LOCUS       NM_026833               2904 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus WASH complex subunit 1 (Washc1), transcript variant 1,
            mRNA.
ACCESSION   NM_026833 XM_128709 XM_915458
VERSION     NM_026833.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Heida,A., van Dijk,T., Smit,M., Koehorst,M., Koster,M.,
            Kloosterhuis,N., Havinga,R., Bloks,V.W., Wolters,J.C., de Bruin,A.,
            Kuivenhoven,J.A., de Boer,J.F., Kuipers,F. and van de Sluis,B.
  TITLE     Changes in bile acid composition are correlated with reduced
            intestinal cholesterol uptake in intestine-specific WASH-deficient
            mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1869 (2), 159445 (2024)
   PUBMED   38086439
  REMARK    GeneRIF: Changes in bile acid composition are correlated with
            reduced intestinal cholesterol uptake in intestine-specific
            WASH-deficient mice.
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Chen,H., Victor,A.K., Klein,J., Tacer,K.F., Tai,D.J., de Esch,C.,
            Nuttle,A., Temirov,J., Burnett,L.C., Rosenbaum,M., Zhang,Y.,
            Ding,L., Moresco,J.J., Diedrich,J.K., Yates,J.R. 3rd, Tillman,H.S.,
            Leibel,R.L., Talkowski,M.E., Billadeau,D.D., Reiter,L.T. and
            Potts,P.R.
  TITLE     Loss of MAGEL2 in Prader-Willi syndrome leads to decreased
            secretory granule and neuropeptide production
  JOURNAL   JCI Insight 5 (17), 138576 (2020)
   PUBMED   32879135
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2904)
  AUTHORS   Walpole,G.F.W., Plumb,J.D., Chung,D., Tang,B., Boulay,B.,
            Osborne,D.G., Piotrowski,J.T., Catz,S.D., Billadeau,D.D.,
            Grinstein,S. and Jaumouille,V.
  TITLE     Inactivation of Rho GTPases by Burkholderia cenocepacia Induces a
            WASH-Mediated Actin Polymerization that Delays Phagosome Maturation
  JOURNAL   Cell Rep 31 (9), 107721 (2020)
   PUBMED   32492429
  REMARK    GeneRIF: Inactivation of Rho GTPases by Burkholderia cenocepacia
            Induces a WASH-Mediated Actin Polymerization that Delays Phagosome
            Maturation.
REFERENCE   4  (bases 1 to 2904)
  AUTHORS   Wijers,M., Zanoni,P., Liv,N., Vos,D.Y., Jackstein,M.Y., Smit,M.,
            Wilbrink,S., Wolters,J.C., van der Veen,Y.T., Huijkman,N.,
            Dekker,D., Kloosterhuis,N., van Dijk,T.H., Billadeau,D.D.,
            Kuipers,F., Klumperman,J., von Eckardstein,A., Kuivenhoven,J.A. and
            van de Sluis,B.
  TITLE     The hepatic WASH complex is required for efficient plasma LDL and
            HDL cholesterol clearance
  JOURNAL   JCI Insight 4 (11), 126462 (2019)
   PUBMED   31167970
  REMARK    GeneRIF: The hepatic WASH complex is required for efficient plasma
            LDL and HDL cholesterol clearance.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2904)
  AUTHORS   Ding,L., Han,L., Dube,J. and Billadeau,D.D.
  TITLE     WASH Regulates Glucose Homeostasis by Facilitating Glut2 Receptor
            Recycling in Pancreatic beta-Cells
  JOURNAL   Diabetes 68 (2), 377-386 (2019)
   PUBMED   30425062
  REMARK    Erratum:[Diabetes. 2020 May;69(5):1083. doi: 10.2337/db20-er05a..
            PMID: 32152205]
REFERENCE   6  (bases 1 to 2904)
  AUTHORS   Monfregola,J., Napolitano,G., D'Urso,M., Lappalainen,P. and
            Ursini,M.V.
  TITLE     Functional characterization of Wiskott-Aldrich syndrome protein and
            scar homolog (WASH), a bi-modular nucleation-promoting factor able
            to interact with biogenesis of lysosome-related organelle subunit 2
            (BLOS2) and gamma-tubulin
  JOURNAL   J Biol Chem 285 (22), 16951-16957 (2010)
   PUBMED   20308062
  REMARK    GeneRIF: WASH is a bimodular protein and a component of the BLOC-1
            complex in which the C terminus is involved in Arp2/3-mediated
            actin nucleation, whereas the N-terminal portion is required for
            its regulation and localization in the cells
REFERENCE   7  (bases 1 to 2904)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
  REMARK    GeneRIF: show that WASH generates an actin network on a restricted
            domain of sorting and recycling endosomes.
REFERENCE   8  (bases 1 to 2904)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
REFERENCE   9  (bases 1 to 2904)
  AUTHORS   Li,H., Bishop,K.M. and O'Leary,D.D.
  TITLE     Potential target genes of EMX2 include Odz/Ten-M and other gene
            families with implications for cortical patterning
  JOURNAL   Mol Cell Neurosci 33 (2), 136-149 (2006)
   PUBMED   16919471
REFERENCE   10 (bases 1 to 2904)
  AUTHORS   Gianfrancesco,F., Falco,G., Esposito,T., Rocchi,M. and D'Urso,M.
  TITLE     Characterization of the murine orthologue of a novel human
            subtelomeric multigene family
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 98-100 (2001)
   PUBMED   11701968
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY275336.1, AK159820.1 and AC119957.9.
            
            On or before Dec 16, 2005 this sequence version replaced
            XM_128709.6, XM_915458.1.
            
            Transcript Variant: This variant (1) and variant 2 encode the same
            protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK171737.1, BC025839.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-438               BY275336.1         1-438
            439-1829            AK159820.1         367-1757
            1830-2904           AC119957.9         22878-23952         c
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 35.26 cM"
     gene            1..2904
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /note="WASH complex subunit 1"
                     /db_xref="GeneID:68767"
                     /db_xref="MGI:MGI:1916017"
     exon            1..104
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            105..258
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1537
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /note="WAS protein family homolog 1"
                     /codon_start=1
                     /product="WASH complex subunit 1"
                     /protein_id="NP_081109.1"
                     /db_xref="CCDS:CCDS28944.1"
                     /db_xref="GeneID:68767"
                     /db_xref="MGI:MGI:1916017"
                     /translation="MTPVKTQCSLAGQLYAVPLIQPDLRREEAIQQVADALQYLQNIS
                     GDIFSRISRRVELSRRQLQAISERVSLAQAKIEKIKGSKKAIKVFSSAKYPAPEHLQE
                     YGSIFTGALDPGLQRRPRYRIQSKHRPLDERALQEKLKYFPVCVNTKSEPEDEAEEGL
                     GGLPSNISSISSLLLFNTTENLYKKYVFLDPLAGAVTKTHTMLGTEEEKLFDAPLSIS
                     KREQLERQAPENYFYVPDLGQVPEIDVPSYLPDLPGVADDLMYSADLGPGIAPSAPGA
                     IPELPAFHTEVAEPLQPELENEVLLAAPPPPPPPPPPPPPAPTALVSTPQPPMFPDMA
                     TAAGQVAREEDSSSSMAHTASVQGAPKEVVDPSSGRATLLESIRQAGGIGKAKLRSVK
                     ERKLEKKKQKEQEQVRATSQGGDLMSDLFNKLVMRRKGISGKGPSTGTSEGPGGAFSR
                     MSDSIPPLPPPQQPAGDEDEEDWES"
     exon            259..370
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            371..517
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..654
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            655..787
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            788..989
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1169
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1328
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1397
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..2904
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1812..1817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
                     /note="hexamer: AATAAA"
     polyA_site      1849
                     /gene="Washc1"
                     /gene_synonym="1110049F14Rik; ORF19; Wash; Wash1"
ORIGIN      
        1 gagagccgga agtcggagag ccgagcttac agtggtggac gctaggacct gggagtgcgg
       61 gtcaccgggg ggcggggacg ctcacgtgag gacgccgcct tggggcatca tgacaccggt
      121 gaagacccag tgctccttgg cgggccagct ttatgcagtg cccctcatcc agcccgacct
      181 gcgacgagag gaggcaatcc agcaagtggc agatgccctg cagtacctac agaacatctc
      241 tggagacatc ttcagcagga tctcccgacg agtagagctg agtcggcgcc agctgcaggc
      301 cattagtgag agggtctcct tggcccaggc taagatagag aagatcaagg gcagtaagaa
      361 ggccatcaag gtcttttcta gtgccaagta cccagcacca gagcacctgc aggaatacgg
      421 ctccatcttt actggcgcct tggaccctgg tctgcagaga agaccccgtt acaggatcca
      481 gagcaagcac cgcccactgg atgagcgggc tctgcaggag aagctgaaat actttcccgt
      541 gtgtgtgaac accaagtcgg agcctgagga tgaagctgag gagggacttg ggggtcttcc
      601 cagcaacatc agctccatca gctccctgct gctcttcaac accacagaga atctgtataa
      661 gaagtatgtt ttcctggacc ctctggctgg tgcagtaaca aaaacgcaca cgatgctggg
      721 cacagaggag gagaaattgt tcgatgcccc tctgtctatc agcaagagag agcagctgga
      781 gcggcaggct ccagaaaact acttctacgt gccagacctg ggccaggttc ctgagatcga
      841 cgtgccatcc tacctacccg atttacccgg cgtcgcagac gacctgatgt acagtgcgga
      901 tttgggccct ggcattgccc cgtctgctcc tggtgctatc ccagagttgc ctgccttcca
      961 cacagaggtg gcagaacctc tccagccaga gctagagaat gaagtactgt tggcagcacc
     1021 gcccccgcct cctcccccac cgccgccacc tcccccagct cccacagcat tggtcagtac
     1081 cccacagcca ccgatgttcc ctgacatggc aactgctgct ggccaggttg ccagggaaga
     1141 agactctagc agcagcatgg cccacacagc ctctgttcag ggagccccta aggaggtggt
     1201 tgacccctcc agtggccggg ccactctgct cgagtccatc cgccaagctg ggggcattgg
     1261 caaggccaag ctacgcagcg tgaaggagcg caaactggag aagaagaaac agaaggagca
     1321 ggagcaagtg agagccacga gccaaggtgg ggacttgatg tcagatctct tcaacaaact
     1381 tgtcatgagg cgcaaaggta tctctggaaa gggacctagc actggaacca gtgagggacc
     1441 tggaggagcc ttctctcgaa tgtcagactc catcccaccg ctgccacccc cacagcagcc
     1501 ggcaggagac gaggatgagg aggactggga gtcctaagag tgctttggcc attctcccac
     1561 atggcagagc agctggcagg ggctcctcgg ccagttgggg cctttcaggg cctgttgccc
     1621 aaggaggtgc agggggctgg gcaggtgtcc caggcctgtg cctaattcct cttcatggag
     1681 tcgggactgt atgagccatc tgctggcttt gctgaagagc ttcttccttc aaaggatcct
     1741 gagagaaaac agcaacacgg cggaataaag aaagtaactg tagacctgaa cccaagccaa
     1801 cagtggcctt taataaatga agagtagcca tgcgggctgc ggcttctgag agtgggagct
     1861 gtcctttttc ccaggtgtgc tgaggcatgg tgcaggatga ctgggggaat ggacgtcttt
     1921 ctctgggatc aaggcaaatg gggatgaggt ccgcagggga cagagggagg ggtagctttc
     1981 cactggaaaa atgccttgct gtttgctgag gtggctgagg acaggaggga ctgcaagcca
     2041 cagggagggc ttggaggcaa accggggagg gggacctgtg aaggactcag gagggacaga
     2101 actgcagtta ggaactgaaa ctggtgtgag gtccctgtga acacagccct gaaaatgtgg
     2161 gtctctgcgg tttagatgtt gcctccacag aggcctcctg tgatggacca ggctctgtcc
     2221 tcagccaagc ctggtctcca gcctgaagtc cagcaggaca ttgatccagg ccttgttgag
     2281 ccgggcgggc ggatctcagg ctgaactgcc ctgggtagtt gcagtttcct caagataatc
     2341 gagatttgct cctggtgtct ctttgggata tgggccactt ggggaccact cgggggtgcc
     2401 acagggtgtc agaagacctt ctctctgggt gaaactgcac attcaggaac gtttaccaag
     2461 ggtttgttgg gagggaaagg cagactgaac cttgtacact cagagaaggc aagaccaagg
     2521 atgctctccc tgcactgggt gtggaaacca gaatggcagg tgtactggct gattttgtgt
     2581 gtctacttga catggctgga gttatcacag agaaacgagc ttcagttggg gaagtgtctc
     2641 cgtgagatcc agctgtaagg cattttctca attagtgatc aaggaggaaa ggccccttgt
     2701 gggtggtacc atctctgggc tggtggtcct gggttctata agaaagcaag ctgaggaagc
     2761 cagtggaagc aagccagtaa ggaacatccc tccatggcct ctgcatcagc tcctgcttcc
     2821 tgacctgctt gagttccagt cctgcatcct ttggtgatca acagcaatgt ggaaagtgta
     2881 aactgaataa accctttcct cccc
//
