LOCUS       NM_026070               1048 bp    mRNA    linear   ROD 05-FEB-2024
DEFINITION  Mus musculus WASH complex subunit 3 (Washc3), transcript variant 1,
            mRNA.
ACCESSION   NM_026070
VERSION     NM_026070.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Tyrrell,B.J., Woodham,E.F., Spence,H.J., Strathdee,D., Insall,R.H.
            and Machesky,L.M.
  TITLE     Loss of strumpellin in the melanocytic lineage impairs the WASH
            Complex but does not affect coat colour
  JOURNAL   Pigment Cell Melanoma Res 29 (5), 559-571 (2016)
   PUBMED   27390154
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 1048)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   4  (bases 1 to 1048)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
REFERENCE   5  (bases 1 to 1048)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK158822.1 and AK013835.1.
            
            [WARNING] On Apr 23, 2024 this sequence was replaced by
            NM_026070.4.
            
            On Apr 2, 2008 this sequence version replaced NM_026070.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK013835.1, AK158822.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-897               AK158822.1         2-898
            898-1048            AK013835.1         903-1053
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 43.7 cM"
     gene            1..1048
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /note="WASH complex subunit 3"
                     /db_xref="GeneID:67282"
                     /db_xref="MGI:MGI:1914532"
     exon            1..196
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..730
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /note="isoform 1 is encoded by transcript variant 1; WASH
                     complex subunit CCDC53; coiled-coil domain containing 53;
                     coiled-coil domain-containing protein 53"
                     /codon_start=1
                     /product="WASH complex subunit 3 isoform 1"
                     /protein_id="NP_080346.1"
                     /db_xref="CCDS:CCDS24108.1"
                     /db_xref="GeneID:67282"
                     /db_xref="MGI:MGI:1914532"
                     /translation="MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRF
                     SAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSET
                     TSEQTQQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVGVPVMAIRDKMISEG
                     LDPELLEKPDAPVPNGESERAVEESSDSDSSFSD"
     exon            197..295
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     exon            296..361
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     exon            362..469
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     exon            470..580
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     exon            581..645
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     exon            646..1048
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      876..881
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /note="putative"
     polyA_site      897
                     /gene="Washc3"
                     /gene_synonym="2900091E11Rik; 5730495F03Rik; Ccdc53"
                     /note="putative"
ORIGIN      
        1 ctctgaagcc acccattgaa tggtgaacac tcgcgctttc ttccggaaag gccccgaagg
       61 caggaacttg ggaaaggagt ctgggctctc tactgggctg ggaggagctt gtgcctgagc
      121 cccggggtcc ccatccgctg aggagatgga tgaggatggg cttcctctca tggggtcagg
      181 catagacctg accaaggtgc cagctattca acagaaaaga accgtggcct ttctaaacca
      241 gtttgtggtg cacactgtgc agtttctcaa ccggttttcc gcagtttgtg aggagaaact
      301 ggcagacctt tctctccgga ttcagcaaat tgaaacaact ctcaatattt tagatgcaaa
      361 gctgtcatct atcccaggcc tagaggacgt cacagttgaa gtgtctcctt taaatgtcac
      421 tgctgtcaca aacggatcac attctgaaac cacttcggag caaacgcagc agaatagcac
      481 gcaagactct ggagcacagg agagtgaagc gccatcagaa aatgtcctga ccgtagccaa
      541 ggatccacgg tatgccagat acctcaagat ggttcaagtg ggtgttccag tgatggcaat
      601 cagagacaag atgatctcag aaggactgga cccggagcta ctggagaagc cagatgcccc
      661 ggtgccaaat ggcgaaagcg aaagagccgt agaagaaagt tcagatagtg actcttcctt
      721 cagtgactaa gggactgggg gaatcacaca gaacgtggaa gaatgtattt acgttctaga
      781 aaagagccta cgctctatct agctctccgt gataaacatc accaaatgct actgcatacc
      841 tccccaacct ttctctgttt aaaaataatt ataataataa agcatttata atctttcaat
      901 atggtttgtt gaccaaataa aatgctctga agttaacaga acaacataaa ttgctacaag
      961 cactgtaaat agtaagatgg gttcaatgaa cattctaagc tggagttagt ttgggtttac
     1021 cgatcaaata tagacttcat gtaaaaat
//
