LOCUS       NM_001004436            6371 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus WAPL cohesin release factor (Wapl), transcript variant
            1, mRNA.
ACCESSION   NM_001004436 XM_127654
VERSION     NM_001004436.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Kiefer,L., Chiosso,A., Langen,J., Buckley,A., Gaudin,S.,
            Rajkumar,S.M., Servito,G.I.F., Cha,E.S., Vijay,A., Yeung,A.,
            Horta,A., Mui,M.H. and Canzio,D.
  TITLE     WAPL functions as a rheostat of Protocadherin isoform diversity
            that controls neural wiring
  JOURNAL   Science 380 (6651), eadf8440 (2023)
   PUBMED   37347873
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Kean,C.M., Tracy,C.J., Mitra,A., Rahat,B., Van Winkle,M.T.,
            Gebert,C.M., Noeker,J.A., Calof,A.L., Lander,A.D., Kassis,J.A. and
            Pfeifer,K.
  TITLE     Decreasing Wapl dosage partially corrects embryonic growth and
            brain transcriptome phenotypes in Nipbl+/- embryos
  JOURNAL   Sci Adv 8 (48), eadd4136 (2022)
   PUBMED   36449618
  REMARK    GeneRIF: Decreasing Wapl dosage partially corrects embryonic growth
            and brain transcriptome phenotypes in Nipbl[+/-] embryos.
REFERENCE   3  (bases 1 to 6371)
  AUTHORS   Hsieh,T.S., Cattoglio,C., Slobodyanyuk,E., Hansen,A.S., Darzacq,X.
            and Tjian,R.
  TITLE     Enhancer-promoter interactions and transcription are largely
            maintained upon acute loss of CTCF, cohesin, WAPL or YY1
  JOURNAL   Nat Genet 54 (12), 1919-1932 (2022)
   PUBMED   36471071
  REMARK    GeneRIF: Enhancer-promoter interactions and transcription are
            largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1.
REFERENCE   4  (bases 1 to 6371)
  AUTHORS   Chatzidaki,E.E., Powell,S., Dequeker,B.J.H., Gassler,J.,
            Silva,M.C.C. and Tachibana,K.
  TITLE     Ovulation suppression protects against chromosomal abnormalities in
            mouse eggs at advanced maternal age
  JOURNAL   Curr Biol 31 (18), 4038-4051 (2021)
   PUBMED   34314679
REFERENCE   5  (bases 1 to 6371)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6371)
  AUTHORS   Tedeschi,A., Wutz,G., Huet,S., Jaritz,M., Wuensche,A.,
            Schirghuber,E., Davidson,I.F., Tang,W., Cisneros,D.A., Bhaskara,V.,
            Nishiyama,T., Vaziri,A., Wutz,A., Ellenberg,J. and Peters,J.M.
  TITLE     Wapl is an essential regulator of chromatin structure and
            chromosome segregation
  JOURNAL   Nature 501 (7468), 564-568 (2013)
   PUBMED   23975099
  REMARK    GeneRIF: in mitosis, Wapl-mediated release of cohesin from DNA is
            essential for proper chromosome segregation and protects cohesin
            from cleavage by the protease separase, thus enabling mitotic exit
            in the presence of functional cohesin complexes
REFERENCE   7  (bases 1 to 6371)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 6371)
  AUTHORS   Kuroda,M., Oikawa,K., Ohbayashi,T., Yoshida,K., Yamada,K.,
            Mimura,J., Matsuda,Y., Fujii-Kuriyama,Y. and Mukai,K.
  TITLE     A dioxin sensitive gene, mammalian WAPL, is implicated in
            spermatogenesis
  JOURNAL   FEBS Lett 579 (1), 167-172 (2005)
   PUBMED   15620708
  REMARK    GeneRIF: mWAPL may be involved in spermatogenesis and be target
            genes mediating the reproductive toxicity induced by
            2,3,7,8-trachlorodibenzo-p-dioxin .
REFERENCE   9  (bases 1 to 6371)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   10 (bases 1 to 6371)
  AUTHORS   Oikawa,K., Ohbayashi,T., Kiyono,T., Nishi,H., Isaka,K., Umezawa,A.,
            Kuroda,M. and Mukai,K.
  TITLE     Expression of a novel human gene, human wings apart-like (hWAPL),
            is associated with cervical carcinogenesis and tumor progression
  JOURNAL   Cancer Res 64 (10), 3545-3549 (2004)
   PUBMED   15150110
  REMARK    GeneRIF: High levels of WAPAL are observed in human cervical
            cancers, and expression of human WAPAL in mice causes tumor
            formation.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ134128.1, BY732412.1,
            BC139434.1, CJ061782.1, DV659439.1, CJ053978.1, DV646537.1,
            BG068179.2 and BP757948.1.
            
            On Aug 6, 2014 this sequence version replaced NM_001004436.3.
            
            Summary: Studies suggest that the protein encoded by this gene is
            important for the release of cohesin from chromatin. This gene
            product is thought to be essential for development, and reduced
            expression of this gene in cells causes defects in chromatin
            structure. High levels of expression of the human ortholog of this
            gene are observed in cervical cancers, and expression of the human
            ortholog of this gene in mice results in tumor formation.
            Alternative splicing results in multiple transcript variants
            encoding different protein isoforms. [provided by RefSeq, Aug
            2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 CJ134128.1         3-3
            2-245               BY732412.1         3-246
            246-4451            BC139434.1         1-4206
            4452-4728           CJ061782.1         74-350
            4729-5175           DV659439.1         35-481
            5176-5591           CJ053978.1         1-416
            5592-5840           DV646537.1         1-249
            5841-6348           BG068179.2         3-510               c
            6349-6371           BP757948.1         1-23                c
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 20.84 cM"
     gene            1..6371
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="WAPL cohesin release factor"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
     exon            1..433
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            434..954
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     CDS             456..4058
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="isoform 1 is encoded by transcript variant 1;
                     friend of EBNA2; wings apart-like protein homolog;
                     dioxin-inducible factor 2; wings apart-like homolog"
                     /codon_start=1
                     /product="wings apart-like protein homolog isoform 1"
                     /protein_id="NP_001004436.2"
                     /db_xref="CCDS:CCDS36880.1"
                     /db_xref="GeneID:218914"
                     /db_xref="MGI:MGI:2675859"
                     /translation="MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKL
                     GQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSE
                     AAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSC
                     TKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNS
                     QSGKRTESPSESCPVKGSVRTGLYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNR
                     IGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFDKLMDGTSQSLAKAN
                     SESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEF
                     TASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEG
                     GSGSSNYKIKYFGFDDLSESEDDDDDDCQVERKKDKKRTKTAPSPSQQPPPESSDNSQ
                     DSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNA
                     RHWNHPDSEELPGPPIAKPQRVTVRLSSKEPNQKDDGVFKAPAPPLKVIKTVTIPTQP
                     YQEIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNT
                     RCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDR
                     LNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENI
                     TTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDDEDEEKLVA
                     SLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIISSAKALQHCEDLIQQYNRAENS
                     ICVADSNPLPYQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQEGLIGTAM
                     NCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMQTSCSFDSSFSSGEG
                     DHSLRLAGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGE
                     IQWVSTEKTDGAEEKQKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQES
                     PINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC"
     exon            955..2007
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2126
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2222
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2375
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2512
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2513..2624
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2852
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..2964
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3065
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3066..3142
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3143..3318
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..3434
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3599
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3600..3807
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3808..3896
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3897..3992
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     exon            3993..6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4562..4567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="hexamer: AATAAA"
     polyA_site      4587
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="major polyA site"
     regulatory      6328..6333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="hexamer: AATAAA"
     polyA_site      6351
                     /gene="Wapl"
                     /gene_synonym="A530089A20Rik; DIF-2; FOE; Wapal"
                     /note="major polyA site"
ORIGIN      
        1 gttggaggaa ggaggtgggg ggccgggagc gcaaatggcg ttgagatggt tcagggccct
       61 gttcaaactc cagcgctgac cattcaccgg cggaggcggc ggcgcagaag gcggcggcgg
      121 cccagcgggg gcacgtagcg ggctcggcac cggcggcggc cgccagggag gcctaggccc
      181 tgtccggccg gcgcgcctga ggtggggagg gaagttgcgg ggccgccgct caccccccac
      241 cccccctgtc gcccgagctt cctatttacc gaagcggagc cgcggactgt gacggcagca
      301 gagcccctcg cccctctcgg tggcaccggt cggcactggt ctctcgcgcg gggctcccgc
      361 gcccgcccgc gggccgttgg gagccggaga ggcggaggcg gcccgaggcc aaagcacccg
      421 ccaggcgccg aggggaatat gaaacaggtg tcaaaatgac atccagattt ggaaaaactt
      481 acagtaggaa aggaggaaat ggcagttcaa aatttgatga agttttttcc aacaaacgga
      541 ctactcttag tacaaaatgg ggtgagacca catttatggc taaattaggg cagaagaggc
      601 ccaatttcaa accagatatt caagaaattc cgaagaaacc taaagtagaa gaagaagata
      661 ctggagatcc ctttggtttt gatagtgatg atgagtctct acctgtttct tcaaaaaatt
      721 tagcccaggg taagggttca tcttactcag aatctagtga ggctgctcag ctggaagaag
      781 tcacttctgt atttgaagct aatagcaaat gtagtcatgt ggtgggtgaa gacagttttg
      841 cttccgacag atgcttactt gtggaggata ctttaattgg gaaagagaag agcataagta
      901 gaattccaga agacaacgca aacaaaagta gttgcactaa gttgctaact tcagataaag
      961 tggagaattt tagtgaagaa catgaaaaaa atagtcacca ctttcacaaa aatgctgaag
     1021 atagtactaa gaaacccaat gcagaaaccg cagtggcttc tgaatataaa gctgatgaaa
     1081 ctaaagaaac aaatgatact tggaactccc agtctggaaa aagaacagag tctccatctg
     1141 aaagttgtcc agtcaaagga tctgtaagaa ctggtttata tgaatgggat aatgattttg
     1201 aagatatcag gtcagaagac tgtattttaa gtttggataa tgagtctctt ttggagatga
     1261 aagacgagga tttaaaaaat cggattggag gattggaaaa tctaaatgaa acctttgaag
     1321 aagatatcat acaaagtgtt cttaggccaa gcaactgtag gacgtactgt agggccaata
     1381 aagcgagatc ctcacaggga gcatcaaatt ttgataagct aatggatggc accagtcagt
     1441 ccttagccaa agcaaacagt gaatcaagta aagatggcct gaatcaggca aagaaaggta
     1501 gtgcaagttg tgggaccagt tttcgaggaa cagttggacg gactagagat tacactgttt
     1561 tacatccatc ttgcttgtca gtgtgtaatg ttaccatcca ggatactatg gaacggagta
     1621 tggatgagtt caccgcatcc actcctgcag atttaggaga ggctggccgg ctcagaaaaa
     1681 aggcagatat tgcaacctcc aagaccacta ctagatttcg acctagtaat actaaatcca
     1741 aaaaggatgt taaacttgaa ttttttggtt ttgaagatca tgatgagaca ggaggtgatg
     1801 aagggggttc tggaagttct aattacaaaa ttaaatattt tggctttgac gatctcagcg
     1861 aaagtgaaga tgatgatgat gacgactgtc aagtggaaag aaagaaagac aaaaaaagaa
     1921 ctaaaacagc tccatcacct tcccagcagc ctcctcctga aagcagcgac aattcccagg
     1981 atagtcagtc tagtactaat aatgcagaaa acttggattt tacagaggac ttgcctggtg
     2041 tgcctgagag tgtgaagaag cccataagta aacaaggaga taaatccaag gaaaatacca
     2101 gaaagatttt tagtggcccc aaacggtcac ctacaaaagc tgtatataat gccaggcatt
     2161 ggaaccatcc agactcggaa gaattgcctg gaccaccaat agcaaaacct cagcgtgtca
     2221 cagtgaggct gtcttcaaag gaaccaaatc aaaaagatga tggagttttt aaggctcctg
     2281 caccaccact caaagtgata aaaactgtga caatacctac tcagccctac caagaaatag
     2341 ttactgcact gaaatgcaga aaagaagaca aagaattata tactgttgtt cagcacgtga
     2401 aacacttcaa tgatgtggtg gaatttggtg aaaatcaaga gttcactgat gacattgaat
     2461 acttgttaag tggcttaaag agtactcagc ctctaaacac acgttgcctt agtgttatca
     2521 gcttagctac taaatgtgcc atgcccagtt ttcggatgca tctgagggca catgggatgg
     2581 ttgcaatggt ctttaaaact ttggatgatt cccagcatca tcagaatctg tccctctgta
     2641 cagctgctct catgtacata ttgagtagag accgtttgaa catggatctt gatagggcca
     2701 gcctagatct catgattcgg cttttggagt tggaacaaga tgcctcttca gctaagctac
     2761 tgaatgaaaa agacatgaac aagatcaaag aaaagatccg aagactctgt gaaactgtgc
     2821 acaacaagca tcttgatcta gaaaacataa cgactggtca tttagctatg gagacattgc
     2881 tgtccctcac ttccaaacga gcaggagatt ggtttaaaga agagctccga cttctgggtg
     2941 gtctggatca tattgtagat aaagtaaaag agtgtgtgga tcatttaagt agagatgatg
     3001 aggacgaaga gaaactagta gcctcattat ggggagcaga gagatgttta cgagttttag
     3061 agagtgtaac agtgcataat ccagagaatc aaagctactt gatagcctat aaagattcac
     3121 aactcattat ttcatcagct aaagcattac agcattgtga agacctgatt cagcagtaca
     3181 accgtgctga gaacagcatc tgtgtagcag acagtaaccc tctgccttac cagaatgtaa
     3241 ctaaccatgt gggcaaagca gtggaggact gcatgagggc tataattgga gtattgctca
     3301 atttaactaa tgataatgag tggggcagca caaagacagg agaacaagaa ggactcatag
     3361 gcacagcgat gaactgtgtt cttcaggttc caaagtacct acctcaggag cagagatttg
     3421 atattcgagt gctgggattg ggtctactca taaacctggt ggagtatagt gcccggaatc
     3481 gacactgcct tgtcaacatg caaacatcct gttcctttga ttcctccttc tctagtggag
     3541 aaggcgatca tagtttaagg ctagccggac aagttcatgc tgttcaagct ttagtgcagc
     3601 tatttctcga acgagagaga gcagcacaat tggcagaaag taaaacagat gaattgatta
     3661 aagatgctcc taccactcag catgataaga gtggagagtg gcaagaaaca agtggagaaa
     3721 tacagtgggt atcaactgaa aagactgatg gtgcagagga gaagcagaag aaggaggagg
     3781 aggatgaaga acttgacctc aataaagccc ttcagcatgc tggcaaacac atggaggatt
     3841 gcatcgtagc ctcctacaca gccctgcttc ttgggtgtct ctgccaggaa agtccaatca
     3901 atgtaactac agtaagggaa tatcttccag aaggagattt ctccataatg acagagatgc
     3961 ttaaaaagtt cttaagcttc atgaatctta cgtgtgctgt tggaacaaca ggccagaagt
     4021 ctatctctag agtgattgaa tatttggaac attgctagct gctttacctt tgcttcaggt
     4081 gcttggtaat gctgaagcta tccttagaca aagaaaagac atgaaagaag tccttgaaga
     4141 tataccaaga acattcatca gtatcattca ttcgtgtttg gatttttatg atcacccgat
     4201 ttcttcatca tgcattctgc gtttgctaaa tgacagttac tacatcaatc tgcagctatc
     4261 aaaaatgagg gaaaaggttc aggctgttaa caatcccatg cagtatttaa atacacttac
     4321 ttggcagagt ctatacctgc ccctgtttcc ttgctgtatt tcaggcaagg tttaaagagg
     4381 gcaatttgtg ctgctgttag tgcaactgct gtgtatgttg agccactgtt gtcatgccag
     4441 tcaggtgcaa aggcagctta gctactgagg tattgaatgt tctgaggaca ttctagacaa
     4501 cagcttagat cctttttcag gctcatttgc ttttgctttt tgttgaatga ttccaatcgt
     4561 aaataaagct tttaataatt ttgtgaattt tttggttgtt gttccctgaa ctactgtcta
     4621 tatttaaaat gagatggaat ccaaagacac aagggattaa tagtatattt ttttattctt
     4681 gattaggttt gggtgttgaa ctatttttca cttctgagac catgaccata ttcaatgtca
     4741 taccattatg tgtcatagct ataggcacaa gaaaacaaat agcagtttga gggaatatta
     4801 tgttatataa gataatgtgc cctgttaaag gattaagcaa aaatagacaa acccagggta
     4861 gtttacactt aatgctgggg aggctcttga aacattgtta cgttttgagg gacgttctct
     4921 agatatattt tctaatgttc agttcagtaa atataataag gaagctaaaa caccaatgtg
     4981 gaatccgttt ttccagatag cgtgtatatt cttctataga gtgacaggat caattgcata
     5041 agcgcaaagc cttaaaattg ctggtgtaga gaagaccctt ttttattcag attctatgtt
     5101 catagagcaa ttgtttagaa aatggttgtg gaacacacag aacatggttt atagggtaaa
     5161 tttcatagat tgttttttct tttttttttt ttcttttttt tttttgtatt gttcagacca
     5221 ctggttatac attttctcct ttttttatta attggcctga ggacaggctt tccagagatg
     5281 tttcattaat acttttaatt acctttcaga tagttacatc atgtttcttc attggatttg
     5341 taaaacttaa agccataaaa atattagttt ggtgtgtatt ggggaaaata gctaaaagtc
     5401 taactttgac tcattttaga ctttgttatt tccttgtata aagtaacaaa ccggggctct
     5461 tgtatccgtg ccagctgtaa tgtttttaaa tgcagtggct gccttctatt gtcttcctat
     5521 ttttgataat gcagattgtt gggaaatcta taaggaagta actgactcta ggcaaattgt
     5581 tttcttcctc ctacccgccc aaccccctac ccgtccgtca ccttttagga acacagtatg
     5641 ccagtgtaat gtgggaacca ttgagactgt atttgccctg agtaccaaac taatttagtg
     5701 aatttttttt taaacatact ggtaaatgat gtccacaaat ttccttagat atattttaat
     5761 taattgtctt aaaatacatt gatatttatt aatcattgga tttggggaca gatttttgac
     5821 tgtggcagat ggtagggaac agaacagtaa acagttggat gagatcaatc agggcgaatt
     5881 acatttttct gttacactgg tgatcatttg agaattgatt tacctcagtg tttaacagtt
     5941 tttgttttgt ttttaaaata ctaactaatt gtcaagcact gatgagatgc aggtttggtg
     6001 gggtgggctg tgaggaagac ctcaccttac caactgcagt gcgtctctgt gttttagcct
     6061 cagagaactc tcattctagg gtacttgagg ctgacttgca aagtgactaa agttttaagg
     6121 taaccttttt tccattgtaa atacttctgt aaatactacc agttggatat tagaacagta
     6181 ggatactttt ctgaatccaa tcctattttt attttataca gtatttctca gctgtgatct
     6241 ttggagcaaa agccaacggc aggaaaaaat agtttgtacc agtttcatga agtatgtctt
     6301 tgggtttttg taaataattt taactcaaat aaaattgata ctttaaatac aaaaaaaaaa
     6361 aaaaaaaaaa a
//
