LOCUS       NM_026776               1108 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus vacuolar protein sorting 25 (Vps25), transcript
            variant 2, mRNA.
ACCESSION   NM_026776
VERSION     NM_026776.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Hermosilla Aguayo,V., Martin,P., Tian,N., Zheng,J., Aho,R., Losa,M.
            and Selleri,L.
  TITLE     ESCRT-dependent control of craniofacial morphogenesis with
            concomitant perturbation of NOTCH signaling
  JOURNAL   Dev Biol 503, 25-42 (2023)
   PUBMED   37573008
REFERENCE   3  (bases 1 to 1108)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1108)
  AUTHORS   Handschuh,K., Feenstra,J., Koss,M., Ferretti,E., Risolino,M.,
            Zewdu,R., Sahai,M.A., Benazet,J.D., Peng,X.P., Depew,M.J.,
            Quintana,L., Sharpe,J., Wang,B., Alcorn,H., Rivi,R., Butcher,S.,
            Manak,J.R., Vaccari,T., Weinstein,H., Anderson,K.V., Lacy,E. and
            Selleri,L.
  TITLE     ESCRT-II/Vps25 constrains digit number by endosome-mediated
            selective modulation of FGF-SHH signaling
  JOURNAL   Cell Rep 9 (2), 674-687 (2014)
   PUBMED   25373905
  REMARK    GeneRIF: Vps25(ENU/ENU) mutants survive until late gestation. Their
            limbs display FGF signaling enhancement and consequent
            hyperactivation of the FGF-SHH feedback loop causing polydactyly.
REFERENCE   5  (bases 1 to 1108)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1108)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 1108)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590969.12 and AK150822.1.
            
            On Apr 28, 2018 this sequence version replaced NM_026776.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI646344.1, BC029181.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AL590969.12        135801-135819       c
            20-1102             AK150822.1         2-1084
            1103-1108           AL590969.12        129975-129980       c
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 64.48 cM"
     gene            1..1108
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /note="vacuolar protein sorting 25"
                     /db_xref="GeneID:28084"
                     /db_xref="MGI:MGI:106354"
     exon            1..101
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..579
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /note="isoform 2 is encoded by transcript variant 2;
                     vacuolar protein-sorting-associated protein 25; ESCRT-II
                     complex subunit VPS25"
                     /codon_start=1
                     /product="vacuolar protein-sorting-associated protein 25
                     isoform 2"
                     /protein_id="NP_081052.2"
                     /db_xref="CCDS:CCDS25458.1"
                     /db_xref="GeneID:28084"
                     /db_xref="MGI:MGI:106354"
                     /translation="MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLH
                     KQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWR
                     RPEEWGKLIYQWVSRSGQNNSVFTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEH
                     KAEIITVSDGRGVKFF"
     exon            102..247
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     exon            248..301
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     exon            302..390
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     exon            391..466
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     exon            467..1108
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1085..1090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /note="hexamer: ATTAAA"
     polyA_site      1103
                     /gene="Vps25"
                     /gene_synonym="1110020N13Rik; D11Wsu68e"
                     /note="major polyA site"
ORIGIN      
        1 cggagaccgg aagccccttg tcggttccgg gttttctggg ctactacgat ggcgatgagt
       61 ttcgagtggc cgtggcagta ccgcttcccg cccttcttta cgttacagcc gaacgtggac
      121 acccggcaga agcagctggc cgcctggtgc tctctggttc tgtccttctg ccgcctgcac
      181 aaacagtcca gcatgacggt gatggaagcc caggagagcc cgcttttcaa caacgtcaag
      241 ctacagcgga aacttcctgt ggagtcaatt cagattgtat tagaagaact gagaaagaaa
      301 gggaacctcg agtggttgga taagaataag tctagcttcc tgatcatgtg gcggaggcca
      361 gaagaatggg ggaaactcat ttaccaatgg gtttccagga gtggccagaa taactctgtg
      421 tttactctgt atgaactgac gagtggggaa gacacagagg atgaagagtt ccatgggctg
      481 gacgaggcga ccctgctgcg cgctctgcag gccctacagc aagagcacaa ggctgagatt
      541 atcactgtca gcgatggccg aggagtcaag ttcttctagc agagacctgg ctcctttact
      601 tcttacctcc cacctttcca gggctttcaa aaggagacag acctagtgtc cccacagact
      661 ggatctgtca ctcctccaga ctcaaagggc tcaagtcctg aaagctcaga cttagggatg
      721 tttcccacat aaccctttat ttctgtccct gggatagagt aagcccacag tgccacggag
      781 aaaggatgcg tttcttgccc tacctccttt cccatcctat actgtccccg agccagggtc
      841 tataaacctg acactttaca tgtgttctca cacataagta cacacccacc tacatgcgcc
      901 tgcacaagct tctttctcct cctcccactc ctttagctgc tgttgcctcc cttctcaggc
      961 tggtgctgga tcctccccag ggatggggga agccctggct gcaggcagcc ggccaggcag
     1021 tatgaagata ggaagcccca gaaggggcct ggtggtgaga ggcgggccca ctctgattta
     1081 tgatattaaa atctctactc ccactgtc
//
