LOCUS       NM_001033375            5863 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus WASH complex subunit 4 (Washc4), mRNA.
ACCESSION   NM_001033375 XM_193573 XM_912191
VERSION     NM_001033375.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Courtland,J.L., Bradshaw,T.W., Waitt,G., Soderblom,E.J., Ho,T.,
            Rajab,A., Vancini,R., Kim,I.H. and Soderling,S.H.
  TITLE     Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and
            cognitive-movement impairments in mice and humans
  JOURNAL   Elife 10, e61590 (2021)
   PUBMED   33749590
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5863)
  AUTHORS   Tyrrell,B.J., Woodham,E.F., Spence,H.J., Strathdee,D., Insall,R.H.
            and Machesky,L.M.
  TITLE     Loss of strumpellin in the melanocytic lineage impairs the WASH
            Complex but does not affect coat colour
  JOURNAL   Pigment Cell Melanoma Res 29 (5), 559-571 (2016)
   PUBMED   27390154
REFERENCE   4  (bases 1 to 5863)
  AUTHORS   Harbour,M.E., Breusegem,S.Y., Antrobus,R., Freeman,C., Reid,E. and
            Seaman,M.N.
  TITLE     The cargo-selective retromer complex is a recruiting hub for
            protein complexes that regulate endosomal tubule dynamics
  JOURNAL   J Cell Sci 123 (Pt 21), 3703-3717 (2010)
   PUBMED   20923837
REFERENCE   5  (bases 1 to 5863)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   6  (bases 1 to 5863)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
REFERENCE   7  (bases 1 to 5863)
  AUTHORS   Hughes,D.S., Keynes,R.J. and Tannahill,D.
  TITLE     Extensive molecular differences between anterior- and
            posterior-half-sclerotomes underlie somite polarity and spinal
            nerve segmentation
  JOURNAL   BMC Dev Biol 9, 30 (2009)
   PUBMED   19463158
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5863)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 5863)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5863)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ561687.1, AK038582.1, AK161247.1 and AI605037.1.
            
            On Feb 23, 2007 this sequence version replaced NM_001033375.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849381,
                              SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                BQ561687.1         1-43
            44-3534             AK038582.1         2-3492
            3535-5854           AK161247.1         983-3302
            5855-5863           AI605037.1         1-9                 c
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 41.29 cM"
     gene            1..5863
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="WASH complex subunit 4"
                     /db_xref="GeneID:319277"
                     /db_xref="MGI:MGI:2441787"
     exon            1..162
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..3623
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="WASH complex subunit 7; WASH complex subunit SWIP"
                     /codon_start=1
                     /product="WASH complex subunit 4"
                     /protein_id="NP_001028547.2"
                     /db_xref="CCDS:CCDS36015.1"
                     /db_xref="GeneID:319277"
                     /db_xref="MGI:MGI:2441787"
                     /translation="MAVDTLSPDWDFDRVDDGSQKIHAEVQLKNYGRFLEEYTSQLRR
                     IEDALDDLIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIK
                     KLKYEAETKFYNGLLFYGEGATDSSMVEGDCQIQMGRFVSFLQELSCFVTRCYEVVMN
                     VIHQLAALYISNKIGPKIIETTGVHFQTMYEHLGELLTVLLTLDEIVDNHVTLKDHWT
                     MYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGI
                     SVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTVDK
                     KFYKSLLDICKKVPAITLTANIIWFPDNFLIHKMPAAAKLLDRKSLQAIKIHRDTFLQ
                     QKAQSLNKDVQSYYVFVSSWMMKMESMLSKEQRMDTFAEDLTNRCNVFIQGFLYAYSI
                     STIIKTTMNLYMSMQKPMTKTSVKALCRLIELLKAIEHMFYRRSMVVADSVSHITQHL
                     QHQALSSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVG
                     TQMKTFKDEELFPLQVVMKKLDLISELRERVQAQCDCCFLYWHRAVFPIYLDDVYENA
                     VDAARLHYMFSALRDCVPAMMHSRHLESHELLLDCYDKEIMDILNEHLLDKLCKEIEK
                     DLRLSVHTHLKLDDRNPFKVGRKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNL
                     TTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLY
                     NLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQF
                     MYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGITPEGQSYLDQFRQ
                     LISQIGNAMGYIRMIRSGGLHCSSNAIRFVPDLEDIVSFEELVKEEGLAEETLRAARH
                     LDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSIS
                     CKEKLNKKNKLGAAFTDDGFAMGVAYTLKLLDQYQEFDSLHWFQSVREKYIKEIRAVA
                     KQQNVQSTSQDEKLLQTMNLTQKRLEVYLQEFELLYFSLSSARIFFRADKTAAEENQE
                     KKEKEEETKTSNGDGPESTVSADPVVK"
     exon            163..302
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            303..356
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            357..422
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            423..468
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            469..536
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            537..619
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            620..662
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            663..766
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            767..887
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1011
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1139
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1300
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1427
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1553
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1661
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1808
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1888
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..1994
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2111
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2280
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2435
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2510
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2615
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2750
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2859
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..2926
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3013
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            3014..3161
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            3162..3258
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3455
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            3456..3555
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     exon            3556..5858
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5750..5755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="hexamer: ATTAAA"
     polyA_site      5769
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="major polyA site"
     regulatory      5837..5842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="hexamer: AATAAA"
     polyA_site      5858
                     /gene="Washc4"
                     /gene_synonym="A230046K03Rik; Kiaa1033; mKIAA1033"
                     /note="major polyA site"
ORIGIN      
        1 ggggccgaag ccggaagtca cgtgccgtga cagcggctgg ggtgaggccc gcgtcgcggc
       61 tgtggctgct cggggctgtg tctgtgggag gttccgcggc gatggccgtg gacactctgt
      121 cgcccgactg ggacttcgac cgcgtggacg acggctctca gaaaattcat gctgaagtcc
      181 agctgaagaa ctacgggagg ttccttgagg agtacacatc tcagctgaga agaattgagg
      241 acgccctaga tgatttgatt ggcgatgttt gggatttcaa tcttgatcct atagcattaa
      301 agcttctgcc ctatgagcag tcttcccttt tggaactcat aaagactgaa aacaaggtcc
      361 tgaacaaagt catcactgtt tatgcggcac tttgttgcga gatcaagaaa ttaaaatatg
      421 aggcagaaac taagttttac aatggtcttc tgttttatgg tgaaggagct acagattcca
      481 gcatggtgga aggtgactgc caaattcaaa tggggagatt cgtttcattc ttacaggaac
      541 tgtcctgctt tgtgacgagg tgctacgaag tggtgatgaa tgtcatccac cagctggctg
      601 ccctgtacat cagcaacaag ataggaccca aaattataga gacaactgga gttcattttc
      661 agactatgta tgagcacttg ggagaactgc taacagtttt acttaccctg gatgagattg
      721 ttgataatca tgtcacgctg aaagaccact ggactatgta caaaaggttg ctgaaatctg
      781 tccatcacaa cccttcaaaa tttgggattc aggaagaaaa gttaaagccc tttgagaagt
      841 tcttgctgaa gctggaaggg cagttacttg atggaatgat atttcaggcc tgtatcgaac
      901 agcagtttga ttctctcaat ggaggaatat ctgtgtcgaa aaatagcacg tttgcagaag
      961 agtttgcaca tagcattcgc tcaatttttg caaatgtgga agccaagctt ggtgagcctt
     1021 ctgaaattga ccagagagac aagtatgttg gaatttgtgg gctctttgta ctgcactttc
     1081 agatttttcg gactgttgat aaaaagtttt ataagtcttt attggacatt tgtaagaagg
     1141 tgccagccat cactttaact gctaatatta tttggtttcc tgataatttt ctgattcata
     1201 aaatgccagc agctgccaaa cttctagaca gaaaaagtct tcaagccatt aaaatacaca
     1261 gggatacctt cctacagcag aaagctcagt cacttaacaa agatgtgcag tcttactacg
     1321 tctttgtgag ctcatggatg atgaagatgg agtctatgtt atctaaagag cagaggatgg
     1381 atacatttgc tgaagacctc accaacagat gcaatgtttt catacagggt ttcttatatg
     1441 catatagtat tagtaccatt attaagacca caatgaatct ctacatgtcc atgcagaagc
     1501 ctatgaccaa aacttcagtg aaggcattat gcaggctcat tgaacttctc aaggcaatag
     1561 agcatatgtt ctacaggaga agcatggttg tggcggattc ggtgtcacat ataacacagc
     1621 accttcaaca tcaggctctt agttctattt ctgtagccaa gaaaagagta atttcagaca
     1681 aaaaatatag tgaacagcgt cttgatgtgc tatctgctct tgttttggct gaaaacacct
     1741 taaatggacc aagcacaaaa caacgacgac ttattgtttc tttggcattg agcgttggca
     1801 cgcaaatgaa aacatttaaa gatgaagaac tctttcccct tcaagtagtc atgaagaagc
     1861 tggatcttat cagcgagctt agagagcgag tccaagcaca gtgtgactgc tgttttttat
     1921 actggcatcg cgctgtcttc ccgatttatt tagatgatgt atatgaaaat gctgtcgatg
     1981 cagccagatt acattacatg ttcagtgctt tacgagactg tgtgcctgcc atgatgcatt
     2041 caaggcattt agagtcccat gagctgcttt tggattgcta tgacaaggaa atcatggaca
     2101 ttttaaatga gcatttgctg gataagctgt gtaaggagat cgagaaggac ttgcggcttt
     2161 ctgtgcatac tcacttgaag ctggatgacc ggaacccctt caaagttggc aggaaagact
     2221 tggcgctctt tttctctctg aaccctattc ggtttttcaa tcgattcatt gatattcgag
     2281 cttatgtaac tcactatctg gataagactt tctacaatct cacaacagta gctcttcatg
     2341 attgggccac ttacagtgaa atgagaaatc tggctacgca gcgttatggt ttggttatga
     2401 cagaggcaca tcttcctagc cagactttgg agcagggact cgatgtcctg gaaattatga
     2461 gaaacattca catatttgtc tcaagatacc tctataatct caataaccag atttttattg
     2521 aacggacaag caacaataaa catttgaata ctattaatat tcgacacatt gctaattcga
     2581 ttcgaacaca tgggacgggg atcatgaata caacagttaa tttcacgtac cagtttttga
     2641 aaaagaagtt ctatatattt agtcagttta tgtatgatga gcatatcaaa tccagattga
     2701 ttaaagatat tcggtttttc agggagatca aggaccaaaa tgaccacaag tatccttttg
     2761 atagagcaga aaaattcaat cgtggcataa gaaaacttgg aatcacacca gagggacaga
     2821 gctaccttga ccagttcagg cagctcatca gccagattgg taatgctatg ggctatatac
     2881 gcatgataag atctggtggt cttcattgta gcagcaatgc cattagattc gtgccggatc
     2941 ttgaagacat tgtgagtttt gaagagctcg tgaaagaaga agggcttgca gaagaaacac
     3001 ttagagcagc caggcatttg gactcagtcc tcagtgatca tacacggaac tctgctgaag
     3061 gtacagaata tttcaaaatg cttgtagatg tttttgctcc agaatttcga aggccaaaga
     3121 atatacatct ccgaaatttc tatatcattg ttcctcctct tactctgaac tttgtagaac
     3181 attctataag ttgcaaagag aagttgaata aaaaaaataa actaggagct gcatttactg
     3241 acgatggttt tgccatgggt gtggcttata ccttaaagct tttggatcag taccaagagt
     3301 ttgattcact tcattggttc cagtctgtta gagaaaaata cataaaggag atcagagcag
     3361 ttgctaagca acagaacgtg cagtcaacta gtcaagatga aaaactactc caaaccatga
     3421 accttactca gaagcgactg gaggtctatt tacaggaatt tgagctgctg tatttctcct
     3481 tgagcagtgc aagaatattc ttcagagcag acaagaccgc agctgaagag aaccaagaaa
     3541 agaaagagaa ggaagaagaa actaaaacaa gcaatggaga tggacccgag agcactgtgt
     3601 ctgcagatcc cgtggtgaaa tgatggtgtg cagtcgtcag acgtatcatg atgcttttcc
     3661 agagacatgg atgctatggc gtgcttgata agttgtcatt tcccaggtca cacctaagga
     3721 gactgtacgg actgctctgg ctctggagtc aagtccactt tatttctgat gctccagctt
     3781 tcaagggccg agaagcaaac ggtaagactg gatctttgga gtgtaggctg aaggcgcccc
     3841 tgtgaaactg ccatgaagct ggcgaagggc aagtccagat ccctaaccca tcgcctccct
     3901 tccctgtact ctgtgtgtgt ttgtaatttg tctgtgtctt tgattcatgt tgatctcaag
     3961 tcatgggaaa gtcagtgcat atgcggtaca cagccagtaa ataacgcagt taaaaagagt
     4021 tgagcctgaa agtcatgaag catctgtatc agagactgtg tgaggatgct agacatgagc
     4081 agactgttac tgcctgtgca cagcgtggcc agctggtgat ccttcctgtg ttgttggatt
     4141 ccacgtgact cacatgagct gctgatgtct ttggtgagac attttataat agtttacatt
     4201 gctttgggaa catttatcct ccaacaattg ctttaaattt aagatttaat actcaaagaa
     4261 aactcagatt aagccataga cgggacccac ttgacatttc actaggtttg aaggcactct
     4321 gtgatactag ggaagaaaag aatgaactat ctacagccaa actcaggttt cttcattact
     4381 ttaaattgaa atcttggtgg cagtttagac agaattttac tttaaatcaa gtataatcta
     4441 agatataaga ttaaataata actgcactat gacaggtgtt cagaaatata ccactgtgag
     4501 aatgaaacta gttaagtcac ttactgattt taaaagaatg aaagactttg agcaaattta
     4561 gttcccaaca agctactagc tttatttttg taaaggtatg ctagctgata gttaaatgga
     4621 ttaatcatgg ccttttttga acgtatacta aaatgatgcc tttgggatgg aaaggaaggt
     4681 ttagaacttt aaatataata cagacctttt ttgggaattg cttgtttttt attgcattta
     4741 cctgcttaac catttctttg gatacatctt gcatgtaatc ctaacactta gaattctgac
     4801 attcttcctt ggttcccgct tttggagtgg agatacagtg cccaacctag actcagtggt
     4861 tgcggtcagg gcaactgtct cagcccaagc taaatacttt gactgttaag tcagaagaaa
     4921 cagaatagat tggaaatggt ttttattata taaacatggc ttatagaatt atgaacaaag
     4981 aatggattaa aggcatttaa tgtttgtaat tgatactaac tgtagagatg gccctaaagc
     5041 atgctgcata attaataatt tatattttca ttattataaa tgtttatatt aatgatactg
     5101 gatttgatta tatcaaatta gtcaccattt tatagtacca atatttctgt ctttttctta
     5161 aaaatgttct ttaattattc ctgaacatga gagttcacat attccttgga cacttaaaat
     5221 gggaagtgac aggtaactgt tttaacttta gttaaaaata atgtcactgg ggctggtgag
     5281 atggctcagt gggtaagagc acccgactgc tcttccgaag gtccagagtt caaatcccag
     5341 caaccacatg gtggctcaca accatctgta acaagatctg acgccctctt ctggagtgtc
     5401 tgaagacagc tacagtgtac ttacatataa taaataaata aatctttaaa aaaaaaaaaa
     5461 ataatgtcac tgtagtctgg ataaattata ataagttcat gtatttgaaa taaagtctct
     5521 gatgagagaa atatttaaat ggtattaaac tgctgagcaa cagggcttca gtatgttcac
     5581 acctgggtta ctgtatacgt tggatgatgg ttttcttttg ctaacaatat tttttcagaa
     5641 atattgtaat ttaaatatta acatgagtgt tttgttgtaa attcacagaa aacttaatga
     5701 tgttcaaatt ctcaggaatt aagaactaac cataacattg tcagttctga ttaaattttg
     5761 taaaagacag caagtttgtt atctcacttg agtggggttc tcctttccct tggctcaaat
     5821 agaatacaat acgtataata aagcattaat aaatataaaa aaa
//
