LOCUS       NM_027338               3660 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus vacuolar protein sorting 36 (Vps36), transcript
            variant 1, mRNA.
ACCESSION   NM_027338
VERSION     NM_027338.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Hermosilla Aguayo,V., Martin,P., Tian,N., Zheng,J., Aho,R., Losa,M.
            and Selleri,L.
  TITLE     ESCRT-dependent control of craniofacial morphogenesis with
            concomitant perturbation of NOTCH signaling
  JOURNAL   Dev Biol 503, 25-42 (2023)
   PUBMED   37573008
REFERENCE   3  (bases 1 to 3660)
  AUTHORS   Smith,J., Liu,F., Beyer,B., Morales,K., Reilly,A., Cole,R. and
            Herron,B.J.
  TITLE     Angiogenesis QTL on Mouse Chromosome 8 Colocalizes with
            Differential beta-Defensin Expression
  JOURNAL   J Biomol Tech 26 (2), 45-53 (2015)
   PUBMED   25802489
REFERENCE   4  (bases 1 to 3660)
  AUTHORS   Hirano,S., Suzuki,N., Slagsvold,T., Kawasaki,M., Trambaiolo,D.,
            Kato,R., Stenmark,H. and Wakatsuki,S.
  TITLE     Structural basis of ubiquitin recognition by mammalian Eap45 GLUE
            domain
  JOURNAL   Nat Struct Mol Biol 13 (11), 1031-1032 (2006)
   PUBMED   17057714
  REMARK    GeneRIF: presentation of the complex structure of Eap45 GLUE and
            ubiquitin at 3.35 A resolution, which explains the previously
            uncharacterized mechanism of Ub recognition by mammalian ESCRT-II
REFERENCE   5  (bases 1 to 3660)
  AUTHORS   Slagsvold,T., Aasland,R., Hirano,S., Bache,K.G., Raiborg,C.,
            Trambaiolo,D., Wakatsuki,S. and Stenmark,H.
  TITLE     Eap45 in mammalian ESCRT-II binds ubiquitin via a
            phosphoinositide-interacting GLUE domain
  JOURNAL   J Biol Chem 280 (20), 19600-19606 (2005)
   PUBMED   15755741
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117665.6.
            
            On Feb 9, 2019 this sequence version replaced NM_027338.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156241.1, AK161865.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC117665.6         160196-160329
            135-203             AC117665.6         166261-166329
            204-274             AC117665.6         169637-169707
            275-389             AC117665.6         173030-173144
            390-479             AC117665.6         174140-174229
            480-566             AC117665.6         175220-175306
            567-599             AC117665.6         177632-177664
            600-677             AC117665.6         177752-177829
            678-812             AC117665.6         178901-179035
            813-878             AC117665.6         180858-180923
            879-943             AC117665.6         183875-183939
            944-1028            AC117665.6         184488-184572
            1029-1105           AC117665.6         185575-185651
            1106-3660           AC117665.6         185762-188316
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 11.14 cM"
     gene            1..3660
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="vacuolar protein sorting 36"
                     /db_xref="GeneID:70160"
                     /db_xref="MGI:MGI:1917410"
     exon            1..134
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..1199
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein-sorting-associated protein 36; ESCRT-II
                     complex subunit VPS36"
                     /codon_start=1
                     /product="vacuolar protein-sorting-associated protein 36
                     isoform 1"
                     /protein_id="NP_081614.1"
                     /db_xref="CCDS:CCDS22171.1"
                     /db_xref="GeneID:70160"
                     /db_xref="MGI:MGI:1917410"
                     /translation="MDRFVWTSGLLEINETLVIQQRGVRVYDGEEKIKFDAGTLLLST
                     HRLIWRDQKNNECCMAIPLSQIVFIEEQAAGIGKSAKIVVHLHPAPSNKEPGPFQSSK
                     NSYIRLSFKEHGQIEFYRRLSEEMTQRRWETVPVSQSLQTNKGPQPGRVRAVGIVGIE
                     RKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKEKQGDVTEDETIRFK
                     SYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQAPLEERGGIMSLTEVYCLVNRA
                     RGMELLSPEDLVNACKMLEALKLPIRLRVFDSGVMVIELQTHKEEEMVASALETVSER
                     GSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQN"
     exon            135..203
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            204..274
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            275..389
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            390..479
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            480..566
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            567..599
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            600..677
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            678..812
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            813..878
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            879..943
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1028
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1105
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..3660
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1282..1287
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="hexamer: AATAAA"
     polyA_site      1304
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="major polyA site"
     regulatory      1318..1323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="hexamer: TATAAA"
     polyA_site      1343
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
     regulatory      3524..3529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="hexamer: GATAAA"
     polyA_site      3545
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="major polyA site"
     polyA_site      3578
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
     polyA_site      3602
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
     regulatory      3637..3642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
                     /note="hexamer: AACAAG"
     polyA_site      3660
                     /gene="Vps36"
                     /gene_synonym="1700010A24Rik; 2210415M20Rik;
                     2810408E15Rik; Eap45"
ORIGIN      
        1 agcaccggcg agcggcagaa gccggccggg tggcggccat ggaccgcttc gtgtggacca
       61 gcggcctcct ggagatcaac gagaccctgg ttatccagca gcgcggggtg cgcgtctacg
      121 acggcgagga gaagataaaa tttgatgccg ggactcttct tcttagtaca caccggctga
      181 tttggagaga ccagaagaat aatgagtgct gcatggccat tcccctgtct cagattgtgt
      241 tcatcgagga gcaggcagct ggaatcggga agagcgccaa aatcgtggtt cacctgcacc
      301 cagctccttc taacaaagag cccggtccgt tccagagcag taagaactcc tacatcagac
      361 tctccttcaa agaacatggc cagattgagt tttacaggcg tttatcagaa gaaatgacac
      421 agagaagatg ggagactgtg ccagtttccc agtcactaca aacaaataaa ggaccccagc
      481 caggaagagt aagagctgtt ggaattgtag gtattgaaag gaaacttgaa gaaaaaagaa
      541 aagaaaccga taaaaacatt tctgaggcct ttgaagacct gagcaagctg atgattaagg
      601 ctaaggaaat ggtggagtta tccaaatcaa tcgctaataa gattaaagag aagcaaggcg
      661 atgtcacaga ggatgagacc atcaggttta agtcgtacct gctgagtatg ggaatagcca
      721 acccagtcac cagagaaacc tatggctccg gcacacagta ccacatgcag ctggccaaac
      781 agctggctgg aatattgcag gcacctttgg aggagcgtgg gggaataatg tccctcacag
      841 aggtgtactg tctagtgaac cgggcccgag gaatggagtt gctctcgcca gaagatttgg
      901 taaatgcgtg caagatgctg gaagcgctaa aattgcctat caggcttcgg gtttttgaca
      961 gtggtgtcat ggtaattgag cttcaaactc acaaggaaga ggaaatggtg gcctcggccc
     1021 tggagacggt ttcagaaagg gggtccttaa catccgaaga gtttgccaag cttgtgggaa
     1081 tgtctgtcct tctggctaaa gaaaggctgc tccttgcaga gaagatgggc cacctttgcc
     1141 gagacgactc cgtggaaggc ttgcggtttt atccaaattt atttatgaca cagaactaag
     1201 aattttgtac ctgaaatgct ttatgtcaat aagcttgcat tttgccaagg tctcacgaca
     1261 ctgagctaag agataaaccc aaataaacca agaatgtgtt agaacttctc agtataatat
     1321 aaaactcttc atctctccca aaatatggtc tcagaaactt acttagggta tatacaggaa
     1381 aatacagaaa aatgaatatg aatatgcatg taaagtcatg ctatactgtg tagaactcct
     1441 gggatataac ttgaaacatg gtggatatta atttgatcct gagttcaggt ccttggagga
     1501 agaagatgtg aagttgcatg tttggataga tcaggtaatc attttgccat gactaaacct
     1561 catcaaattg taaatgtttt cctccacatc taatgtgccc atccttggag gcacacatga
     1621 aggaagcaac agctacactg tagtttaaga atcaacagca ctgaaatcaa actgcagaaa
     1681 gccgtaccag gctcttcctt gggggcgttg gaagcagact taggcatgcc tctaccagat
     1741 caaataaaat acccttggaa aaggagaggg ctaggtccag cttacgtgga gagcactcga
     1801 ttgtgatgca gaggccttgg gcttcagacc taccacccac acaccgggga aggagggagc
     1861 aaggacctgt tatgcattta cacctcaggt cctgtttagt ttctaagtga tagtttcatg
     1921 agtggcattc tttaaagcct gcagcagtat cattttgttt aagaaatgtg gattttgaaa
     1981 tggaattaaa gagctgtata gacacacccc gagttgtaat cacaggtcgg gcagtcggaa
     2041 aggtctggtt ctggaagggc atgaagggcg taggacctta ggaactcttc tgcatggaag
     2101 ctaagggtgt gaaataggaa acagaaaggc taacagatct cctttctcgt ctcccaagaa
     2161 gtctcagcct caaccagacg catattccta tcataggcca gcatgtttct ttcttctaca
     2221 ccctacatgt taacggtcgt gtagagaaag cacctgacga taagtattgc tgacaataga
     2281 acctggctag taccacctcc agattgctgg agctttcagt aggttgcctg acttcatatg
     2341 tatagcatgg tgacgttacc tcaaactcag ggagcccata ggccagaagg tgcttttcgg
     2401 aggctgagct gaggtggaag ggtggtgata gcgtggcctt ggagccacag ggagggatgc
     2461 gcaggcacac tatctgggag tttgaggaca ctgtctggat gaggtggagg ctactgcaga
     2521 tgccctgtgg agaagtgcca gtgtcctcag ctgcaagcca gggagcccta ccctgacact
     2581 ttcctggaca gtgatggcct ttgcctctgt cctccagtat ctcgagcccg ctgtggccaa
     2641 ggccttttgt gtgggttttc cagtctgtca ctggttgtgg tgaccttagg tcttccctaa
     2701 tcacctcatt gtttatctca gggttacttg agggttaatt cagtgttggc tacccaaatc
     2761 ttctaaggat tgggtagacc agtgtcagtt tattagtgtg tctcggctct ctgtgggata
     2821 cacgtagtct atcacatggc cctgctgcac acacacacac acacacacac acacacacac
     2881 acacgcaagc acgcacattc ctcttgtcaa actaaggatc ttaaaatcca caggtcactg
     2941 ataatctgtt ttagcacttg gattggttgt aaggtttttg ggttgtagag tccaatggat
     3001 ctagcttgtg gcttcaccac cacaaacata ctgcatctgt ttattcagtg tgacagcaga
     3061 ttaacaaaaa aagagtctga tgaagtggca taaacatact ttggaaaagt cacatggatt
     3121 taagaaagaa agaaagaaag aaagaacatt gtttgggttt gttgctgttg ttggtttgcc
     3181 cttctggcgc ttcttttctt gcccagtgtg ccgctgggtc ctttggtact ggctgtgctt
     3241 aaatacaaac caatcaagcc ccgggcggac aaatgccctg ctcttggtgc tcatcactgt
     3301 aatttttttc ctgactttgc tctcaggagc gtgagtatgt aaatatctga taatgaatag
     3361 tttagtcact gtcaagaaaa ttaatttaca ttgtataact gaagactctt ttcagggtta
     3421 ggtgatttgc attgatgtat atatagtaaa attgctgtaa aattttaaac cgattttctt
     3481 aacttgatgt tctttttaaa ctttctgtaa gatacatgca aatgataaac gttcagtcac
     3541 actaagccaa tgtgggaatt tttctgtttt ataattaagc attgcttatt ggttgtcttt
     3601 gagattttta taatctagtt atctttaaaa atgggaaaca agttacaagc attttcataa
//
