LOCUS       NM_029929               4114 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus VPS33A CORVET/HOPS core subunit (Vps33a), transcript
            variant 1, mRNA.
ACCESSION   NM_029929
VERSION     NM_029929.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Yuan,Y., Liu,T., Huang,X., Chen,Y., Zhang,W., Li,T., Yang,L.,
            Chen,Q., Wang,Y., Wei,A. and Li,W.
  TITLE     A zinc transporter, transmembrane protein 163, is critical for the
            biogenesis of platelet dense granules
  JOURNAL   Blood 137 (13), 1804-1817 (2021)
   PUBMED   33513603
REFERENCE   3  (bases 1 to 4114)
  AUTHORS   Hubert,V., Peschel,A., Langer,B., Groger,M., Rees,A. and Kain,R.
  TITLE     LAMP-2 is required for incorporating syntaxin-17 into
            autophagosomes and for their fusion with lysosomes
  JOURNAL   Biol Open 5 (10), 1516-1529 (2016)
   PUBMED   27628032
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4114)
  AUTHORS   Hao,Z., Wei,L., Feng,Y., Chen,X., Du,W., Ma,J., Zhou,Z., Chen,L.
            and Li,W.
  TITLE     Impaired maturation of large dense-core vesicles in muted-deficient
            adrenal chromaffin cells
  JOURNAL   J Cell Sci 128 (7), 1365-1374 (2015)
   PUBMED   25673877
REFERENCE   5  (bases 1 to 4114)
  AUTHORS   Zhen,Y. and Li,W.
  TITLE     Impairment of autophagosome-lysosome fusion in the buff mutant mice
            with the VPS33A(D251E) mutation
  JOURNAL   Autophagy 11 (9), 1608-1622 (2015)
   PUBMED   26259518
  REMARK    GeneRIF: VPS33A(D251E) mutation plays dual roles by increasing the
            HOPS complex assembly and its association with the autophagic SNARE
            complex, which selectively affects the autophagosome-lysosome
            fusion.
REFERENCE   6  (bases 1 to 4114)
  AUTHORS   Novak,E.K., Hui,S.W. and Swank,R.T.
  TITLE     Platelet storage pool deficiency in mouse pigment mutations
            associated with seven distinct genetic loci
  JOURNAL   Blood 63 (3), 536-544 (1984)
   PUBMED   6696991
REFERENCE   7  (bases 1 to 4114)
  AUTHORS   Peters,J., Povey,S., Jeremiah,S. and De Giorgi,L.
  TITLE     Linkage relationships of peptidase-7, Pep-7, in the mouse
  JOURNAL   Biochem Genet 21 (7-8), 801-807 (1983)
   PUBMED   6626149
REFERENCE   8  (bases 1 to 4114)
  AUTHORS   Hakansson,E.M. and Lundin,L.G.
  TITLE     Effect of a coat color locus on kidney lysosomal glycosidases in
            the house mouse
  JOURNAL   Biochem Genet 15 (1-2), 75-85 (1977)
   PUBMED   849254
REFERENCE   9  (bases 1 to 4114)
  AUTHORS   Womack,J.E., Hawes,N.L., Soares,E.R. and Roderick,T.H.
  TITLE     Mitochondrial malate dehydrogenase (Mor-1) in the mouse: linkage to
            chromosome 5 markers
  JOURNAL   Biochem Genet 13 (9-10), 519-525 (1975)
   PUBMED   1203052
REFERENCE   10 (bases 1 to 4114)
  AUTHORS   Swank,R.T., Paigen,K. and Ganschow,R.E.
  TITLE     Genetic control of glucuronidase induction in mice
  JOURNAL   J Mol Biol 81 (2), 225-243 (1973)
   PUBMED   4777306
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB852750.1, AK041982.1, AK140553.1 and BU703144.1.
            
            On Jul 23, 2009 this sequence version replaced NM_029929.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046417.1, SRR1660815.194875.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 BB852750.1         25-29
            6-2604              AK041982.1         2-2600
            2605-3636           AK140553.1         2594-3625
            3637-4114           BU703144.1         141-618
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 63.03 cM"
     gene            1..4114
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="VPS33A CORVET/HOPS core subunit"
                     /db_xref="GeneID:77573"
                     /db_xref="MGI:MGI:1924823"
     exon            1..136
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1831
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 33A; vacuolar
                     sorting protein 33a; buff; vacuolar protein sorting 33A"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 33A
                     isoform 1"
                     /protein_id="NP_084205.3"
                     /db_xref="CCDS:CCDS19666.1"
                     /db_xref="GeneID:77573"
                     /db_xref="MGI:MGI:1924823"
                     /translation="MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTG
                     PFGLIAQYSLLKEHEVEKMFTLKGSRLPAADVKNIIFLVRPRLELMDIIAENVLSEDR
                     RGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAF
                     KECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMVRMKREFTGSQN
                     SVFPVFDNLLLLDRNVDLLTPLASQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGGG
                     SGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVG
                     EIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVN
                     SYIEDCIAQKHPLIKVLRLVCLQSVCNSGLKQKVLDYYRREILQTYGYEHILTLNNLE
                     KAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRP
                     GWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLVFFLGGVTFAEIAALRFL
                     SQLEDGGTEYVIATTKLMNGNSWIEALMEKPF"
     exon            137..202
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            203..330
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            331..517
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            518..634
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            635..809
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            810..1009
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1136
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1204
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1342
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1480
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1649
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..4114
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2104..2109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="hexamer: AATAAA"
     polyA_site      2127
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
     regulatory      3745..3750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="hexamer: ATTAAA"
     polyA_site      3769
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="major polyA site"
     regulatory      3869..3874
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
                     /note="hexamer: ATTAAA"
     polyA_site      3896
                     /gene="Vps33a"
                     /gene_synonym="3830421M04Rik; bf"
ORIGIN      
        1 gcggcgtctg gggaaatgcg ggtgcccggg caagatggcg gcgcacctgt cgtacgggcg
       61 agtgaacctg aacgtgctgc gcgaggcggt gcgtcgggag ctgcgcgagt tcctggacaa
      121 gtgcgctggg agcaaggcaa tagtgtggga tgagtactta acaggaccat ttggactgat
      181 cgcacagtac tcactactaa aggaacatga agtggaaaaa atgttcacac ttaaaggaag
      241 tcgtctgccc gcagctgatg tcaagaacat catttttttg gtcagaccca ggctagagct
      301 gatggacata attgctgaga atgtactcag tgaggacagg cgtggcccta ccagggattt
      361 ccatattttg tttgtgcctc gccggagcct actgtgtgag cagcggctga aggacctggg
      421 ggttttggga tcctttattc atcgagaaga atacagcttg gatctcattc catttgatgg
      481 ggacctctta tccatggagt cagagggcgc tttcaaagag tgctacctgg aaggcgacca
      541 gaccagcctg taccacgcag ccaaggggct gatgaccctg caggcgctgt atggaactat
      601 cccccagatc tttgggaaag gagagtgtgc tcggcaagtg gccaatatga tggtcaggat
      661 gaagagggag ttcacaggaa gccagaactc agtgttcccc gtgtttgata acctcttgct
      721 gcttgatcgc aatgtggatc tcctgacgcc actcgcctcc cagctcacgt atgaaggcct
      781 catcgatgag atttacggca ttcagaacag ttatgtgaag ttgcctccag agaagtttgc
      841 acccaagaag cagggtggcg gcagtggtgg gaaggacctt cccacggagg ccaagaagct
      901 tcagctcaac tccgcagagg agctctacgc cgagatccga gacaagaact tcaacgctgt
      961 gggctctgtg ctcagcaaga aggcgaagat catctctgca gcgttcgagg agcgacacaa
     1021 cgctaagaca gtcggggaga tcaagcagtt tgtgtcccag ctaccccaca tgcaggcagc
     1081 caggggatcg ctcgccaacc acacctcaat tgcagagtta atcaaagacg tcaccacttc
     1141 tgaagacttc tttgataagt tgacagtgga gcaggagttc atgtccggga tagacactga
     1201 taaggtcaac agttacatcg aggactgcat tgcccaaaag catccactca tcaaggtgct
     1261 gagactggtt tgcctccagt ctgtgtgcaa cagcgggctc aagcagaagg tgctggacta
     1321 ttacagaagg gagatcctcc agacctacgg ctatgagcac atactgacct tgaacaacct
     1381 ggagaaggcc ggcctgctca aggctcagac agggggcaga aacaattacc caacaatccg
     1441 gaaaacactg cgcctctgga tggacgatgt caatgaacaa aaccccacag acatatccta
     1501 tgtgtacagc ggttatgctc cactcagcgt gcgactggct cagctccttt cccggccagg
     1561 ctggcggagc atcgaggagg ttctccgcat tctcccaggg ccccactttg aagaaagaca
     1621 gccactgccc accgggctgc agaagaaacg ccagccagga gaaaacagag tcacactggt
     1681 atttttcctc gggggcgtca cctttgcgga aatagccgcc ctgcgatttt tgtcccagct
     1741 ggaagatgga ggcaccgagt atgtcattgc gaccactaaa ctgatgaatg gaaacagctg
     1801 gatagaagct ctcatggaaa agcctttcta gagtatcgga ggagacgtga caggtcacca
     1861 cacaatgagc tgcctctgtg aacgtgttgc caaaggatgt aaggccagga acaggaggac
     1921 ggaagcagtg ggcgcatgtt ccttcctgct cctctctatg ctcttaatcc taaagaaggg
     1981 acaagcaacc tggatgaagg gatgatgaac tgggcataag agacagaggc aggaggattg
     2041 ctgcaagctt gaggacaacc tgggctccat aatgagtttc aggccaaagc tctcccttcc
     2101 agaaataaag gagatcctgt tttggaattt ttaagtctca agtcttcatt ttctggaaat
     2161 ggctaagact atgggtctga tagccctggc aaccacatga ctgcacagac attaaaagca
     2221 gagctgaggg gacgaattca aaaagagaat cagaattctg accactgatc cacagtggca
     2281 tattctagaa gatgtcccat gcccaggact gtggttttct gttgaattgg aagccagcat
     2341 tgtccagtgt gtgcactttc ctgcaaagca catttgtgtc tccagtctct accgagttat
     2401 tcttaaggcg agctttgttt ccttctgaac aactaagagg ttcgaagggg cttcttggca
     2461 taatgacatt tctgctctct gcgttagcca tgactgtgca ggtctctgcc cagaagggac
     2521 tgatcaagag tgagctgagc gccaggcagc ctcttccagc taaccttcca aaggcacttc
     2581 tgagcgtgca tgttggtaac tcctggactc accagggtag gcgctcctgc cgggacttct
     2641 gttttcctcc atgtcctcca tgggcagttc ttctgttctt tcaatatcat gaagctctgt
     2701 ctccgtggta actgccacac acagctctct agccctctag ctgtactcaa ctctgcacac
     2761 cccctttccc tgaaaatact caggtggtca gtgcacttcg gtcagtgtca gtcacaagag
     2821 acaagcagca ccagtgctca gggagtggag cacggccgga agcagagcag gcatggcctc
     2881 ctgatcatct cagcatctga aggagctaag agcaccaagt gggggaccac gttgatggct
     2941 ttcctgtcat cactcaagac tccagaaaag ctagcctgtc tcagccggtc ctggtccttg
     3001 cccaggtgca gtttctatgg ttaatatttt cttcttgttt ttctcatgta gaatcaggag
     3061 caatacactg cacattataa gtaaccagag ttgggtattt aaaagaaaaa aacctaggaa
     3121 tgtaggatgg caaagatgat atttgcttct cgtggcgtta attcagtatt gggagagtca
     3181 ggcggtgtgg ggggcattcc caaatgggaa atcattctta acttagaaga ctctttttca
     3241 gattcttgtt actccaggga gactagtttt acctttgagc cccagcaatg ggtagtttca
     3301 gctgccaggg gctgcaatcc accttgtcct cagtgttttc cactctaggg attcaggaca
     3361 agtctgtcca cgtctccttg gcgtgggtgt ttgtccagtg aacagacact cagttctaat
     3421 ctcatgtgtc tgcatttgtt tctgagtcta tatttgcttt ctcagagcat agcaaggtca
     3481 actcctaaaa atgtacccag cagaagacct ttttgaaaaa ccacacactt gcttcctctg
     3541 tattcacaca caaagtggtg gtatcataca ttatgccctg tgatgtaagt ccccttagtg
     3601 ctcaaagctg cctttgtctc ttaagagcgt gcactgagaa aggctgtggg aacggaaggc
     3661 ttattctcag acagggtggt ttgtttaccc catgtctgga cttgtcctga gtctgaggac
     3721 cactctgctg cactgcccgt cctgattaaa gacgtctctg tagatgctat tttctgtctc
     3781 aatttacaca ttctttcatc accttaaatc taagaactgg gtactctaat ggattgatag
     3841 agaccaagag agacgtggtc aagggaagat taaatcggga catgtgtgga gaaccaggaa
     3901 ggcctccatg tgagctctgt tccactcgag ctctgcttgc actagaggtg ggttgtgatt
     3961 atcagggcgt gctggtctgc tctgttctcc gttgtacata gaaggtagct acaaggacac
     4021 agtggcatgt ctttaccgcc accaccagga gctaaggcag aaagattgag aactcaaggc
     4081 cagctgggct aaacagtgag accttgtaca aaaa
//
