LOCUS       NM_024260               3780 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Mus musculus VPS50 EARP/GARPII complex subunit (Vps50), transcript
            variant 1, mRNA.
ACCESSION   NM_024260
VERSION     NM_024260.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ahumada-Marchant,C., Ancaten-Gonzalez,C., Haensgen,H., Brauer,B.,
            Merino-Veliz,N., Droste,R., Arancibia,F., Horvitz,H.R.,
            Constantine-Paton,M., Arriagada,G., Chavez,A.E. and Bustos,F.J.
  TITLE     Deletion of VPS50 protein in mouse brain impairs synaptic function
            and behavior
  JOURNAL   BMC Biol 22 (1), 142 (2024)
   PUBMED   38926759
  REMARK    GeneRIF: Deletion of VPS50 protein in mouse brain impairs synaptic
            function and behavior.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Paquin,N., Murata,Y., Froehlich,A., Omura,D.T., Ailion,M.,
            Pender,C.L., Constantine-Paton,M. and Horvitz,H.R.
  TITLE     The Conserved VPS-50 Protein Functions in Dense-Core Vesicle
            Maturation and Acidification and Controls Animal Behavior
  JOURNAL   Curr Biol 26 (7), 862-871 (2016)
   PUBMED   26948874
  REMARK    GeneRIF: An evolutionarily conserved C. elegans protein, VPS-50,
            that is required for animals to assume the well-fed behavioral
            state. Both VPS-50 and its murine homolog mVPS50 are expressed in
            neurons, are associated with synaptic and dense-core vesicles, and
            control vesicle acidification and hence synaptic function, likely
            through regulation of the assembly of the V-ATPase complex.
REFERENCE   3  (bases 1 to 3780)
  AUTHORS   Schindler,C., Chen,Y., Pu,J., Guo,X. and Bonifacino,J.S.
  TITLE     EARP is a multisubunit tethering complex involved in endocytic
            recycling
  JOURNAL   Nat Cell Biol 17 (5), 639-650 (2015)
   PUBMED   25799061
REFERENCE   4  (bases 1 to 3780)
  AUTHORS   Tkatchenko,T.V., Moreno-Rodriguez,R.A., Conway,S.J.,
            Molkentin,J.D., Markwald,R.R. and Tkatchenko,A.V.
  TITLE     Lack of periostin leads to suppression of Notch1 signaling and
            calcific aortic valve disease
  JOURNAL   Physiol Genomics 39 (3), 160-168 (2009)
   PUBMED   19723774
REFERENCE   5  (bases 1 to 3780)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   6  (bases 1 to 3780)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY100563.1, AK129458.1 and AI551221.1.
            
            On Nov 17, 2009 this sequence version replaced NM_024260.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK075898.1, SRR1660821.43384.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                BY100563.1         1-50
            51-3554             AK129458.1         1-3504
            3555-3780           AI551221.1         1-226               c
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 1.81 cM"
     gene            1..3780
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /note="VPS50 EARP/GARPII complex subunit"
                     /db_xref="GeneID:73288"
                     /db_xref="MGI:MGI:1920538"
     exon            1..163
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..3025
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coiled-coil domain-containing protein 132; coiled-coil
                     domain containing 132; EARP/GARPII complex subunit VPS50;
                     bcl2-like protein blm; syndetin"
                     /codon_start=1
                     /product="syndetin isoform 1"
                     /protein_id="NP_077222.4"
                     /db_xref="CCDS:CCDS39415.1"
                     /db_xref="GeneID:73288"
                     /db_xref="MGI:MGI:1920538"
                     /translation="MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQP
                     SDPQAEQELINSIEQVYFSADPFDIVKYELEKLPPVLNLQELEEYRDKLKQQQSAVSK
                     KVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLAN
                     QRKRQLLIGLLKSLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSC
                     ISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMH
                     FTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWE
                     VMLSYYRTMEWHEKHDNEETAAAAEGSNVMSTEEATFDRGYVKKKLEHGLTRIWQDVQ
                     LKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSVNYFK
                     NHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSS
                     KPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEE
                     LKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQM
                     MNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLKTTLNRIQES
                     LIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLA
                     EQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLM
                     MNVKWDVKEIMSQHNIYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLAN
                     RTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTE
                     NDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDEIDRPKR"
     exon            164..232
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            233..355
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            356..427
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            428..481
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            482..552
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            553..670
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            671..706
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            707..789
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            790..832
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            833..931
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1072
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1205
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1297
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1392
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1491
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1582
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1759
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1878
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1985
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2107
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2188
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2337
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2434
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2593
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2715
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2905
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..3777
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3538..3543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /note="hexamer: AATAAA"
     polyA_site      3561
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /note="major polyA site"
     regulatory      3751..3756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
                     /note="hexamer: TATAAA"
     polyA_site      3777
                     /gene="Vps50"
                     /gene_synonym="1700034M03Rik; 8430415E05Rik; BLM; Ccdc132;
                     mKIAA1861"
ORIGIN      
        1 gaaggctgac ccaggcgggc agctccacgt gacgactcac tatggcttcc ctgtgttgta
       61 gccggctgtc tggtgggatt atgtgatttg ttacttctcc gaggcagggt aattgtctct
      121 cgacttcgac atgcaaaaaa tcaaatctct catgacccgg cagggtctga agagccctcc
      181 ggagagcctc aatgatcttg gtgcctttga gagccttcgg gtccctggaa aggaggaatt
      241 cagggagctt cgagaacagc caagcgaccc tcaagctgag caagagttga ttaatagcat
      301 tgaacaagtc tatttttctg cggatccatt tgatattgtt aaatatgagc tggagaagct
      361 tcctcctgtt ctcaatctgc aagagttaga ggagtacaga gacaaattaa agcaacaaca
      421 atctgcagtc tctaaaaaag tggcagattt gattcttgaa aagcagcctg cttatgtaaa
      481 ggaacttgaa agagtcacat cactgcagac aggtcttcag ttggctgctg tcatctgcac
      541 aaatgggagg aggcacttga atattgcaaa ggaaggtttt actcaggcta gcttaggcct
      601 tcttgcaaat caaaggaagc gtcagttgct aattggactt ctgaagtcgc tgagaaccat
      661 aaaaacattg caaagaactg acatacgctt aagtgaaatg ctggaggaag aggactatcc
      721 aggagctatt cagctgtgcc tagaatgtca gaaggctgcc agcactttta aacactacag
      781 ctgtataagt gagctgaatt caaagctgca agatactttg gagcaaattg aggaacagtt
      841 ggatgtagct ctttccaaaa tctgcaagaa ttttgacatt aaccattata ccaaggttca
      901 gcaagcttac cggcttcttg gaaaaacaca gactgcgatg gatcagcttc atatgcactt
      961 cactcaagcc attcacaaca ccgtatttca ggttgtgctt ggctatgtgg agctgtgtgc
     1021 aggaaacacg gatacaaaat tccagaagct tcagtataaa gatctctgta cgcatgtgac
     1081 accagacagc tatattccat gcctggcaga cctgtgcaaa gcactgtggg aagtcatgct
     1141 cagctactac agaaccatgg agtggcacga gaaacatgac aatgaggaaa cagctgcagc
     1201 tgctgaagga agcaatgtga tgagtactga ggaggccact tttgaccgtg gctatgtaaa
     1261 gaagaagtta gagcacggcc ttacccggat atggcaggat gttcaactaa aagtaaaaac
     1321 ctacttgctt ggaactgatt tatctatatt caaatatgat gatttcatct ttgttctgga
     1381 tattgtcagc aggttgatgc aagttggaga agaattttgt ggtagcaagt ctgaagtttt
     1441 gcaggagtcc attagaaaac aaagtgtcaa ttacttcaag aaccaccata ggatacgact
     1501 tgatgaactg agaatgttct tagagaatga gacatgggag ctttgccctg ttaagtcaaa
     1561 ttttagcatc ttgcagcttc atgagtttaa attcctggaa cagtctcgct ccccatcagt
     1621 ttcacctagt aagcagccat cagcaacttc ttcaaagcct gtgactttgt ttgagcagta
     1681 ctgcagtggt gggaatccat ttgaaataca agccgaccac aaagatgaag aaactgaaga
     1741 tgtcttagcc tctaatgggt atgagtctga tgagcaagag aagagcgcct atcaggacta
     1801 tgacagtgac agcgacgtcc ctgaggagct gaagcgggat tacgtggacg agcagaccgg
     1861 cgacgtgccc gtgaagagtg tttcccgaga gaccctgaag agcaggaaga agtcggatta
     1921 cagtctaaac aaagtgaatg cacccatctt aacaaacaca acactgaatg taataaggct
     1981 tgttggaaag tacatgcaga tgatgaacat ccttaagcca atcgccttcg atgtgatcca
     2041 tttcatgtct cagctctttg actattactt gtatgccata tatacctttt ttggtcggaa
     2101 tgattcattg gaatcaacag gacttggcct tagtagtagc agactaaaaa caactctaaa
     2161 cagaattcaa gaaagcctta ttgacctgga gggctcagct gatccaactg ccactctcac
     2221 agcagccgag gaaagaaagg agaaggtacc cagcccacac ctcaatcagc tagtgatttt
     2281 gacatctggg gatacattgt atgggttggc agagagagtg gtagccacag aatccttagt
     2341 gttcttggct gagcagtttg agttccttca gccacatttg gatgctgtga tgcctgcagt
     2401 caaaaagccc ttcctgcagc agttttactc ccagacagtc tcaactgcca gtgaactgcg
     2461 caagcccatt tactggattg tggctggtaa agctattgat tatgaacaga tgcttctcct
     2521 aatgatgaat gtgaagtggg atgtcaaaga gatcatgtca cagcacaaca tctatgtaga
     2581 tgcattgctg aaggaatttg agcagtttaa caagcggtta aatgaagttt ctaagagagt
     2641 tcggatacct ctgcctgtat caaatatact ttgggagcat tgtatacgat tagctaacag
     2701 aactattgtg gaaggatacg ccaatgtcaa gaaatgcagt aatgaggggc gtgccttgat
     2761 gcaattggat tttcaacagt ttttaatgaa actcgaaaaa ctaactgaca ttagacctat
     2821 tcctgataaa gaatttgtgg aaacgtatat caaagcctac tacctaactg agaatgacat
     2881 ggagcgttgg atcaaagagc acagggaata ctcaacgaag cagttgacca acctggtgaa
     2941 tgtttgccta ggatctcata tcaataagaa agcaagacag aagctcctag cagctataga
     3001 tgagatagac aggcctaaaa gataatgaac agagctcttt cctcggggac attgatcttc
     3061 tttcggcctg tacgacatga aaggcagttc ttcatacact cctcataatt ctcttctaag
     3121 aaaactttga gcatgttctt tggaactgga aaatagaaaa tactcaactg tgtgtggtgt
     3181 tctcaagtga cttttatatg ctccagtttc ttcaaatttt gctcttgttg taaccttttc
     3241 ctgaaattat cttactctca taattattat gtgactacaa ttacacatgt tgcaaaagag
     3301 tgtactataa atacatagtt ggaagggata gtggctaatg tgtctatgaa tagtttttgg
     3361 agagtgtctt aaatgggagg atgaatgtac agtatttcct aattatgagc attgcatgag
     3421 aatttttaaa aaatgtaagt aaaatagttg gaaaatcaat atgttctctg tttttaactg
     3481 tcagagcttc tgccagtgtt aatttggcta taacagtgat cataaatgat gaaatttaat
     3541 aaatgttaca ctctaaaatt accaggtgtg tgagactact accttttgat attcctgtgt
     3601 gatgggaaga cttcaggaga cagtagtgtc attttttatt atgtcctcac ccttgttctc
     3661 tatcttgaaa cgtctggtcg gatgacattt tattgtatgg attttccagt gcctgagtag
     3721 catattgaac aattactaac tatgcatttt tataaaaata taaaatattt tacaggaaaa
//
