LOCUS       NM_001419157            2469 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus vaccinia related kinase 3 (Vrk3), transcript variant
            6, mRNA.
ACCESSION   NM_001419157 XM_006540538
VERSION     NM_001419157.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Eltokhi,A., Santuy,A., Merchan-Perez,A. and Sprengel,R.
  TITLE     Glutamatergic Dysfunction and Synaptic Ultrastructural Alterations
            in Schizophrenia and Autism Spectrum Disorder: Evidence from Human
            and Rodent Studies
  JOURNAL   Int J Mol Sci 22 (1), 59 (2020)
   PUBMED   33374598
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2469)
  AUTHORS   Kang,M.S., Choi,T.Y., Ryu,H.G., Lee,D., Lee,S.H., Choi,S.Y. and
            Kim,K.T.
  TITLE     Autism-like behavior caused by deletion of vaccinia-related kinase
            3 is improved by TrkB stimulation
  JOURNAL   J Exp Med 214 (10), 2947-2966 (2017)
   PUBMED   28899869
REFERENCE   4  (bases 1 to 2469)
  AUTHORS   Park,N., Song,J., Jeong,S., Thi Tran,T., Ko,H.W. and Kim,E.Y.
  TITLE     Vaccinia-related kinase 3 (VRK3) sets the circadian period and
            amplitude by affecting the subcellular localization of clock
            proteins in mammalian cells
  JOURNAL   Biochem Biophys Res Commun 487 (2), 320-326 (2017)
   PUBMED   28412365
  REMARK    GeneRIF: Finally, downregulation of hvrk3 using siRNA reduced the
            amplitude and lengthened the period of the cellular bioluminescence
            rhythm. Taken together, these data suggest that VRK3 plays a role
            in setting the amplitude and period length of circadian rhythms in
            mammalian cells.
REFERENCE   5  (bases 1 to 2469)
  AUTHORS   Song,H., Kim,W., Choi,J.H., Kim,S.H., Lee,D., Park,C.H., Kim,S.,
            Kim,D.Y. and Kim,K.T.
  TITLE     Stress-induced nuclear translocation of CDK5 suppresses neuronal
            death by downregulating ERK activation via VRK3 phosphorylation
  JOURNAL   Sci Rep 6, 28634 (2016)
   PUBMED   27346674
  REMARK    GeneRIF: Stress-induced nuclear translocation of CDK5 suppresses
            neuronal death by downregulating ERK activation via VRK3
            phosphorylation
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2469)
  AUTHORS   Nichols,R.J. and Traktman,P.
  TITLE     Characterization of three paralogous members of the Mammalian
            vaccinia related kinase family
  JOURNAL   J Biol Chem 279 (9), 7934-7946 (2004)
   PUBMED   14645249
REFERENCE   7  (bases 1 to 2469)
  AUTHORS   Vega,F.M., Gonzalo,P., Gaspar,M.L. and Lazo,P.A.
  TITLE     Expression of the VRK (vaccinia-related kinase) gene family of p53
            regulators in murine hematopoietic development
  JOURNAL   FEBS Lett 544 (1-3), 176-180 (2003)
   PUBMED   12782311
  REMARK    GeneRIF: VRK genes play a role during embryonic development of
            hematopoiesis
REFERENCE   8  (bases 1 to 2469)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2469)
  AUTHORS   Angata,T., Kerr,S.C., Greaves,D.R., Varki,N.M., Crocker,P.R. and
            Varki,A.
  TITLE     Cloning and characterization of human Siglec-11. A recently evolved
            signaling molecule that can interact with SHP-1 and SHP-2 and is
            expressed by tissue macrophages, including brain microglia
  JOURNAL   J Biol Chem 277 (27), 24466-24474 (2002)
   PUBMED   11986327
REFERENCE   10 (bases 1 to 2469)
  AUTHORS   Rennie,P.S., Bruchovsky,N., Akakura,K., Goldenberg,S.L., Otal,N.,
            Akakura,S., Wong,P. and Tenniswood,M.
  TITLE     Effect of tumour progression on the androgenic regulation of the
            androgen receptor, TRPM-2 and YPT1 genes in the Shionogi carcinoma
  JOURNAL   J Steroid Biochem Mol Biol 50 (1-2), 31-40 (1994)
   PUBMED   8049130
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC150895.2 and AC158231.2.
            
            On Mar 28, 2023 this sequence version replaced XM_006540538.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13862062.130927.1,
                                           SRR7345562.2398149.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-739               AC150895.2         21149-21887         c
            740-801             AC150895.2         18370-18431         c
            802-941             AC150895.2         16591-16730         c
            942-1091            AC150895.2         12651-12800         c
            1092-1286           AC150895.2         10652-10846         c
            1287-1351           AC150895.2         9604-9668           c
            1352-1418           AC150895.2         7489-7555           c
            1419-1503           AC150895.2         6337-6421           c
            1504-1609           AC150895.2         5658-5763           c
            1610-1702           AC150895.2         3332-3424           c
            1703-1835           AC150895.2         1971-2103           c
            1836-1956           AC150895.2         893-1013            c
            1957-2015           AC158231.2         43563-43621
            2016-2175           AC158231.2         48075-48234
            2176-2469           AC158231.2         49921-50214
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.9 cM"
     gene            1..2469
                     /gene="Vrk3"
                     /note="vaccinia related kinase 3"
                     /db_xref="GeneID:101568"
                     /db_xref="MGI:MGI:2182465"
     exon            1..739
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            740..801
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            802..941
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     CDS             803..2164
                     /gene="Vrk3"
                     /EC_number="2.7.11.22"
                     /note="isoform 1 is encoded by transcript variant 6;
                     serine/threonine-protein kinase VRK3; inactive
                     serine/threonine-protein kinase VRK3;
                     serine/threonine-protein pseudokinase VRK3"
                     /codon_start=1
                     /product="serine/threonine-protein kinase VRK3 isoform 1"
                     /protein_id="NP_001406086.1"
                     /db_xref="GeneID:101568"
                     /db_xref="MGI:MGI:2182465"
                     /translation="MISFCPVCGKSVKVSFKFCPYCGKALPVEEDGGTQSAVTPHVSS
                     VPGSRRDLNSSFETSPKKVKCSHTVTSLPLSRHSDCDSSGSDNTLTSPDRATGTRSRP
                     LTPKGSPLSNRQSPQTLKRTRVTTSLQALATGTELTDQNGKHWTLGALQIRDDQGILY
                     EAEPTSAVPSESRTQKWRFSLKLDSKDGRLFNEQNFFQRVAKPLQVNKWKKQFLLPLL
                     AIPTCIGFGIHQDKYRFLVFPSLGRSLQSALDDNPKHVVSERCVLQVACRLLDALEYL
                     HENEYVHGNLTAENVFVNPEDLSQVTLVGYGFTYRYCPGGKHVAYKEGSRSPHDGDLE
                     FISMDLHKGCGPSRRSDLQTLGYCMLKWLYGSLPWTNCLPNTEKITRQKQKYLDSPER
                     LVGLCGRWNKASETLREYLKVVMALNYEEKPPYATLRNSLEALLQDMRVSPYDPLDLQ
                     MVP"
     exon            942..1091
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1286
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1351
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1418
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1503
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1609
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1702
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1835
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1956
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2015
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2175
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2469
                     /gene="Vrk3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2449..2454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vrk3"
                     /note="hexamer: AATAAA"
     polyA_site      2469
                     /gene="Vrk3"
                     /note="major polyA site"
ORIGIN      
        1 caagtttctg ctccggatcg gagtgagagt ccgggccgtg agaaaccccg gcgcccacgg
       61 ctttttcaca agtacagtga tgaggagccg ctcgcccggc agcctgtgac gtcactgcct
      121 gaggaaggcg gaggccgacc aaggctgggg ttcgggggcg ggagtggggg gagcacccac
      181 ttcctctgtt ttgctgggaa ggtgttgggc ggagcctgtg aggaatgtca gacactccgc
      241 ctccgcctcc acgggtacct cgtcactggt ttctttggga ttgtggggcc cggtccagtc
      301 tctgctactt agggaagtgt tagtcccaca cctgtggaat ttagcctttg gtactctgct
      361 gtgctgcgtt cctgcaacag gagcatagat ggtagcaagc tgatggtcat acgtaggata
      421 aatgaactaa acaaatgggt gcataataaa agccgacttg agtatcagta gtcggaactg
      481 aatgtgcagc cttagacagg agacctcaac actcaagatc cgagcactta ctgtatgcaa
      541 ggatctgtgc aacagacact tattgtatta tatacccgaa ctgccacctc ccctaggagt
      601 agctctaaac tcagcaatgt tctagcactt gggaggctga gtcaggagga tttttgagaa
      661 tagtgaattc caggacagcc caggatggac tacatagtaa tgggcacctc agtttaatta
      721 aagctggatg tacgcttggg ctggtgtcga gatggagtgt ctaacccagt aatccaagga
      781 ccagaccccg aggccacaaa gcatgatctc cttctgtcca gtctgtggca aaagtgtcaa
      841 ggtatcattc aagttctgcc cttactgtgg caaggccctg cctgtagagg aggatggagg
      901 cacccagtct gccgtgacgc ctcatgtgtc atccgttcca ggctccagga gagacctgaa
      961 ctccagtttt gaaacctctc ccaagaaagt gaaatgctca cacactgtca cttctctccc
     1021 actctcccgc cactctgact gtgacagttc tgggtcagac aacaccttga cttctcctga
     1081 cagggccaca ggcacccgga gcagacccct gacccccaaa ggcagcccac tttccaacag
     1141 gcagagccct cagactctga agcggacccg ggtgaccacc tcactccagg ctctagccac
     1201 aggaacagag ctgacagacc agaatgggaa acactggacc ctgggggccc tgcagatccg
     1261 ggatgaccaa ggcattctct atgaagctga gcctacttct gccgtcccct ctgaatcaag
     1321 gacccagaag tggagattct cactcaaact ggactccaag gatgggcgcc ttttcaatga
     1381 gcagaacttc tttcagagag tagccaagcc tctgcaagtg aacaaatgga agaagcagtt
     1441 cttgctcccg ctcctggcca tccccacctg tatcggcttt ggcattcacc aggacaagta
     1501 caggttccta gtattcccca gcctggggag gagccttcag tcagccctgg atgacaaccc
     1561 aaagcatgtg gtatcagaga gatgtgtgct tcaggtggcc tgcaggctgc tggatgctct
     1621 ggagtatctc catgaaaatg agtatgttca cgggaacctg acagctgaga atgtctttgt
     1681 gaatccagag gatctgagcc aggtgaccct ggtgggctat ggcttcacct accgatactg
     1741 cccaggtggc aaacacgtgg cctacaaaga aggcagcagg agtccacacg atggggactt
     1801 ggagttcatt agcatggacc tgcacaaggg atgcggaccc tcccgccgca gcgatctcca
     1861 gaccttgggc tactgtatgc tcaagtggct ttatgggtcc ctgccatgga caaattgcct
     1921 tcccaacacc gaaaagataa ctaggcagaa gcagaagtat ctggacagcc ccgagcgcct
     1981 cgtgggactg tgtggccgct ggaacaaggc ctcagagacc ctgcgggagt acctgaaggt
     2041 ggtgatggcc ctcaattatg aggagaagcc accctatgcc acgctgagga acagcctaga
     2101 agctctgctg caggatatgc gggtgtcacc ctatgaccct ctggacctcc agatggtgcc
     2161 ttagatggaa tccagagctt ccgacttgca gcttgaagta gaacatgaag tagtgtgact
     2221 ggaggcctgt ttgaactcat agctcctaaa agaatccctt gaatgtgcat tctcaccgct
     2281 cccttaggac atatgaatca gcacttgtgt tggggaacct gagtcatgtc atgtaatgtg
     2341 aaactcctcc ctgtctcagc tctggcagct gtggatggag gtaagtggat gctggcggcg
     2401 gcggcggcag cagccactcc actccctatg gcatttctgt gatggcataa taaactgttt
     2461 ttaatcaaa
//
