LOCUS       NM_025842                944 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus vacuolar protein sorting 28 (Vps28), transcript
            variant 1, mRNA.
ACCESSION   NM_025842
VERSION     NM_025842.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Hwang,S.G., Shim,J. and Choi,E.J.
  TITLE     CIIA negatively regulates neuronal cell death induced by
            oxygen-glucose deprivation and reoxygenation
  JOURNAL   Mol Cell Biochem 397 (1-2), 139-146 (2014)
   PUBMED   25098452
  REMARK    GeneRIF: CIIA attenuates neurotoxicity caused by OGD/R through
            inhibiting ASK1-dependent signaling events.
REFERENCE   2  (bases 1 to 944)
  AUTHORS   Hwang,H.S., Hwang,S.G., Cho,J.H., Chae,J.S., Yoon,K.W., Cho,S.G.
            and Choi,E.J.
  TITLE     CIIA functions as a molecular switch for the Rac1-specific GEF
            activity of SOS1
  JOURNAL   J Cell Biol 195 (3), 377-386 (2011)
   PUBMED   22042618
  REMARK    GeneRIF: CIIA functions as a molecular switch for the GEF activity
            of SOS1, directing this activity toward Rac1.
REFERENCE   3  (bases 1 to 944)
  AUTHORS   Cho,S.G., Kim,J.W., Lee,Y.H., Hwang,H.S., Kim,M.S., Ryoo,K.,
            Kim,M.J., Noh,K.T., Kim,E.K., Cho,J.H., Yoon,K.W., Cho,E.G.,
            Park,H.S., Chi,S.W., Lee,M.J., Kang,S.S., Ichijo,H. and Choi,E.J.
  TITLE     Identification of a novel antiapoptotic protein that antagonizes
            ASK1 and CAD activities
  JOURNAL   J Cell Biol 163 (1), 71-81 (2003)
   PUBMED   14557248
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY083019.1, AK003699.1,
            AK013852.1 and AC157566.6.
            
            On Mar 27, 2015 this sequence version replaced NM_025842.3.
            
            Summary: This gene encodes a protein which is thought to be a
            subunit of the ESCRT-I complex (endosomal complexes required for
            transport), which functions in the transport and sorting of
            proteins into subcellular vesicles. This complex can also be
            hijacked to facilitate the budding of enveloped viruses from the
            cell membrane. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Mar
            2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU557705.1, AF373710.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                BY083019.1         3-59
            58-172              AK003699.1         2-116
            173-173             AK013852.1         114-114
            174-942             AK003699.1         118-886
            943-944             AC157566.6         14300-14301
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 36.17 cM"
     gene            1..944
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /note="vacuolar protein sorting 28"
                     /db_xref="GeneID:66914"
                     /db_xref="MGI:MGI:1914164"
     exon            1..96
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            97..168
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..797
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 28 homolog;
                     ESCRT-I complex subunit VPS28; caspase-activated DNase
                     inhibitor that interacts with ASK1"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 28
                     homolog isoform 1"
                     /protein_id="NP_080118.1"
                     /db_xref="CCDS:CCDS27579.1"
                     /db_xref="GeneID:66914"
                     /db_xref="MGI:MGI:1914164"
                     /translation="MFHGIPATPGVGAPGNKPELYEEVKLYKNAREREKYDNMAELFA
                     VVKTMQALEKAYIKDCVTPNEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLD
                     CPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLREL
                     METMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRF
                     LHA"
     exon            169..197
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            198..235
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            236..325
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            326..431
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            432..533
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            534..587
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            588..679
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            680..944
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      900..905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      937
                     /gene="Vps28"
                     /gene_synonym="1110014J03Rik; CIIA; D730005C08Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagtgccgag ctgccgatcg ggtttcccgg caggcgcagg cggaaaaagc gagcaccgag
       61 cttctgtcgc catctccggg actccaaacg ccccaggtct cttgtgctgt atagctgcct
      121 gggcctgcag gatgttccac gggatcccag ctactcctgg tgttggagcc cctgggaaca
      181 agccggagct gtatgaggaa gtaaagctct acaagaatgc tcgggagcgg gagaagtatg
      241 acaacatggc agagctcttt gccgtggtga agacgatgca ggccctggag aaggcgtaca
      301 tcaaggactg tgtcaccccc aatgagtaca ctgcagcctg ctccaggctc ctggtccagt
      361 acaaagctgc cttccgacag gtccaaggct cagagatcag ctccattgat gaattttgcc
      421 gaaagttcag actggactgc ccacttgcta tggagaggat caaagaggac cggcccatca
      481 ctatcaaaga cgacaagggc aatctcaacc gctgcattgc agatgttgtt tcgctcttca
      541 ttacagtcat ggacaagctg cgtctggaga tccgtgccat ggacgagatt cagccagacc
      601 tgcgggagct gatggagaca atgcacagaa tgagccacct gcctccagac ttcgagggcc
      661 gccagacagt cagccagtgg ctgcagaccc tgagtggtat gtcggcctct gacgagctgg
      721 atgactctca agttcgccag atgctcttcg atctggagtc cgcttacaac gcctttaacc
      781 gcttcctaca cgcctaagcc tcaccgagac aggaatgaga gtggtagaga tgtgacgact
      841 cagcccccca gtgtgtctac atccgtccta gatgcctata ttgtcaggat atcacccaca
      901 ataaatattt gtctaacctt cctgctgtgg gcagctatac tgag
//
