LOCUS       NM_177033               3578 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus von Willebrand factor C domain containing 2 (Vwc2),
            mRNA.
ACCESSION   NM_177033
VERSION     NM_177033.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Boudkkazi,S., Schwenk,J., Nakaya,N., Brechet,A., Kollewe,A.,
            Harada,H., Bildl,W., Kulik,A., Dong,L., Sultana,A., Zolles,G.,
            Schulte,U., Tomarev,S. and Fakler,B.
  TITLE     A Noelin-organized extracellular network of proteins required for
            constitutive and context-dependent anchoring of AMPA-receptors
  JOURNAL   Neuron 111 (16), 2544-2556 (2023)
   PUBMED   37591201
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Sampath Kumar,A., Tian,L., Bolondi,A., Hernandez,A.A., Stickels,R.,
            Kretzmer,H., Murray,E., Wittler,L., Walther,M., Barakat,G.,
            Haut,L., Elkabetz,Y., Macosko,E.Z., Guignard,L., Chen,F. and
            Meissner,A.
  TITLE     Spatiotemporal transcriptomic maps of whole mouse embryos at the
            onset of organogenesis
  JOURNAL   Nat Genet 55 (7), 1176-1185 (2023)
   PUBMED   37414952
REFERENCE   3  (bases 1 to 3578)
  AUTHORS   Almehmadi,A., Ohyama,Y., Kaku,M., Alamoudi,A., Husein,D.,
            Katafuchi,M., Mishina,Y. and Mochida,Y.
  TITLE     VWC2 Increases Bone Formation Through Inhibiting Activin Signaling
  JOURNAL   Calcif Tissue Int 103 (6), 663-674 (2018)
   PUBMED   30074079
  REMARK    GeneRIF: Our data indicate that VWC2, a bone matrix-associated
            secretory protein, enhances cranial bone formation through
            inhibiting Activin signaling and activin-induced cell functions via
            direct binding to activin A
REFERENCE   4  (bases 1 to 3578)
  AUTHORS   Machado,C.B., Kanning,K.C., Kreis,P., Stevenson,D., Crossley,M.,
            Nowak,M., Iacovino,M., Kyba,M., Chambers,D., Blanc,E. and
            Lieberam,I.
  TITLE     Reconstruction of phrenic neuron identity in embryonic stem
            cell-derived motor neurons
  JOURNAL   Development 141 (4), 784-794 (2014)
   PUBMED   24496616
  REMARK    Erratum:[Development. 2014 Mar 15;141(6):1416]
REFERENCE   5  (bases 1 to 3578)
  AUTHORS   Schwenk,J., Harmel,N., Brechet,A., Zolles,G., Berkefeld,H.,
            Muller,C.S., Bildl,W., Baehrens,D., Huber,B., Kulik,A., Klocker,N.,
            Schulte,U. and Fakler,B.
  TITLE     High-resolution proteomics unravel architecture and molecular
            diversity of native AMPA receptor complexes
  JOURNAL   Neuron 74 (4), 621-633 (2012)
   PUBMED   22632720
REFERENCE   6  (bases 1 to 3578)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   7  (bases 1 to 3578)
  AUTHORS   Miwa,H., Miyake,A., Kouta,Y., Shimada,A., Yamashita,Y.,
            Nakayama,Y., Yamauchi,H., Konishi,M. and Itoh,N.
  TITLE     A novel neural-specific BMP antagonist, Brorin-like, of the Chordin
            family
  JOURNAL   FEBS Lett 583 (22), 3643-3648 (2009)
   PUBMED   19852960
REFERENCE   8  (bases 1 to 3578)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   9  (bases 1 to 3578)
  AUTHORS   Koike,N., Kassai,Y., Kouta,Y., Miwa,H., Konishi,M. and Itoh,N.
  TITLE     Brorin, a novel secreted bone morphogenetic protein antagonist,
            promotes neurogenesis in mouse neural precursor cells
  JOURNAL   J Biol Chem 282 (21), 15843-15850 (2007)
   PUBMED   17400546
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK141514.1 and AL663071.5.
            
            On Apr 13, 2007 this sequence version replaced NM_177033.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033944.1, AK141514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1905              AK141514.1         2-1906
            1906-3578           AL663071.5         107331-109003
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 6.27 cM"
     gene            1..3578
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="von Willebrand factor C domain containing 2"
                     /db_xref="GeneID:319922"
                     /db_xref="MGI:MGI:2442987"
     exon            1..494
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /inference="alignment:Splign:2.1.0"
     exon            495..1290
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /inference="alignment:Splign:2.1.0"
     CDS             598..1572
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="brorin; brain-specific chordin-like protein; von
                     Willebrand factor C domain-containing protein 2; CR
                     (chordin-like cysteine-rich) domain-containing adhesive
                     protein"
                     /codon_start=1
                     /product="brorin precursor"
                     /protein_id="NP_796007.1"
                     /db_xref="CCDS:CCDS36111.1"
                     /db_xref="GeneID:319922"
                     /db_xref="MGI:MGI:2442987"
                     /translation="MPSSSAMAVGALSSSLLVTCCLMVALCSPSIPLEKLAQAPEQPG
                     QEKREHASRDSPGRVSELGRASRDEGSSARDWKSKGSRALSGREAWSKQKQAWAAQGG
                     SAKAADWQVRPRGDTPQGEPPAAAQEAISLELVPTPELPEEYAYPDYRGKGCVDESGF
                     VYAIGEKFAPGPSACPCLCTEEGPLCAQPECPRLHPRCIHVDNSQCCPQCKEKKNYCE
                     FRGKTYQTLEEFVVSPCERCRCEANGEVLCTVSACPQTECVDPVYEPDQCCPICKNGP
                     NCFAETAVIPAGREVKTDECTICHCTYEEGTWRIERQAMCTRHECRQM"
     exon            1291..1420
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..3578
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1791..1796
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="hexamer: AATAAA"
     polyA_site      1815
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
     regulatory      1881..1886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="hexamer: ATTAAA"
     polyA_site      1907
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="major polyA site"
     regulatory      2210..2215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
                     /note="hexamer: AATAAA"
     polyA_site      2238
                     /gene="Vwc2"
                     /gene_synonym="A930041G11Rik; cradin; G11; PSST739;
                     UNQ739"
ORIGIN      
        1 ggggtgtgag ccctacgcta ggcagctgcg acaggcggcg ggcgtgcggc tctcagccag
       61 cctgaatccc attggccctc gggacgcgct ccgagctgct cctctccccc agggcgcaga
      121 ggctgcccac tggtccgcga gctcggtttg gagaaattct ttgcctggct agcaaggaga
      181 gcaagactgg gctctggcga ctccctagag cacgcttcat ggtggagact cagggaggag
      241 aaacccaggg atgacaggca gaaggcaaca cgggcactga caggactcca agaaggctac
      301 cggccaccag cagccagatg aacaggctgg gtcaaatgct ctagctaggt gcatgcgcct
      361 ggtggagcgc gcaggtcgga ctgtgctctc cgcggtccgc cctgcgctgg tgcccggccg
      421 gtaccaggat gggcactgga gccccagggc cgcgcccgct acagcccggc gtccctcctg
      481 cgtcctgagc tcatggacgg cgactccagg ctggcagcgg cgcgcccccg ggctgtgaat
      541 gcgactcgct catcgtccgc gctccccgcc cgcccgcccg ccgggacgtg gtaggggatg
      601 cctagctcct ctgcgatggc agttggcgcg ctctccagtt ccctcttggt cacctgctgc
      661 ctgatggtgg ctctgtgcag tccgagcatc ccgctggaga agctggccca agctcctgag
      721 caacccggcc aggagaagcg cgagcacgcg tctcgggaca gcccggggcg ggtgagcgag
      781 ctcgggcgcg cctcgaggga cgagggcagc agtgcccggg actggaagag caagggcagc
      841 cgcgcgctct cgggccggga ggcgtggagc aagcagaagc aggcctgggc tgcccagggc
      901 gggagcgcca aggccgcgga ctggcaggtc cggcctcgcg gggatacccc acagggggag
      961 cccccggccg ccgcccagga agctatcagc ctggaactcg ttcccacacc ggagctgcct
     1021 gaggaatacg cctacccgga ctaccgcggg aagggctgtg tggacgagag tggcttcgtg
     1081 tatgcgatcg gggagaagtt cgccccaggt ccctcagcct gcccgtgtct gtgtacggag
     1141 gaggggcccc tttgcgcaca gccagagtgc ccacggcttc acccgcgctg catccacgtc
     1201 gacaacagcc agtgctgtcc gcagtgcaag gagaagaaga actactgcga gttccggggc
     1261 aagacctacc agactttgga ggagtttgtg gtgtctccat gtgaacggtg ccgctgtgaa
     1321 gccaatggtg aagtgctgtg cacagtgtcg gcgtgtcccc agacggaatg tgtggacccc
     1381 gtatatgagc ctgatcagtg ctgcccgatc tgcaaaaacg gtccaaactg ctttgcagaa
     1441 accgctgtaa tcccagctgg tagagaggtg aagactgacg agtgcaccat atgtcactgc
     1501 acttatgaag agggcacgtg gagaattgaa cggcaagcca tgtgcacgag acatgagtgc
     1561 agacaaatgt agacttcaca atacagaaac tctcatgttt ttctagaata ttttactaat
     1621 gtgacattct agatgactct gagaaatatc aatcaaaaac agaagacttt ggataaggag
     1681 tcaaggaaaa tggttggtac ttttgttttt cttggtaaca aatacagcaa gagacagaaa
     1741 tggatgtatt tcaaaacatc aataagaact ttgggcatac tccctctcta aataaatgtg
     1801 ctattttcac agtaagtaca ctaaagttca ctattatata ttaaatgtgt ttctataatc
     1861 cctctattag agtcttatat attaaaaaaa tgctgttgtc aaccgtcctc actgtgtacc
     1921 ggtatatctt tgttagcctt cactgcatca cgaaagggtg ctagtggttc caggaaactc
     1981 acctgcacat ggaatgctct actccacaaa aaactccaga aaataatgag tgctttatat
     2041 caaaatcctg ttcaaatgct atgtatctaa tgcttaaaac acaatctaat ttacacaaac
     2101 agattatttt agtaacccat ttcttttaat agtttattct tagtcatttg taatatggag
     2161 gtataaagat tactaacagg tactttctca gttctcattg cagcccatga ataaacactt
     2221 atgtgcattt cattttcatc catggtacct attcttctaa acatgacatc aattcatttt
     2281 ctgagcaaaa ggaaggctgg gatatctgtg ttagtgtccg tggagctggg atgttataaa
     2341 aaggagggaa agggagtctt tggattctct ggaaaatagt taagaaaagt caaatttctg
     2401 aacaagttta tgcttgaaat ataaaatatg ccacagacat ctcaggaatc agcacagtgt
     2461 tcctatactg ctagttacga tagctatctg tcctcagtag tagacatagt gagatgataa
     2521 atgattgtgc atcaagagca gtgtgagcaa gctattcttt gtccagttat gcctaagcat
     2581 tggaaaataa aggctactaa gactctcctg cagaaatata tcacccaaat tatcttattt
     2641 gaatttaaat gataacactg tcattaggtg gtgctcactg aaggaacagt tccctttctg
     2701 cgagtgacat caacagttgg tacatgagtt agagaggcag tcagggtctt aatatgtgta
     2761 aacaaaacta aagtaactat ttgcaaactt taagaacatt ttcctccatt tatttttttt
     2821 ctaaatttta aagttaagta cctatcacac aacaaatctt gtgaagacaa agggaaatta
     2881 gggctggttt gttttcttca tgagtatctg tttctgtagc ataacaggac tataaaatca
     2941 caatgtatgt gattatatat aaatcaaatt agaggctaga aaatgaagat ttctaaaaag
     3001 cacaatttat acccccaaac cctgccagta tacaagaatt tcagtaatta ttgaatgaca
     3061 gccaaggaaa atcaatgtat tttcatcatt aaaatgttaa tcattatata ccttaacaaa
     3121 tacaaaaatt accaatacat ttgcatatgc ttatatttta ccttgattct gaagaatatt
     3181 ttgggtagca cagcataggc agagtagcat gtacatcaat ttcatgaaaa tactcagggg
     3241 tattattggc tagttactct caggtacctg ttttcagctt tccaacactt aaatgttagt
     3301 atgaaagcct gttaggcacc atctgtcctt ccattactca acagtagatt tcctttaaaa
     3361 caaaggtcat ctaaggttac catcttgcca acaaatgact tatcctccta acatacatgg
     3421 gctattgagc cagttgtaat tacatacaat ctgcctcttt tctccacata agacatggtg
     3481 aattacaatt gcagtgtatg aggcccaatt ttcttatttt ccttggcaat agaattccta
     3541 cagaatggaa aaatgggctc tcagattatt gcagtggg
//
