LOCUS       NM_153548               4073 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus WASH complex subunit 5 (Washc5), mRNA.
ACCESSION   NM_153548
VERSION     NM_153548.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Lim,S.H., Shin,S., Lee,N.Y., Min,S.S., Kim,N.S., Lee,D.Y. and
            Lee,J.R.
  TITLE     Strumpellin/WASHC5 regulates the structural plasticity of cortical
            neurons involved in gait coordination
  JOURNAL   Biochem Biophys Res Commun 673, 169-174 (2023)
   PUBMED   37392480
  REMARK    GeneRIF: Strumpellin/WASHC5 regulates the structural plasticity of
            cortical neurons involved in gait coordination.
REFERENCE   3  (bases 1 to 4073)
  AUTHORS   Schurr,Y., Reil,L., Spindler,M., Nieswandt,B., Machesky,L.M. and
            Bender,M.
  TITLE     The WASH-complex subunit Strumpellin regulates integrin
            alphaIIbbeta3 trafficking in murine platelets
  JOURNAL   Sci Rep 13 (1), 9526 (2023)
   PUBMED   37308549
  REMARK    GeneRIF: The WASH-complex subunit Strumpellin regulates integrin
            alphaIIbbeta3 trafficking in murine platelets.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4073)
  AUTHORS   Clemen,C.S., Schmidt,A., Winter,L., Canneva,F., Wittig,I.,
            Becker,L., Coras,R., Berwanger,C., Hofmann,A., Eggers,B.,
            Marcus,K., Gailus-Durner,V., Fuchs,H., de Angelis,M.H., Kruger,M.,
            von Horsten,S., Eichinger,L. and Schroder,R.
  CONSRTM   German Mouse Clinic Consortium
  TITLE     N471D WASH complex subunit strumpellin knock-in mice display mild
            motor and cardiac abnormalities and BPTF and KLHL11 dysregulation
            in brain tissue
  JOURNAL   Neuropathol Appl Neurobiol 48 (1), e12750 (2022)
   PUBMED   34312900
  REMARK    GeneRIF: N471D WASH complex subunit strumpellin knock-in mice
            display mild motor and cardiac abnormalities and BPTF and KLHL11
            dysregulation in brain tissue.
REFERENCE   5  (bases 1 to 4073)
  AUTHORS   Song,L., Rijal,R., Karow,M., Stumpf,M., Hahn,O., Park,L.,
            Insall,R., Schroder,R., Hofmann,A., Clemen,C.S. and Eichinger,L.
  TITLE     Expression of N471D strumpellin leads to defects in the
            endolysosomal system
  JOURNAL   Dis Model Mech 11 (9) (2018)
   PUBMED   30061306
  REMARK    GeneRIF: These results underscore a key role for the WASH complex
            and its core subunit, Str, in the endolysosomal system.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4073)
  AUTHORS   Jahic,A., Khundadze,M., Jaenisch,N., Schule,R., Klimpe,S.,
            Klebe,S., Frahm,C., Kassubek,J., Stevanin,G., Schols,L., Brice,A.,
            Hubner,C.A. and Beetz,C.
  TITLE     The spectrum of KIAA0196 variants, and characterization of a murine
            knockout: implications for the mutational mechanism in hereditary
            spastic paraplegia type SPG8
  JOURNAL   Orphanet J Rare Dis 10, 147 (2015)
   PUBMED   26572744
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4073)
  AUTHORS   Wang,F., Zhang,L., Zhang,G.L., Wang,Z.B., Cui,X.S., Kim,N.H. and
            Sun,S.C.
  TITLE     WASH complex regulates Arp2/3 complex for actin-based polar body
            extrusion in mouse oocytes
  JOURNAL   Sci Rep 4, 5596 (2014)
   PUBMED   24998208
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4073)
  AUTHORS   Harbour,M.E., Breusegem,S.Y., Antrobus,R., Freeman,C., Reid,E. and
            Seaman,M.N.
  TITLE     The cargo-selective retromer complex is a recruiting hub for
            protein complexes that regulate endosomal tubule dynamics
  JOURNAL   J Cell Sci 123 (Pt 21), 3703-3717 (2010)
   PUBMED   20923837
REFERENCE   9  (bases 1 to 4073)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   10 (bases 1 to 4073)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113269.13 and BC067035.1.
            
            On Jul 12, 2018 this sequence version replaced NM_153548.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067035.1, AK128970.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC113269.13        181362-181396       c
            36-4073             BC067035.1         1-4038
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 25.17 cM"
     gene            1..4073
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /note="WASH complex subunit 5"
                     /db_xref="GeneID:223593"
                     /db_xref="MGI:MGI:2146110"
     exon            1..147
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            148..453
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..3747
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /note="WASH complex subunit strumpellin"
                     /codon_start=1
                     /product="WASH complex subunit 5"
                     /protein_id="NP_705776.2"
                     /db_xref="CCDS:CCDS37083.1"
                     /db_xref="GeneID:223593"
                     /db_xref="MGI:MGI:2146110"
                     /translation="MLDFLAENNLCGQAILRIVSCGNAIIAEVLRLSEFIPAVFLLKD
                     RADQQRYGDIIFDFSYFKGPEFWESKLEAKPELQDLDEEFRENNIEIVTRFYLAFQSV
                     HKYIVDLNRYLDDLNEGVYIQQTLETVLLSEDGKQLLCEALYLYGVMLLVIDQKIEGE
                     VRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPPNYPESYFQRVPIN
                     ETFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKM
                     REIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLANVKEQASRYASVS
                     DRVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRL
                     RQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERM
                     TELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEV
                     QEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTS
                     IMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVR
                     KVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISI
                     FTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPK
                     LKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQ
                     SMYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVT
                     SSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMS
                     AVNPLKSIVANSSKVYLSAITKTQKIWSAYLEAIMKVGQMQILRQQIANELNSSCRFD
                     SRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIY
                     ITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFH
                     SRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAH
                     VPNFIFDEFRTVL"
     exon            454..599
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            600..684
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            685..785
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            786..978
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1131
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1245
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1417
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1545
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1675
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1788
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1955
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2031
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2142
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2283
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2364
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2466
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2646
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2771
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..2934
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3037
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3117
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3118..3221
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..3351
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3448
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3602
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3603..3690
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     exon            3691..4053
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4033..4038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /note="hexamer: AGTAAA"
     polyA_site      4053
                     /gene="Washc5"
                     /gene_synonym="E430025E21Rik; strumpellin"
                     /note="major polyA site"
ORIGIN      
        1 ggtcacgtga cgggctaggg actccaggac agaaacccgg gtcccggcag ctgacgctct
       61 gcggcccggg actctagggg gatccctggg gtgggagacg gggaccgggg cgcccagtcc
      121 acgcggccag cgtcggacgg ctgacaggtt ctttaatgga gcggccattt gtcctacaga
      181 tctggcttca tgtcatgatc caccaacccc tgctctgaag tcagtcattc ccacccttca
      241 ggtgtggact agccagtcac tctcaccatg ttggacttcc tggctgagaa taatctctgt
      301 ggccaggcca tcctgaggat cgtctcctgt ggcaatgcca tcattgccga ggtgctgcgg
      361 ctctctgagt tcattcctgc tgtgttcctg ctgaaagacc gggctgacca gcagagatat
      421 ggagacatca tctttgactt cagctacttt aagggtccag agttctggga aagcaaactg
      481 gaagccaagc ctgagctgca ggatttagat gaagaatttc gggaaaacaa catagaaatc
      541 gtgaccagat tttatttagc ctttcaaagt gtgcataaat acattgtaga cttaaacaga
      601 tacctcgatg acctcaatga aggagtttat attcagcaaa ccttagaaac cgtgcttctc
      661 agtgaagatg gaaaacaact tctatgtgaa gccctgtact tatatggagt tatgctgctt
      721 gtcatcgacc agaagattga aggagaagtc agagagagaa tgctggtttc ttactatcga
      781 tacagtgctg ctcggtcctc tgctgactca aacatggatg atatctgtaa gctgctacga
      841 agcacaggat attccagcca gccaggagcc aaaagaccac ccaactatcc tgagagctat
      901 ttccagagag tgcctatcaa cgagacgttc atcagcatgg tcatcggacg cctgaggtct
      961 gatgacattt acaaccaggt ctctgcctac ccgctgccgg agcaccgcag cactgccctg
     1021 gccaatcaag ccgccatgct ctacgtgatt ctctattttg aaccttccat tcttcacacc
     1081 caccaagcaa aaatgagaga gatcgtggat aagtactttc cagataactg ggtaatcagc
     1141 atctatatgg gtatcactgt taatctagct gatgcctggg aaccttacaa agctgcgaaa
     1201 actgctctca acaacaccct tgacctcgcc aatgtcaaag aacaggcaag cagatacgct
     1261 agtgtcagcg acagagtgcg cgctcaggtg cagcagtttc tgaaagaggg ctacctgagg
     1321 gaggaggtgc ttctggacaa catccccagg ctgctgaact gcctgagaga ctgcaatgtc
     1381 gccatccgct ggctaatgct ccacacagcc gactcagcct gtgacccaaa caacaagcgc
     1441 cttcgtcaaa tcaaggacca gattctggca gactctaggt acaatcccaa gatcctcttc
     1501 cagttgctat tggacactgc acaattcgag tttatactca aagagatgtt caagcaaatg
     1561 ctttccgaga aacagtccaa gtgggagcat tacaagaagg agggctctga gcgcatgacc
     1621 gagcttgctg acgtcttttc aggggtgaaa ccactaaccc gagtggagaa aaacgaaaat
     1681 cttcaagctt ggttcagaga gatctcaaaa caaatactgt ctctaaatta tgatgattct
     1741 actgctgcag gcagaaaaac tgtgcaacta atacaagctc tggaagaggt tcaggagttt
     1801 caccagttgg aatcaaatct acaagtttgc cagtttctag ctgacacccg aaagtttctc
     1861 catcaaatga tcagaaccat caacatcaag gaggaagtcc tgatcacagt gcagatcatc
     1921 ggggaccttt cctttgcctg gcagttaatt gacagtttca catccatcat gcaagaaagc
     1981 ataagggtaa acccatccat ggttaccaaa ctcagagcca cgttcctaaa gcttgcctct
     2041 gcccttgacc ttcccctgct acgtattaat caagcaaata gccctgatct cctcagtgtg
     2101 tctcagtatt attcaggaga attggtgtca tacgtgagaa aggttttgca gatcattcca
     2161 gaaagcatgt tcacatctct cctgaagatc ataaagcttc agacccatga catcatggaa
     2221 gtgcctacac gcctggacaa ggacaagctg cgggactacg ctcagcttgg cccgagatat
     2281 gaggttgcca agctcactca cgccatctcc atctttactg agggcatctt gatgatgaaa
     2341 acgaccctgg tgggcatcat caaggtggat cccaagcagt tgctggaaga cggaataagg
     2401 aaagagctgg ttaaacgcgt ggctttcgcc cttcaccggg gactgatatt caaccctcgt
     2461 gccaagccaa gtgagttgat gcccaaactg aaagagctgg gggccaccat ggatgggttc
     2521 catcgctcct ttgagtatat acaggactac gtcagcattt acggtctgaa gatttggcag
     2581 gaggaagtct ctcgtattat aaattataac gtggaacagg agtgtaataa cttcttaaga
     2641 acaaagattc aagactggca gagtatgtac caatccactc atataccaat cccgaagttt
     2701 gcacctgtgg atgagtctat aacatttatt ggtcgactct gcagagaaat cttgcggatc
     2761 acagacccaa aaatgacatg ttacatagac cagctgaaca cttggtatga cgtgaaaact
     2821 catcaagaag tgaccagcag tcgcctcttc tcagaaatcc agaccacctt ggggactttt
     2881 ggtctgaatg gattggacag gcttctgtgc ttcatgattg tgaaagagct acagaatttc
     2941 ctcagtatgt ttcagaaaat tatcctgaaa gagagaactg ttcaagaaac tttaaagatg
     3001 ctcatgagtg ctgttaaccc tctaaagagc atagtggcaa attcaagtaa agtttatctt
     3061 tctgccatca ccaaaacaca gaagatctgg agtgcttacc tggaggctat aatgaaggtt
     3121 ggacagatgc agatcctgag acaacagatc gccaatgagc taaactcttc ctgtcgattt
     3181 gattccagac atctggcagc tgccctggat aatctcaata aggctctcct ggcagacatc
     3241 gaagcccact accgggaccc ttcgcttcct taccccaaag aagataacac actcttatat
     3301 gaaatcacag cctatctgga ggcggctggc attcacaacc ccctgaataa gatttacata
     3361 acaacgaagc gcttacccta tttcccgatt gtcaactttc tgtttttgat cgcgcagttg
     3421 ccaaaacttc agtataacaa aaatctaggc atggtctgcc ggaagcctgc cgaccctgtt
     3481 gactggccac cgctcgtcct aggtctgctc acgctgctga agcagttcca ctcccggtac
     3541 accgagcaat tcctggcact gattggccag tttattcgct ccacaatgga gcagtgcaca
     3601 agccagaaga tgcctgaaat gcctgcagat gctgtgggcg cccttctctt cctggaggat
     3661 tatgttcggt atacaaaact cccccgaagg gtggctgaag cccatgtgcc taacttcatt
     3721 tttgatgaat tccgaacagt cctgtgactt ctttctcctg ctccagtgac aggatgttct
     3781 atgactagaa tgaacttgga cgtgagaaag atacttgact cctcatgcaa cgtgcatttt
     3841 cctcctctgt gggaaccagc cattctaagg aaagtggctc tcaactgtct aaatcatggc
     3901 atcgtaagga atttttatca tgtagatgca gaactgtttt gtacttgcct cttttaaatg
     3961 ctcagtataa ctgatggatg aagccattcc atcttatgtt ctaaggatct gtttgtgcaa
     4021 ctccagacct tcagtaaaac agcattgcta gtaaaaaaaa aaaaaaaaaa aaa
//
