LOCUS       NM_001418239            4965 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus WASP family, member 3 (Wasf3), transcript variant 2,
            mRNA.
ACCESSION   NM_001418239
VERSION     NM_001418239.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Zhang,Y., Kang,H.R., Jun,Y., Kang,H., Bang,G., Ma,R., Ju,S.,
            Yoon,D.E., Kim,Y., Kim,K., Kim,J.Y. and Han,K.
  TITLE     Neurodevelopmental disorder-associated CYFIP2 regulates
            membraneless organelles and eIF2alpha phosphorylation via protein
            interactors and actin cytoskeleton
  JOURNAL   Hum Mol Genet 33 (19), 1671-1687 (2024)
   PUBMED   38981622
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Kansakar,U., Wang,W., Markovic,V. and Sossey-Alaoui,K.
  TITLE     Phosphorylation of the proline-rich domain of WAVE3 drives its
            oncogenic activity in breast cancer
  JOURNAL   Sci Rep 11 (1), 3868 (2021)
   PUBMED   33594155
  REMARK    GeneRIF: Phosphorylation of the proline-rich domain of WAVE3 drives
            its oncogenic activity in breast cancer.
            Erratum:[Sci Rep. 2021 Jul 29;11(1):15800. doi:
            10.1038/s41598-021-94981-0.. PMID: 34326443]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4965)
  AUTHORS   Corral-Serrano,J.C., Lamers,I.J.C., van Reeuwijk,J., Duijkers,L.,
            Hoogendoorn,A.D.M., Yildirim,A., Argyrou,N., Ruigrok,R.A.A.,
            Letteboer,S.J.F., Butcher,R., van Essen,M.D., Sakami,S., van
            Beersum,S.E.C., Palczewski,K., Cheetham,M.E., Liu,Q., Boldt,K.,
            Wolfrum,U., Ueffing,M., Garanto,A., Roepman,R. and Collin,R.W.J.
  TITLE     PCARE and WASF3 regulate ciliary F-actin assembly that is required
            for the initiation of photoreceptor outer segment disk formation
  JOURNAL   Proc Natl Acad Sci U S A 117 (18), 9922-9931 (2020)
   PUBMED   32312818
  REMARK    GeneRIF: our work suggests that PCARE is an actin-associated
            protein that interacts with WASF3 to regulate the actin-driven
            expansion of the ciliary membrane at the initiation of new outer
            segment disk formation.
REFERENCE   4  (bases 1 to 4965)
  AUTHORS   Qin,H., Lu,S., Thangaraju,M. and Cowell,J.K.
  TITLE     Wasf3 Deficiency Reveals Involvement in Metastasis in a Mouse Model
            of Breast Cancer
  JOURNAL   Am J Pathol 189 (12), 2450-2458 (2019)
   PUBMED   31542393
  REMARK    GeneRIF: Elevated WASF3 Expression Promotes Metastasis in Breast
            Cancer.
REFERENCE   5  (bases 1 to 4965)
  AUTHORS   Lindstrom,N.O., Guo,J., Kim,A.D., Tran,T., Guo,Q., De Sena
            Brandine,G., Ransick,A., Parvez,R.K., Thornton,M.E., Baskin,L.,
            Grubbs,B., McMahon,J.A., Smith,A.D. and McMahon,A.P.
  TITLE     Conserved and Divergent Features of Mesenchymal Progenitor Cell
            Types within the Cortical Nephrogenic Niche of the Human and Mouse
            Kidney
  JOURNAL   J Am Soc Nephrol 29 (3), 806-824 (2018)
   PUBMED   29449449
REFERENCE   6  (bases 1 to 4965)
  AUTHORS   Yamazaki,D., Suetsugu,S., Miki,H., Kataoka,Y., Nishikawa,S.,
            Fujiwara,T., Yoshida,N. and Takenawa,T.
  TITLE     WAVE2 is required for directed cell migration and cardiovascular
            development
  JOURNAL   Nature 424 (6947), 452-456 (2003)
   PUBMED   12879075
REFERENCE   7  (bases 1 to 4965)
  AUTHORS   Sossey-Alaoui,K., Head,K., Nowak,N. and Cowell,J.K.
  TITLE     Genomic organization and expression profile of the human and mouse
            WAVE gene family
  JOURNAL   Mamm Genome 14 (5), 314-322 (2003)
   PUBMED   12856283
REFERENCE   8  (bases 1 to 4965)
  AUTHORS   Dahl,J.P., Wang-Dunlop,J., Gonzales,C., Goad,M.E., Mark,R.J. and
            Kwak,S.P.
  TITLE     Characterization of the WAVE1 knock-out mouse: implications for CNS
            development
  JOURNAL   J Neurosci 23 (8), 3343-3352 (2003)
   PUBMED   12716942
REFERENCE   9  (bases 1 to 4965)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4965)
  AUTHORS   Sossey-Alaoui,K., Su,G., Malaj,E., Roe,B. and Cowell,J.K.
  TITLE     WAVE3, an actin-polymerization gene, is truncated and inactivated
            as a result of a constitutional t(1;13)(q21;q12) chromosome
            translocation in a patient with ganglioneuroblastoma
  JOURNAL   Oncogene 21 (38), 5967-5974 (2002)
   PUBMED   12185600
  REMARK    GeneRIF: WAVE3 transcript is mainly expressed in the nervous system
            and, like all the members of the WASP gene family, WAVE3 is a key
            element in actin polymerization and cytoskeleton organization.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC115019.28 and AC093356.15.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219383.82230.1,
                                           SRR7345562.1104347.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC115019.28        122575-122638
            65-211              AC115019.28        159218-159364
            212-354             AC115019.28        172625-172767
            355-489             AC093356.15        81593-81727
            490-643             AC093356.15        83038-83191
            644-761             AC093356.15        85266-85383
            762-937             AC093356.15        90687-90862
            938-1204            AC093356.15        96520-96786
            1205-1569           AC093356.15        97937-98301
            1570-4965           AC093356.15        99942-103337
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 85.23 cM"
     gene            1..4965
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /note="WASP family, member 3"
                     /db_xref="GeneID:245880"
                     /db_xref="MGI:MGI:2658986"
     exon            1..64
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            65..211
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            212..354
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..1727
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /note="isoform a is encoded by transcript variant 2;
                     wiskott-Aldrich syndrome protein family member 3; protein
                     WAVE-3; WASP family protein member 3; WAS protein family,
                     member 3; actin-binding protein WASF3"
                     /codon_start=1
                     /product="actin-binding protein WASF3 isoform a"
                     /protein_id="NP_001405168.1"
                     /db_xref="GeneID:245880"
                     /db_xref="MGI:MGI:2658986"
                     /translation="MPLVKRNIEPRHLCRGALPEGVTSELECVTNSTLAAIIRQLSSL
                     SKHAEDIFGELFNEANNFYIRANSLQDRIDRLAVKVTQLDSTVEEVSLQDINMKKAFK
                     SSTIQDQQVVSKNSIPNPVADIYNQSDKPPPLSILTPYRDDKKDGLKFYTDPSYFFDL
                     WKEKMLQDTEDKRKEKRRQKEQKRVDGTTREVKKVRKARNRRQEWNMMAYDKELRPDN
                     RLSQSVHHGASSEGSLSPDTRSHTSDVTDYSYPATPNHALQAQPATPSYTAGDAPLHG
                     TTNQGAEHEYRPSSASARHMALNRPQQPPPPPPPQAPEGSQASTSVAPADYGMLPAQI
                     IEYYSPSGPPPPPPPPMIPSAQTAFVSPLQMPTQPPFPASAVSTYPTPPHQPSTGLLA
                     TAPPPPGPPPPPPGPPGPSSLSSSPMHGPPVAEAKRPEPAQPPISDARSDLLAAIRMG
                     IQLKKVQEQREQEAKREPVGNDVATILSRRIAVEYSDSDDDSEFDENDWSD"
     exon            355..489
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            490..643
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            644..761
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            762..937
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1204
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1569
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..4965
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4942..4947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /note="hexamer: AATAAA"
     polyA_site      4965
                     /gene="Wasf3"
                     /gene_synonym="Scar3; Wave3"
                     /note="major polyA site"
ORIGIN      
        1 gcgtctgtcg ccgctgcgcg ctgcggtgcg ggcggcagga cgatgaagcc gggagcgcgg
       61 cgcgctctga tcccgcttgc tgttcctact gacacctgag ccgcggtggc gatgctgctg
      121 aggcccttcc agcccacgag tcaacctact tggtaggagt gtcttttagc ctcaaggctc
      181 ctcaagtgca ggaagagagt gatctagaaa tattgtcagc catgccttta gtgaagagaa
      241 acatcgaacc ccggcacctg tgccggggcg ccctgccgga aggagtcacc agtgagctgg
      301 aatgcgtgac caacagcacg ctcgctgcaa tcatccgcca gctgagcagt ctgagtaaac
      361 atgcagaaga catatttggt gagttattta atgaggctaa caacttctac atcagagcaa
      421 attcccttca agacagaatc gatcggcttg ctgtcaaagt cacccagctg gactccacgg
      481 tggaggaggt ctcactacag gatatcaaca tgaagaaagc attcaaaagc tctaccatcc
      541 aagaccagca ggtggtctcc aagaacagca ttcccaaccc tgttgctgac atttataatc
      601 agagtgacaa acccccgccg ctgagcattc tgacaccgta cagagatgac aagaaggatg
      661 ggctgaagtt ctacacggat ccctcttact tctttgacct ctggaaagaa aagatgctgc
      721 aggacacaga agacaagagg aaagagaaac gacgtcagaa ggaacaaaag cgtgtagatg
      781 gcaccacccg agaggtgaaa aaggttagaa aagccagaaa caggcgccag gagtggaata
      841 tgatggcgta tgacaaggag cttaggcccg acaacaggtt gtctcagagt gtgcaccacg
      901 gagcatcctc cgagggatct ctgtccccag atactaggtc tcacacatcc gatgtcaccg
      961 attattctta tcctgctact cccaaccatg ctttgcaagc acagccggcg acaccctcct
     1021 acacagctgg cgatgcacca ctgcatggga ccacaaacca aggcgctgag cacgagtacc
     1081 gaccgtcgtc tgcctcggca aggcacatgg ccctgaacag acctcagcag ccaccgccac
     1141 cgcctcctcc ccaggcgccg gaggggtccc aggcctctac gtcagtggct ccagccgact
     1201 atgggatgct tccagcacag ataattgaat actacagccc ctcaggaccg ccaccacctc
     1261 cccctccacc catgattccc tcagcacaaa ctgccttcgt cagccctctc cagatgccca
     1321 cacagccccc gttccctgcc tcagccgtct ccacataccc cactcctcct caccagccct
     1381 ccactgggct cctggccaca gcaccaccac ccccaggtcc cccacctcct ccacctggtc
     1441 ctcctggccc ctcctcccta tcatcgtccc caatgcatgg tccccctgtg gctgaggcaa
     1501 agcgcccaga gcctgcacag ccacccataa gtgatgcgag aagtgacctc ttggctgcca
     1561 tccggatggg aatccaactg aaaaaggtgc aggagcaacg cgagcaggaa gccaagcggg
     1621 agcctgtcgg gaatgacgtg gccaccatct tgtctagacg cattgctgtg gagtacagcg
     1681 actcagacga cgactctgag tttgatgaga atgactggtc tgactgaggc acaagggcca
     1741 ggcgaccagt ggacagccag ggccccatat gcaagtgtgt taggagctcc tgtgctctct
     1801 gcaccctcac agtggtattt ccctgtagct ttagtccctt ttgtattaac agtgtgattt
     1861 gcttctgcac atccttctgg atttgttgag tatttactgt gtaatactta agtgccacag
     1921 aacacagcac accgtgctgc acaccaggaa actcgcagta ttagtgcatc accctggaac
     1981 agcatcttgc ttccctctca gccgtgagag tcccctctcc tgtccatcct gatttggtgg
     2041 gcttgccacg tatctgcgca gctgccccac gtcagctctc tgcaagatca ctggctgtgt
     2101 gtctcagtga tgtcttccaa ccagtgggtg atacgttttc ctgtttcttc cctttgcaaa
     2161 ttgaattcca ggtagatatg gttgtagagg ggaaagttta acaaatcaga atgaaatgtt
     2221 tgtctgtttg cacactgggc ctggtggcct ccaaggaaat gtgtacgatc gtttgctttc
     2281 tctatcttgg cgcttgcaac cgagagcggc catttttatg aggggagccc tgtgggtact
     2341 catgtggaaa gtgcccactg actttggcag atggagttca ctttcatggc tagccctgat
     2401 agcgttgggc accagttagc caggccatgt tcaagctgcc tagagtctgc agagcttgca
     2461 tggcagagcg gccgcatctg gttccctcct actccaccca gtggaaaaga gacctccacg
     2521 ttttattttc tgggatccct gctcctctgt tcaccaggga gtgagcacag gtcagcacct
     2581 cacccccctt gctctgaatc atggaagctt ggctttagct gagagatgcc accagctgtt
     2641 cacaacccat ggtgctgtgg ctagattcct gctcagtctc tcaaacaggg ccacacccct
     2701 cctgaagcac agctctcttc tctcctgttg tcggcacatt ttcaatttta aaaagtacct
     2761 cacaagataa acttgattca gccagttgcc cgggcagaga cctgacttcc cctctcctgc
     2821 cctgatcctt tttaccaaat ggcgattcca tagtcctggc tgaggcaggg gctcaaagtc
     2881 cagtgtctgc tttgcaattc tggaaggaca gctgcttatt taaataagtc tgtgatatcc
     2941 gcaggcttgg gttacaggga ggaccttgca gagggaccag gacactgcct tcccttaggt
     3001 gcattattta cgtggcctgc agctcttaat atctctcact gtttatgaaa agaaagattc
     3061 agatccgggg tgacattgtg tcagttgaca gggaatgcct gtgggataat taaaaatggt
     3121 ttttgctcat gagtttggga tgtcacccag ccgcccacat cgcctgctat gtgtgtggac
     3181 gcctgtccgc ttggccagct atcctgtaac acctgccagt agtaggtttt cctcatgtca
     3241 ctatgacagt gaaagcccat gtgcaagccc ctccctacgc cctgctttgg cgtgtgtctg
     3301 agtgctctgt gctgcgccat ggccatcctg gagcctgcgc acacgtggcc gctgctgtga
     3361 ctcgctgtcc ttggtaattg gtgagaggct ttttctttct ttctcagttc tgcttttcca
     3421 cactgagacc caccatgttg acatcatcct cgcgacgttg acatttgagc cttttgttct
     3481 gtctcccatg tgccctgtag aacccagcac tcatgtttgg caaaactttt tttggtaagg
     3541 tccttatgct ctcagggtgg agggaagaca ttaaagctac gcagccattg tcaggatttt
     3601 ttctactgat gtcacatcag aatataacta gtggctcctt actcacaaat gttcacgagc
     3661 ttctccgttg tcacttgtcc tgtagttttg ccaacaagtt aattttagtt tgattttata
     3721 gctagagaga aagcttttgc ccgttgcatt ctgtaacctt caaaagaatg agaaacgact
     3781 aaaatccaat tcagattatt tttagagatt ctttttggca aatttgattt agtctaaaat
     3841 ttaaattcaa gttgcttgaa tactttacaa aacgagaaca aagtaaaaag tttagatttt
     3901 tgatttccac ttttttttta aaaagtacta aaacaaatgg agagcagaca tagtagcata
     3961 tttgacttct acacatttca tgcttatact taatctacag atcgcaaggt tgttttatat
     4021 tatcccctta tagtgttcca tgtgtattgt agctgagttt cagatacggt attttctcat
     4081 agtgtaaact caggcagatg aatagctgga gagtgggtgg atggatgggt gagtatatgg
     4141 gtagataaat ggatgagtgg atgggtgggt taggtgggta agtagctagg tggatgggtg
     4201 ggtggataca tggatgagtg gatgggtgga ttggtgggtg agtggatagg tgaatggatg
     4261 ataggtggtg gatggttgag tgggtggatt catgatggat ggatggatgg atggatggat
     4321 ggatggatgg atgagtggat ggataatagg ttgatgggtg gataggtaga tgagtgaata
     4381 gatagatata gatcagtggg aggattggaa agtaggtgaa tgaatggatg gcagatagat
     4441 ggatcaatta cagatggatg gatgcatgga atagataggt ggatgggtgg tggctagaag
     4501 gtgcctgagt ctgcacatca ctgcactgag aaggcaaatg agtacattat actattagca
     4561 ccaaacccca agcttcagta tttagatttt atttactaag atgctattag aagttggatg
     4621 ttttcatccc agtacatttt taacacatca aatcatttat gagcatagga tgatgagggt
     4681 gtgaaagctt tgtaatattt tcagcagaaa tgcgtgatac atttgaatcc attgaggtct
     4741 ggagggaggg tatcagccag agtagcaagg cggttttgtt tatttgtttg tttttttgtt
     4801 tgtttgtttt atgaaggaga gtatctgtta tcttgcagta ttatcaatgc agttgtaaat
     4861 tgaatctaaa gtggtattta aaactgtcaa acaattttta atctgtttgt atatcttatt
     4921 atagatcaca agtaacatct aaataaaatt acacgtgtaa cccta
//
