrich syndrome protein; Wiskott-Aldrich
                     syndrome-like; actin nucleation-promoting factor WASL"
                     /codon_start=1
                     /product="actin nucleation-promoting factor WASL isoform
                     1"
                     /protein_id="NP_082735.2"
                     /db_xref="CCDS:CCDS19945.1"
                     /db_xref="GeneID:73178"
                     /db_xref="MGI:MGI:1920428"
                     /translation="MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQ
                     LYAADRNCMWAKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRG
                     YFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVD
                     IKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNT
                     GFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPP
                     PPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPP
                     PPNRMYPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGD
                     HQVPAPSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQ
                     ESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED"
     exon            461..595
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            596..682
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            683..779
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            780..803
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            804..972
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1015
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1169
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1687
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1796
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..4333
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4307..4312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /note="hexamer: AATAAA"
     polyA_site      4328
                     /gene="Wasl"
                     /gene_synonym="2900021I12Rik; 3110031I02Rik; N-WASP"
                     /note="major polyA site"
ORIGIN      
        1 ctcgcccgcc cccgctgcca tgttggattg tgcggctgcc gccgccgccg ctgccgccgc
       61 cgccgccaga ggactggagg agtagggagt ttagcgcagc cgccggagtg ctaggacaac
      121 gaccatccag ccggagctga gcagggcggg agcccggagc ttccctgtcg gcgcgcggcc
      181 cgagggcgcc cgccgtcatc tccttccctc ggcctccgcg cgccggagga gcgacgacgg
      241 gatgctgcgg ctgtgaccgc cggctcgcgc ccctgtcacc cagagtggga cccgagtgct
      301 cgcccgccgc cagaagtgac gaccctggac gctccacccc accggcgaca ccatgagctc
      361 gggccagcag cccccgcgga gggtcaccaa cgtgggctcc ctgctgctca ccccgcaaga
      421 gaacgagtct ctcttctcct tcctcggcaa gaaatgtgtg actatgtctt cagcagtggt
      481 gcagttgtat gcagcagatc ggaactgtat gtgggcaaag aagtgcagtg gtgtcgcttg
      541 tctggttaag gacaatcctc agagatctta ttttttaaga atatttgaca ttaaggatgg
      601 gaaattactg tgggaacaag agctatacaa taactttgta tataatagtc ctagaggata
      661 ttttcatacc tttgctggag atacttgtca agtagctctt aattttgcca atgaagaaga
      721 agcaaaaaag ttccgaaaag cagttacaga cctgttgggc cgacgacaaa ggaaatctga
      781 aaaaagacga gatgctccaa atggtcccaa tctacccatg gctacagttg acataaaaaa
      841 tccagaaatc acaacaaata gattttatgg ttcacaagtc aacaacatct cccacaccaa
      901 agaaaagaag aaaggaaaag ctaaaaagaa gagattaacc aaggcagata ttggaacacc
      961 aagtaatttc cagcacattg ggcatgttgg atgggatcca aatacaggtt ttgatctaaa
     1021 taatttggat ccagaattaa agaatctttt tgatatgtgt gggatctctg aggcccagct
     1081 taaagacaga gaaacatcaa aagttattta tgactttatt gaaaaaacag gaggtgtaga
     1141 agctgttaaa aacgaactcc gaagacaagc accaccacca cctccaccct cgagaggagg
     1201 acctccccct cctcctcccc ctcctcatag ctcaggtcct cctccccctc ctgcccgagg
     1261 aaggggggct cctcctcccc caccttcaag agctcctact gctgcacctc cacctccacc
     1321 tccttctagg cctggtgttg ttgttcctcc gccccctcca aacaggatgt accctcctcc
     1381 accaccagcg ctgccctctt cagcaccttc aggccctcca ccacctccac ctccatctat
     1441 ggcagggtcc acagcaccac cacctcctcc tccacctccg cccccaccag ggccaccacc
     1501 tccccctggc ctgccttctg atggtgacca tcaagttcca gctccttcag gaaacaaagc
     1561 agctcttttg gatcaaatta gagagggtgc tcagctgaaa aaagtggaac agaatagccg
     1621 gcccgtgtcc tgctcaggaa gggatgcgct tttagaccag atacgacagg gcattcaatt
     1681 gaaatctgtg tctgatggcc aagagtccac accaccaaca cccgcaccca cttcaggaat
     1741 tgtgggtgcg ctgatggaag tgatgcagaa aaggagcaaa gccattcatt cctcagatga
     1801 agatgaagac gatgatgatg aagaagattt tgaggatgat gatgagtggg aagactgatc
     1861 tatatattat atatatatat ttttaaggtg aaatactaaa cactacttcc tgtctattga
     1921 tctgtaaaat tatcatgtaa atgttctacc aattggctgt atatttttgt tgcctttttt
     1981 gctttttttt tttaaattaa tctgtgcaat acctcattta atctgtaaag gtttgttata
     2041 atcctatata aagctactcc ttgttgctgt gtgcaagttt acatcaggat gctcacgtga
     2101 tttgtgaata ttttcattcc atcaacaagg gagtttaatt atgtgtgaga ctgacacaaa
     2161 ccttaatgta atttagttag aatgccagaa ggagaacact attttcatac cctacttttt
     2221 ctgtacctaa attttcttaa taaaatttag tatagcacta cattcttttt taagtgatgt
     2281 aaactttagt ttctttagcc ccttcatttt gaatatgatg ccacatatga atgttgaagc
     2341 tgatacattg cacaattctc tagacaccac tcactacact aatgcagttt ttcaaattct
     2401 agtattatgt gttgcttaag atcactacag agatgctact gaagagagac ggtagtacag
     2461 ctctcttgca gtactgcctg ccgttggtaa gcagtggcat ccgaaggcag gaaccctgat
     2521 gagccctcac gtgcctgtcc ttgaccaagg tgacttttat gctgtggcaa ctgtgacagg
     2581 ctatttttta gcaaagagtt agacgtttgt tatcatgccc caaaatagtt tgaggtaaca
     2641 tttttggagg tagcttcgtg catcataaac aactttcgtg ctcgtgtctg actcgagcgg
     2701 tatcagaaca gggctgggat ggagtcttcg tgttttgcac cctgtgcatt gctatattca
     2761 caagtaccaa ctgtaggagt cagaggtgtt ggcaaaagta tgcatgaatg ctaacctttg
     2821 cacatactct gtttttactt tgatggcttc tccaggtttt tgtcaggtac cacatgtaga
     2881 attacatttt tcttaaaaga ttgatcatcc aaatggcaaa tttcttagag tccataactg
     2941 atttgataga tttttctttt taaataaaac ttcacagcct gcacagttgt tacgcatact
     3001 acatggtatg ttacaattag ggtttccttg cctctttaca gtacactaat ccgcctaaag
     3061 gtggctgttt gatatttacc gtgataatta ccttacctac ctacctactt tcaatacact
     3121 ttaggtagcc gattatctga tttaagtgca gacacacttt tctcacattg agttgtatgc
     3181 agaatgtagc ttcagatgta ttcattatag ttacaatatc caactaaaaa gtacgctatc
     3241 tagaagtata ccgaccaaaa tctagtattg acatgatctt gacaggctgg acctgtgaga
     3301 tatggtttga attttaacca gtttatcaca taacctctag agaccatgca tctagttatt
     3361 gtcaggaata atttaaaagg tttagtcgcc aaaagcagtt aagtggtgca gtaagatagt
     3421 taagttattc aaagagtgtt accctccttg caagagtaac tgaagcacat tggaaagatt
     3481 ctcaattttg tgtttcctgt aaaaacagtg ctctctgctg ctaggacctt ttttctgctt
     3541 cctgtcattt tcatgtggct tgagctctga gacgtgcgga tgaggctgga caactactac
     3601 ccatacaatt agggggtcaa aataacttta aataaatagc atcggacttt catatttttc
     3661 tttttgacgt ggagaaaaca ttttcatgcc taaaaatgtc tttgagataa tgccaattct
     3721 gatgtccctt accttttggc caagaagttt gagatttttt cagctaattt gttatttgac
     3781 cagaagttgt ctggggagac tgagttcttt gagttgcata taaatggggg tatattattt
     3841 gccaaaggta aaattgagtt tattcttttt catacagaaa tggattttgc ttttgtttgc
     3901 tttttatttt tgtgtaaaat ctaaattcca ggagctatgg ggttcatcca ggcatgaagg
     3961 ctaatccact ctgagtaaag tgtcagttgc acagctccgt cctcttctgt cttgcactgt
     4021 gggtggcact atttattcct gtgcgtcatc tggaagcctc acggatacat ccagctatgt
     4081 gcttgtctgg tagcctttgc gtttaacaaa ctagcatgag gcctttcata tctaaatgct
     4141 gtgtgataga caatttcagc tagccacata ttgtattatg aaattcataa agattttcaa
     4201 tcattcaatt tcatttatca actgaaattt tgtttagtat gtgaattttt ttttgaaatg
     4261 tatagtaaat aagatgttgc actggtggca attcatcatg gaacataata aaattaattt
     4321 gacccaaata gtaaaaaaaa aaaaaaaa
//
