LOCUS       NM_001364738             508 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus VPS53 GARP complex subunit (Vps53), transcript variant
            3, mRNA.
ACCESSION   NM_001364738
VERSION     NM_001364738.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 508)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 508)
  AUTHORS   Wei,J., Zhang,Y.Y., Luo,J., Wang,J.Q., Zhou,Y.X., Miao,H.H.,
            Shi,X.J., Qu,Y.X., Xu,J., Li,B.L. and Song,B.L.
  TITLE     The GARP Complex Is Involved in Intracellular Cholesterol Transport
            via Targeting NPC2 to Lysosomes
  JOURNAL   Cell Rep 19 (13), 2823-2835 (2017)
   PUBMED   28658628
REFERENCE   4  (bases 1 to 508)
  AUTHORS   Schindler,C., Chen,Y., Pu,J., Guo,X. and Bonifacino,J.S.
  TITLE     EARP is a multisubunit tethering complex involved in endocytic
            recycling
  JOURNAL   Nat Cell Biol 17 (5), 639-650 (2015)
   PUBMED   25799061
REFERENCE   5  (bases 1 to 508)
  AUTHORS   Karlsson,P., Droce,A., Moser,J.M., Cuhlmann,S., Padilla,C.O.,
            Heimann,P., Bartsch,J.W., Fuchtbauer,A., Fuchtbauer,E.M. and
            Schmitt-John,T.
  TITLE     Loss of vps54 function leads to vesicle traffic impairment, protein
            mis-sorting and embryonic lethality
  JOURNAL   Int J Mol Sci 14 (6), 10908-10925 (2013)
   PUBMED   23708095
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 508)
  AUTHORS   Gan,Y., Zhao,X., Hu,J., Wang,Z.G. and Zhao,X.T.
  TITLE     HCCS1 overexpression induces apoptosis via cathepsin D and
            intracellular calcium, and HCCS1 disruption in mice causes
            placental abnormality
  JOURNAL   Cell Death Differ 15 (9), 1481-1490 (2008)
   PUBMED   18511934
  REMARK    GeneRIF: Disruption of HCCS1 in mice leads to embryonic lethality,
            accompanied by abnormal labyrinth architecture resulting from the
            excessive proliferation of trophoblast cells in the placenta.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK009586.1, AK008026.1 and AL591129.24.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK008026.1, BY708014.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AK009586.1         2-27
            27-399              AK008026.1         2-374
            400-508             AL591129.24        131797-131905       c
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.92 cM"
     gene            1..508
                     /gene="Vps53"
                     /gene_synonym="2010002A08Rik; 2310040I21Rik;
                     3100002B05Rik; Hccs1"
                     /note="VPS53 GARP complex subunit"
                     /db_xref="GeneID:68299"
                     /db_xref="MGI:MGI:1915549"
     exon            1..226
                     /gene="Vps53"
                     /gene_synonym="2010002A08Rik; 2310040I21Rik;
                     3100002B05Rik; Hccs1"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..355
                     /gene="Vps53"
                     /gene_synonym="2010002A08Rik; 2310040I21Rik;
                     3100002B05Rik; Hccs1"
                     /note="isoform 2 is encoded by transcript variant 3;
                     vacuolar protein sorting-associated protein 53 homolog"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 53
                     homolog isoform 2"
                     /protein_id="NP_001351667.1"
                     /db_xref="GeneID:68299"
                     /db_xref="MGI:MGI:1915549"
                     /translation="MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNA
                     VEYINTLFPTEQVTNDLNLFLRTHLFP"
     exon            227..307
                     /gene="Vps53"
                     /gene_synonym="2010002A08Rik; 2310040I21Rik;
                     3100002B05Rik; Hccs1"
                     /inference="alignment:Splign:2.1.0"
     exon            308..508
                     /gene="Vps53"
                     /gene_synonym="2010002A08Rik; 2310040I21Rik;
                     3100002B05Rik; Hccs1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agttctccgg aagtgccagg ctagccgagt aagtagcggc tccctcgagc cccgtacctg
       61 gaggcttgct cttctggggt cctggtgaca gacaggtcgc gagcaggatt ggagagggcg
      121 tcgggggctg agcggcggga tgatggagga ggaggaactg gagtttgtgg aggagctgga
      181 ggctgtgctg cagctcacgc cagaggtgca actcgccatc gagcaggtat tcccaagcca
      241 ggatcctctt gatcaagctg acttcaatgc cgttgagtat atcaatactc tgttcccaac
      301 agagcaagtg acaaatgacc tcaatctgtt cttaagaaca catctatttc catagaagaa
      361 tagagtgtaa cactcacaac acgtacactt tcttgagaac atgggattat tttaccaagg
      421 atctgaattt tctctgttcg gcatgaatta ttcaaaagaa ggaaatgatt ttcaataaag
      481 catttagcag tacaatccta ttacaacc
//
