LOCUS       NM_009515               2094 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus Wiskott-Aldrich syndrome (Was), mRNA.
ACCESSION   NM_009515 XM_976759
VERSION     NM_009515.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Bhanja,A., Seeley-Fallen,M.K., Lazzaro,M., Upadhyaya,A. and Song,W.
  TITLE     N-WASP-dependent branched actin polymerization attenuates B-cell
            receptor signaling by increasing the molecular density of receptor
            clusters
  JOURNAL   Elife 12, 87833 (2023)
   PUBMED   38085658
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Liang,F., Peng,C., Luo,X., Wang,L., Huang,Y., Yin,L., Yue,L.,
            Yang,J. and Zhao,X.
  TITLE     A single-cell atlas of immunocytes in the spleen of a mouse model
            of Wiskott-Aldrich syndrome
  JOURNAL   Cell Immunol 393-394, 104783 (2023)
   PUBMED   37944382
REFERENCE   3  (bases 1 to 2094)
  AUTHORS   Liang,F., Yang,J., Gan,Q., Xia,Y., Wang,L., Huang,Y. and Peng,C.
  TITLE     Transcriptomic insights into the role of the spleen in a mouse
            model of Wiskott-Aldrich syndrome
  JOURNAL   Exp Ther Med 25 (1), 64 (2022)
   PUBMED   36605531
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2094)
  AUTHORS   Descatoire,M., Fritzen,R., Rotman,S., Kuntzelman,G., Leber,X.C.,
            Droz-Georget,S., Thrasher,A.J., Traggiai,E. and Candotti,F.
  TITLE     Critical role of WASp in germinal center tolerance through
            regulation of B cell apoptosis and diversification
  JOURNAL   Cell Rep 38 (10), 110474 (2022)
   PUBMED   35263577
REFERENCE   5  (bases 1 to 2094)
  AUTHORS   Li,W., Jia,Y., Wang,Y., Zhao,Q., Yang,L., Zeng,T., Niu,L., Dai,R.,
            Li,Y., Zhao,X. and Wu,J.
  TITLE     WASp Deficiency Selectively Affects the TCR Diversity of Different
            Memory T Cell Subsets in WAS Chimeric Mice
  JOURNAL   Front Immunol 12, 794795 (2022)
   PUBMED   35116029
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2094)
  AUTHORS   Snapper,S.B., Rosen,F.S., Mizoguchi,E., Cohen,P., Khan,W.,
            Liu,C.H., Hagemann,T.L., Kwan,S.P., Ferrini,R., Davidson,L.,
            Bhan,A.K. and Alt,F.W.
  TITLE     Wiskott-Aldrich syndrome protein-deficient mice reveal a role for
            WASP in T but not B cell activation
  JOURNAL   Immunity 9 (1), 81-91 (1998)
   PUBMED   9697838
REFERENCE   7  (bases 1 to 2094)
  AUTHORS   Wu,Y., Spencer,S.D. and Lasky,L.A.
  TITLE     Tyrosine phosphorylation regulates the SH3-mediated binding of the
            Wiskott-Aldrich syndrome protein to PSTPIP, a
            cytoskeletal-associated protein
  JOURNAL   J Biol Chem 273 (10), 5765-5770 (1998)
   PUBMED   9488710
REFERENCE   8  (bases 1 to 2094)
  AUTHORS   Quilliam,L.A., Lambert,Q.T., Mickelson-Young,L.A., Westwick,J.K.,
            Sparks,A.B., Kay,B.K., Jenkins,N.A., Gilbert,D.J., Copeland,N.G.
            and Der,C.J.
  TITLE     Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein
            and potential effector of Rho protein signaling
  JOURNAL   J Biol Chem 271 (46), 28772-28776 (1996)
   PUBMED   8910519
REFERENCE   9  (bases 1 to 2094)
  AUTHORS   Schindelhauer,D., Hellebrand,H., Grimm,L., Bader,I., Meitinger,T.,
            Wehnert,M., Ross,M. and Meindl,A.
  TITLE     Long-range map of a 3.5-Mb region in Xp11.23-22 with a
            sequence-ready map from a 1.1-Mb gene-rich interval
  JOURNAL   Genome Res 6 (11), 1056-1069 (1996)
   PUBMED   8938429
REFERENCE   10 (bases 1 to 2094)
  AUTHORS   Derry,J.M., Wiedemann,P., Blair,P., Wang,Y., Kerns,J.A.,
            Lemahieu,V., Godfrey,V.L., Wilkinson,J.E. and Francke,U.
  TITLE     The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP)
            gene is highly conserved and maps near the scurfy (sf) mutation on
            the X chromosome
  JOURNAL   Genomics 29 (2), 471-477 (1995)
   PUBMED   8666397
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY232461.1, U29673.1 and CJ237505.1.
            
            On Oct 30, 2009 this sequence version replaced NM_009515.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U29673.1, U54788.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                BY232461.1         2-40
            40-1691             U29673.1           1-1652
            1692-2094           CJ237505.1         109-511
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 3.65 cM"
     gene            1..2094
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /note="Wiskott-Aldrich syndrome"
                     /db_xref="GeneID:22376"
                     /db_xref="MGI:MGI:105059"
     exon            1..201
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1626
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /note="wiskott-Aldrich syndrome protein homolog;
                     Wiskott-Aldrich syndrome homolog"
                     /codon_start=1
                     /product="actin nucleation-promoting factor WAS"
                     /protein_id="NP_033541.1"
                     /db_xref="CCDS:CCDS29984.1"
                     /db_xref="GeneID:22376"
                     /db_xref="MGI:MGI:105059"
                     /translation="MNSGPGPVGGRPGGRGGPAVQQNIPSNLLQDHENQRLFELLGRK
                     CWTLATTVVQLYLALPPGAEHWTMEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQE
                     LYSQLVYLTPTPFFHTFAGDDCQVGLNFADESEAQAFRALVQEKIQKRNQRQSGERRQ
                     LPPPPAPINEERRGGLPPVPPHPGGDHGGPSGGPLSLGLVTVDIQNPDITSSRYRGLP
                     APGPGPTDKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRA
                     GISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPCRGGGGGGGGGGG
                     GGGGGGGQPLRPPVVGSNKGRSGPLPPVPMGGAPPPPTPRGPPPPGRGGPPPPPPPAT
                     GRSGPPPPPLPGAGGPPAPPPPPPPPPPPPCPGSGPAPPPLPPTPVSGGSPAPGGGRG
                     ALLDQIRQGIQLNKTPGALENSVQQPPAQQSEGLVGALMHVMQKRSRVIHSSDEGEDQ
                     TGEDEEDDEWDD"
     exon            202..342
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            343..429
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            430..532
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            533..574
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            575..628
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            629..803
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            804..846
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            847..1000
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1455
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1570
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..2094
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1789..1794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /note="hexamer: GATAAA"
     polyA_site      1808
                     /gene="Was"
                     /gene_synonym="Wasp"
                     /note="major polyA site"
ORIGIN      
        1 ggaagttcct cttcttaccc tgcacccaga gcttagctgg agaggactaa ggcagaaggc
       61 agcatgaata gtggccctgg ccctgtagga ggcaggcctg ggggccgagg gggaccagcc
      121 gttcagcaga acattccttc caacctcctc caggaccatg aaaaccagag actctttgag
      181 cttcttggcc gaaaatgctg gacactggct accacagttg ttcagctcta cctggcactg
      241 ccccctggag ctgagcactg gaccatggaa cactgcgggg ctgtgtgctt cgtgaaggat
      301 aaccctcaga agtcctactt catccgcctt tatggcctac aggctggtcg gctactctgg
      361 gaacaggagc tgtactctca gctggtttat ctcactccca ccccgttctt ccacactttt
      421 gctggagatg actgtcaagt aggactgaac tttgcggatg agagtgaagc ccaggccttc
      481 cgggccttgg tgcaggagaa gatacaaaaa aggaatcaga ggcaaagcgg agaaagacgc
      541 cagctaccac caccaccagc accaatcaat gaggagagaa gaggagggct cccacctgtg
      601 cccccacacc cgggtggaga tcatgggggc ccatcaggtg gtccactatc tctaggactt
      661 gtgacggtcg acattcagaa ccctgacatc acaagttcac gataccgtgg gctccctgca
      721 cctggccctg gcccaactga taagaaacgc tcagggaaaa agaagatcag caaagctgat
      781 atcggagcac cgagtggatt caaacatgtc agccacgtgg gctgggatcc ccagaatgga
      841 tttgatgtga acaacctaga cccggatctg cggagcttgt tctccagggc aggaatcagc
      901 gaggcccagc tcactgacgc agagacctcc aagctcatct acgattttat tgaggaccag
      961 ggaggtctag aggctgtccg gcaggagatg aggcgccaag agccactccc accacctccg
     1021 ccgccatgca gaggaggagg aggaggagga ggaggaggag gaggaggagg aggaggagga
     1081 ggaggccagc ctctgagacc tcctgttgtg gggagtaata agggtcgctc aggtccactg
     1141 ccccctgtac ctatgggggg tgccccacct ccaccaacac cacgagggcc cccaccacca
     1201 ggccgagggg gtcctcctcc accaccccct ccagccactg gacgatctgg accaccacct
     1261 cctccactcc ctggagctgg gggaccacca gcaccgccac caccaccacc accaccacca
     1321 cctccaccct gccctgggag tgggcccgcc cctcccccgc tccctcctac tccagtgtct
     1381 ggggggagcc cagcacctgg tgggggccgg ggtgcacttt tggaccaaat ccggcaggga
     1441 attcagctga acaagacccc tggagctcta gagaactcag tacagcaacc acccgcgcag
     1501 cagtcagaag gcctagtagg tgccctgatg catgtcatgc agaagaggag tagagtcatc
     1561 cattcctcag atgaagggga ggatcagacc ggcgaggatg aagaggatga tgaatgggat
     1621 gactaaagtc atcttccttc cagcaagcca gttcctctcc acactcactc tgccatctag
     1681 attctgctcc gctggcagct tcccaattca cctgttgggg aacctcatac ccaatctaaa
     1741 gtacacatga cgtcaccttt cacctctcat aactcaggga tgaaacagga taaaattgag
     1801 tctatgtgtc tgagtgtgtg ttcattcaca tcctaaatga gtagtttggg tttctttccc
     1861 tcacagtccc ttttggcttt gatcttgctt tgtgtgtttt tattgagcct ttcacaagta
     1921 tgatcattta ttcctttaag atttcagcca tagccgggtg tggtggcgca cgcctttaat
     1981 tccagcagaa gggaggcaga ggcaggtgga tttctgagtt cgagaccagc ctggtctaca
     2041 gagtgagttc caggacagcc agggctatac agagaaaccc tgtctcaaaa aacc
//
