LOCUS       NM_178791               2745 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus V-set and transmembrane domain containing 4 (Vstm4),
            transcript variant 1, mRNA.
ACCESSION   NM_178791
VERSION     NM_178791.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Shi,L., Zhao,M., Abbey,C.A., Tsai,S.H., Xie,W., Pham,D.,
            Chapman,S., Bayless,K.J., Hein,T.W., Rosa,R.H. Jr., Ko,M.L., Kuo,L.
            and Ko,G.Y.
  TITLE     Newly Identified Peptide, Peptide Lv, Promotes Pathological
            Angiogenesis
  JOURNAL   J Am Heart Assoc 8 (22), e013673 (2019)
   PUBMED   31698979
REFERENCE   3  (bases 1 to 2745)
  AUTHORS   Shi,L., Ko,S., Ko,M.L., Kim,A.J. and Ko,G.Y.
  TITLE     Peptide Lv augments L-type voltage-gated calcium channels through
            vascular endothelial growth factor receptor 2 (VEGFR2) signaling
  JOURNAL   Biochim Biophys Acta 1853 (5), 1154-1164 (2015)
   PUBMED   25698653
  REMARK    GeneRIF: Vascular endothelial growth factor receptor 2 (VEGFR2)
            interacted with peptide Lv.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133585.11.
            
            On Mar 29, 2018 this sequence version replaced NM_178791.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053685.1, AK033244.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC133585.11        68281-68491
            212-610             AC133585.11        75058-75456
            611-679             AC133585.11        98479-98547
            680-787             AC133585.11        104151-104258
            788-821             AC133585.11        116088-116121
            822-928             AC133585.11        129360-129466
            929-990             AC133585.11        130759-130820
            991-2745            AC133585.11        149262-151016
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.4 cM"
     gene            1..2745
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /note="V-set and transmembrane domain containing 4"
                     /db_xref="GeneID:320736"
                     /db_xref="MGI:MGI:2444633"
     exon            1..211
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..1116
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; V-set and transmembrane domain-containing
                     protein 4"
                     /codon_start=1
                     /product="V-set and transmembrane domain-containing
                     protein 4 isoform 1 precursor"
                     /protein_id="NP_848906.1"
                     /db_xref="CCDS:CCDS26923.1"
                     /db_xref="GeneID:320736"
                     /db_xref="MGI:MGI:2444633"
                     /translation="MRLRLLALAAAVLLGPAPEVCGALNVTVSPGPVVDYLEGENATL
                     LCHVSQKRRKDSLLAVRWFFAPDGSQEALMVKMTKLRIIQYYGNFSRTANQQRLRLLE
                     ERRGVLYRLSVLTLRPTDQGQYVCKVQEISKHRNKWTAWSNGSSATEMRVISLKAGED
                     SSFEKKKVTWAFFEDLYVYAVLVCCVGILSVLLFTLVIAWQSVFHKRKSRVRHYLVKC
                     PQNSSGETVTSVTSLAPLQPQKGKRQKKKVDVPPAVPAKAPIATTFHKPKLLKPQRKV
                     ALPKITEENLTYAELELIKPHRAAKGVPTSTVYAQILFEENQL"
     exon            212..610
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            611..679
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            680..787
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            788..821
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            822..928
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            929..990
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            991..2745
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2237..2242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /note="hexamer: GATAAA"
     polyA_site      2260
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /note="major polyA site"
     regulatory      2724..2729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2745
                     /gene="Vstm4"
                     /gene_synonym="E130203B14Rik"
ORIGIN      
        1 cagttagtgg atggagaggc cgctcctcca gctcggcgta gacccagaga cccagcagcg
       61 ctggcccgaa ctgtggctgg aggccgaggg gcgggccggg cccggcgggc ggcggcgctc
      121 tcccgggtca cagcgccggg cggaggcggc gggaagatgc ggctgcggct cctagcgctg
      181 gcggcggccg tgctgctggg tccggccccg gaagtctgtg gtgctctcaa tgtcacggtg
      241 tccccaggac ctgtggtgga ctacttggaa ggggaaaatg ccaccctcct ctgccacgtc
      301 tcccagaaga gacggaagga cagcttgctg gccgtgcgct ggttcttcgc ccctgacggc
      361 tcccaggagg ccttgatggt gaagatgacg aagctccgga taattcagta ctatgggaac
      421 ttcagccgga ctgccaacca gcagaggcta cgcctgctcg aggagcgtcg aggggtgctg
      481 tacaggctgt ctgtcctgac gctccggccc acagatcaag ggcagtatgt ctgcaaagtg
      541 caagaaatca gcaagcaccg caacaagtgg acagcctggt ccaatggctc ctcggcgacg
      601 gaaatgagag tgatctccct caaagctggc gaagattcat catttgagaa aaagaaggtg
      661 acttgggcat tttttgaaga tctctatgtg tacgctgtcc ttgtgtgctg cgtggggatc
      721 ctcagtgttc tgctcttcac cctggtcatt gcctggcagt ctgtgtttca caagaggaaa
      781 tcaagagtga gacattattt ggtgaagtgc cctcagaaca gctcagggga gactgtcacc
      841 agtgtgacca gcttggcccc actgcagcca cagaagggta agaggcagaa gaagaaggta
      901 gatgttccac ctgcagtccc tgccaaagcg ccgatagcca ccactttcca caaaccaaag
      961 ctgctgaaac cacagaggaa agtcgccctg cccaagatca ccgaggaaaa cttgacctat
     1021 gccgagctgg agctgatcaa accacacagg gctgccaaag gcgtccccac cagcaccgtg
     1081 tatgcacaga tcctcttcga ggagaaccag ctgtagcgat acctcctctc tggctgtcat
     1141 gtgttctccc agttgtttat gacactcaga aacaaactcc ctagttttgt attttcaccc
     1201 gtgccttcag tgtgatgggg agccccttcc cacagcgttc tgatgtcttc taagaggtac
     1261 acacttccca gaagagaagg gaccagctct tggccatgcc tcccaagata agaggcccct
     1321 ggcctgattc tgagcacaag gactctgctt ctgagagcat tgctgagcca accgtaccaa
     1381 cttctcctct tcttaagcct taaagttttg agggaaaaat caaatcttaa ttttaatcag
     1441 ccccccttgt tctgtataac aagcatccag tttatagcca caggaaatgc tgtaaaggtc
     1501 acagagagaa atgggatata tggactgaca agttctctaa ggctctggga cacccaggac
     1561 agagcaaacc cctggaggtg gtggcaggag ccacttatga tgacatccat tgcttttaat
     1621 agatgctctg aaactggcca tgtgagggca gagatgctga tggttgaaag ccggagatct
     1681 ggctcagaaa attctggtct tatgtcttta gagccatact tcaccagggc tgtagatcta
     1741 caagaacacc cttgtaaagt gtttgtcccc ctttagcaga atggacctag agagacatat
     1801 tgttctctct tttccaaaga cttgagtatg gctccagtgg gtacatcggg taagtgagca
     1861 aagcatgcaa gctcagtcaa ctccattcaa gatagagtgg agcctttcac cattccctca
     1921 gcagagaaat gaaagcacaa ggcatgccgg gaaactatgt ccaggaggac tcaacccttg
     1981 gcaagtgctt tgaccgtctc aatcttggat gagaaccatg attgccttgg gtggatgtca
     2041 ggggaccatg ggacataagt ccgtggggaa gtgactctgt ggacctaaac aatgtacaaa
     2101 aatgtcagac ttaatggaag taagagagtc accttgattt ccgcagtgct attgatgctt
     2161 cttgatgtat actctggtgg ccacttacta cactttataa acattgtctg gctttgtaat
     2221 tttacaatgt atatatgata aattatctat tttaaacaca gctagggtgt gcattgtgcc
     2281 ctctgtctca ctgtgggact tgagtttttt attaccttaa cttggatcac agctacacaa
     2341 gttgtgggaa tgggggaacc cactgaacat tgccttttaa tggggaatag aagcctcaca
     2401 gcatccctgc cgagcttgtc tctctgactt tttcaaaggg aaaccacagc agcaccctca
     2461 gagcagggac aatgagcagt ttgtacctgg tgcttgtttg aagtaacata ttttggggtt
     2521 cttgatcaga aaatgtgtct agtcgctctt cctttaccac atagaccact aaccgtgaat
     2581 tgacatttct gaagctaagt gaggagaagc atccatcatc tggaaagtgc aaagggttct
     2641 tcttgcgaca aggcatcaat aggagcggtg atgtaatcac tgagtagttc cccaaatgga
     2701 cagctgcttt catgttccat gcaattaaag caagcatgac ctcaa
//
