LOCUS       NM_030559               3206 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus VSP16 CORVET/HOPS core subunit (Vps16), transcript
            variant 1, mRNA.
ACCESSION   NM_030559
VERSION     NM_030559.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Cai,X., Chen,X., Wu,S., Liu,W., Zhang,X., Zhang,D., He,S., Wang,B.,
            Zhang,M., Zhang,Y., Li,Z., Luo,K., Cai,Z. and Li,W.
  TITLE     Homozygous mutation of VPS16 gene is responsible for an autosomal
            recessive adolescent-onset primary dystonia
  JOURNAL   Sci Rep 6, 25834 (2016)
   PUBMED   27174565
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Perini,E.D., Schaefer,R., Stoter,M., Kalaidzidis,Y. and Zerial,M.
  TITLE     Mammalian CORVET is required for fusion and conversion of distinct
            early endosome subpopulations
  JOURNAL   Traffic 15 (12), 1366-1389 (2014)
   PUBMED   25266290
REFERENCE   3  (bases 1 to 3206)
  AUTHORS   Pirooz,S.D., He,S., Zhang,T., Zhang,X., Zhao,Z., Oh,S.,
            O'Connell,D., Khalilzadeh,P., Amini-Bavil-Olyaee,S., Farzan,M. and
            Liang,C.
  TITLE     UVRAG is required for virus entry through combinatorial interaction
            with the class C-Vps complex and SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2716-2721 (2014)
   PUBMED   24550300
REFERENCE   4  (bases 1 to 3206)
  AUTHORS   Zlatic,S.A., Tornieri,K., L'Hernault,S.W. and Faundez,V.
  TITLE     Clathrin-dependent mechanisms modulate the subcellular distribution
            of class C Vps/HOPS tether subunits in polarized and nonpolarized
            cells
  JOURNAL   Mol Biol Cell 22 (10), 1699-1715 (2011)
   PUBMED   21411634
REFERENCE   5  (bases 1 to 3206)
  AUTHORS   Zhu,G.D., Salazar,G., Zlatic,S.A., Fiza,B., Doucette,M.M.,
            Heilman,C.J., Levey,A.I., Faundez,V. and L'hernault,S.W.
  TITLE     SPE-39 family proteins interact with the HOPS complex and function
            in lysosomal delivery
  JOURNAL   Mol Biol Cell 20 (4), 1223-1240 (2009)
   PUBMED   19109425
REFERENCE   6  (bases 1 to 3206)
  AUTHORS   Sacksteder,C.A., Qian,W.J., Knyushko,T.V., Wang,H., Chin,M.H.,
            Lacan,G., Melega,W.P., Camp,D.G. 2nd, Smith,R.D., Smith,D.J.,
            Squier,T.C. and Bigelow,D.J.
  TITLE     Endogenously nitrated proteins in mouse brain: links to
            neurodegenerative disease
  JOURNAL   Biochemistry 45 (26), 8009-8022 (2006)
   PUBMED   16800626
REFERENCE   7  (bases 1 to 3206)
  AUTHORS   Richardson,S.C., Winistorfer,S.C., Poupon,V., Luzio,J.P. and
            Piper,R.C.
  TITLE     Mammalian late vacuole protein sorting orthologues participate in
            early endosomal fusion and interact with the cytoskeleton
  JOURNAL   Mol Biol Cell 15 (3), 1197-1210 (2004)
   PUBMED   14668490
REFERENCE   8  (bases 1 to 3206)
  AUTHORS   Kim,B.Y., Ueda,M., Nakamura,Y., Kohsaka,S. and Akazawa,C.
  TITLE     Expression of the mammalian homologue of vacuolar protein sorting
            16 (Vps16p) in the mouse and rat brain
  JOURNAL   Neurosci Lett 355 (3), 217-220 (2004)
   PUBMED   14732470
  REMARK    GeneRIF: Vps16p is highly expressed in neurons of various brain
            areas and developmental stages tested; it colocalizes with
            microtubule associated protein 2, but not glial fibrillary acidic
            protein.
REFERENCE   9  (bases 1 to 3206)
  AUTHORS   Kim,B.Y., Ueda,M., Kominami,E., Akagawa,K., Kohsaka,S. and
            Akazawa,C.
  TITLE     Identification of mouse Vps16 and biochemical characterization of
            mammalian class C Vps complex
  JOURNAL   Biochem Biophys Res Commun 311 (3), 577-582 (2003)
   PUBMED   14623309
  REMARK    GeneRIF: mVps16p has potential roles in membrane trafficking
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY123989.1, AK049872.1, BX890605.4 and AK032558.1.
            
            On Jul 31, 2009 this sequence version replaced NM_030559.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049872.1, AB056721.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                BY123989.1         2-46
            46-132              AK049872.1         3-89
            133-133             BX890605.4         131507-131507
            134-2667            AK049872.1         91-2624
            2668-3077           AK032558.1         1627-2036
            3078-3078           BX890605.4         138022-138022
            3079-3206           AK032558.1         2038-2165
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 63.22 cM"
     gene            1..3206
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /note="VSP16 CORVET/HOPS core subunit"
                     /db_xref="GeneID:80743"
                     /db_xref="MGI:MGI:2136772"
     exon            1..128
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2595
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vacuolar protein sorting-associated protein 16 homolog;
                     vacuolar protein sorting 16"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 16
                     homolog isoform 1"
                     /protein_id="NP_085036.3"
                     /db_xref="CCDS:CCDS38242.1"
                     /db_xref="GeneID:80743"
                     /db_xref="MGI:MGI:2136772"
                     /translation="MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYG
                     GPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQE
                     DGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVG
                     DLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPG
                     VSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRS
                     KERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVAS
                     EEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQ
                     PDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTI
                     QVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAIN
                     QKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAI
                     ESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDD
                     NHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRR
                     LEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDW
                     EELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAA
                     EVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK"
     exon            129..217
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            218..315
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            316..444
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            445..589
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            590..705
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            706..828
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            829..884
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            885..974
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1069
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1146
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1278
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1406
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1442
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1551
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1686
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1795
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1893
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2001
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2079
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2249
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2346
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2450
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..3206
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2646..2651
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /note="hexamer: AATAAA"
     polyA_site      2671
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /note="major polyA site"
     regulatory      3175..3180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
                     /note="hexamer: AATGAA"
     polyA_site      3192
                     /gene="Vps16"
                     /gene_synonym="1810074M16Rik; mVPS16"
ORIGIN      
        1 ggaagcggaa gtgcggcctg agcttgcgct aggcgggttt ttcctcggcg actcttgccg
       61 ccagctgtac tagacatgga ctgttacact gcgaactgga atccactcgg ggactctgcc
      121 ttttacagga agtatgagct gtacagcatg gactgggacc tgaaggagga gctcaaggat
      181 tgcctggtgg ctgctgcacc ctatgggggt cccattgctc tactgaggaa ctgttggaga
      241 aaagagaaag ctgccagcgt gcggccagta ctggagatct actctgcatc tggcttacca
      301 ctggctagcc tgctgtggaa gagtgggcct gtggtggccc tcggctggtc agctgaggag
      361 gagctgctct gtgtgcaaga agatggtgca gtgctagttt atggccttca tggagacttc
      421 cggaggcact tcagcatggg caatgaagtg cttcagaacc gtgtcctgga tgcccggatt
      481 tttcacactg agtttggttc aggggtggcc atccttacag gagcctatcg ctttactctg
      541 agtgccaatg tgggtgacct caaactccgt cggatgccag aggtgccagg tctgcaaagt
      601 gcaccctcat gttggaccac actgtgccac gaccgagtac cacacattct tctggctgta
      661 ggacccgatc tttaccttct ggatcatgct acctgctctg cagtgacacc tgctggctta
      721 gccccaggag tgagcagctt cctacagatg gctgtatcct tcacgtatcg ttacctggca
      781 ctcttcacag acacaggcta catctggatg gggacagcat cactcaagga gaagctgtgt
      841 gagttcaact gcaacatccg ggctcccccg aagcagatgg tctggtgtag ccgtcctcgg
      901 agcaaggaaa gggctgttgt tgtggcctgg gagaggcggc tgatggtggt gggcaatgcg
      961 cctgaaagca tccagtttgt gctcgatgag gactcctact tagtgcctga gcttgatggg
     1021 gtccgcatct tctcccgaag cacccatgag ttcctgcatg aagtcccagt ggccagtgaa
     1081 gagatcttca aaattgcctc aatggcccct ggagcactgc tgttagaggc ccagaaggaa
     1141 tatgagaaag agagccagaa agcagatgag tacctgcggg agatccagga actgggccag
     1201 ctgatccagg ctgtgcagca gtgcattgag gctgcaggac atgagcacca gccagacatg
     1261 caaaagagtc tgctcagggc ggcttcattt ggaaagtgtt tccttgacag atttccacct
     1321 gacagcttcg tgcacatgtg tcaggacctt cgagtgctca atgctattag agactaccac
     1381 attgggatcc ctctcaccta cacccaatac aagcagctca ccatccaggt gctgctagac
     1441 aggcttgtgt tgcggaggct ttatcccctg gctatccaga tatgcgagta cctgcgcctt
     1501 cctgaagtcc agggtgtcag caggatccta gcccactggg cctgctacaa ggtgcagcag
     1561 aaagatgtct cagatgaaga tgtagcacgg gctattaatc agaagctggg tgacacgcct
     1621 ggtgtttcat actctgacat tgctgcgagg gcctatggct gtggccgcac tgaactggcc
     1681 attaagctgc tggagtatga gccacgctca ggggagcagg tacctcttct cctaaagatg
     1741 aagaggagca aactggcact gagcaaggct attgagagtg gagacactga cctggtgttt
     1801 acagtgttgc tgcacctaaa gaatgaactc aaccgaggag actttttcat gactctccgg
     1861 aaccaaccca tggccctgag tttgtatcga cagttctgta agcatcagga gctagacact
     1921 ctgaaggacc tttacaatca ggacgacaat caccaggagt tgggcagctt ccacatcaga
     1981 gccagctatg ccgctgaaga gcgtattgaa gggcgagtgg cagctctgca gacggcagct
     2041 gatgccttct acaaagccaa gaatgaattt gcagccaagg ccacagagga tcaaatgagg
     2101 ctcctgcgga tacagcgacg cctagaggat gagctagggg gcaggttcct agacctatct
     2161 ctacacgaca ctgtcaccac tcttatcctt ggaggccaca acaagcgggc agagcagctg
     2221 gcacgtgact ttcgcatccc tgataagaga ctatggtggt taaagctggc tgccctggca
     2281 gatttggaag attgggagga gctggagaag ttttccaaaa gcaagaaatc accgattggt
     2341 tacctgccct ttgtagagat ctgcatgaaa cagcataaca aacatgaggc caagaagtat
     2401 gcttcccgtg tgggccctga acaaaaggtc aaggctttgc ttcttgttgg ggatgtggct
     2461 caggctgcag aggtggctat tgagcaccgg aatgaaacag agctgagcct cgttttgtcc
     2521 cactgcactg gagccacaga tggggccata gctgacaaga ttcagcgggc cagggcacaa
     2581 gcccagaaga agtgaagagc ctgtccttaa ggggactagt taaaacccca ggatgtccag
     2641 atgtaaataa atttggaata ctaattcaga atagttgttg tatgctgtct ggcttaagaa
     2701 aagcaggctt tgaggcaaaa ctgtctagct actgcagcag aactctgcat atttgattgt
     2761 attgaggttg ggaataggaa gttataagag atctgtttat tcacgtagtc accatagtaa
     2821 ttctctcaga agctgtctca cagcctggag gaaggtgtgg tgggagatgg cataatattc
     2881 aagcaccacg gtacccggga agccctacac tgaagagcat gacatgcctg cccatgagct
     2941 tacaaggacc acactgtgca cttcgctatt attcacccat ccctcacaac agttctgaat
     3001 ctataacagc aaatacgtat caaaagaagg atttgggctg gccaggtggc tcagtagcta
     3061 aaggcttttg ccaccaagcc aggcaacttg agtttaaacc cttctcctct gatctttacc
     3121 catatacaaa acatactgaa tagatgggtg tatgaatgaa tgaatggatg aatgaatgaa
     3181 aataaataga aataaatgag gatttg
//
