LOCUS       NM_001411909            1867 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus tryptophanyl-tRNA synthetase1 (Wars1), transcript
            variant 6, mRNA.
ACCESSION   NM_001411909
VERSION     NM_001411909.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Li,X., Zhang,F., Sun,L., Cai,X., Lou,F., Sun,Y., Gao,M., Wang,Z.,
            Tang,S., Fan,L., Wu,Y., Jin,X., Deng,S., Xu,Z., Sun,X., Li,Q. and
            Wang,H.
  TITLE     Single-Cell RNA Sequencing Identifies WARS1+ Mesenchymal Stem Cells
            with Enhanced Immunomodulatory Capacity and Improved Therapeutic
            Efficacy
  JOURNAL   J Immunol 213 (3), 257-267 (2024)
   PUBMED   38856632
  REMARK    GeneRIF: Single-Cell RNA Sequencing Identifies WARS1+ Mesenchymal
            Stem Cells with Enhanced Immunomodulatory Capacity and Improved
            Therapeutic Efficacy.
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1867)
  AUTHORS   Kim,Y.T., Huh,J.W., Choi,Y.H., Yoon,H.K., Nguyen,T.T., Chun,E.,
            Jeong,G., Park,S., Ahn,S., Lee,W.K., Noh,Y.W., Lee,K.S., Ahn,H.S.,
            Lee,C., Lee,S.M., Kim,K.S., Suh,G.J., Jeon,K., Kim,S. and Jin,M.
  TITLE     Highly secreted tryptophanyl tRNA synthetase 1 as a potential
            theranostic target for hypercytokinemic severe sepsis
  JOURNAL   EMBO Mol Med 16 (1), 40-63 (2024)
   PUBMED   38177528
  REMARK    GeneRIF: Highly secreted tryptophanyl tRNA synthetase 1 as a
            potential theranostic target for hypercytokinemic severe sepsis.
            Erratum:[EMBO Mol Med. 2024 Mar;16(3):665. doi:
            10.1038/s44321-024-00030-4.. PMID: 38316935]
REFERENCE   4  (bases 1 to 1867)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1867)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1867)
  AUTHORS   Paley,E.L., Denisova,G., Sokolova,O., Posternak,N., Wang,X. and
            Brownell,A.L.
  TITLE     Tryptamine induces tryptophanyl-tRNA synthetase-mediated
            neurodegeneration with neurofibrillary tangles in human cell and
            mouse models
  JOURNAL   Neuromolecular Med 9 (1), 55-82 (2007)
   PUBMED   17114825
  REMARK    GeneRIF: TrpRS plays a role in protecting against neurodegeneration
            is suggested, providing an insight into the pathogenesis and a
            possible treatment of neurodegenerative diseases.
REFERENCE   7  (bases 1 to 1867)
  AUTHORS   Sekita,Y., Wagatsuma,H., Irie,M., Kobayashi,S., Kohda,T.,
            Matsuda,J., Yokoyama,M., Ogura,A., Schuster-Gossler,K., Gossler,A.,
            Ishino,F. and Kaneko-Ishino,T.
  TITLE     Aberrant regulation of imprinted gene expression in Gtl2lacZ mice
  JOURNAL   Cytogenet Genome Res 113 (1-4), 223-229 (2006)
   PUBMED   16575184
REFERENCE   8  (bases 1 to 1867)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   9  (bases 1 to 1867)
  AUTHORS   Pajot,B., Sarger,C., Bonnet,J. and Garret,M.
  TITLE     An alternative splicing modifies the C-terminal end of
            tryptophanyl-tRNA synthetase in murine embryonic stem cells
  JOURNAL   J Mol Biol 242 (4), 599-603 (1994)
   PUBMED   7932716
REFERENCE   10 (bases 1 to 1867)
  AUTHORS   Kisselev,L.L.
  TITLE     Mammalian tryptophanyl-tRNA synthetases
  JOURNAL   Biochimie 75 (12), 1027-1039 (1993)
   PUBMED   7515282
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122811.4 and AC152061.6.
            
            Transcript Variant: Variants 1, 4, 5, and 6 encode the same isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.8390739.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849380,
                                           SAMN00849381 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-81                AC122811.4         77489-77569         c
            82-254              AC122811.4         71800-71972         c
            255-468             AC122811.4         66119-66332         c
            469-577             AC122811.4         64467-64575         c
            578-697             AC122811.4         59090-59209         c
            698-880             AC122811.4         58538-58720         c
            881-981             AC122811.4         51101-51201         c
            982-1268            AC152061.6         205010-205296       c
            1269-1409           AC152061.6         201800-201940       c
            1410-1567           AC152061.6         200266-200423       c
            1568-1867           AC152061.6         199031-199330       c
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 59.66 cM"
     gene            1..1867
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /note="tryptophanyl-tRNA synthetase1"
                     /db_xref="GeneID:22375"
                     /db_xref="MGI:MGI:104630"
     exon            1..81
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            82..254
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1589
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /EC_number="6.1.1.2"
                     /note="isoform 1 is encoded by transcript variant 6;
                     tryptophanyl-tRNA synthetase, cytoplasmic;
                     tryptophan--tRNA ligase, cytoplasmic"
                     /codon_start=1
                     /product="tryptophan--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_001398838.1"
                     /db_xref="GeneID:22375"
                     /db_xref="MGI:MGI:104630"
                     /translation="MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAV
                     KMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGI
                     DYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPF
                     YLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSY
                     TVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDS
                     DCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHP
                     KPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHR
                     QFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQ
                     ARRKAVTEETVKEFMTPRQLSFHFQCFCFDT"
     exon            255..468
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            469..577
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            578..697
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            698..880
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            881..981
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1268
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1409
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1567
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1867
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1848..1853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /note="hexamer: AATAAA"
     polyA_site      1867
                     /gene="Wars1"
                     /gene_synonym="TrpRS; Wars; WRS"
                     /note="major polyA site"
ORIGIN      
        1 gtttccacgg acgcccaaga ggtaccggtg gctgaacttg ggggtgctga ctggtggggc
       61 cgaagtgggg gccgccggta aagtcctgtc tcaactgctg aacacactac tcacaagcca
      121 gctgcaggcc tcggtcctag aagatggcag acatgcccag tggcgagtcc tgcacctctc
      181 cactggagct gttcaacagc atagctactc aaggggagct cgtgaggtcc ctcaaagctg
      241 gaaatgcacc aaaagatgag attgattctg cagtaaagat gttgttatca ttaaaaatga
      301 gctataaggc cgccatgggg gaggagtaca aggctggctg ccctcctggg aacccaacag
      361 ctgggagaaa ttgtgactct gatgccacaa aagccagcga ggattttgtg gatccatgga
      421 cggtgcggac aagcagtgca aaaggcatcg actatgacaa gctcattgtt cagtttggaa
      481 gcagcaaaat tgacaaagag ctgataaacc gaatagagcg agccactggc caaagaccgc
      541 accgcttcct gcgccggggc atcttcttct cccaccgaga tatgaatcaa attctggatg
      601 cctatgaaaa taagaagcca ttctacctgt acacaggcag gggcccctcc tccgaagcaa
      661 tgcatctggg tcatctcgtc ccgtttattt tcacaaagtg gctacaggat gtgttcaatg
      721 tgcctttggt catccagatg tctgacgacg agaagtacct gtggaaggac ttgactctgg
      781 agcaggcata tagctacacc gtagaaaatg caaaggacat cattgcctgt ggcttcgaca
      841 tcaacaaaac tttcatcttc tctgatcttg agtatatggg gcagagcccg ggcttctaca
      901 ggaacgtggt gaagattcag aagcacgtca ccttcaacca agtgaaaggc attttcggct
      961 ttacggacag tgactgcatc gggaagatca gttttcctgc tgtccaggcc gcgccatcgt
     1021 tcagcaactc tttcccgaag atcttccgag acaggacaga tatccagtgc ctcatcccgt
     1081 gtgccattga ccaggatccg tacttcagaa tgacaaggga cgtggccccc aggatcggcc
     1141 atcctaaacc tgccctgctg cactcgacct tcttccctgc cctgcagggc gctcagacca
     1201 agatgagtgc cagtgacccc aactcctcca tcttcctcac tgacacggcc aagcagatca
     1261 agagcaaggt caacaagcat gcattttctg gaggaagaga tactgtggag gagcacaggc
     1321 agtttggggg caactgtgaa gtggatgtat ccttcatgta tctgaccttc ttccttgaag
     1381 atgacgacag gctggagcag atcagaaagg attacaccag cggagccatg ctcaccgggg
     1441 agctcaagaa gacgctcata gacgtccttc agcccctgat cgcagagcac caggcccgac
     1501 gcaaggcggt cactgaggag accgtgaagg agttcatgac gccccggcag ctgtccttcc
     1561 acttccagtg cttctgcttt gacacttgac tgacggggag ggcaggccag agcagtgtgc
     1621 tggccatgcc tcaggccgtt gaccatagct agacagaaaa gaacagcagc cgtccaggcc
     1681 gcagcgttct ccctctgggc gggctgcatg gaatgagcct ccactcagag tcttggaaaa
     1741 gcagaagccg tacctcaggg aaagcccctg gttgaagtgt ctgtcctctc tgaaggcaca
     1801 agggccactc tgagtgttct gtccttgcat catttttagg aaaaaacaat aaactttgtt
     1861 gagctcc
//
